Standard name | |
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Human Ortholog | |
Description | Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Bud | 0 | 1 | 1 | 4 | 1 | 2 | – | 6 | 11 | 12 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 2 | 0 | 1 | 1 | – | 1 | 1 | 2 | 2 | 1 | 1 | 1 | 0 | 6 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 410 | 283 | 108 | 271 | 386 | 433 | – | 517 | 466 | 529 | 265 | 340 | 176 | 322 | 336 | 121 | 163 | 106 | 403 | 274 | 102 | 76 | 228 | 253 |
Endoplasmic Reticulum | 1 | 1 | 0 | 2 | 1 | 0 | – | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 2 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 0 | 0 | 1 | 2 | 0 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 5 | 2 | 9 | 34 | 19 | 117 | – | 266 | 301 | 262 | 186 | 243 | 0 | 0 | 0 | 4 | 4 | 3 | 4 | 2 | 3 | 1 | 3 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | – | 14 | 5 | 6 | 4 | 3 | 1 | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | – | 8 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | – | 1 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 1 | 5 | – | 15 | 23 | 17 | 7 | 5 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 2 | 0 | 0 | 3 | 3 |
Unique Cell Count | 416 | 287 | 111 | 282 | 398 | 467 | 687 | 623 | 689 | 371 | 472 | 176 | 323 | 337 | 131 | 169 | 110 | 425 | 287 | 112 | 82 | 252 | 277 | |
Labelled Cell Count | 418 | 290 | 121 | 315 | 411 | 561 | 829 | 812 | 837 | 471 | 605 | 178 | 324 | 337 | 140 | 180 | 122 | 425 | 287 | 112 | 82 | 252 | 277 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.9 | 4.0 | 3.7 | 4.4 | 3.6 | – | 3.7 | 3.5 | 3.7 | 3.3 | 3.3 | 5.7 | 5.7 | 5.7 | 6.6 | 6.9 | 6.6 | 4.8 | 5.8 | 5.8 |
Std Deviation (1e-4) | 0.8 | 0.8 | 2.2 | 1.0 | 1.2 | 1.2 | – | 1.1 | 2.0 | 1.1 | 0.9 | 0.6 | 1.0 | 0.8 | 0.9 | 1.2 | 1.5 | 1.4 | 0.9 | 1.0 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.12 | -0.15 | – | -0.13 | -0.19 | -0.13 | -0.28 | -0.29 | 0.52 | 0.51 | 0.52 | 0.71 | 0.79 | 0.72 | 0.27 | 0.52 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -0.2 | -1.8 | – | -5.2 | -5.3 | -5.0 | -5.7 | -5.7 | 2.2 | 2.3 | 2.3 | -1.7 | -0.4 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | -2.2 | -1.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4832 | 1.4422 | 1.3056 | 1.1799 | 0.4889 | 0.9837 | 0.2597 | 0.7858 | 0.562 | 0.5367 | 0.3565 | 0.7991 | 2.0843 | 2.7511 | 2.4908 | 2.5533 | 1.7809 | 2.5471 |
Actin | 0.0431 | 0.0004 | 0.0268 | 0.0034 | 0.0459 | 0.0055 | 0.0233 | 0.001 | 0.0044 | 0.0095 | 0.0009 | 0.0026 | 0.022 | 0.0003 | 0.0167 | 0.0086 | 0.0001 | 0.0013 |
Bud | 0.0008 | 0.0006 | 0.0023 | 0.0002 | 0.0016 | 0.0001 | 0.001 | 0.0006 | 0.0019 | 0.002 | 0.0003 | 0.0001 | 0.0007 | 0.0065 | 0.0001 | 0.0004 | 0 | 0.0004 |
Bud Neck | 0.0036 | 0.0001 | 0.0025 | 0.0004 | 0.0016 | 0.0008 | 0.0037 | 0.0002 | 0.0004 | 0.0008 | 0.0003 | 0.0004 | 0.0018 | 0.0001 | 0.0006 | 0.0019 | 0.0001 | 0.0038 |
Bud Periphery | 0.0013 | 0.0007 | 0.0023 | 0.0001 | 0.0023 | 0.0001 | 0.002 | 0.0007 | 0.001 | 0.0013 | 0.0008 | 0.0002 | 0.0009 | 0.0014 | 0.0001 | 0.0004 | 0 | 0.0007 |
