Standard name
Human Ortholog
Description Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.96 0.97 0.93 – 0.75 0.75 0.77 0.71 0.72 1.0 1.0 1.0 0.92 0.96 0.96 0.95 0.96 0.92 0.93 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.12 0.05 0.25 – 0.39 0.48 0.38 0.5 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud 0 1 1 4 1 2 – 6 11 12 2 6 0 0 0 0 0 0 1 0 0 0 3 2
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 1 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 1 1 – 1 1 2 2 1 1 1 0 6 3 4 0 0 0 0 0 0
Cytoplasm 410 283 108 271 386 433 – 517 466 529 265 340 176 322 336 121 163 106 403 274 102 76 228 253
Endoplasmic Reticulum 1 1 0 2 1 0 – 1 0 0 1 2 0 1 1 2 5 5 0 0 0 0 0 1
Endosome 1 0 0 1 2 0 – 0 1 2 0 0 0 0 0 1 1 0 3 1 0 0 2 0
Golgi 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 1 2 0 1 0 0 0 1
Mitochondria 5 2 9 34 19 117 – 266 301 262 186 243 0 0 0 4 4 3 4 2 3 1 3 1
Nucleus 0 0 0 0 0 2 – 14 5 6 4 3 1 0 0 3 0 1 1 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 – 8 1 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 0 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 – 1 0 1 3 2 0 0 0 0 0 0 4 0 0 0 1 0
Vac/Vac Membrane 0 1 1 0 1 5 – 15 23 17 7 5 0 0 0 2 3 1 1 2 0 0 3 3
Unique Cell Count 416 287 111 282 398 467 687 623 689 371 472 176 323 337 131 169 110 425 287 112 82 252 277
Labelled Cell Count 418 290 121 315 411 561 829 812 837 471 605 178 324 337 140 180 122 425 287 112 82 252 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.9 4.0 3.7 4.4 3.6 – 3.7 3.5 3.7 3.3 3.3 5.7 5.7 5.7 6.6 6.9 6.6 4.8 5.8 5.8
Std Deviation (1e-4) 0.8 0.8 2.2 1.0 1.2 1.2 – 1.1 2.0 1.1 0.9 0.6 1.0 0.8 0.9 1.2 1.5 1.4 0.9 1.0 1.0
Intensity Change (Log2) – – – -0.1 0.12 -0.15 – -0.13 -0.19 -0.13 -0.28 -0.29 0.52 0.51 0.52 0.71 0.79 0.72 0.27 0.52 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 -0.2 -1.8 – -5.2 -5.3 -5.0 -5.7 -5.7 2.2 2.3 2.3 -1.7 -0.4 -0.4
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 -1.7 -2.2 -1.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4832 1.4422 1.3056 1.1799 0.4889 0.9837 0.2597 0.7858 0.562 0.5367 0.3565 0.7991 2.0843 2.7511 2.4908 2.5533 1.7809 2.5471
Actin 0.0431 0.0004 0.0268 0.0034 0.0459 0.0055 0.0233 0.001 0.0044 0.0095 0.0009 0.0026 0.022 0.0003 0.0167 0.0086 0.0001 0.0013
Bud 0.0008 0.0006 0.0023 0.0002 0.0016 0.0001 0.001 0.0006 0.0019 0.002 0.0003 0.0001 0.0007 0.0065 0.0001 0.0004 0 0.0004
Bud Neck 0.0036 0.0001 0.0025 0.0004 0.0016 0.0008 0.0037 0.0002 0.0004 0.0008 0.0003 0.0004 0.0018 0.0001 0.0006 0.0019 0.0001 0.0038
Bud Periphery 0.0013 0.0007 0.0023 0.0001 0.0023 0.0001 0.002 0.0007 0.001 0.0013 0.0008 0.0002 0.0009 0.0014 0.0001 0.0004 0 0.0007
