Standard name
Human Ortholog
Description Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.18 0.15
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.19 0.18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.96 0.89 0.96 0.93 0.91 0.86 0.81 0.81 0.72 0.75 0.94 0.93 0.88 0.87 0.82 0.81 0.77 0.78 0.86 0.41 0.36 0.48
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.05 0.11 0.06 0.18 0.21 0.27 0.38 0.4 0.52 0.47 0 0 0.06 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.11 0.19 0.22 0.21 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 14 35 48
Bud 0 5 3 3 3 5 7 15 14 9 13 2 0 1 0 0 0 0 0 0 10 36 56
Bud Neck 0 0 0 0 0 1 3 0 1 2 1 0 0 0 0 0 0 0 0 0 8 23 27
Bud Site 0 0 0 1 0 2 1 1 2 1 1 0 0 0 0 0 0
Cell Periphery 0 0 0 2 4 3 3 1 2 2 5 1 2 0 1 0 3 0 0 0 0 3 3
Cytoplasm 47 144 300 626 499 825 886 586 670 334 567 136 268 290 286 168 216 56 22 142 30 69 152
Endoplasmic Reticulum 0 1 2 1 2 0 3 0 1 1 0 0 1 2 16 10 8 2 0 1 0 0 2
Endosome 0 0 0 3 0 1 1 0 0 0 0 1 1 1 4 4 4 2 0 1 3 5 5
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 2 3 0 0 0 0 0
Mitochondria 7 8 37 41 98 193 274 277 333 241 355 1 0 21 10 1 5 2 0 4 1 5 5
Nucleus 0 0 6 2 1 3 3 8 3 2 4 2 3 4 11 9 6 0 3 0 0 1 0
Nuclear Periphery 0 0 0 0 0 1 0 1 2 6 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 2 0 1 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 1 0 0 1 2 2 2 1 0 0 0 0 0 1 2 0 1 1 2 4
Vac/Vac Membrane 1 2 14 14 10 11 21 13 16 3 18 11 20 37 61 45 55 0 1 5 1 6 8
Unique Cell Count 54 150 336 655 535 908 1027 722 825 467 760 145 289 330 329 205 267 74 29 165 74 193 320
Labelled Cell Count 56 161 363 694 620 1045 1204 904 1048 603 965 154 296 356 391 238 300 74 29 165 74 193 320


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.0 4.0 4.0 3.7 3.3 3.5 3.0 3.0 3.0 2.8 5.8 5.8 4.9 6.0 6.6 7.3 5.7 5.5 6.1
Std Deviation (1e-4) 0.7 1.2 0.5 0.7 0.9 0.7 0.8 0.8 0.7 0.8 0.6 1.1 1.1 0.9 1.4 1.5 1.7 1.6 1.3 1.2
Intensity Change (Log2) -0.01 0.01 -0.13 -0.27 -0.2 -0.42 -0.42 -0.4 -0.52 0.53 0.53 0.3 0.6 0.72 0.88 0.52 0.46 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.4 -0.2 -1.2 -2.1 -3.4 -4.5 -4.5 -6.2 -5.8 -0.9 -1.4 -2.8 -3.0 -4.0 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.4 -1.2 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 2.6 2.5 3.7 5.2 5.7 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7362 2.1789 1.9313 1.3635 1.5263 1.9685 2.9354 3.2269 3.0316 2.8847 2.776 2.848 0.4322 1.0616 0.9123 1.2357 0.4961 0.7833
Actin 0.0355 0.002 0.0128 0.0238 0.0258 0.0116 0.048 0.002 0.0403 0.0007 0.0105 0.0025 0.019 0.0013 0.0147 0.0042 0.0074 0.0019
Bud 0.0009 0.0008 0.0017 0.0014 0.0007 0.0007 0.0052 0.0043 0.0018 0.0002 0.0007 0.0017 0.0009 0.0005 0.0001 0.0045 0.0059 0.0034
Bud Neck 0.0095 0.001 0.0091 0.002 0.0017 0.0093 0.0093 0.0005 0.002 0.0006 0.0011 0.0039 0.0083 0.0006 0.0002 0.0018 0.0004 0.0023
