Standard name
Human Ortholog
Description Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.07 0 0.06 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.17 0.06 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.07 0.08 0 0 0.05 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.16 0.14 0.19 0.07 0.05 0.07 0.07 0.06 0.05 0.08 0.06 0.16 0 0.06 0.06 0.07 0 0.05 0.06 0.07 0 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.81 0.77 0.7 0.62 0.55 0.51 0.56 0.53 0.49 0.59 0.58 0.74 0.55 0.62 0.77 0.78 0.78 0.42 0.43 0.44 0.38 0.31 0.29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.4 0.48 0.73 0.76 0.81 0.83 0.84 0.8 0.84 0.7 0.8 0.58 0.7 0.62 0.6 0.56 0.59 0.43 0.36 0.37 0.51 0.49 0.49
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0
Bud 1 0 0 0 1 1 5 2 7 5 5 0 0 0 0 1 1 2 1 0 0 0 1
Bud Neck 13 1 3 10 18 10 28 14 18 21 18 4 1 3 1 3 1 3 4 1 0 0 2
Bud Site 8 0 0 1 2 5 4 9 8 16 14 0 0 0 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 2 9 7 5 9 6 6 6 6 4 25 23 7 9 14 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 2 0 0 0 0 0 0
Endosome 6 9 5 4 9 7 14 4 6 2 6 3 8 6 8 14 13 12 8 7 0 4 7
Golgi 0 0 1 0 1 0 0 0 1 0 0 0 0 1 1 0 0 0 0 1 0 0 0
Mitochondria 26 2 0 0 0 6 1 12 5 22 13 1 0 0 6 8 4 0 1 1 0 1 1
Nucleus 48 24 24 18 14 21 34 14 17 24 23 17 7 8 7 14 2 17 12 10 0 5 2
Nuclear Periphery 1 1 1 0 0 0 2 0 0 0 0 1 0 1 1 2 2 2 2 0 0 0 0
Nucleolus 250 137 87 151 149 157 275 125 169 183 238 78 88 84 84 155 124 139 83 67 12 28 35
Peroxisomes 3 3 0 0 1 3 5 2 1 8 2 0 0 0 1 4 2 0 1 0 0 0 0
SpindlePole 122 85 90 185 220 257 412 188 289 217 332 61 111 85 65 112 93 140 70 56 16 45 59
Vac/Vac Membrane 0 2 0 1 1 1 0 0 1 0 0 0 1 1 0 2 3 3 0 1 0 2 2
Unique Cell Count 307 177 124 242 270 308 490 236 344 312 413 106 159 136 109 199 158 330 194 153 33 94 122
Labelled Cell Count 481 265 213 379 423 473 789 376 528 504 657 169 242 213 182 325 262 330 194 153 33 94 122


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.1 8.0 7.2 7.4 5.8 6.0 5.0 5.4 4.7 5.0 7.5 7.6 7.2 10.9 10.2 10.2 7.0 7.9
Std Deviation (1e-4) 1.3 1.5 1.3 1.5 1.7 1.3 1.3 1.4 1.2 1.0 1.1 1.4 1.8 1.7 3.2 2.6 2.4 1.7 1.5
Intensity Change (Log2) -0.15 -0.12 -0.46 -0.41 -0.69 -0.57 -0.78 -0.68 -0.09 -0.08 -0.16 0.45 0.35 0.34 -0.19 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 1.7 0 1.5 1.5 1.3 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 4.0 4.2 0 0 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0.4 0.2 1.1 1.1 1.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -3.4 -4.4 -3.9 -4.2 -3.9 -4.9 -3.5 -4.8 -0.7 -4.0 -3.3 -2.9 -3.3 -5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -2.8 -3.6 -2.8 -3.2 -4.0 0 -2.5 0.6 -2.5 -1.4 1.2 1.6 1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.8 2.0 2.6 3.0 1.5 2.8 0 1.9 -2.4 -0.5 -1.7 -2.1 -2.9 -2.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1027 1.8492 1.8997 2.0291 1.5653 1.69 2.766 2.6522 2.388 1.6385 1.8621 2.4075 1.0755 0.4914 0.2469 0.3315 0.1879 0.3195