Bud Site 0.0025 0.0005 0.0084 0.0021 0.0027 0.0001 0.0099 0.0047 0.0059 0.0085 0.0006 0.0003 0.0146 0.0023 0.0014 0.0023 0 0.002
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0008 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.4099 0.613 0.5022 0.5103 0.4778 0.6568 0.5283 0.6941 0.6414 0.8346 0.4686 0.7326 0.4073 0.607 0.6596 0.644 0.7609 0.6894
Cytoplasmic Foci 0.02 0.006 0.0205 0.0378 0.0563 0.0073 0.0282 0.0043 0.0148 0.0234 0.0071 0.0064 0.0353 0.0038 0.0039 0.0365 0.0032 0.0052
Eisosomes 0.0004 0 0.0002 0.0001 0.0002 0 0.0002 0.0001 0 0 0.0001 0 0.0003 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0051 0.0022 0.0036 0.0038 0.0021 0.0029 0.0052 0.003 0.0044 0.0011 0.0035 0.0012 0.0033 0.0027 0.0012 0.0024 0.0014 0.0016
Endosome 0.0219 0.0017 0.0144 0.0151 0.0375 0.0092 0.0374 0.0017 0.0135 0.013 0.0721 0.0208 0.0289 0.0009 0.0044 0.0116 0.0013 0.0038
Golgi 0.0052 0.0001 0.0059 0.0048 0.0053 0.002 0.0097 0.0004 0.0023 0.0095 0.0265 0.0051 0.008 0.0001 0.0041 0.0132 0 0.0014
Lipid Particles 0.0131 0.0002 0.0112 0.025 0.0132 0.0006 0.0098 0.0002 0.0017 0.0012 0.0069 0.0079 0.0146 0.0001 0.0044 0.0138 0 0.0008
Mitochondria 0.0028 0.0001 0.0076 0.018 0.0022 0.0004 0.0047 0.005 0.0008 0.0047 0.1152 0.0086 0.0153 0.0001 0.0018 0.031 0.0001 0.0009
None 0.419 0.3706 0.3335 0.3461 0.3059 0.3066 0.2949 0.2799 0.2834 0.0814 0.2754 0.2065 0.417 0.3712 0.2957 0.2097 0.2305 0.2839
Nuclear Periphery 0.0148 0.0006 0.0088 0.0025 0.0035 0.0022 0.0047 0.0007 0.0048 0.0005 0.0084 0.0006 0.0039 0.0006 0.0005 0.0011 0.0004 0.0004
Nucleolus 0.0006 0.0001 0.0017 0.0004 0.0006 0.0001 0.0008 0.0001 0.0003 0.0001 0.0003 0.0001 0.0009 0.0001 0 0.0005 0 0.0008
Nucleus 0.0174 0.0015 0.0049 0.0029 0.0018 0.0033 0.0037 0.0015 0.0088 0.0013 0.0018 0.0022 0.002 0.0013 0.0012 0.004 0.0012 0.0013
Peroxisomes 0.01 0.0001 0.0245 0.0139 0.0202 0.0001 0.0086 0.0002 0.0063 0.0049 0.0012 0.0013 0.0065 0.0001 0.0029 0.0157 0 0.0005
Punctate Nuclear 0.0053 0.0005 0.0153 0.0105 0.0176 0.0011 0.0137 0.0004 0.0022 0.0007 0.0014 0.0004 0.0038 0.0006 0.0003 0.0016 0.0002 0.0007
Vacuole 0.0019 0.0009 0.002 0.0019 0.001 0.0006 0.0082 0.001 0.0013 0.001 0.005 0.0018 0.0108 0.0004 0.0007 0.0009 0.0003 0.0009
Vacuole Periphery 0.001 0.0001 0.0011 0.0005 0.0005 0.0002 0.0014 0.0001 0.0004 0.0003 0.0033 0.0008 0.0013 0.0001 0.0002 0.0003 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 58.9425 50.5834 43.7909 63.9692 73.3647 108.3942 59.669 58.9705 71.3244 62.6736
Translational Efficiency 1.8686 2.0284 2.3206 1.9051 1.6488 1.2505 2.0865 1.823 1.6405 1.6468

