Standard name | |
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Human Ortholog | |
Description | E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 4 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 1 | 2 | 7 | 11 | 19 | 9 | 15 | 9 | 22 | 2 | 9 | 8 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 135 | 85 | 109 | 205 | 136 | 285 | 327 | 422 | 332 | 232 | 273 | 321 | 193 | 300 | 330 | 25 | 82 | 85 | 129 | 95 | 109 | 50 | 245 | 162 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 1 | 11 | 18 | 33 | 14 | 7 | 12 | 16 | 0 | 1 | 0 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 11 | 5 | 18 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 1 | 2 | 0 | 5 | 8 | 13 | 10 | 6 | 6 | 10 | 0 | 2 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 6 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 3 | 1 | 4 | 15 | 33 | 27 | 47 | 41 | 31 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Unique Cell Count | 136 | 85 | 110 | 209 | 137 | 302 | 371 | 501 | 390 | 302 | 342 | 396 | 193 | 304 | 331 | 26 | 82 | 87 | 133 | 100 | 116 | 56 | 260 | 170 |
Labelled Cell Count | 137 | 86 | 114 | 213 | 140 | 312 | 383 | 523 | 408 | 318 | 361 | 422 | 195 | 314 | 340 | 28 | 86 | 90 | 133 | 100 | 116 | 56 | 260 | 170 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.7 | 19.2 | 17.6 | 18.4 | 19.7 | 17.4 | 16.1 | 15.8 | 13.9 | 14.3 | 13.3 | 13.6 | 19.7 | 21.5 | 20.2 | 21.8 | 27.2 | 26.8 | 14.5 | 20.6 | 22.4 |
Std Deviation (1e-4) | 4.3 | 5.5 | 4.5 | 3.1 | 3.0 | 3.2 | 2.9 | 2.8 | 2.6 | 3.4 | 2.7 | 2.8 | 4.1 | 3.8 | 3.7 | 5.9 | 9.0 | 8.3 | 3.4 | 4.6 | 5.1 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.16 | -0.02 | -0.13 | -0.16 | -0.35 | -0.3 | -0.41 | -0.38 | 0.16 | 0.29 | 0.2 | 0.31 | 0.62 | 0.61 | -0.29 | 0.23 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 0.2 | -2.1 | -3.5 | -4.2 | -4.0 | -5.3 | -4.9 | -4.7 | 1.3 | -0.3 | 0.8 | -1.1 | 0.9 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.4 | 2.4 | 4.1 | 3.5 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.8717 | 14.4811 | 13.7489 | 13.2042 | 13.2713 | 13.4433 | 11.9371 | 17.4496 | 15.1554 | 14.747 | 13.4703 | 15.1411 | 11.607 | 15.131 | 13.3479 | 12.835 | 11.8682 | 13.3456 |
Actin | 0.0136 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0018 | 0.0246 | 0.0031 | 0.0203 | 0.0146 | 0.0083 | 0.0062 | 0.0034 | 0.0003 | 0.0068 | 0.0076 | 0.0744 | 0.0027 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0037 | 0.0001 |
Bud Neck | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.001 | 0.001 | 0.0001 | 0.0082 | 0.0008 | 0.0004 | 0.0011 | 0.0003 | 0.0002 | 0.0016 | 0.0007 | 0.0009 | 0.0011 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0021 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0005 | 0.0015 | 0.0001 |
Bud Site | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0044 | 0.0004 | 0.0028 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.001 | 0.0009 | 0.0028 | 0.0006 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.001 | 0 |