Actin 0.0143 0 0.0181 0.0117 0.0002 0.0047 0.0217 0 0.0089 0.0036 0 0.0009 0.0494 0.0001 0.0015 0.0028 0.1098 0.0004
Bud 0.0002 0.0006 0.0003 0.0016 0 0.0002 0.0003 0 0.0009 0.0013 0 0 0.0008 0.0001 0.0001 0.0003 0.0004 0.0007
Bud Neck 0.0009 0.0035 0.0012 0.0011 0.0006 0.0009 0.0018 0.0017 0.0014 0.0036 0.001 0.0009 0.0021 0.0013 0.0023 0.0197 0.005 0.0008
Bud Periphery 0.0002 0.0006 0.0004 0.0029 0.0001 0.0003 0.0004 0.0001 0.002 0.0021 0.0001 0 0.0013 0.0002 0.0001 0.0016 0.0006 0.0003
Bud Site 0.0025 0.0027 0.0015 0.0024 0.0001 0.0004 0.0028 0.0009 0.0098 0.0224 0.0002 0.0002 0.0024 0.0013 0.0011 0.0018 0.0014 0.0003
Cell Periphery 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0003 0.0002 0.0004 0.0009 0.0001 0 0.0003 0.0002 0.0003 0.0029 0.0003 0.0001
Cytoplasm 0.005 0.0001 0.0066 0.0004 0.0001 0.0002 0.0051 0.0001 0.0052 0.0005 0.0001 0.0001 0.002 0 0.013 0.0007 0.0006 0.0049
Cytoplasmic Foci 0.0526 0.0082 0.0212 0.0505 0.0174 0.0201 0.0358 0.0168 0.0199 0.034 0.0142 0.0143 0.0389 0.0104 0.0143 0.0102 0.0191 0.0149
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0002 0 0.0001 0.0002 0 0 0.0002 0 0 0.0025 0.0003 0
Endoplasmic Reticulum 0.0008 0 0.0015 0.0017 0.0002 0.0001 0.0019 0 0.0056 0.0006 0 0.0001 0.003 0 0.0009 0.001 0.0004 0.0001
Endosome 0.0072 0.0004 0.0057 0.014 0.0031 0.0077 0.0113 0.0008 0.0085 0.024 0.0009 0.0016 0.0384 0.0004 0.0017 0.0054 0.0149 0.0022
Golgi 0.0056 0.0006 0.0041 0.0109 0.0055 0.0097 0.0064 0.0007 0.002 0.0081 0.0009 0.0018 0.0187 0.0005 0.0018 0.0023 0.0177 0.0016
Lipid Particles 0.0995 0.0262 0.0488 0.1485 0.2171 0.0529 0.0671 0.0297 0.0361 0.1175 0.0363 0.035 0.0621 0.0149 0.0401 0.0343 0.1853 0.0317
Mitochondria 0.0033 0.0001 0.0027 0.0244 0.0015 0.0052 0.0028 0.0001 0.0096 0.0122 0.0002 0.0005 0.0092 0.0045 0.0006 0.0037 0.0199 0.0027
None 0.0084 0.0001 0.0046 0.0006 0.0001 0 0.0103 0 0.0021 0.0007 0.0001 0.0001 0.0016 0.0001 0.0012 0.0077 0.0007 0.0005
Nuclear Periphery 0.0021 0.0013 0.0016 0.016 0.0048 0.0003 0.005 0.001 0.004 0.0031 0.0009 0.0003 0.0038 0.0012 0.0004 0.0015 0.0009 0.0017
Nucleolus 0.4943 0.672 0.7009 0.519 0.344 0.4029 0.5777 0.6207 0.6952 0.374 0.4874 0.3796 0.5342 0.6728 0.6182 0.6923 0.2614 0.3042
Nucleus 0.0239 0.0418 0.032 0.0293 0.0205 0.0192 0.0294 0.0517 0.0297 0.017 0.0237 0.0177 0.0397 0.0597 0.0493 0.0651 0.0319 0.0222
Peroxisomes 0.007 0.0005 0.0032 0.0037 0.0039 0.0019 0.0093 0.0009 0.002 0.0062 0.0016 0.0058 0.0064 0.0006 0.0068 0.0026 0.0262 0.0048
Punctate Nuclear 0.2708 0.2405 0.1446 0.1564 0.3793 0.472 0.2082 0.2735 0.1548 0.3586 0.4321 0.5409 0.1809 0.2303 0.2452 0.1351 0.3007 0.6054