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
121 1106 1467 404 1750 654 1006 76 1871 1760 2473 480

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 689.80 764.47 915.25 1061.18 717.74 822.29 930.61 1004.85 715.93 785.96 921.50 1052.26
Standard Deviation 80.43 110.15 129.91 159.84 84.79 102.49 117.38 122.85 84.79 110.95 125.19 155.93
Intensity Change Log 2 — 0.148282 0.407988 0.621419 — 0.196186 0.374715 0.485447 — 0.172908 0.391117 0.553685

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000434 0.000421 0.001917 0.000107 0.000729 0.000448 0.017637 0.000104 0.000544 0.000432 0.004406
Bud Neck 0.005250 0.017021 0.002576 0.003286 0.008537 0.026951 0.004840 0.005599 0.008325 0.020711 0.003497 0.003652
Bud Site 0.001315 0.014075 0.007291 0.036211 0.003117 0.021192 0.020048 0.194008* 0.003000 0.016720 0.012481 0.061195
Cell Periphery 0.000224 0.000097 0.000095 0.000170 0.000134 0.000178 0.000103 0.000128 0.000140 0.000127 0.000098 0.000163
Cytoplasm 0.505186* 0.570479* 0.522880* 0.364182* 0.562372* 0.425697* 0.654431* 0.265550* 0.558673* 0.516680* 0.576394* 0.348566*
Cytoplasmic Foci 0.233536* 0.166523 0.011475 0.039838 0.184751* 0.204500* 0.017143 0.023005 0.187906* 0.180635* 0.013781 0.037173
Eisosomes 0.000191 0.000070 0.000024 0.000042 0.000158 0.000128 0.000028 0.000055 0.000160 0.000091 0.000025 0.000044
Endoplasmic Reticulum 0.000414 0.000655 0.002359 0.003162 0.000443 0.000705 0.001804 0.000926 0.000441 0.000673 0.002133 0.002808
Endosome 0.008968 0.015520 0.001663 0.034427 0.006494 0.029992 0.003948 0.004756 0.006654 0.020898 0.002592 0.029729
Golgi 0.000488 0.002721 0.000190 0.046469 0.001011 0.005059 0.001805 0.000886 0.000977 0.003590 0.000847 0.039252
Lipid Particles 0.009439 0.003412 0.000749 0.003579 0.004509 0.003604 0.001060 0.000880 0.004828 0.003483 0.000876 0.003152
Mitochondria 0.001451 0.005550 0.000421 0.031304 0.005097 0.009352 0.001162 0.001604 0.004861 0.006963 0.000722 0.026602
Mitotic Spindle 0.000105 0.008679 0.002958 0.099214 0.001204 0.004835 0.020361 0.069415 0.001133 0.007250 0.010037 0.094496
None 0.003280 0.003320 0.004973 0.004880 0.002038 0.001128 0.003601 0.002350 0.002118 0.002505 0.004415 0.004479
Nuclear Periphery 0.000764 0.000277 0.001161 0.001646 0.000266 0.000329 0.000801 0.000464 0.000299 0.000297 0.001015 0.001458
Nuclear Periphery Foci 0.000545 0.000683 0.001376 0.003815 0.000331 0.000358 0.001866 0.001575 0.000345 0.000562 0.001576 0.003460
Nucleolus 0.002354 0.001683 0.000169 0.000111 0.000640 0.000926 0.000134 0.000226 0.000751 0.001402 0.000155 0.000129
Nucleus 0.173302* 0.093904 0.387128* 0.099577 0.164317* 0.087985 0.168780* 0.025883 0.164898* 0.091704 0.298306* 0.087908