Cytoplasm | 0.94 | 0.9834 | 0.9927 | 0.9952 | 0.9906 | 0.9724 | 0.7646 | 0.9747 | 0.9185 | 0.9577 | 0.8801 | 0.9705 | 0.9445 | 0.997 | 0.9477 | 0.9491 | 0.8512 | 0.9738 |
Cytoplasmic Foci | 0.0099 | 0.0026 | 0.0009 | 0.0003 | 0.001 | 0.0013 | 0.0434 | 0.0005 | 0.0065 | 0.014 | 0.0299 | 0.0085 | 0.0076 | 0.0004 | 0.011 | 0.0157 | 0.005 | 0.0028 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0023 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0002 |
Endoplasmic Reticulum | 0.0021 | 0.0008 | 0.0006 | 0.0004 | 0.0019 | 0.0009 | 0.0076 | 0.0002 | 0.0011 | 0.0007 | 0.0008 | 0.002 | 0.0033 | 0.0002 | 0.0009 | 0.0016 | 0.001 | 0.0007 |
Endosome | 0.0095 | 0.0045 | 0.0004 | 0.0001 | 0.0006 | 0.0025 | 0.0419 | 0.0006 | 0.0067 | 0.0007 | 0.036 | 0.0023 | 0.0158 | 0 | 0.009 | 0.0052 | 0.0026 | 0.0012 |
Golgi | 0.0042 | 0.0009 | 0 | 0 | 0 | 0.0009 | 0.0227 | 0.0012 | 0.0211 | 0.0006 | 0.0328 | 0.001 | 0.0036 | 0 | 0.0025 | 0.0014 | 0.0043 | 0.0009 |
Lipid Particles | 0.003 | 0.0013 | 0 | 0 | 0 | 0.001 | 0.0193 | 0.0001 | 0.0016 | 0 | 0.0042 | 0.0004 | 0.0009 | 0 | 0.0009 | 0.0088 | 0.003 | 0.0017 |
Mitochondria | 0.0011 | 0.0006 | 0 | 0 | 0 | 0.0073 | 0.0201 | 0.0166 | 0.0017 | 0.0007 | 0.0016 | 0.0002 | 0.0005 | 0 | 0.0105 | 0.0008 | 0.013 | 0.0007 |
None | 0.0033 | 0.0008 | 0.0012 | 0.0003 | 0.0008 | 0.0007 | 0.0151 | 0.0005 | 0.003 | 0.0025 | 0.0007 | 0.0024 | 0.0057 | 0.0004 | 0.0014 | 0.0009 | 0.003 | 0.0041 |
Nuclear Periphery | 0.0019 | 0.0005 | 0.0005 | 0.0003 | 0.0009 | 0.0023 | 0.0038 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0006 | 0.0043 | 0.0002 | 0.0004 | 0.0006 | 0.0012 | 0.0011 |
Nucleolus | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0093 | 0.001 |
Nucleus | 0.0035 | 0.0026 | 0.0025 | 0.0025 | 0.0026 | 0.0063 | 0.0025 | 0.0008 | 0.0018 | 0.0029 | 0.0018 | 0.0026 | 0.0056 | 0.0011 | 0.0021 | 0.0023 | 0.0095 | 0.0044 |
Peroxisomes | 0.0018 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0154 | 0.0003 | 0.0026 | 0.0031 | 0.0014 | 0.0011 | 0.0008 | 0 | 0.0016 | 0.0018 | 0.0073 | 0.0006 |
Punctate Nuclear | 0.0037 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0026 | 0 | 0.0026 | 0.0006 | 0.0003 | 0.0005 | 0.0018 | 0.0001 | 0.0007 | 0.0008 | 0.0008 | 0.0018 |
Vacuole | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0028 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.001 | 0 | 0.0008 | 0.0005 | 0.0023 | 0.0002 |
Vacuole Periphery | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0003 | 0.0013 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0018 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 65.6788 | 72.5905 | 66.5394 | 79.2835 | 46.4484 | 69.223 | 85.269 | 83.0481 | 73.0112 | 79.3594 |
Translational Efficiency | 1.9837 | 2.1205 | 1.9148 | 1.6502 | 2.6854 | 2.5224 | 2.1949 | 2.045 | 2.249 | 2.0271 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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