Standard name
Human Ortholog
Description Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.1 0.06 0.09 0.05 0.05 0.08 0 – 0 0 0.05 0.09 0.14 – – 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.23 0.19 0 0.09 0 0.34 0.33 0.12 0.52 – 0.51 0.34 0.1 0 0 – – 0.21 0 0 0 0 0 0
Nucleus 0.87 0.88 0.82 0.91 0.85 0.77 0.84 0.83 0.82 – 0.78 0.83 0.87 0.86 0.77 – – 0.74 0.81 0.81 0.75 0.73 0.64 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 0 0.07 – 0.12 0.13 0.09 0 0.06 – – 0 0.05 0 0 0.05 0.05 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0.05 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0.21 0 0.07 0.09 0.16 0.15 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 2 1
Bud 0 0 0 0 0 2 2 3 2 – 5 5 0 0 0 – – 0 0 5 2 2 15 2
Bud Neck 0 0 6 0 2 1 1 3 0 – 3 2 0 0 0 – – 0 0 1 0 0 4 5
Bud Site 1 0 0 1 1 2 5 1 14 – 14 18 1 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 8 11 25 14 26 13 17 27 12 – 8 3 14 30 40 – – 2 2 1 3 1 4 2
Endoplasmic Reticulum 0 2 2 0 3 0 1 0 1 – 0 1 0 1 1 – – 1 1 0 1 0 0 2
Endosome 0 0 5 1 4 2 0 2 1 – 0 1 1 2 1 – – 0 3 3 2 1 2 6
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 2 1 1 1 1
Mitochondria 30 59 5 22 9 82 113 41 166 – 160 88 29 7 13 – – 4 1 7 6 1 5 6
Nucleus 112 280 207 231 239 186 287 281 261 – 246 216 243 274 227 – – 14 109 281 215 135 217 177
Nuclear Periphery 1 2 2 0 0 0 1 1 4 – 1 2 0 0 0 – – 0 0 0 0 0 1 0
Nucleolus 11 1 2 0 1 3 3 4 24 – 37 34 25 7 18 – – 0 6 9 11 8 18 25
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 – – 0 0 0 0 0 0 0
SpindlePole 1 3 19 13 8 10 5 10 6 – 6 11 3 2 0 – – 0 2 5 11 1 8 8
Vac/Vac Membrane 2 10 4 0 6 5 1 3 3 – 3 1 4 2 12 – – 4 5 25 25 28 50 38
Unique Cell Count 129 318 253 253 282 243 340 337 320 315 260 280 318 293 19 135 346 288 185 339 287
Labelled Cell Count 166 369 277 282 299 306 437 376 494 483 382 320 327 312 25 135 346 288 185 339 287


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.8 5.6 5.1 5.9 4.5 4.7 5.3 4.4 – 4.1 4.5 5.3 5.8 5.7 – – 7.6 4.9 5.1 5.6
Std Deviation (1e-4) 1.2 0.7 1.3 1.4 1.5 1.1 1.1 1.4 1.3 – 1.1 1.0 1.4 1.6 1.6 – – 1.8 1.6 1.8 1.6
Intensity Change (Log2) – – – -0.15 0.06 -0.32 -0.25 -0.09 -0.36 – -0.47 -0.32 -0.09 0.04 0.01 – – 0.44 -0.19 -0.14 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8684 3.041 3.0259 3.5202 2.246 2.9077 0.8941 1.6308 1.2977 1.431 0.6482 1.2294 1.795 2.0795 1.9443 1.9453 1.5315 2.0643
Actin 0.0078 0 0.0024 0.0011 0.0254 0.0078 0.0011 0 0.003 0.0217 0.0027 0.0001 0.0039 0 0.0002 0 0.0002 0
Bud 0.0002 0 0.0001 0.0002 0.0001 0.0002 0.0005 0 0.0004 0.0005 0.0003 0.0001 0.0002 0 0.0002 0 0.0001 0