Peroxisomes 0.002942 0.008208 0.000443 0.001027 0.002192 0.018989 0.000442 0.026488 0.002240 0.012214 0.000442 0.005059
Vacuole 0.049741 0.085201 0.051551 0.212110* 0.051819 0.154675 0.095551 0.358408* 0.051685 0.111016 0.069450 0.235274*
Vacuole Periphery 0.000450 0.001490 0.000098 0.013034 0.000463 0.002689 0.001646 0.000158 0.000462 0.001935 0.000727 0.010995

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.35 -5.01 -5.90 -1.35 -1.98 -3.29 -3.15 -1.70 -1.12 -1.41 -3.51 -5.18 -4.05 -1.56 -2.30
Bud Neck -6.10 1.86 1.98 8.56 0.10 -7.07 4.12 7.30 9.63 2.10 -8.09 6.40 8.08 12.70 1.47
Bud Site -9.50 -4.38 -5.06 -0.25 -2.90 -6.11 -4.47 -2.17 -0.75 -1.37 -9.44 -5.00 -4.70 -0.22 -2.58
Cell Periphery 1.87 1.73 1.64 -1.57 -0.67 -1.25 3.84 6.07 3.69 3.73 1.06 3.81 3.08 1.54 -0.33
Cytoplasm -2.68 2.29 9.05 18.98 12.10 10.86 0.78 9.42 4.76 9.02 5.14 8.33 22.03 17.51 17.04
Cytoplasmic Foci 3.86 13.28 12.87 26.56 -3.44 -1.86 37.46 29.38 23.09 -0.54 1.51 40.47 37.77 35.65 -2.79
Eisosomes 2.58 3.38 3.19 7.38 -3.63 2.33 10.30 8.51 9.38 -3.43 5.70 10.84 9.90 12.55 -4.85
Endoplasmic Reticulum -1.63 -13.39 -7.02 -6.49 6.46 -1.39 -7.50 -3.55 -1.96 2.19 -2.62 -16.56 -8.99 -6.53 5.49
Endosome -2.57 4.16 1.77 5.30 -2.60 -9.40 6.76 6.79 11.16 -0.20 -10.86 9.40 1.31 9.23 -2.47
Golgi -5.22 2.51 -2.28 -1.27 -2.44 -3.25 0.58 4.82 4.01 1.63 -4.77 2.67 -1.98 -0.63 -2.29
Lipid Particles 2.28 3.13 2.66 2.04 -4.36 1.69 8.81 8.68 5.22 -0.06 2.81 9.55 5.88 3.25 -4.24
Mitochondria -2.36 1.01 -1.18 0.61 -2.03 -1.50 3.26 3.65 4.04 2.39 -1.08 3.47 0.73 1.74 -1.91
Mitotic Spindle -4.43 -4.86 -5.58 -4.33 -4.91 -2.44 -5.32 -2.01 -1.72 -0.86 -4.37 -6.18 -5.79 -4.81 -4.52
None 0.12 -0.55 -0.06 -0.42 1.56 2.09 -3.85 0.39 -1.28 3.57 -0.42 -4.70 -1.86 -1.44 1.99
Nuclear Periphery 1.98 -3.35 -3.68 -13.47 -1.10 -1.82 -19.29 -5.64 -5.10 0.76 -0.16 -24.78 -14.02 -14.11 -1.86
Nuclear Periphery Foci -0.51 -4.42 -6.67 -9.33 -3.65 -0.52 -9.94 -5.14 -5.14 0.99 -3.11 -12.66 -11.57 -10.74 -3.35
Nucleolus 0.39 1.23 1.25 3.43 1.56 -1.16 3.24 3.26 5.76 0.32 -1.89 3.20 3.31 4.27 1.28
Nucleus 5.14 -15.38 3.29 -2.70 28.88 12.03 -7.82 11.31 3.36 15.32 15.29 -27.04 7.52 -2.07 27.13
Peroxisomes -4.37 3.48 3.40 8.62 -0.38 -5.83 9.55 -0.16 4.91 -1.33 -8.14 10.11 4.66 9.31 -1.43
Vacuole -4.69 -4.51 -25.29 -24.77 -24.82 -14.39 -16.87 -14.49 -11.17 -11.36 -15.60 -16.71 -31.11 -25.89 -26.01
Vacuole Periphery -2.72 3.52 -1.27 -0.18 -1.67 -3.07 -0.64 6.42 3.60 1.52 -4.11 0.63 -1.19 0.58 -1.31
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rub1

Rub1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available