Standard name
Human Ortholog
Description Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0.07 0.11 0.08 0.12 0.2 0.14 0.08 0.08 0 0.1 0.13 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.1 0.1 0.09 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Mitochondria 0 0.05 0.05 0.18 0.18 0.28 0.37 0.33 0.43 0.44 0.49 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.07 0 0.05 0 0.06 0.05 0
Nuclear Periphery 0.07 0.07 0.3 0.29 0.16 0.15 0.18 0.14 0.1 0.07 0.11 0.12 0.14 0.18 0.2 0.23 0.26 0.05 0 0.15 0.09 0.07 0.07
Nucleolus 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.89 0.86 0.8 0.76 0.75 0.64 0.53 0.49 0.48 0.38 0.4 0.83 0.84 0.88 0.8 0.75 0.78 0.88 0.87 0.77 0.78 0.78 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 11 0 2 2 0 0 0 0 0 0 2 0 1 0 0 0
Bud 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0 0 6 2 5 1 3 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 3 8 7 16 11 17 15 1 1 0 3 0 3 0 0 0 0 0 0
Cytoplasm 16 14 17 19 19 41 62 64 66 86 71 15 15 12 37 33 12 2 0 1 0 0 0
Endoplasmic Reticulum 0 0 2 4 5 21 29 78 54 39 49 2 1 4 10 15 9 1 0 1 1 3 2
Endosome 4 3 6 3 2 2 1 5 0 0 0 3 3 0 5 2 3 5 7 4 7 9 15
Golgi 30 5 8 20 27 18 17 20 5 5 5 5 4 11 5 10 20 0 0 0 3 1 2
Mitochondria 8 24 19 61 60 161 206 266 229 189 245 1 1 2 16 12 10 7 4 6 4 4 6
Nucleus 45 47 15 9 10 14 11 14 8 8 10 7 7 9 25 19 15 20 29 7 22 18 17
Nuclear Periphery 52 33 109 100 53 90 101 108 55 29 55 23 26 47 72 57 56 42 18 58 31 26 33
Nucleolus 22 9 21 12 4 9 6 14 2 1 3 7 7 4 6 8 4 12 4 1 7 10 14
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 696 430 293 259 255 373 296 393 256 163 202 156 156 234 287 187 168 744 474 299 288 286 398
Unique Cell Count 779 501 367 340 341 581 557 800 536 433 505 188 186 267 360 248 215 850 548 390 372 369 505
Labelled Cell Count 873 566 490 487 438 739 740 991 689 540 657 221 222 323 466 343 300 850 548 390 372 369 505


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 14.6 11.5 11.8 11.4 9.8 9.3 9.6 9.1 9.2 8.7 16.7 16.7 18.1 11.7 12.3 14.7 16.1 16.7 18.5
Std Deviation (1e-4) 4.5 4.7 3.6 3.3 3.2 3.1 3.1 2.7 2.7 2.6 2.3 5.2 5.2 5.3 3.8 4.4 5.3 4.9 5.1 5.8
Intensity Change (Log2) 0.05 -0.01 -0.22 -0.3 -0.26 -0.34 -0.32 -0.39 0.54 0.54 0.66 0.03 0.09 0.36 0.49 0.55 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.7 0 3.8 3.3 0 0 0 0 0 0
Cytoplasm 0.6 0.6 1.5 3.5 2.1 3.9 6.4 4.6 1.6 1.6 -0.1 2.9 3.9 0.5
Endoplasmic Reticulum 0 0 3.0 3.9 5.8 5.8 5.4 5.7 0 0 0 2.4 4.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 -0.3 0 0
Golgi 2.5 3.5 0.8 0.8 0.3 -1.5 -1.1 -1.4 0 0 1.4 -0.8 1.3 3.9