Bud Neck 0.0014 0.0001 0.0004 0.0035 0.0023 0.0022 0.0011 0.0001 0.0012 0.0009 0.0004 0.0008 0.0014 0.0001 0.0002 0.0001 0.0008 0.0006
Bud Periphery 0.0004 0 0.0006 0.0003 0.0001 0.0007 0.0019 0 0.0005 0.0006 0.0009 0.0001 0.0004 0 0.0001 0 0.0003 0
Bud Site 0.0009 0 0.0033 0.0009 0.0002 0.0007 0.0016 0.0001 0.0072 0.0029 0.0006 0.0002 0.0008 0 0.0028 0 0.0004 0
Cell Periphery 0.0002 0 0.0037 0.0001 0 0.0001 0.0004 0 0.0003 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0003 0
Cytoplasm 0.007 0.0008 0.0004 0.0016 0.0004 0.0003 0.0036 0.0003 0.0008 0.0009 0.0002 0.0006 0.0012 0.0001 0.0018 0 0.0015 0.0001
Cytoplasmic Foci 0.0068 0 0.0002 0.0012 0.0048 0.0037 0.0073 0 0.0079 0.0032 0.0011 0.0035 0.0031 0 0.0019 0 0.0052 0.0032
Eisosomes 0.0005 0 0.0027 0 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0034 0 0.0006 0.0001 0.0001 0.0004 0.001 0 0.0003 0.0006 0.0005 0.0001 0.001 0 0.0007 0 0.0006 0
Endosome 0.0142 0 0.0002 0.0004 0.0015 0.0048 0.0173 0 0.0063 0.0172 0.0028 0.0025 0.0027 0 0.0046 0 0.0155 0.0001
Golgi 0.0045 0 0.0002 0.0008 0.0016 0.006 0.0071 0 0.0047 0.0135 0.0027 0.001 0.001 0 0.0003 0 0.0094 0
Lipid Particles 0.0232 0 0.0002 0.0083 0.0052 0.0111 0.0104 0 0.0016 0.0021 0.0058 0.0024 0.0031 0 0.0016 0 0.0074 0.0007
Mitochondria 0.0066 0.0001 0.0039 0.0013 0.0007 0.0156 0.0113 0.0001 0.008 0.0105 0.0286 0.0024 0.0015 0 0.0008 0 0.004 0
None 0.0281 0.0006 0.0009 0.0002 0.0017 0.0002 0.0009 0.0037 0.0001 0.001 0.0002 0.0004 0.0017 0 0.0081 0 0.0109 0
Nuclear Periphery 0.028 0.0026 0.0006 0.0042 0.0169 0.0013 0.0096 0.0012 0.0076 0.0055 0.0052 0.0015 0.0051 0.0007 0.0037 0.0002 0.0029 0.0009
Nucleolus 0.0304 0.0163 0.0137 0.0108 0.0933 0.051 0.0222 0.0174 0.0143 0.0163 0.0554 0.0391 0.0233 0.0132 0.0102 0.008 0.0417 0.028
Nucleus 0.8144 0.9742 0.9637 0.9235 0.7932 0.8754 0.8829 0.9716 0.9268 0.8843 0.8818 0.9361 0.9049 0.9383 0.9473 0.9118 0.811 0.9434
Peroxisomes 0.0043 0 0.0003 0.0325 0.0168 0.0078 0.0042 0 0.0043 0.0009 0.0033 0.0004 0.0019 0 0.0003 0 0.0011 0.0001
Punctate Nuclear 0.0139 0.0051 0.0013 0.0086 0.035 0.0091 0.0078 0.0052 0.0031 0.0153 0.0047 0.0079 0.0404 0.0473 0.0143 0.0797 0.0828 0.0228
Vacuole 0.0022 0.0001 0.0003 0.0006 0.0002 0.0009 0.0052 0.0001 0.0012 0.0012 0.0009 0.0006 0.0015 0 0.0006 0 0.0029 0
Vacuole Periphery 0.0016 0 0.0001 0.0001 0.0001 0.0005 0.0026 0 0.0004 0.0007 0.0015 0.0002 0.0005 0 0.0002 0 0.0008 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0409 6.9799 7.0876 15.1558 9.0579 8.2145 11.0504 14.9166 13.4789 12.8249
Translational Efficiency 1.1373 1.3015 1.236 0.7256 1.0185 1.6129 0.84 0.7954 1.045 0.8515

