Standard name
Description Highly-acidic RWD domain-containing cytoplasmic protein; forms a highly conserved complex with Rbg2p that is responsible for efficient cell growth under amino acid starvation and binds translational activator Gcn1p in dose-dependent manner according to stress level; associates with translating ribosomes; intrinsically unstructured protein whose stability is enhanced upon binding Rbg2p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0 0 0 0.07 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0.06 0 0.06 0 0.08 0.08 0.08 – – – 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.97 0.95 0.94 0.88 0.84 0.8 0.72 0.71 0.74 0.68 – – – 0.96 0.95 0.98 0.95 0.96 0.94 0.55 0.75 0.72
Endoplasmic Reticulum 0 0 0 0 0 0.09 0.05 0.11 0.1 0.12 0.11 0.08 – – – 0 0 0 0 0 0 0.11 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.09 0 0.2 0.14 0.12 0.18 – – – 0 0 0 0 0 0 0.11 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.11 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.07
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.06 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 9 8 25 9 15 11 31 – – – 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 1 4 2 0 1 0 2 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 7 4 11 11 24 8 30 12 29 27 34 – – – 3 4 2 0 0 0 0 0 0
Cytoplasm 163 138 229 257 323 364 326 383 231 263 245 287 – – – 120 113 41 203 140 238 4 11 25
Endoplasmic Reticulum 4 4 6 8 12 36 21 54 31 44 36 32 – – – 4 2 1 1 0 8 1 0 0
Endosome 0 0 0 0 0 0 0 1 0 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 2 0 9 1 1 0 2 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 1 2 8 35 20 63 51 38 74 – – – 3 0 1 0 0 1 0 0 0
Nucleus 0 0 0 0 1 0 0 1 5 1 4 0 – – – 2 3 1 2 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 3 3 3 5 16 19 8 16 5 25 – – – 0 1 0 1 4 1 0 0 0
Unique Cell Count 169 143 236 271 343 413 389 481 320 368 329 421 125 119 42 214 146 254 9 15 35
Labelled Cell Count 171 149 242 281 354 449 419 544 360 421 367 489 132 123 46 214 146 254 9 15 35


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.7 20.5 19.7 18.2 16.6 13.5 9.5 12.1 10.5 11.0 10.7 10.7 – – – 18.3 23.2 24.0 – – 20.3
Std Deviation (1e-4) 4.2 3.7 2.7 2.6 2.1 1.9 2.2 1.9 1.8 1.8 1.9 1.8 – – – 5.6 4.6 5.5 – – 8.2
Intensity Change (Log2) – – – -0.12 -0.25 -0.54 -1.05 -0.71 -0.91 -0.84 -0.88 -0.89 – – – -0.11 0.24 0.28 – – 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.6 0 3.1 0 4.3 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 1.6 1.1 2.5 0 2.7 1.4 3.3 3.4 3.4 – – – 0 0 0
Cytoplasm -1.2 -1.6 -3.9 -5.1 -6.2 -7.7 -7.9 -7.2 -8.6 – – – -0.5 -1.0 0.2
Endoplasmic Reticulum 0.3 0.7 3.1 1.7 3.9 3.3 4.1 3.8 2.7 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 4.7 3.2 7.2 6.0 5.4 6.8 – – – 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 2.0 2.0 0 2.1 0 2.8 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.8883 17.3432 14.9516 15.1228 10.5696 15.6763 18.27 23.5407 20.631 21.4848 20.5168 20.6512 12.8403 20.7838 18.12 16.3363 16.7279 18.2353
