Standard name
Description Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.14 0.11 0.06 0.1 0.09 0.08 0.08 0.05 0 0.15 0.17 0.19 0.21 0.21 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0.44 0.47 0.2 0.47 0.41 0.48 0.59 0.62 0.59 0.65 0.09 0 0.15 0.09 0 0.08 0 0 0 0 0 0
Nucleus 0.94 0.86 0.81 0.74 0.79 0.77 0.77 0.76 0.73 0.73 0.74 0.74 0.76 0.76 0.69 0.7 0.69 0.72 0.88 0.81 0.79 0.83 0.75 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0.06 0.06 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0.06 0.09 0.12 0.09 0.15 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 3 2 3 8 4 5 3 0 0 0 0 0 0 2 2 3 1 4 2
Bud Neck 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 1 1 2 7 14 22 17 17 25 26 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 18 5 8 21 20 20 40 53 32 36 19 14 30 39 43 43 31 28 5 2 4 3 4 7
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 7 7 3 0 0 0 0 0 0
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 2 2 3 4 3 3 1 1 0 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 1 0 0 0 0 0
Mitochondria 2 30 91 72 38 146 165 285 242 273 218 222 18 8 35 18 1 13 4 3 4 1 1 6
Nucleus 385 104 169 114 147 242 308 449 303 318 272 253 150 172 156 144 102 117 374 106 173 129 172 117
Nuclear Periphery 1 0 0 0 1 1 5 3 6 9 7 2 0 1 1 2 0 0 1 0 0 0 0 0
Nucleolus 0 1 0 1 1 6 9 30 23 28 29 35 3 4 1 2 2 3 0 0 0 1 2 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 2 1 1 2 1 1 0 1 0 1 1 0 0 5 1 4 2 1 5
Vac/Vac Membrane 6 6 2 3 7 4 7 8 4 2 5 3 5 10 7 13 8 6 24 12 26 13 35 60
Unique Cell Count 409 121 209 154 187 313 402 588 413 438 370 340 197 225 226 207 147 162 425 132 221 157 230 214
Labelled Cell Count 414 146 272 212 218 432 551 855 638 688 581 558 209 236 249 236 155 175 425 132 221 157 230 214


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.3 3.9 4.2 4.8 4.2 4.3 4.2 3.7 3.7 3.6 3.5 5.4 5.4 4.7 8.7 9.7 9.2 5.1 5.1 5.6
Std Deviation (1e-4) 0.9 1.0 0.7 1.5 1.7 1.3 1.9 1.7 1.4 1.4 1.1 1.3 1.5 1.6 1.5 2.8 3.4 2.9 1.4 1.6 1.8
Intensity Change (Log2) 0.09 0.31 0.09 0.15 0.11 -0.06 -0.09 -0.11 -0.16 0.47 0.47 0.26 1.16 1.31 1.24 0.4 0.39 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 2.9