| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 14 | 3 | 3 | 1 | 2 | 2 | 1 | 1 | 1 | 3 | 0 | 1 | 7 | 2 | 7 | – | – | – | 6 | 1 | 5 | 13 | 29 | 31 |
| Bud | 2 | 2 | 4 | 4 | 7 | 21 | 18 | 26 | 10 | 15 | 7 | 7 | 7 | 7 | 8 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 2 | 4 | 3 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 11 | 1 | 4 | 11 | 14 | 13 | 12 | 20 | 5 | 8 | 3 | 10 | 7 | 12 | 11 | – | – | – | 2 | 0 | 0 | 2 | 2 | 0 |
| Endoplasmic Reticulum | 3 | 4 | 6 | 4 | 4 | 5 | 4 | 0 | 2 | 3 | 0 | 1 | 4 | 2 | 4 | – | – | – | 1 | 0 | 0 | 1 | 1 | 2 |
| Endosome | 35 | 1 | 1 | 1 | 25 | 4 | 10 | 6 | 1 | 0 | 1 | 4 | 43 | 65 | 42 | – | – | – | 37 | 13 | 15 | 28 | 51 | 23 |
| Golgi | 143 | 23 | 6 | 2 | 9 | 4 | 4 | 0 | 0 | 0 | 0 | 2 | 103 | 166 | 89 | – | – | – | 210 | 60 | 59 | 143 | 299 | 297 |
| Mitochondria | 78 | 54 | 100 | 101 | 167 | 188 | 229 | 214 | 162 | 154 | 59 | 140 | 167 | 217 | 296 | – | – | – | 8 | 2 | 9 | 10 | 7 | 5 |
| Nucleus | 2 | 0 | 0 | 2 | 1 | 2 | 2 | 3 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 1 | 5 | 2 | 0 | 1 | 1 | 5 | 2 | 4 | 4 | 0 | 2 | 0 | 2 | 4 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 2 | 0 | 3 | 0 | 4 | 5 | 11 | 7 | 2 | 6 | 1 | 5 | 4 | 15 | 10 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 3 | 0 | 1 | 2 | 18 | 6 | 5 | 13 | 6 | 10 | 5 | 2 | 13 | 9 | 7 | – | – | – | 9 | 2 | 16 | 7 | 13 | 23 |
| SpindlePole | 12 | 0 | 1 | 0 | 9 | 3 | 3 | 13 | 1 | 7 | 0 | 4 | 4 | 3 | 3 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | 33 | 5 | 7 | 2 | 5 | 5 | 8 | 9 | 2 | 1 | 3 | 4 | 16 | 15 | 24 | – | – | – | 2 | 1 | 2 | 2 | 2 | 0 |
| Unique Cell Count | 240 | 66 | 107 | 113 | 208 | 217 | 257 | 256 | 168 | 170 | 69 | 154 | 267 | 351 | 376 | 289 | 86 | 112 | 211 | 414 | 390 | |||
| Labelled Cell Count | 339 | 98 | 138 | 130 | 267 | 259 | 312 | 322 | 200 | 218 | 84 | 186 | 377 | 515 | 506 | 289 | 86 | 112 | 211 | 414 | 390 | |||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 11.4 | 8.5 | 5.8 | 5.7 | 6.2 | 6.0 | 5.6 | 5.6 | 5.2 | 5.1 | 4.7 | 4.9 | 9.8 | 9.6 | 8.4 | – | – | – | 10.0 | 10.1 | 10.9 |
| Std Deviation (1e-4) | 1.6 | 1.9 | 1.0 | 0.9 | 1.2 | 1.4 | 1.2 | 1.3 | 1.4 | 1.1 | 0.8 | 1.0 | 1.4 | 1.4 | 1.4 | – | – | – | 1.2 | 1.6 | 1.6 |
| Intensity Change (Log2) | – | – | – | -0.03 | 0.08 | 0.04 | -0.05 | -0.07 | -0.18 | -0.21 | -0.31 | -0.27 | 0.74 | 0.71 | 0.52 | – | – | – | 0.78 | 0.78 | 0.9 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | 0 | 0 | 1.9 | 1.2 | 2.0 | 0.8 | 1.6 | – | 0 | 0 | 0 | 0 | – | – | – |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Endosome | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | – | 0 | 4.1 | 4.5 | 3.3 | – | – | – |
| Golgi | 0 | -0.5 | 0 | 0 | 0 | 0 | 0 | – | 0 | 6.3 | 7.8 | 4.1 | – | – | – |
| Mitochondria | -1.1 | -3.1 | -1.8 | -1.3 | -2.5 | 1.1 | -0.8 | – | 0 | -6.0 | -6.2 | -3.5 | – | – | – |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Peroxisomes | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Cytoplasm | 1.8 | 1.1 | 0.9 | 0 | 1.4 | 0 | 0 | – | 1.0 | 0 | 0 | 0 | – | – | – |
| Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | – |
| Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | -0.2 | -1.0 | -0.1 | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 25.5448 | 20.9003 | 24.6994 | 34.658 | 24.1382 | 23.1507 | 28.372 | 31.1669 | 30.0905 | 37.0827 |
| Translational Efficiency | 0.6136 | 0.5718 | 0.5327 | 0.5004 | 0.5567 | 0.603 | 0.5446 | 0.388 | 0.4757 | 0.4372 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies |
| Localization | |
| Cell Percentages | Golgi (10%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Dop1
Dop1
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Dop1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