Actin 0.0535 0.0006 0.0257 0.0008 0.0171 0.0006 0.0138 0.0012 0.0158 0.0004 0.0002 0.0009 0.0657 0.0004 0.0098 0.0407 0.0034 0.003
Bud 0.0011 0.0005 0.0011 0.0016 0.0043 0.0003 0.0005 0.0119 0.0007 0.0006 0.0002 0.0004 0.0008 0.0002 0.0006 0.0008 0.0002 0.0002
Bud Neck 0.0012 0.0004 0.002 0.0006 0.0019 0.0016 0.0005 0.0002 0.0004 0.0004 0.0003 0.0008 0.0033 0.0003 0.0004 0.0007 0.001 0.001
Bud Periphery 0.0011 0.0002 0.0007 0.0005 0.0059 0.0001 0.0008 0.0011 0.0003 0.0001 0 0.0002 0.0008 0 0.0004 0.0006 0.0001 0.0001
Bud Site 0.0019 0.0005 0.0039 0.0004 0.001 0 0.0006 0.001 0.0011 0.0001 0 0.0003 0.0066 0.0003 0.0009 0.001 0.0001 0.0001
Cell Periphery 0.0007 0.0003 0.001 0.0003 0.0013 0.0001 0.0008 0.0002 0.0001 0 0 0.0001 0.0011 0.0001 0.0003 0.0003 0.0001 0.0001
Cytoplasm 0.7003 0.9531 0.8451 0.8257 0.6224 0.8966 0.8962 0.9661 0.9182 0.9669 0.9486 0.9228 0.6464 0.9769 0.8944 0.807 0.8504 0.9127
Cytoplasmic Foci 0.0183 0.0034 0.0123 0.0157 0.0197 0.01 0.0074 0.0045 0.0099 0.0068 0.0136 0.0143 0.0304 0.0022 0.0057 0.0117 0.025 0.0057
Eisosomes 0.0007 0 0.0002 0 0.0005 0 0.0001 0 0.0001 0 0 0 0.0008 0 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0601 0.0129 0.0207 0.0047 0.0091 0.0455 0.0194 0.0035 0.0058 0.0018 0.006 0.0082 0.0921 0.0118 0.0266 0.0095 0.0104 0.0461
Endosome 0.0493 0.0073 0.0386 0.0816 0.0727 0.0304 0.0198 0.0046 0.0211 0.0109 0.0249 0.0292 0.0452 0.0034 0.0162 0.0333 0.0424 0.0164
Golgi 0.0218 0.0011 0.0076 0.0027 0.0314 0.0032 0.004 0.0013 0.0058 0.0005 0.0013 0.0028 0.0126 0.0006 0.0022 0.0144 0.0023 0.002
Lipid Particles 0.0197 0.0001 0.0044 0.0002 0.0571 0 0.0004 0 0.0014 0 0 0.0001 0.0139 0 0.0065 0.0057 0.0038 0.0003
Mitochondria 0.0182 0.0005 0.0146 0.0011 0.1059 0.0031 0.0075 0.0008 0.0037 0.0003 0.0005 0.0018 0.0053 0.0002 0.0064 0.0038 0.0007 0.0026
None 0.0049 0.0002 0.0006 0.0004 0.0095 0.0004 0.0013 0.0003 0.0007 0.0004 0.0003 0.0005 0.012 0.0002 0.0008 0.0009 0.0013 0.0004
Nuclear Periphery 0.0148 0.002 0.0026 0.0025 0.0047 0.0016 0.0096 0.0006 0.0007 0.0009 0.0009 0.0051 0.0143 0.0006 0.01 0.0036 0.0024 0.0033
Nucleolus 0.0004 0 0.0003 0.0001 0.0009 0 0.0001 0 0 0 0 0.0001 0.0024 0 0.0001 0.0001 0.0001 0
Nucleus 0.0036 0.0011 0.0015 0.0016 0.0026 0.001 0.0022 0.0008 0.0007 0.0011 0.0005 0.0038 0.006 0.0006 0.0031 0.0019 0.001 0.0012
Peroxisomes 0.0094 0 0.0012 0.0001 0.0081 0.0001 0.0002 0.0001 0.0024 0 0.0001 0.0002 0.0077 0 0.0049 0.0025 0.0174 0.0001
Punctate Nuclear 0.0049 0 0.0018 0.0001 0.0008 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0003 0.009 0 0.0004 0.0008 0.0197 0.0001
Vacuole 0.0098 0.014 0.0103 0.0424 0.0128 0.0034 0.0124 0.0015 0.0093 0.0068 0.0017 0.0058 0.0195 0.002 0.0085 0.0546 0.0149 0.0032
Vacuole Periphery 0.0043 0.002 0.0037 0.017 0.0105 0.002 0.0021 0.0003 0.0014 0.0018 0.0008 0.0023 0.0042 0.0002 0.0016 0.0058 0.0033 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.3085 56.8588 61.4531 57.7644 54.6788 35.9306 57.8023 56.5156 54.3782 54.9869
Translational Efficiency 1.9703 1.8286 1.4831 1.6189 1.6419 2.4438 1.4286 1.326 1.3307 1.5148

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