Mitochondria 5.4 5.2 8.6 11.0 10.4 12.4 12.4 13.8 -2.8 -2.8 -3.1 -0.5 -0.2 -0.3
Nucleus -1.1 -0.8 -1.5 -1.9 -2.4 -2.4 -1.9 -1.8 -0.2 -0.2 -0.5 1.7 1.9 1.5
Nuclear Periphery -0.1 -4.5 -5.2 -4.1 -6.6 -7.4 -8.6 -7.0 -4.6 -4.1 -3.5 -3.0 -1.8 -0.9
Nucleolus -1.4 -3.3 -3.6 -4.1 -3.7 -5.0 -4.7 -4.6 -1.0 -1.0 -2.7 -2.9 -1.4 -2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 -1.6 -5.1 -8.3 -9.9 -9.7 -12.0 -11.7 0.9 1.1 2.6 0 -1.3 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.014 0 0.0085 0.0145 0 0.0066 0.0078 0 0.0104 0.0134 0.021 0.0005
Bud 0.0003 0.0001 0.0005 0.0001 0.0001 0.0011 0.0003 0.0003 0.0056 0.0002 0.0003 0.0005
Bud Neck 0.0001 0 0.0006 0.0014 0 0.0008 0.0001 0 0.0005 0.0002 0.0001 0.0002
Bud Periphery 0.0005 0.0001 0.0007 0.0002 0.0005 0.0021 0.0007 0.0002 0.0114 0.0008 0.0006 0.0025
Bud Site 0.0003 0.0001 0.0011 0.0006 0 0.0018 0.0006 0.0001 0.0109 0.0003 0.0007 0.0004
Cell Periphery 0.0008 0.0006 0.001 0.0003 0.0002 0.0007 0.001 0.0004 0.0017 0.0009 0.0017 0.0008
Cytoplasm 0.0016 0.0011 0.004 0.0002 0 0.0006 0.0218 0.0028 0.0076 0.0004 0.0058 0.0054
Cytoplasmic Foci 0.0005 0.0001 0.0016 0.0078 0 0.0008 0.007 0 0.0074 0.0054 0.0058 0.0034
Eisosomes 0.0001 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0031 0.0001 0.003 0.0001 0.0001 0.0012 0.0036 0.0003 0.002 0.0006 0.0026 0.0012
Endosome 0.0138 0.0049 0.0275 0.0057 0.0018 0.0107 0.0156 0.0026 0.026 0.0142 0.0184 0.0118
Golgi 0.0019 0.0001 0.0023 0.007 0 0.0019 0.003 0 0.0041 0.0052 0.0095 0.0014
Lipid Particles 0.0012 0.0006 0.0017 0.0139 0 0.001 0.0177 0.0001 0.0071 0.0143 0.0044 0.0053
Mitochondria 0.0031 0.0009 0.0057 0.0021 0.0001 0.009 0.0049 0.0062 0.0169 0.0323 0.0056 0.0017
None 0.001 0 0.0003 0 0 0.0002 0.0052 0.0001 0.0012 0.0006 0.0007 0.0091
Nuclear Periphery 0.0324 0.0133 0.0206 0.0005 0.0014 0.0321 0.045 0.011 0.006 0.003 0.0098 0.0296
Nucleolus 0.0021 0.001 0.002 0.0002 0.0001 0.0019 0.0017 0.0002 0.0024 0.0007 0.0135 0.001
Nucleus 0.0096 0.0119 0.0087 0.0003 0.0002 0.0037 0.0076 0.0039 0.002 0.0008 0.0057 0.0028
Peroxisomes 0.0001 0 0.0002 0.0346 0 0.0003 0.0066 0 0.0036 0.0059 0.001 0.0024
Punctate Nuclear 0.0005 0 0.0002 0.0003 0 0.0003 0.0082 0 0.0026 0.0033 0.0001 0.0014
Vacuole 0.294 0.3287 0.3111 0.3643 0.2118 0.2614 0.2333 0.2398 0.2853 0.3151 0.2393 0.2319
Vacuole Periphery 0.6192 0.6364 0.5987 0.546 0.7837 0.6616 0.6082 0.732 0.5853 0.5823 0.6531 0.6868

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.2521 70.2695 85.1348 100.7659 76.7523 71.0463 92.4301 102.4934 94.9107 106.9546
Translational Efficiency 0.4915 0.4922 0.5345 0.3953 0.4448 0.5523 0.4441 0.3269 0.3994 0.376

