Standard name
Description Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.08
Bud 0 0 0 0 0 0.1 0.07 0.1 0.06 0.09 0.1 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.1 0.07 0.06 0.05 0.08 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.15 0 0 0 0.12 0 0 0 0 0 0 0 0.16 0.19 0.11 0.13 0.16 0.14 0.13 0.12 0.06
Golgi 0.6 0.35 0.06 0 0 0 0 0 0 0 0 0 0.39 0.47 0.24 0.73 0.7 0.53 0.68 0.72 0.76
Mitochondria 0.33 0.82 0.93 0.89 0.8 0.87 0.89 0.84 0.96 0.91 0.86 0.91 0.63 0.62 0.79 0 0 0.08 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.09 0 0 0.05 0 0.06 0.07 0 0.05 0 0 0 0 0.15 0 0 0.06
SpindlePole 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.08 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 3 3 1 2 2 1 1 1 3 0 1 7 2 7 6 1 5 13 29 31
Bud 2 2 4 4 7 21 18 26 10 15 7 7 7 7 8 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 6 3 2 4 3 0 0 0
Cell Periphery 0 0 0 0 1 0 0 2 1 2 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 11 1 4 11 14 13 12 20 5 8 3 10 7 12 11 2 0 0 2 2 0
Endoplasmic Reticulum 3 4 6 4 4 5 4 0 2 3 0 1 4 2 4 1 0 0 1 1 2
Endosome 35 1 1 1 25 4 10 6 1 0 1 4 43 65 42 37 13 15 28 51 23
Golgi 143 23 6 2 9 4 4 0 0 0 0 2 103 166 89 210 60 59 143 299 297
Mitochondria 78 54 100 101 167 188 229 214 162 154 59 140 167 217 296 8 2 9 10 7 5
Nucleus 2 0 0 2 1 2 2 3 0 3 1 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 1 5 2 0 1 1 5 2 4 4 0 2 0 2 4 1 0 0 0 0 0
Nucleolus 2 0 3 0 4 5 11 7 2 6 1 5 4 15 10 0 0 0 0 0 0
Peroxisomes 3 0 1 2 18 6 5 13 6 10 5 2 13 9 7 9 2 16 7 13 23
SpindlePole 12 0 1 0 9 3 3 13 1 7 0 4 4 3 3 1 0 0 0 0 0
Vac/Vac Membrane 33 5 7 2 5 5 8 9 2 1 3 4 16 15 24 2 1 2 2 2 0
Unique Cell Count 240 66 107 113 208 217 257 256 168 170 69 154 267 351 376 289 86 112 211 414 390
Labelled Cell Count 339 98 138 130 267 259 312 322 200 218 84 186 377 515 506 289 86 112 211 414 390


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 8.5 5.8 5.7 6.2 6.0 5.6 5.6 5.2 5.1 4.7 4.9 9.8 9.6 8.4 10.0 10.1 10.9
Std Deviation (1e-4) 1.6 1.9 1.0 0.9 1.2 1.4 1.2 1.3 1.4 1.1 0.8 1.0 1.4 1.4 1.4 1.2 1.6 1.6
Intensity Change (Log2) -0.03 0.08 0.04 -0.05 -0.07 -0.18 -0.21 -0.31 -0.27 0.74 0.71 0.52 0.78 0.78 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 1.9 1.2 2.0 0.8 1.6 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.4 0 0 0 0 0 0 4.1 4.5 3.3
Golgi 0 -0.5 0 0 0 0 0 0 6.3 7.8 4.1
Mitochondria -1.1 -3.1 -1.8 -1.3 -2.5 1.1 -0.8 0 -6.0 -6.2 -3.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 1.1 0.9 0 1.4 0 0 1.0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 -0.2 -1.0 -0.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dop1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available