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2013 1648 879 97 2152 1687 195 215 4165 3335 1074 312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 691.29 814.62 1108.00 930.50 711.24 775.37 1032.61 1128.89 701.60 794.77 1094.31 1067.21
Standard Deviation 77.80 107.48 165.89 137.38 85.92 98.93 137.93 213.42 82.70 105.09 163.77 213.75
Intensity Change Log 2 — 0.236836 0.680595 0.428715 — 0.124548 0.537887 0.666497 — 0.180986 0.609990 0.554185

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.000288 0.000902 0.001214 0.000100 0.000320 0.001291 0.000134 0.000098 0.000304 0.000972 0.000470
Bud Neck 0.002431 0.004853 0.019091 0.006427 0.003522 0.005007 0.007046 0.031516 0.002995 0.004931 0.016904 0.023716
Bud Site 0.000720 0.000844 0.001077 0.000960 0.000588 0.001927 0.000999 0.001189 0.000652 0.001392 0.001063 0.001118
Cell Periphery 0.000111 0.000050 0.000032 0.000056 0.000092 0.000104 0.000048 0.000081 0.000101 0.000077 0.000034 0.000073
Cytoplasm 0.049008 0.008971 0.000156 0.001019 0.019415 0.013949 0.000494 0.004659 0.033718 0.011489 0.000218 0.003527
Cytoplasmic Foci 0.006858 0.000806 0.000015 0.003962 0.002289 0.005014 0.000048 0.000017 0.004497 0.002935 0.000021 0.001243
Eisosomes 0.000018 0.000015 0.000045 0.000040 0.000015 0.000022 0.000049 0.000013 0.000016 0.000019 0.000046 0.000022
Endoplasmic Reticulum 0.000830 0.002287 0.002069 0.005638 0.001278 0.002160 0.006797 0.001506 0.001061 0.002223 0.002928 0.002791
Endosome 0.000549 0.000325 0.000055 0.000977 0.000334 0.001183 0.000163 0.000186 0.000438 0.000759 0.000074 0.000432
Golgi 0.000263 0.000216 0.000092 0.010552 0.000284 0.001204 0.000095 0.000035 0.000274 0.000716 0.000093 0.003305
Lipid Particles 0.001620 0.000359 0.000036 0.000386 0.000669 0.001470 0.000207 0.000010 0.001129 0.000921 0.000067 0.000126
Mitochondria 0.002439 0.001667 0.001301 0.001142 0.001799 0.003133 0.000918 0.000672 0.002108 0.002408 0.001232 0.000818
Mitotic Spindle 0.001792 0.005624 0.000870 0.000108 0.000890 0.006028 0.000201 0.001752 0.001326 0.005829 0.000749 0.001241
None 0.013609 0.005897 0.000874 0.002792 0.009641 0.007767 0.002090 0.002997 0.011559 0.006843 0.001095 0.002933
Nuclear Periphery 0.000624 0.000612 0.000159 0.000370 0.000758 0.001079 0.000262 0.000790 0.000694 0.000848 0.000177 0.000659
Nuclear Periphery Foci 0.000267 0.000531 0.000026 0.000201 0.000327 0.000559 0.000120 0.000032 0.000298 0.000545 0.000043 0.000085
Nucleolus 0.007425 0.012868 0.027609 0.011545 0.011150 0.016070 0.013893 0.004848 0.009350 0.014487 0.025118 0.006930
Nucleus 0.908904* 0.952044* 0.944132* 0.950218* 0.945376* 0.929761* 0.963238* 0.949048* 0.927748* 0.940772* 0.947601* 0.949411*
