Standard name
Description E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.98 0.99 0.94 0.88 0.84 0.85 0.77 0.8 0.81 1.0 0.99 1.0 0.96 1.0 0.98 0.97 0.96 0.95 0.91 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.07 0.16 0.12 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 0 3 4 1 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 1 1 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 1 2 7 11 19 9 15 9 22 2 9 8 1 1 0 0 0 0 0 0 0
Cytoplasm 135 85 109 205 136 285 327 422 332 232 273 321 193 300 330 25 82 85 129 95 109 50 245 162
Endoplasmic Reticulum 0 0 1 1 1 11 18 33 14 7 12 16 0 1 0 1 3 3 0 0 0 0 1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 1 0 11 5 18 12 0 0 0 0 0 0 0 0 0 0 1 1
Nucleus 0 0 1 2 0 5 8 13 10 6 6 10 0 2 1 1 0 1 1 0 1 0 6 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 3 1 4 15 33 27 47 41 31 0 1 1 0 0 0 0 1 0 0 0 0
Unique Cell Count 136 85 110 209 137 302 371 501 390 302 342 396 193 304 331 26 82 87 133 100 116 56 260 170
Labelled Cell Count 137 86 114 213 140 312 383 523 408 318 361 422 195 314 340 28 86 90 133 100 116 56 260 170


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 19.2 17.6 18.4 19.7 17.4 16.1 15.8 13.9 14.3 13.3 13.6 19.7 21.5 20.2 21.8 27.2 26.8 14.5 20.6 22.4
Std Deviation (1e-4) 4.3 5.5 4.5 3.1 3.0 3.2 2.9 2.8 2.6 3.4 2.7 2.8 4.1 3.8 3.7 5.9 9.0 8.3 3.4 4.6 5.1
Intensity Change (Log2) – – – 0.06 0.16 -0.02 -0.13 -0.16 -0.35 -0.3 -0.41 -0.38 0.16 0.29 0.2 0.31 0.62 0.61 -0.29 0.23 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.6 0 0 0 0 0 0
Cytoplasm -0.7 0.2 -2.1 -3.5 -4.2 -4.0 -5.3 -4.9 -4.7 1.3 -0.3 0.8 -1.1 0.9 -0.8
Endoplasmic Reticulum 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.4 2.4 4.1 3.5 2.6 0 0 0 0 0 0