Vacuole 0.0007 0.0006 0.0005 0.0024 0.0004 0.0008 0.0016 0.0009 0.0014 0.0057 0.0002 0.0001 0.003 0.0007 0.0012 0.0055 0.0013 0.0003
Vacuole Periphery 0.0006 0.0001 0.0002 0.0021 0.0011 0.0005 0.0006 0.0001 0.0005 0.0036 0.0001 0 0.0015 0.0004 0.0002 0.0011 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9196 9.858 12.9089 17.9497 14.7182 10.3731 13.0455 11.2781 15.1825 19.7611
Translational Efficiency 1.1941 1.0092 1.0113 0.7481 0.8988 1.2697 0.7032 0.8534 0.6948 0.8537

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
917 728 1767 607 2100 1990 1117 2540 3017 2718 2884 3147

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 595.92 855.14 986.90 1013.90 827.35 851.41 973.46 1132.00 757.01 852.41 981.69 1109.22
Standard Deviation 134.20 200.91 135.63 160.66 124.73 138.16 153.66 186.07 166.24 157.45 143.04 187.33
Intensity Change Log 2 0.521042 0.727785 0.766725 0.041356 0.234624 0.452304 0.261873 0.461909 0.592373

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000296 0.000525 0.000248 0.000455 0.000254 0.000352 0.000435 0.000402 0.000266 0.000398 0.000321 0.000412
Bud Neck 0.007463 0.008764 0.005568 0.020189 0.004665 0.007668 0.008771 0.018963 0.005516 0.007961 0.006809 0.019199
Bud Site 0.002265 0.003632 0.001617 0.008836 0.001552 0.001769 0.003067 0.004596 0.001769 0.002268 0.002178 0.005414
Cell Periphery 0.000258 0.000135 0.000032 0.000218 0.000108 0.000089 0.000150 0.000084 0.000154 0.000101 0.000078 0.000110
Cytoplasm 0.013656 0.008683 0.000265 0.002031 0.002497 0.001176 0.011081 0.002624 0.005889 0.003187 0.004454 0.002510
Cytoplasmic Foci 0.019316 0.008097 0.001039 0.006983 0.004389 0.004070 0.004768 0.001535 0.008926 0.005149 0.002483 0.002586
Eisosomes 0.000184 0.000136 0.000062 0.000085 0.000263 0.000183 0.000083 0.000077 0.000239 0.000170 0.000070 0.000078
Endoplasmic Reticulum 0.003190 0.003686 0.000807 0.001032 0.002872 0.003086 0.001757 0.000928 0.002968 0.003247 0.001175 0.000948
Endosome 0.007258 0.007515 0.000577 0.005481 0.002402 0.003752 0.001714 0.002151 0.003878 0.004760 0.001018 0.002793
Golgi 0.006889 0.006230 0.001096 0.002485 0.003068 0.002720 0.001768 0.001344 0.004229 0.003660 0.001356 0.001564
Lipid Particles 0.006986 0.002303 0.000166 0.000701 0.001808 0.002854 0.001284 0.000190 0.003382 0.002706 0.000599 0.000289
Mitochondria 0.002685 0.002412 0.000708 0.002261 0.001187 0.002762 0.000923 0.003411 0.001643 0.002668 0.000791 0.003189
Mitotic Spindle 0.130050* 0.225761* 0.291175* 0.348152* 0.183132* 0.194368* 0.285565* 0.214412* 0.166998* 0.202776* 0.289002* 0.240208*
None 0.024784 0.005006 0.000295 0.001325 0.002025 0.000807 0.010076 0.001101 0.008943 0.001932 0.004084 0.001144
Nuclear Periphery 0.001165 0.001702 0.000804 0.001997 0.001100 0.001727 0.000925 0.001531 0.001120 0.001720 0.000851 0.001621