Bud Site | 0.0025 | 0.0005 | 0.0084 | 0.0021 | 0.0027 | 0.0001 | 0.0099 | 0.0047 | 0.0059 | 0.0085 | 0.0006 | 0.0003 | 0.0146 | 0.0023 | 0.0014 | 0.0023 | 0 | 0.002 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.4099 | 0.613 | 0.5022 | 0.5103 | 0.4778 | 0.6568 | 0.5283 | 0.6941 | 0.6414 | 0.8346 | 0.4686 | 0.7326 | 0.4073 | 0.607 | 0.6596 | 0.644 | 0.7609 | 0.6894 |
Cytoplasmic Foci | 0.02 | 0.006 | 0.0205 | 0.0378 | 0.0563 | 0.0073 | 0.0282 | 0.0043 | 0.0148 | 0.0234 | 0.0071 | 0.0064 | 0.0353 | 0.0038 | 0.0039 | 0.0365 | 0.0032 | 0.0052 |
Eisosomes | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0051 | 0.0022 | 0.0036 | 0.0038 | 0.0021 | 0.0029 | 0.0052 | 0.003 | 0.0044 | 0.0011 | 0.0035 | 0.0012 | 0.0033 | 0.0027 | 0.0012 | 0.0024 | 0.0014 | 0.0016 |
Endosome | 0.0219 | 0.0017 | 0.0144 | 0.0151 | 0.0375 | 0.0092 | 0.0374 | 0.0017 | 0.0135 | 0.013 | 0.0721 | 0.0208 | 0.0289 | 0.0009 | 0.0044 | 0.0116 | 0.0013 | 0.0038 |
Golgi | 0.0052 | 0.0001 | 0.0059 | 0.0048 | 0.0053 | 0.002 | 0.0097 | 0.0004 | 0.0023 | 0.0095 | 0.0265 | 0.0051 | 0.008 | 0.0001 | 0.0041 | 0.0132 | 0 | 0.0014 |
Lipid Particles | 0.0131 | 0.0002 | 0.0112 | 0.025 | 0.0132 | 0.0006 | 0.0098 | 0.0002 | 0.0017 | 0.0012 | 0.0069 | 0.0079 | 0.0146 | 0.0001 | 0.0044 | 0.0138 | 0 | 0.0008 |
Mitochondria | 0.0028 | 0.0001 | 0.0076 | 0.018 | 0.0022 | 0.0004 | 0.0047 | 0.005 | 0.0008 | 0.0047 | 0.1152 | 0.0086 | 0.0153 | 0.0001 | 0.0018 | 0.031 | 0.0001 | 0.0009 |
None | 0.419 | 0.3706 | 0.3335 | 0.3461 | 0.3059 | 0.3066 | 0.2949 | 0.2799 | 0.2834 | 0.0814 | 0.2754 | 0.2065 | 0.417 | 0.3712 | 0.2957 | 0.2097 | 0.2305 | 0.2839 |
Nuclear Periphery | 0.0148 | 0.0006 | 0.0088 | 0.0025 | 0.0035 | 0.0022 | 0.0047 | 0.0007 | 0.0048 | 0.0005 | 0.0084 | 0.0006 | 0.0039 | 0.0006 | 0.0005 | 0.0011 | 0.0004 | 0.0004 |
Nucleolus | 0.0006 | 0.0001 | 0.0017 | 0.0004 | 0.0006 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0 | 0.0005 | 0 | 0.0008 |
Nucleus | 0.0174 | 0.0015 | 0.0049 | 0.0029 | 0.0018 | 0.0033 | 0.0037 | 0.0015 | 0.0088 | 0.0013 | 0.0018 | 0.0022 | 0.002 | 0.0013 | 0.0012 | 0.004 | 0.0012 | 0.0013 |
Peroxisomes | 0.01 | 0.0001 | 0.0245 | 0.0139 | 0.0202 | 0.0001 | 0.0086 | 0.0002 | 0.0063 | 0.0049 | 0.0012 | 0.0013 | 0.0065 | 0.0001 | 0.0029 | 0.0157 | 0 | 0.0005 |
Punctate Nuclear | 0.0053 | 0.0005 | 0.0153 | 0.0105 | 0.0176 | 0.0011 | 0.0137 | 0.0004 | 0.0022 | 0.0007 | 0.0014 | 0.0004 | 0.0038 | 0.0006 | 0.0003 | 0.0016 | 0.0002 | 0.0007 |
Vacuole | 0.0019 | 0.0009 | 0.002 | 0.0019 | 0.001 | 0.0006 | 0.0082 | 0.001 | 0.0013 | 0.001 | 0.005 | 0.0018 | 0.0108 | 0.0004 | 0.0007 | 0.0009 | 0.0003 | 0.0009 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0011 | 0.0005 | 0.0005 | 0.0002 | 0.0014 | 0.0001 | 0.0004 | 0.0003 | 0.0033 | 0.0008 | 0.0013 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 58.9425 | 50.5834 | 43.7909 | 63.9692 | 73.3647 | 108.3942 | 59.669 | 58.9705 | 71.3244 | 62.6736 |
Translational Efficiency | 1.8686 | 2.0284 | 2.3206 | 1.9051 | 1.6488 | 1.2505 | 2.0865 | 1.823 | 1.6405 | 1.6468 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |