Standard name
Description Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.51 0.47 0.44 0.53 0.47 0.4 0.35 0.32 0.42 0.37 0.44 0.38 0.5 0.41 0.37 0.37 0.46 0.33 0.82 0.75 0.78 0.71 0.74 0.75
Bud 0.11 0.13 0.14 0.19 0.18 0.11 0.11 0.14 0.12 0.13 0.15 0.12 0.12 0.08 0.07 0 0.07 0.08 0.06 0.09 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0.07 0.07 0.06 0 0 0 0 0 0 0 0 0.07 0.11 0.09 0.07 0 0.07 0 0 0 0 0 0
Cytoplasm 0.12 0.12 0.06 0.07 0.06 0 0 0 0 0 0 0 0.07 0.09 0.05 0.06 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.12 0 0 0.06 0.05 0 0.05 0 0 0 0 0.1 0.05 0.05 0.06 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.44 0.42 0.48 0.58 0.58 0.36 0.44 0.49 0.61 0.39 0.5 0.47 0.61 0.76 0.79 0.76 0.72 0.65 0.05 0.07 0.07 0.1 0.11 0.1
Mitochondria 0.16 0.09 0.22 0.09 0.07 0.47 0.37 0.25 0.33 0.38 0.42 0.43 0 0 0 0.17 0.15 0.09 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.09 0.09 0 0.08 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 41 97 132 115 127 175 186 168 184 187 180 165 108 175 183 52 53 39 90 222 324 280 501 474
Bud 9 28 43 42 48 50 60 73 54 64 62 54 27 33 34 6 8 10 6 26 13 15 15 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 2 3 2
Bud Site 1 0 3 2 0 2 3 3 1 3 2 2 1 0 0 0 0 0 – – – – – –
Cell Periphery 4 15 21 14 9 15 21 22 12 19 15 7 15 47 45 10 5 8 0 0 0 0 0 2
Cytoplasm 10 24 17 15 16 13 11 15 9 15 6 8 15 39 25 8 5 9 3 3 4 1 4 1
Endoplasmic Reticulum 5 25 12 4 16 20 14 28 11 18 10 15 22 23 23 9 7 9 0 2 1 2 4 9
Endosome 0 0 3 0 0 0 0 0 0 1 0 1 0 0 1 0 0 1 1 6 5 13 18 16
Golgi 35 88 145 126 158 157 234 256 265 198 206 202 132 327 397 108 82 77 5 21 28 39 71 60
Mitochondria 13 19 65 19 19 209 196 132 143 193 174 186 6 0 1 24 17 11 0 1 1 4 9 6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 3 2 11 4 11 20 45 46 14 41 17 24 0 4 3 2 2 2 0 0 1 0 0 0
Peroxisomes 0 1 11 1 0 2 4 0 1 6 2 3 1 0 3 1 0 0 0 1 27 25 33 38
SpindlePole 0 1 4 3 4 2 4 7 1 3 1 1 1 8 3 0 0 2 0 0 2 0 3 3
Vac/Vac Membrane 0 1 3 1 3 0 3 0 1 0 0 1 0 4 4 1 1 3 0 3 0 1 3 0
Unique Cell Count 80 208 299 217 273 440 529 526 438 505 411 432 217 430 500 142 114 118 111 295 415 394 673 637
Labelled Cell Count 121 301 471 346 411 665 781 750 696 748 675 669 328 661 722 221 181 172 111 295 415 394 673 637


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 39.7 39.0 34.0 34.7 35.9 35.9 37.2 39.3 40.9 39.7 38.8 38.1 38.6 44.7 46.0 51.6 52.3 51.6 33.7 34.7 40.4
Std Deviation (1e-4) 10.2 12.7 10.8 11.4 12.3 10.8 11.5 12.6 14.2 13.7 13.7 13.4 11.8 13.5 15.7 16.8 19.9 20.2 11.4 9.4 12.6
Intensity Change (Log2) – – – 0.03 0.08 0.08 0.13 0.21 0.27 0.22 0.19 0.16 0.18 0.39 0.44 0.6 0.62 0.6 -0.01 0.03 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.0 0.6 -1.2 -2.6 -3.5 -0.6 -2.0 -0.1 -1.6 1.3 -0.9 -2.1 -1.5 0.4 -2.1
Bud 1.5 1.0 -1.2 -1.3 -0.2 -0.8 -0.7 0.3 -0.7 -0.6 -2.9 -3.5 -3.2 -2.0 -1.6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.3 -2.0 -2.2 -1.9 -1.8 -2.8 -2.1 -2.0 -3.7 0 1.8 1.0 0 -1.0 -0.1
Cytoplasm 0.6 0.1 -1.8 -2.8 -2.0 -2.6 -1.9 -3.1 -2.8 0.6 1.7 -0.4 0 -0.5 0.7
Endoplasmic Reticulum -1.4 1.0 0.3 -1.1 0.8 -1.2 -0.3 -1.2 -0.4 2.8 0.8 0.4 1.1 0.9 1.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.1 2.2 -3.5 -1.2 0 3.2 -2.6 0.4 -0.5 2.8 7.7 9.1 5.5 4.3 3.1
Mitochondria -3.9 -5.0 7.1 4.6 1.1 3.2 4.8 5.7 6.0 -6.2 -10.1 -10.7 -1.2 -1.6 -3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.2 0.2 0.6 2.7 2.8 -0.4 2.5 0.3 1.2 0 -2.6 -3.2 0 0 0
Peroxisomes -2.4 -3.2 -3.3 -3.0 0 0 -2.4 -3.1 -2.9 -2.4 0 -3.2 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0