Standard name
Human Ortholog
Description Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.96 0.97 0.93 – 0.75 0.75 0.77 0.71 0.72 1.0 1.0 1.0 0.92 0.96 0.96 0.95 0.96 0.92 0.93 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.12 0.05 0.25 – 0.39 0.48 0.38 0.5 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud 0 1 1 4 1 2 – 6 11 12 2 6 0 0 0 0 0 0 1 0 0 0 3 2
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 1 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 1 1 – 1 1 2 2 1 1 1 0 6 3 4 0 0 0 0 0 0
Cytoplasm 410 283 108 271 386 433 – 517 466 529 265 340 176 322 336 121 163 106 403 274 102 76 228 253
Endoplasmic Reticulum 1 1 0 2 1 0 – 1 0 0 1 2 0 1 1 2 5 5 0 0 0 0 0 1
Endosome 1 0 0 1 2 0 – 0 1 2 0 0 0 0 0 1 1 0 3 1 0 0 2 0
Golgi 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 1 2 0 1 0 0 0 1
Mitochondria 5 2 9 34 19 117 – 266 301 262 186 243 0 0 0 4 4 3 4 2 3 1 3 1
Nucleus 0 0 0 0 0 2 – 14 5 6 4 3 1 0 0 3 0 1 1 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 – 8 1 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 0 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 – 1 0 1 3 2 0 0 0 0 0 0 4 0 0 0 1 0
Vac/Vac Membrane 0 1 1 0 1 5 – 15 23 17 7 5 0 0 0 2 3 1 1 2 0 0 3 3
Unique Cell Count 416 287 111 282 398 467 687 623 689 371 472 176 323 337 131 169 110 425 287 112 82 252 277
Labelled Cell Count 418 290 121 315 411 561 829 812 837 471 605 178 324 337 140 180 122 425 287 112 82 252 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.9 4.0 3.7 4.4 3.6 – 3.7 3.5 3.7 3.3 3.3 5.7 5.7 5.7 6.6 6.9 6.6 4.8 5.8 5.8
Std Deviation (1e-4) 0.8 0.8 2.2 1.0 1.2 1.2 – 1.1 2.0 1.1 0.9 0.6 1.0 0.8 0.9 1.2 1.5 1.4 0.9 1.0 1.0
Intensity Change (Log2) – – – -0.1 0.12 -0.15 – -0.13 -0.19 -0.13 -0.28 -0.29 0.52 0.51 0.52 0.71 0.79 0.72 0.27 0.52 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -0.2 -1.8 – -5.2 -5.3 -5.0 -5.7 -5.7 2.2 2.3 2.3 -1.7 -0.4 -0.4
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 -1.7 -2.2 -1.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4832 1.4422 1.3056 1.1799 0.4889 0.9837 0.2597 0.7858 0.562 0.5367 0.3565 0.7991 2.0843 2.7511 2.4908 2.5533 1.7809 2.5471
Actin 0.0431 0.0004 0.0268 0.0034 0.0459 0.0055 0.0233 0.001 0.0044 0.0095 0.0009 0.0026 0.022 0.0003 0.0167 0.0086 0.0001 0.0013
Bud 0.0008 0.0006 0.0023 0.0002 0.0016 0.0001 0.001 0.0006 0.0019 0.002 0.0003 0.0001 0.0007 0.0065 0.0001 0.0004 0 0.0004
Bud Neck 0.0036 0.0001 0.0025 0.0004 0.0016 0.0008 0.0037 0.0002 0.0004 0.0008 0.0003 0.0004 0.0018 0.0001 0.0006 0.0019 0.0001 0.0038
Bud Periphery 0.0013 0.0007 0.0023 0.0001 0.0023 0.0001 0.002 0.0007 0.001 0.0013 0.0008 0.0002 0.0009 0.0014 0.0001 0.0004 0 0.0007
