ORF
Description F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.95 0.95 0.96 0.95 0.93 0.92 0.91 0.84 0.85 0.76 0.81 0.98 0.97 0.95 0.84 0.86 0.85 0.67 0.6 0.54 0.65 0.72 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0 0 0 0.16 0.12 0.34 0.16 0 0 0 0 0 0 0.11 0.07 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0.05 0 0 0.07 0.07 0 0.06 0 0 0.07 0.18 0.18 0.17 0.1 0.27 0.29 0.22 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 1 0 1 3
Bud 1 0 0 1 0 1 1 5 3 4 10 2 1 1 0 0 0 0 0 0 0 0 2 6
Bud Neck 0 1 2 5 11 3 10 20 11 19 5 7 0 0 1 2 3 0 2 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 6 1 1 4 1 3 2 0 0 0 0 1 5 9 7 0 0 0 0 0 0
Cytoplasm 146 281 53 225 330 340 538 658 319 368 334 433 158 377 451 251 341 334 117 182 47 51 129 184
Endoplasmic Reticulum 3 3 0 1 0 0 3 0 0 1 2 1 0 3 0 17 27 32 2 1 0 1 0 1
Endosome 0 0 0 0 0 0 3 4 4 2 0 4 0 4 3 3 3 4 8 3 1 0 1 3
Golgi 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 4 1 0 1 0 0 0 1
Mitochondria 19 5 0 2 0 13 25 24 61 54 149 86 1 0 3 1 6 2 18 21 6 3 5 9
Nucleus 0 0 0 1 0 4 4 6 3 4 4 1 0 5 2 2 4 4 0 2 0 1 0 0
Nuclear Periphery 1 0 0 1 0 1 0 1 0 0 0 2 0 0 0 1 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 1 0 0 1 5 5 11 15 5 12 6 19 0 0 1 0 1 1 1 2 1 0 1 1
Vac/Vac Membrane 3 9 3 4 9 18 24 20 25 31 10 32 3 7 34 53 71 66 17 82 25 17 29 51
Unique Cell Count 161 295 56 235 346 367 584 721 381 434 439 536 161 388 475 298 396 391 176 305 88 79 179 272
Labelled Cell Count 175 300 58 248 356 386 623 757 434 499 522 589 163 397 496 336 471 452 176 305 88 79 179 272


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 5.3 4.8 5.2 4.4 4.5 4.8 4.0 4.0 3.8 4.0 5.3 5.2 5.2 5.9 5.7 5.6 5.2 5.4 5.4
Std Deviation (1e-4) 0.7 0.7 0.8 1.0 1.3 1.2 1.3 1.5 1.4 1.1 1.4 1.1 1.4 1.3 1.7 1.3 1.4 1.5 1.9 1.9 1.8
Intensity Change (Log2) – – – -0.12 -0.01 -0.25 -0.23 -0.13 -0.39 -0.39 -0.48 -0.4 0.0 -0.01 -0.03 0.16 0.11 0.09 -0.02 0.05 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 2.4 2.2