Peroxisomes 0.001386 0.000533 0.000786 0.000378 0.000466 0.001777 0.000532 0.000017 0.000911 0.001162 0.000740 0.000130
Vacuole 0.000902 0.001017 0.000488 0.001673 0.000842 0.001197 0.001393 0.000347 0.000871 0.001108 0.000652 0.000759
Vacuole Periphery 0.000148 0.000190 0.000184 0.000345 0.000166 0.000267 0.000117 0.000151 0.000157 0.000229 0.000172 0.000211

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.74 -20.16 -6.56 -5.61 -2.47 -3.45 -11.67 -1.95 2.93 11.31 -5.35 -25.30 -6.02 -2.79 5.82
Bud Neck -4.06 -15.77 -5.31 -3.13 7.52 -2.23 -5.81 -6.69 -6.27 -5.06 -4.40 -16.78 -7.25 -6.51 -1.86
Bud Site -0.54 -2.17 -1.57 -1.35 -0.88 -2.29 -4.21 -1.60 1.05 0.06 -2.27 -4.08 -2.15 -0.04 -0.51
Cell Periphery 4.09 5.41 2.07 -1.39 -2.68 -1.03 3.40 0.76 1.51 -1.38 1.90 7.15 1.71 0.38 -2.94
Cytoplasm 14.43 19.68 19.08 6.82 -3.25 2.57 13.32 7.58 4.89 -3.12 12.83 23.53 17.66 6.30 -3.64
Cytoplasmic Foci 6.31 7.44 1.89 -0.66 -1.05 -2.68 5.26 5.32 5.56 1.61 2.51 8.97 4.38 2.52 -1.05
Eisosomes 1.98 -14.30 -4.06 -4.43 -0.54 -3.53 -9.00 1.43 4.90 9.81 -2.01 -17.46 -2.53 -1.60 7.21
Endoplasmic Reticulum -6.79 -7.23 -7.99 -5.87 -6.20 -4.24 -8.83 -0.90 2.57 8.18 -7.74 -11.14 -6.48 -2.40 -0.03
Endosome 1.55 3.81 -0.39 -0.90 -1.50 -3.07 2.64 2.52 3.66 0.40 -1.88 5.20 0.53 1.86 -1.85
Golgi 0.34 2.09 -0.98 -0.98 -1.00 -2.37 1.26 1.88 3.21 6.64 -2.16 2.16 -0.90 -0.69 -0.98
Lipid Particles 3.51 4.55 3.36 -0.03 -3.04 -2.73 4.03 6.01 5.43 4.34 0.87 6.00 5.54 5.61 -1.59
Mitochondria 0.93 1.47 1.62 1.23 0.74 -1.92 1.53 2.20 4.06 2.49 -0.79 1.77 2.87 4.47 4.31
Mitotic Spindle -2.47 0.80 1.96 3.66 0.96 -3.72 1.28 -0.92 1.96 -1.27 -4.33 0.60 -0.31 3.06 -0.64
None 5.28 10.45 8.52 3.66 -3.27 1.49 6.92 5.33 3.84 -0.85 4.83 13.44 9.08 4.73 -2.74
Nuclear Periphery 0.02 6.61 1.90 1.68 -3.95 -0.94 5.70 -0.88 -0.08 -1.85 -0.89 10.10 -0.77 -0.18 -2.41
Nuclear Periphery Foci -0.66 4.06 0.35 0.78 -1.98 -0.94 1.60 2.19 2.87 1.91 -1.09 3.55 2.48 2.39 -1.60
Nucleolus -4.10 -10.93 -1.55 0.83 7.03 -3.86 -1.27 4.83 7.24 3.60 -5.59 -10.52 2.43 6.32 10.88
Nucleus -8.14 -6.29 -3.74 -0.01 -1.13 3.25 -3.40 -0.51 -2.60 1.49 -3.39 -4.54 -3.65 -1.60 -1.21
Peroxisomes 1.75 1.27 1.97 0.42 2.44 -2.63 -0.12 3.31 3.71 7.74 -0.72 0.64 3.28 3.99 9.49
Vacuole -0.75 2.28 -2.44 -1.48 -3.41 -0.24 -1.16 2.04 3.06 5.75 -0.66 1.38 0.57 1.25 -0.81
Vacuole Periphery -0.85 -0.75 -0.93 -0.58 -0.58 -1.46 0.89 0.36 1.62 -0.25 -1.66 -0.56 -0.49 0.70 -0.03
Standard name
Human Ortholog
Description Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Gis1

Gis1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gis1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available