Bud Periphery 0.0015 0.0006 0.0018 0.002 0.0023 0.0009 0.0069 0.0028 0.0018 0.0001 0.0007 0.0022 0.0014 0.0003 0.0001 0.0049 0.0097 0.0067
Bud Site 0.0082 0.0097 0.0155 0.0016 0.0008 0.0005 0.0145 0.0055 0.0048 0.0002 0.0004 0.0002 0.0086 0.036 0.0003 0.0472 0.0047 0.0007
Cell Periphery 0.0006 0.0003 0.0004 0.0003 0.0003 0.0001 0.0007 0.0001 0.0002 0 0.0001 0 0.0006 0.0009 0.0001 0.0007 0.0002 0.0003
Cytoplasm 0.7254 0.7795 0.7468 0.7992 0.7835 0.8131 0.5552 0.8081 0.7692 0.79 0.651 0.7997 0.6123 0.6863 0.613 0.6273 0.6177 0.6806
Cytoplasmic Foci 0.0144 0.0105 0.0144 0.0095 0.0104 0.0099 0.0226 0.0092 0.0106 0.0074 0.0176 0.0082 0.0105 0.0029 0.0089 0.0143 0.0091 0.0029
Eisosomes 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0008 0 0.0008 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0117 0.0077 0.0074 0.0125 0.0098 0.0079 0.0054 0.0032 0.002 0.0065 0.0089 0.0035 0.0031 0.0032 0.003 0.0083 0.0028 0.0027
Endosome 0.0197 0.0045 0.008 0.0049 0.0146 0.0065 0.0128 0.002 0.0117 0.021 0.0196 0.0072 0.0122 0.0008 0.0022 0.0025 0.0046 0.0022
Golgi 0.0084 0.0004 0.0022 0.0031 0.0037 0.0008 0.0059 0.0006 0.0048 0.002 0.0124 0.0022 0.0032 0.0001 0.0017 0.0028 0.0033 0.0002
Lipid Particles 0.0038 0.0009 0.0042 0.0034 0.0046 0.0008 0.0239 0.0001 0.0006 0.0053 0.0055 0.0021 0.0025 0.0002 0.0043 0.0041 0.026 0.0001
Mitochondria 0.0057 0.0003 0.0072 0.0104 0.0105 0.0009 0.0157 0.0011 0.0055 0.0007 0.0215 0.0151 0.0012 0.0001 0.0007 0.0025 0.0097 0.0003
None 0.084 0.145 0.1305 0.083 0.0518 0.0616 0.1859 0.1153 0.1323 0.1419 0.2112 0.0409 0.2886 0.262 0.3389 0.213 0.2763 0.2854
Nuclear Periphery 0.0107 0.0025 0.0055 0.0038 0.0089 0.0068 0.0234 0.0022 0.0009 0.0094 0.0039 0.0028 0.0023 0.0009 0.0007 0.0009 0.0017 0.001
Nucleolus 0.0008 0.0004 0.0017 0.0002 0.0006 0.0006 0.0006 0.0002 0.0001 0.0002 0.0002 0.0001 0.0021 0.0001 0.0001 0.0014 0.0004 0.0001
Nucleus 0.0447 0.0278 0.0147 0.0304 0.0515 0.0565 0.0149 0.0359 0.0054 0.0076 0.0142 0.102 0.0124 0.0022 0.0026 0.0024 0.0043 0.0073
Peroxisomes 0.0032 0.0004 0.0071 0.0042 0.0014 0.0006 0.0316 0.0025 0.0029 0.0004 0.0151 0.0006 0.0018 0 0.007 0.0518 0.0126 0.0001
Punctate Nuclear 0.0066 0.003 0.005 0.0021 0.0055 0.0079 0.0128 0.0035 0.0012 0.0032 0.0037 0.0031 0.003 0.0007 0.0007 0.0007 0.0014 0.0006
Vacuole 0.0035 0.0025 0.0034 0.0016 0.0068 0.0026 0.0021 0.0007 0.0009 0.0019 0.0009 0.0015 0.0052 0.0008 0.0006 0.0042 0.0012 0.0011
Vacuole Periphery 0.001 0.0002 0.0006 0.0003 0.0046 0.0004 0.0019 0.0001 0.0003 0.0008 0.0007 0.0005 0.0005 0.0001 0.0001 0.0002 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4236 10.7448 13.5543 18.9335 13.7659 9.21 19.5554 18.2091 15.2463 20.9174
Translational Efficiency 0.6005 0.8947 0.9019 0.6262 0.7544 1.2822 0.5924 0.6538 0.8665 0.6744

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency