Standard name
Human Ortholog
Description Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.08
Bud 0 0 0 0 0 0.1 0.07 0.1 0.06 0.09 0.1 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.1 0.07 0.06 0.05 0.08 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.15 0 0 0 0.12 0 0 0 0 0 0 0 0.16 0.19 0.11 0.13 0.16 0.14 0.13 0.12 0.06
Golgi 0.6 0.35 0.06 0 0 0 0 0 0 0 0 0 0.39 0.47 0.24 0.73 0.7 0.53 0.68 0.72 0.76
Mitochondria 0.33 0.82 0.93 0.89 0.8 0.87 0.89 0.84 0.96 0.91 0.86 0.91 0.63 0.62 0.79 0 0 0.08 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.09 0 0 0.05 0 0.06 0.07 0 0.05 0 0 0 0 0.15 0 0 0.06
SpindlePole 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.08 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 3 3 1 2 2 1 1 1 3 0 1 7 2 7 6 1 5 13 29 31
Bud 2 2 4 4 7 21 18 26 10 15 7 7 7 7 8 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 6 3 2 4 3 0 0 0
Cell Periphery 0 0 0 0 1 0 0 2 1 2 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 11 1 4 11 14 13 12 20 5 8 3 10 7 12 11 2 0 0 2 2 0
Endoplasmic Reticulum 3 4 6 4 4 5 4 0 2 3 0 1 4 2 4 1 0 0 1 1 2
Endosome 35 1 1 1 25 4 10 6 1 0 1 4 43 65 42 37 13 15 28 51 23
Golgi 143 23 6 2 9 4 4 0 0 0 0 2 103 166 89 210 60 59 143 299 297
Mitochondria 78 54 100 101 167 188 229 214 162 154 59 140 167 217 296 8 2 9 10 7 5
Nucleus 2 0 0 2 1 2 2 3 0 3 1 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 1 5 2 0 1 1 5 2 4 4 0 2 0 2 4 1 0 0 0 0 0
Nucleolus 2 0 3 0 4 5 11 7 2 6 1 5 4 15 10 0 0 0 0 0 0
Peroxisomes 3 0 1 2 18 6 5 13 6 10 5 2 13 9 7 9 2 16 7 13 23
SpindlePole 12 0 1 0 9 3 3 13 1 7 0 4 4 3 3 1 0 0 0 0 0
Vac/Vac Membrane 33 5 7 2 5 5 8 9 2 1 3 4 16 15 24 2 1 2 2 2 0
Unique Cell Count 240 66 107 113 208 217 257 256 168 170 69 154 267 351 376 289 86 112 211 414 390
Labelled Cell Count 339 98 138 130 267 259 312 322 200 218 84 186 377 515 506 289 86 112 211 414 390


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.4 8.5 5.8 5.7 6.2 6.0 5.6 5.6 5.2 5.1 4.7 4.9 9.8 9.6 8.4 10.0 10.1 10.9
Std Deviation (1e-4) 1.6 1.9 1.0 0.9 1.2 1.4 1.2 1.3 1.4 1.1 0.8 1.0 1.4 1.4 1.4 1.2 1.6 1.6
Intensity Change (Log2) -0.03 0.08 0.04 -0.05 -0.07 -0.18 -0.21 -0.31 -0.27 0.74 0.71 0.52 0.78 0.78 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 1.9 1.2 2.0 0.8 1.6 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.4 0 0 0 0 0 0 4.1 4.5 3.3
Golgi 0 -0.5 0 0 0 0 0 0 6.3 7.8 4.1
Mitochondria -1.1 -3.1 -1.8 -1.3 -2.5 1.1 -0.8 0 -6.0 -6.2 -3.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 1.1 0.9 0 1.4 0 0 1.0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 -0.2 -1.0 -0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.5448 20.9003 24.6994 34.658 24.1382 23.1507 28.372 31.1669 30.0905 37.0827
Translational Efficiency 0.6136 0.5718 0.5327 0.5004 0.5567 0.603 0.5446 0.388 0.4757 0.4372

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
475 1537 1139 1497 1615 2047 1033 2152 2090 3584 2172 3649

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 950.59 1028.41 1370.51 1232.78 837.65 1020.32 1193.80 1300.14 863.32 1023.79 1286.47 1272.51
Standard Deviation 177.82 155.59 186.04 185.98 128.35 145.57 165.48 207.64 148.85 150.00 197.39 201.78
Intensity Change Log 2 0.113520 0.527818 0.375020 0.284602 0.511142 0.634248 0.196189 0.520030 0.502261

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.022020 0.003813 0.006020 0.001205 0.004279 0.003091 0.002516 0.009068 0.008311 0.003401 0.004354 0.005842
Bud Neck 0.000339 0.000131 0.001090 0.000693 0.000204 0.000063 0.000652 0.000210 0.000235 0.000092 0.000882 0.000408
Bud Site 0.001574 0.000641 0.002460 0.002335 0.000500 0.000410 0.001427 0.002454 0.000744 0.000509 0.001969 0.002405
Cell Periphery 0.000224 0.000037 0.000071 0.000043 0.000067 0.000045 0.000054 0.000080 0.000103 0.000041 0.000063 0.000065
Cytoplasm 0.001035 0.000392 0.000548 0.000981 0.000350 0.000269 0.000483 0.001436 0.000506 0.000322 0.000517 0.001249
Cytoplasmic Foci 0.129114 0.273723* 0.169549 0.217737* 0.209237* 0.266643* 0.324518* 0.136665 0.191027* 0.269680* 0.243252* 0.169925
Eisosomes 0.000214 0.000044 0.000036 0.000017 0.000068 0.000039 0.000029 0.000045 0.000101 0.000041 0.000032 0.000033
Endoplasmic Reticulum 0.002500 0.000228 0.001188 0.000392 0.000431 0.000170 0.000509 0.000284 0.000901 0.000195 0.000865 0.000328
Endosome 0.175753 0.171992 0.095173 0.318668* 0.229677* 0.204707* 0.228517* 0.170957 0.217422* 0.190677 0.158591 0.231556*
Golgi 0.578953* 0.513775* 0.651155* 0.355640* 0.492382* 0.488035* 0.337395* 0.633458* 0.512057* 0.499074* 0.501931* 0.519483*
Lipid Particles 0.018787 0.015683 0.020963 0.015636 0.026673 0.011429 0.047201 0.005411 0.024880 0.013254 0.033442 0.009606
Mitochondria 0.045443 0.008633 0.000807 0.003564 0.022698 0.013217 0.006008 0.004522 0.027868 0.011251 0.003281 0.004129
Mitotic Spindle 0.000884 0.001435 0.009051 0.019483 0.000723 0.001469 0.013508 0.003122 0.000760 0.001454 0.011171 0.009834
None 0.000065 0.000012 0.000021 0.000015 0.000047 0.000023 0.000017 0.000024 0.000051 0.000018 0.000019 0.000021
Nuclear Periphery 0.000502 0.000038 0.000937 0.000484 0.000039 0.000041 0.000535 0.000165 0.000144 0.000040 0.000746 0.000296
Nuclear Periphery Foci 0.001699 0.000156 0.004796 0.004608 0.000462 0.000204 0.003750 0.000690 0.000743 0.000183 0.004299 0.002297
Nucleolus 0.000017 0.000006 0.000013 0.000058 0.000155 0.000015 0.000044 0.000007 0.000124 0.000011 0.000028 0.000028
Nucleus 0.002221 0.000010 0.000023 0.000053 0.000154 0.000016 0.000057 0.000341 0.000624 0.000013 0.000039 0.000223
Peroxisomes 0.002344 0.003399 0.001590 0.003838 0.005639 0.004061 0.003759 0.002210 0.004890 0.003777 0.002622 0.002878
Vacuole 0.007455 0.002835 0.023686 0.040159 0.002550 0.002756 0.020764 0.018181 0.003665 0.002790 0.022296 0.027198
Vacuole Periphery 0.008859 0.003016 0.010824 0.014391 0.003662 0.003298 0.008258 0.010669 0.004843 0.003177 0.009604 0.012196