Nuclear Periphery Foci 0.136323 0.074176 0.007740 0.007460 0.088482 0.060904 0.024047 0.002978 0.103023 0.064459 0.014056 0.003842
Nucleolus 0.516410* 0.443606* 0.543147* 0.430499* 0.552389* 0.524279* 0.441659* 0.549864* 0.541453* 0.502671* 0.503839* 0.526840*
Nucleus 0.101755 0.174988* 0.129722 0.130560* 0.124602 0.167634* 0.188165* 0.184408* 0.117657 0.169604* 0.152357* 0.174021*
Peroxisomes 0.017223 0.018139 0.013599 0.016181 0.021972 0.017630 0.011185 0.006045 0.020529 0.017766 0.012664 0.008000
Vacuole 0.001252 0.003228 0.000941 0.008526 0.000894 0.001505 0.002117 0.001768 0.001003 0.001966 0.001396 0.003072
Vacuole Periphery 0.000591 0.001276 0.000393 0.004541 0.000338 0.000665 0.000460 0.001588 0.000415 0.000829 0.000419 0.002158

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.31 -0.44 -6.54 -0.82 -6.55 -3.95 -7.22 -1.27 -0.33 1.20 -6.73 -5.78 -1.97 -0.27 -0.62
Bud Neck -3.03 -1.11 -11.51 -9.72 -11.40 -6.90 -9.24 -20.04 -15.68 -10.63 -6.92 -7.41 -22.57 -18.17 -17.36
Bud Site -3.24 -0.28 -7.31 -5.90 -7.29 -0.68 -7.27 -8.71 -8.84 -0.31 -2.58 -5.93 -11.28 -9.98 -7.41
Cell Periphery 3.23 8.36 0.22 -1.38 -3.49 1.68 -1.39 2.20 0.40 3.37 3.45 5.65 2.50 -0.51 -2.60
Cytoplasm 0.59 5.11 3.25 2.10 -3.98 1.82 -3.88 -1.44 -3.62 3.22 1.71 1.29 1.52 -0.48 0.10
Cytoplasmic Foci 5.49 8.62 4.88 -0.61 -4.74 0.94 -0.06 5.34 4.30 4.69 5.41 8.35 7.81 3.05 -0.77
Eisosomes 7.67 23.35 11.28 5.51 -2.39 10.13 24.75 27.80 22.63 5.12 11.90 33.41 31.10 22.74 0.20
Endoplasmic Reticulum -2.96 15.13 9.33 10.31 -2.95 -1.36 7.86 18.21 15.34 8.59 -2.64 19.57 20.74 18.69 3.90
Endosome -0.16 7.52 1.69 1.43 -3.57 -2.93 1.00 1.05 3.90 0.09 -1.62 7.68 2.97 3.93 -4.34
Golgi 2.08 13.00 8.26 3.80 -2.67 2.31 5.30 11.10 8.29 6.25 3.38 15.50 14.00 7.43 0.25
Lipid Particles 5.96 8.80 8.06 4.80 -4.70 -1.69 1.70 6.53 4.70 2.43 1.68 8.90 10.44 5.69 1.62
Mitochondria -0.13 5.37 -0.38 -0.17 -7.05 -2.43 1.34 -5.16 -1.00 -6.25 -2.30 4.31 -4.82 -1.12 -7.34
Mitotic Spindle -6.14 -13.84 -11.45 -5.66 -2.21 -0.82 -9.90 -3.50 -2.65 7.49 -4.08 -15.88 -8.91 -4.65 7.11
None 5.94 8.06 7.63 4.30 -4.86 3.53 -3.84 1.91 -4.48 4.48 6.78 5.16 7.73 2.49 3.12
Nuclear Periphery -4.17 3.34 -5.24 -1.71 -6.42 -4.65 1.05 -3.63 1.19 -4.06 -5.89 3.77 -5.37 0.60 -6.99
Nuclear Periphery Foci 8.15 19.99 20.08* 11.84 1.32 6.24 15.48 25.09* 20.53* 10.66 10.15 27.26 31.99 23.73* 11.74
Nucleolus 4.50 -2.52 5.66 1.72 8.43 2.77 10.36 0.70 -2.08 -9.87 4.50 4.92 2.44 -1.94 -2.43
Nucleus -11.85 -9.16 -6.34 3.86 -0.85 -10.27 -6.29 -14.18 -3.78 -2.90 -15.32 -8.47 -16.70 -1.76 -7.53
Peroxisomes 0.51 4.32 0.66 0.11 -3.85 4.26 8.20 20.33 17.08 10.08 3.72 9.85 18.00 14.46 10.95
Vacuole -2.71 -1.11 -4.92 -3.65 -4.78 -3.96 -5.72 -5.05 -1.96 2.23 -4.18 -5.21 -6.41 -3.70 -3.98
Vacuole Periphery -1.76 0.26 -3.29 -2.29 -3.32 -5.48 -4.95 -9.53 -6.42 -8.09 -3.52 -2.51 -7.30 -4.59 -6.90