Standard name
Human Ortholog
Description E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.98 0.99 0.94 0.88 0.84 0.85 0.77 0.8 0.81 1.0 0.99 1.0 0.96 1.0 0.98 0.97 0.96 0.95 0.91 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.16 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 3 4 1 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 1 1 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 2 1 2 7 11 19 9 15 9 22 2 9 8 1 1 0 0 0 0 0 0 0
Cytoplasm 135 85 109 205 136 285 327 422 332 232 273 321 193 300 330 25 82 85 129 95 109 50 245 162
Endoplasmic Reticulum 0 0 1 1 1 11 18 33 14 7 12 16 0 1 0 1 3 3 0 0 0 0 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 1 0 11 5 18 12 0 0 0 0 0 0 0 0 0 0 1 1
Nucleus 0 0 1 2 0 5 8 13 10 6 6 10 0 2 1 1 0 1 1 0 1 0 6 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 3 1 4 15 33 27 47 41 31 0 1 1 0 0 0 0 1 0 0 0 0
Unique Cell Count 136 85 110 209 137 302 371 501 390 302 342 396 193 304 331 26 82 87 133 100 116 56 260 170
Labelled Cell Count 137 86 114 213 140 312 383 523 408 318 361 422 195 314 340 28 86 90 133 100 116 56 260 170


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 19.2 17.6 18.4 19.7 17.4 16.1 15.8 13.9 14.3 13.3 13.6 19.7 21.5 20.2 21.8 27.2 26.8 14.5 20.6 22.4
Std Deviation (1e-4) 4.3 5.5 4.5 3.1 3.0 3.2 2.9 2.8 2.6 3.4 2.7 2.8 4.1 3.8 3.7 5.9 9.0 8.3 3.4 4.6 5.1
Intensity Change (Log2) 0.06 0.16 -0.02 -0.13 -0.16 -0.35 -0.3 -0.41 -0.38 0.16 0.29 0.2 0.31 0.62 0.61 -0.29 0.23 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.6 0 0 0 0 0 0
Cytoplasm -0.7 0.2 -2.1 -3.5 -4.2 -4.0 -5.3 -4.9 -4.7 1.3 -0.3 0.8 -1.1 0.9 -0.8
Endoplasmic Reticulum 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.4 2.4 4.1 3.5 2.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.8717 14.4811 13.7489 13.2042 13.2713 13.4433 11.9371 17.4496 15.1554 14.747 13.4703 15.1411 11.607 15.131 13.3479 12.835 11.8682 13.3456
Actin 0.0136 0.0004 0.0004 0.0002 0.0002 0.0018 0.0246 0.0031 0.0203 0.0146 0.0083 0.0062 0.0034 0.0003 0.0068 0.0076 0.0744 0.0027
Bud 0.0001 0.0001 0 0 0 0.0002 0.0017 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0 0.0003 0.0004 0.0037 0.0001
Bud Neck 0.0008 0.0002 0.0003 0.0003 0.0005 0.001 0.001 0.0001 0.0082 0.0008 0.0004 0.0011 0.0003 0.0002 0.0016 0.0007 0.0009 0.0011
Bud Periphery 0.0001 0 0 0 0 0.0001 0.0021 0.0002 0.0002 0.0001 0.0002 0 0.0001 0 0.0003 0.0005 0.0015 0.0001
Bud Site 0.0003 0.0001 0.0001 0 0 0.0001 0.0044 0.0004 0.0028 0.0003 0.0002 0.0001 0.0003 0.0001 0.001 0.0009 0.0028 0.0006
Cell Periphery 0.0001 0 0 0 0 0 0.0008 0 0.0001 0 0 0 0 0 0.0001 0.0001 0.001 0
Cytoplasm 0.94 0.9834 0.9927 0.9952 0.9906 0.9724 0.7646 0.9747 0.9185 0.9577 0.8801 0.9705 0.9445 0.997 0.9477 0.9491 0.8512 0.9738
Cytoplasmic Foci 0.0099 0.0026 0.0009 0.0003 0.001 0.0013 0.0434 0.0005 0.0065 0.014 0.0299 0.0085 0.0076 0.0004 0.011 0.0157 0.005 0.0028
Eisosomes 0.0001 0 0 0 0 0 0.0023 0 0.0002 0 0 0 0 0 0 0 0.0005 0.0002
Endoplasmic Reticulum 0.0021 0.0008 0.0006 0.0004 0.0019 0.0009 0.0076 0.0002 0.0011 0.0007 0.0008 0.002 0.0033 0.0002 0.0009 0.0016 0.001 0.0007
Endosome 0.0095 0.0045 0.0004 0.0001 0.0006 0.0025 0.0419 0.0006 0.0067 0.0007 0.036 0.0023 0.0158 0 0.009 0.0052 0.0026 0.0012
Golgi 0.0042 0.0009 0 0 0 0.0009 0.0227 0.0012 0.0211 0.0006 0.0328 0.001 0.0036 0 0.0025 0.0014 0.0043 0.0009
Lipid Particles 0.003 0.0013 0 0 0 0.001 0.0193 0.0001 0.0016 0 0.0042 0.0004 0.0009 0 0.0009 0.0088 0.003 0.0017
Mitochondria 0.0011 0.0006 0 0 0 0.0073 0.0201 0.0166 0.0017 0.0007 0.0016 0.0002 0.0005 0 0.0105 0.0008 0.013 0.0007
None 0.0033 0.0008 0.0012 0.0003 0.0008 0.0007 0.0151 0.0005 0.003 0.0025 0.0007 0.0024 0.0057 0.0004 0.0014 0.0009 0.003 0.0041
Nuclear Periphery 0.0019 0.0005 0.0005 0.0003 0.0009 0.0023 0.0038 0.0001 0.0004 0.0004 0.0003 0.0006 0.0043 0.0002 0.0004 0.0006 0.0012 0.0011
Nucleolus 0.0003 0 0 0 0 0.0001 0.0001 0 0.0003 0 0 0 0.0001 0 0.0001 0 0.0093 0.001
Nucleus 0.0035 0.0026 0.0025 0.0025 0.0026 0.0063 0.0025 0.0008 0.0018 0.0029 0.0018 0.0026 0.0056 0.0011 0.0021 0.0023 0.0095 0.0044
Peroxisomes 0.0018 0.0003 0 0 0 0.0001 0.0154 0.0003 0.0026 0.0031 0.0014 0.0011 0.0008 0 0.0016 0.0018 0.0073 0.0006
Punctate Nuclear 0.0037 0.0001 0.0002 0.0001 0.0002 0.0005 0.0026 0 0.0026 0.0006 0.0003 0.0005 0.0018 0.0001 0.0007 0.0008 0.0008 0.0018
Vacuole 0.0004 0.0004 0.0002 0.0001 0.0004 0.0004 0.0028 0.0001 0.0001 0.0001 0.0003 0.0003 0.001 0 0.0008 0.0005 0.0023 0.0002
Vacuole Periphery 0.0002 0.0002 0 0 0.0001 0.0003 0.0013 0.0001 0.0001 0 0.0003 0 0.0003 0 0.0004 0.0001 0.0018 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.6788 72.5905 66.5394 79.2835 46.4484 69.223 85.269 83.0481 73.0112 79.3594
Translational Efficiency 1.9837 2.1205 1.9148 1.6502 2.6854 2.5224 2.1949 2.045 2.249 2.0271

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ubc13

Ubc13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubc13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available