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.71 4.06 5.45 3.56 4.65 1.15 1.75 -3.04 -4.10 -4.56 4.15 3.18 1.82 -2.68 -1.50
Bud Neck 3.61 -3.26 -3.17 -5.00 1.36 3.10 -4.04 -0.34 -3.64 3.86 3.67 -4.87 -3.10 -6.06 3.19
Bud Site 2.45 -1.66 -2.11 -5.12 -0.02 1.06 -5.24 -4.33 -4.53 -2.23 1.82 -4.74 -5.67 -6.37 -1.38
Cell Periphery 8.47 6.73 8.17 -1.10 3.75 1.53 1.42 -1.24 -2.22 -2.22 5.81 4.67 3.48 -2.45 -0.46
Cytoplasm 2.09 1.57 -0.22 -2.79 -2.17 2.64 -2.29 -3.04 -3.24 -2.69 2.38 -0.17 -3.29 -4.08 -3.31
Cytoplasmic Foci -21.24 -5.81 -12.50 8.47 -7.15 -11.06 -15.51 15.77 26.89 26.14* -18.70 -9.91 5.14 25.02 14.38
Eisosomes 7.80 8.17 8.97 5.88 4.07 7.39 9.58 4.50 -1.37 -3.51 10.10 11.42 10.66 2.34 -0.27
Endoplasmic Reticulum 5.91 3.25 5.12 -1.08 4.11 3.92 -0.78 1.72 -2.09 2.34 6.85 0.28 4.65 -1.93 5.25
Endosome 0.46 10.24 -15.02 -20.55 -33.18* 4.45 0.16 10.47 7.06 8.55 5.79 11.31 -2.70 -9.59 -14.95
Golgi 4.92 -4.96 15.83 17.30 26.94* 0.61 15.96 -18.33 -20.26 -30.47* 2.09 1.25 -0.92 -3.20 -2.05
Lipid Particles 0.82 -0.52 0.84 -0.01 1.88 5.37 -4.28 7.97 4.08 10.11 4.93 -2.73 6.77 2.79 9.75
Mitochondria 5.85 7.26 6.77 3.43 -3.96 3.82 6.73 8.42 5.96 1.02 7.09 10.99 10.87 6.80 -1.08
Mitotic Spindle -1.06 -6.20 -8.23 -7.84 -4.14 -1.37 -5.74 -3.60 -2.00 4.47 -1.82 -8.22 -8.93 -7.84 0.69
None 7.82 5.93 7.18 -1.70 1.74 1.78 2.86 1.23 -0.55 -1.39 3.41 3.78 2.73 -0.77 -0.82
Nuclear Periphery 1.20 -1.05 0.03 -8.78 2.64 -0.13 -4.46 -2.08 -2.02 2.93 1.19 -4.52 -1.59 -6.12 4.13
Nuclear Periphery Foci 1.12 -1.87 -1.97 -7.05 0.12 2.00 -4.50 -1.03 -2.43 4.09 1.71 -5.18 -3.66 -7.38 2.95
Nucleolus 1.60 0.45 -2.16 -2.83 -2.38 1.31 1.03 1.38 0.59 1.79 1.37 1.16 1.14 -1.85 -0.15
Nucleus 1.19 1.18 1.17 -2.84 -2.02 1.29 0.89 -0.93 -1.39 -1.27 1.42 1.36 0.69 -1.48 -1.35
Peroxisomes -1.60 1.41 -2.37 -0.69 -3.81 1.77 2.33 4.81 2.81 2.90 1.69 3.83 3.43 1.82 -0.72
Vacuole 2.69 -6.65 -12.43 -18.18 -6.17 -0.64 -10.80 -12.47 -12.28 1.18 1.89 -14.43 -19.69 -21.48 -3.03
Vacuole Periphery 4.53 -1.31 -3.46 -10.89 -2.68 0.86 -5.36 -9.41 -10.35 -2.34 3.81 -6.77 -10.73 -15.01 -3.15