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
115 1249 1537 73 1904 2059 1872 1484 2019 3308 3409 1557

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1267.50 1363.84 1147.45 1543.54 1071.37 1346.48 1367.55 1268.92 1082.54 1353.03 1268.31 1281.80
Standard Deviation 203.56 270.45 279.82 294.08 205.97 255.68 247.62 240.99 210.79 261.49 284.55 250.55
Intensity Change Log 2 0.105689 -0.143554 0.284257 0.329736 0.352137 0.244144 0.212652 0.104747 0.266021

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000093 0.000054 0.000153 0.000037 0.000011 0.000032 0.000024 0.000067 0.000016 0.000040 0.000082 0.000065
Bud Neck 0.001385 0.000606 0.001260 0.001570 0.000151 0.000265 0.000590 0.001408 0.000221 0.000394 0.000892 0.001415
Bud Site 0.001933 0.000890 0.003537 0.001830 0.001006 0.000616 0.000504 0.002267 0.001058 0.000719 0.001872 0.002247
Cell Periphery 0.000498 0.000643 0.000746 0.000219 0.001244 0.000639 0.001276 0.000526 0.001202 0.000640 0.001037 0.000511
Cytoplasm 0.000214 0.000183 0.001673 0.000165 0.000280 0.000078 0.000071 0.000402 0.000277 0.000118 0.000793 0.000391
Cytoplasmic Foci 0.005983 0.000342 0.002011 0.001160 0.000170 0.000377 0.000372 0.001600 0.000502 0.000363 0.001111 0.001579
Eisosomes 0.000018 0.000015 0.000022 0.000008 0.000002 0.000016 0.000066 0.000024 0.000003 0.000016 0.000046 0.000023
Endoplasmic Reticulum 0.001130 0.000510 0.000427 0.000141 0.001288 0.000552 0.000357 0.000716 0.001279 0.000536 0.000389 0.000689
Endosome 0.016204 0.001837 0.005408 0.006015 0.000803 0.001244 0.001617 0.009264 0.001680 0.001468 0.003326 0.009112
Golgi 0.082712 0.000504 0.005191 0.032899 0.000290 0.000757 0.000831 0.007941 0.004985 0.000661 0.002797 0.009111
Lipid Particles 0.000708 0.000488 0.000737 0.000543 0.000133 0.000695 0.001446 0.001165 0.000166 0.000616 0.001127 0.001136
Mitochondria 0.028165 0.005629 0.011111 0.005015 0.003002 0.010387 0.008944 0.018428 0.004435 0.008590 0.009921 0.017799
Mitotic Spindle 0.000578 0.000312 0.000293 0.000111 0.000023 0.000074 0.000430 0.000367 0.000054 0.000164 0.000368 0.000355
None 0.000378 0.000523 0.001174 0.000132 0.000136 0.000387 0.000416 0.000508 0.000150 0.000438 0.000758 0.000490
Nuclear Periphery 0.001419 0.001539 0.001666 0.001468 0.000312 0.001988 0.001020 0.003492 0.000375 0.001818 0.001311 0.003397
Nuclear Periphery Foci 0.001095 0.000748 0.001133 0.000976 0.000091 0.000981 0.000627 0.002518 0.000149 0.000893 0.000855 0.002445
Nucleolus 0.002342 0.003130 0.005626 0.003113 0.000225 0.002791 0.004248 0.004001 0.000345 0.002919 0.004869 0.003959