Bud Site 0.0025 0.0005 0.0084 0.0021 0.0027 0.0001 0.0099 0.0047 0.0059 0.0085 0.0006 0.0003 0.0146 0.0023 0.0014 0.0023 0 0.002
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0008 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.4099 0.613 0.5022 0.5103 0.4778 0.6568 0.5283 0.6941 0.6414 0.8346 0.4686 0.7326 0.4073 0.607 0.6596 0.644 0.7609 0.6894
Cytoplasmic Foci 0.02 0.006 0.0205 0.0378 0.0563 0.0073 0.0282 0.0043 0.0148 0.0234 0.0071 0.0064 0.0353 0.0038 0.0039 0.0365 0.0032 0.0052
Eisosomes 0.0004 0 0.0002 0.0001 0.0002 0 0.0002 0.0001 0 0 0.0001 0 0.0003 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0051 0.0022 0.0036 0.0038 0.0021 0.0029 0.0052 0.003 0.0044 0.0011 0.0035 0.0012 0.0033 0.0027 0.0012 0.0024 0.0014 0.0016
Endosome 0.0219 0.0017 0.0144 0.0151 0.0375 0.0092 0.0374 0.0017 0.0135 0.013 0.0721 0.0208 0.0289 0.0009 0.0044 0.0116 0.0013 0.0038
Golgi 0.0052 0.0001 0.0059 0.0048 0.0053 0.002 0.0097 0.0004 0.0023 0.0095 0.0265 0.0051 0.008 0.0001 0.0041 0.0132 0 0.0014
Lipid Particles 0.0131 0.0002 0.0112 0.025 0.0132 0.0006 0.0098 0.0002 0.0017 0.0012 0.0069 0.0079 0.0146 0.0001 0.0044 0.0138 0 0.0008
Mitochondria 0.0028 0.0001 0.0076 0.018 0.0022 0.0004 0.0047 0.005 0.0008 0.0047 0.1152 0.0086 0.0153 0.0001 0.0018 0.031 0.0001 0.0009
None 0.419 0.3706 0.3335 0.3461 0.3059 0.3066 0.2949 0.2799 0.2834 0.0814 0.2754 0.2065 0.417 0.3712 0.2957 0.2097 0.2305 0.2839
Nuclear Periphery 0.0148 0.0006 0.0088 0.0025 0.0035 0.0022 0.0047 0.0007 0.0048 0.0005 0.0084 0.0006 0.0039 0.0006 0.0005 0.0011 0.0004 0.0004
Nucleolus 0.0006 0.0001 0.0017 0.0004 0.0006 0.0001 0.0008 0.0001 0.0003 0.0001 0.0003 0.0001 0.0009 0.0001 0 0.0005 0 0.0008
Nucleus 0.0174 0.0015 0.0049 0.0029 0.0018 0.0033 0.0037 0.0015 0.0088 0.0013 0.0018 0.0022 0.002 0.0013 0.0012 0.004 0.0012 0.0013
Peroxisomes 0.01 0.0001 0.0245 0.0139 0.0202 0.0001 0.0086 0.0002 0.0063 0.0049 0.0012 0.0013 0.0065 0.0001 0.0029 0.0157 0 0.0005
Punctate Nuclear 0.0053 0.0005 0.0153 0.0105 0.0176 0.0011 0.0137 0.0004 0.0022 0.0007 0.0014 0.0004 0.0038 0.0006 0.0003 0.0016 0.0002 0.0007
Vacuole 0.0019 0.0009 0.002 0.0019 0.001 0.0006 0.0082 0.001 0.0013 0.001 0.005 0.0018 0.0108 0.0004 0.0007 0.0009 0.0003 0.0009
Vacuole Periphery 0.001 0.0001 0.0011 0.0005 0.0005 0.0002 0.0014 0.0001 0.0004 0.0003 0.0033 0.0008 0.0013 0.0001 0.0002 0.0003 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.9425 50.5834 43.7909 63.9692 73.3647 108.3942 59.669 58.9705 71.3244 62.6736
Translational Efficiency 1.8686 2.0284 2.3206 1.9051 1.6488 1.2505 2.0865 1.823 1.6405 1.6468

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