Standard name
Description Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.96 0.97 0.93 0.75 0.75 0.77 0.71 0.72 1.0 1.0 1.0 0.92 0.96 0.96 0.95 0.96 0.92 0.93 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.12 0.05 0.25 0.39 0.48 0.38 0.5 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud 0 1 1 4 1 2 6 11 12 2 6 0 0 0 0 0 0 1 0 0 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0
Cell Periphery 1 1 2 0 1 1 1 1 2 2 1 1 1 0 6 3 4 0 0 0 0 0 0
Cytoplasm 410 283 108 271 386 433 517 466 529 265 340 176 322 336 121 163 106 403 274 102 76 228 253
Endoplasmic Reticulum 1 1 0 2 1 0 1 0 0 1 2 0 1 1 2 5 5 0 0 0 0 0 1
Endosome 1 0 0 1 2 0 0 1 2 0 0 0 0 0 1 1 0 3 1 0 0 2 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 0 1 0 0 0 1
Mitochondria 5 2 9 34 19 117 266 301 262 186 243 0 0 0 4 4 3 4 2 3 1 3 1
Nucleus 0 0 0 0 0 2 14 5 6 4 3 1 0 0 3 0 1 1 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 8 1 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 1 0 1 3 2 0 0 0 0 0 0 4 0 0 0 1 0
Vac/Vac Membrane 0 1 1 0 1 5 15 23 17 7 5 0 0 0 2 3 1 1 2 0 0 3 3
Unique Cell Count 416 287 111 282 398 467 687 623 689 371 472 176 323 337 131 169 110 425 287 112 82 252 277
Labelled Cell Count 418 290 121 315 411 561 829 812 837 471 605 178 324 337 140 180 122 425 287 112 82 252 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.9 4.0 3.7 4.4 3.6 3.7 3.5 3.7 3.3 3.3 5.7 5.7 5.7 6.6 6.9 6.6 4.8 5.8 5.8
Std Deviation (1e-4) 0.8 0.8 2.2 1.0 1.2 1.2 1.1 2.0 1.1 0.9 0.6 1.0 0.8 0.9 1.2 1.5 1.4 0.9 1.0 1.0
Intensity Change (Log2) -0.1 0.12 -0.15 -0.13 -0.19 -0.13 -0.28 -0.29 0.52 0.51 0.52 0.71 0.79 0.72 0.27 0.52 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -0.2 -1.8 -5.2 -5.3 -5.0 -5.7 -5.7 2.2 2.3 2.3 -1.7 -0.4 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 -1.7 -2.2 -1.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available