Nucleus 0.001742 0.001866 0.004015 0.001138 0.000423 0.002224 0.001288 0.002963 0.000498 0.002089 0.002517 0.002877
Peroxisomes 0.000253 0.000434 0.000749 0.000254 0.000031 0.000201 0.000142 0.000230 0.000044 0.000289 0.000416 0.000232
Vacuole 0.390882* 0.225421* 0.208774* 0.352285* 0.124424 0.236403* 0.403662* 0.388137* 0.139601 0.232256* 0.315794* 0.386456*
Vacuole Periphery 0.462268* 0.754329* 0.744294* 0.590921* 0.865954* 0.739294* 0.572066* 0.553977* 0.842960* 0.744971* 0.649718* 0.555710*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.95 -1.50 1.59 0.75 4.10 -3.62 -2.92 -3.59 -2.18 -2.71 -2.74 -5.32 -3.33 -1.46 0.97
Bud Neck 1.36 0.15 -0.39 -1.84 -0.65 -1.64 -5.31 -7.11 -6.70 -4.66 -1.74 -6.05 -6.93 -5.80 -2.81
Bud Site 1.52 -1.83 -0.09 -1.39 1.55 1.25 1.95 -3.05 -4.27 -5.06 1.18 -2.22 -3.02 -4.24 -0.92
Cell Periphery -0.83 -1.22 1.60 2.87 2.91 3.49 -0.16 4.07 0.95 5.02 3.54 0.95 4.13 1.23 4.18
Cytoplasm 0.31 -4.03 0.27 -0.04 4.12 2.38 2.04 -1.16 -2.12 -2.06 1.92 -3.06 -1.16 -1.95 1.24
Cytoplasmic Foci 2.32 1.62 1.95 -2.15 1.76 -1.18 -3.14 -3.78 -2.97 -3.23 0.76 -3.13 -2.80 -3.24 -1.25
Eisosomes 0.66 -1.01 2.42 2.80 5.17 -9.12 -13.11 -10.64 -3.14 7.97 -10.61 -15.06 -10.25 -3.32 6.64
Endoplasmic Reticulum 1.26 1.42 2.02 5.87 5.21 4.22 5.89 3.25 -1.51 -4.46 4.66 5.83 3.50 -1.68 -3.81
Endosome 3.71 2.76 2.43 -2.85 -0.47 -2.06 -3.09 -10.48 -9.75 -9.13 0.67 -4.02 -9.22 -9.67 -6.93
Golgi 4.16 3.92 2.11 -2.42 -2.06 -1.97 -5.86 -6.88 -6.33 -6.38 3.58 1.77 -2.39 -6.79 -4.97
Lipid Particles 0.65 -0.11 0.39 -0.18 0.66 -6.91 -12.05 -6.35 -2.65 1.36 -6.54 -11.33 -6.20 -3.12 -0.11
Mitochondria 3.14 2.37 3.17 0.31 2.77 -4.95 -4.23 -7.80 -3.55 -4.29 -3.41 -4.65 -6.92 -4.63 -4.00
Mitotic Spindle 0.71 0.79 1.59 0.98 1.94 -1.77 -1.30 -2.83 -2.38 0.18 -1.40 -1.84 -2.58 -1.41 0.10
None -0.93 -4.63 3.44 2.74 6.47 -4.75 -8.22 -4.52 -1.29 -1.08 -4.52 -8.02 -4.34 -0.54 2.53
Nuclear Periphery -0.19 -0.52 -0.08 0.11 0.42 -5.22 -5.50 -4.85 -2.07 -3.72 -5.23 -6.49 -4.82 -2.32 -3.27
Nuclear Periphery Foci 0.99 -0.22 0.30 -0.69 0.59 -3.36 -8.51 -5.04 -2.81 -3.90 -4.05 -7.63 -5.00 -3.15 -3.38
Nucleolus -0.52 -2.21 -0.45 0.02 2.02 -5.45 -8.02 -6.84 -1.68 0.32 -6.27 -10.98 -6.77 -1.58 1.39
Nucleus -0.12 -1.94 0.51 1.23 3.76 -3.00 -5.10 -4.84 -1.06 -3.32 -3.81 -6.13 -4.73 -1.37 -0.66
Peroxisomes -0.68 -2.10 0.01 0.70 2.18 -2.21 -4.02 -6.22 -0.37 -2.25 -2.38 -3.97 -5.92 0.55 1.91
Vacuole 6.71 7.43 0.94 -4.87 -5.51 -18.15 -41.08 -35.84 -19.80 1.90 -16.45 -29.89 -33.58 -22.14 -9.82
Vacuole Periphery -11.08 -10.68 -3.09 5.37 5.02 18.79* 41.02* 38.65* 21.88 2.09 15.56 29.51 35.05 24.55 11.82