Standard name | |
---|---|
Human Ortholog | |
Description | RNA polymerase I subunit A14 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | 1 | 0 | 3 | 11 | 6 | 1 | 5 | 4 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 7 | 2 | 1 | 2 | 3 |
Bud Neck | 7 | 12 | 15 | 0 | 0 | 2 | 14 | 9 | 2 | 16 | 5 | 14 | 9 | 1 | 3 | 2 | 0 | 1 | 2 | 5 | 1 | 0 | 3 | 1 |
Bud Site | 0 | 3 | 7 | 6 | 6 | 8 | 24 | 16 | 18 | 25 | 24 | 45 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 4 | 3 | 1 | 9 | 4 | 16 | 11 | 1 | 6 | 8 | 7 | 10 | 38 | 43 | 10 | 6 | 14 | 1 | 0 | 0 | 2 | 2 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 1 | 3 | 1 | 1 | 19 | 11 | 6 | 8 | 2 | 3 | 1 | 1 | 1 | 7 | 17 | 2 | 1 | 0 | 3 | 4 | 4 | 3 | 0 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 |
Mitochondria | 13 | 12 | 15 | 48 | 27 | 54 | 56 | 44 | 58 | 56 | 55 | 73 | 7 | 15 | 8 | 17 | 25 | 19 | 1 | 2 | 0 | 0 | 2 | 1 |
Nucleus | 61 | 35 | 23 | 22 | 17 | 16 | 22 | 26 | 16 | 18 | 35 | 38 | 9 | 25 | 18 | 10 | 11 | 10 | 41 | 30 | 8 | 5 | 7 | 7 |
Nuclear Periphery | 4 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 389 | 389 | 168 | 177 | 281 | 334 | 430 | 526 | 301 | 351 | 370 | 470 | 190 | 214 | 211 | 181 | 160 | 95 | 344 | 347 | 115 | 119 | 173 | 156 |
Peroxisomes | 2 | 9 | 0 | 2 | 5 | 20 | 30 | 39 | 11 | 18 | 25 | 32 | 5 | 8 | 8 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
SpindlePole | 40 | 44 | 40 | 21 | 74 | 121 | 213 | 216 | 112 | 166 | 155 | 205 | 30 | 34 | 47 | 17 | 11 | 2 | 36 | 40 | 44 | 19 | 51 | 50 |
Vac/Vac Membrane | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 3 | 0 | 0 | 1 | 3 | 2 | 1 | 2 | 2 | 1 | 4 | 1 | 3 | 5 | 7 |
Unique Cell Count | 432 | 428 | 183 | 197 | 316 | 371 | 525 | 609 | 333 | 425 | 433 | 580 | 212 | 277 | 292 | 201 | 178 | 120 | 446 | 448 | 184 | 166 | 255 | 256 |
Labelled Cell Count | 522 | 517 | 276 | 279 | 439 | 573 | 823 | 904 | 522 | 667 | 684 | 889 | 262 | 347 | 364 | 243 | 218 | 145 | 446 | 448 | 184 | 166 | 255 | 256 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.5 | 13.4 | 7.0 | 9.3 | 9.2 | 7.7 | 7.2 | 8.1 | 7.0 | 7.8 | 7.5 | 7.7 | 16.1 | 14.0 | 14.3 | 21.4 | 21.0 | 17.1 | 14.4 | 16.3 | 16.2 |
Std Deviation (1e-4) | 3.2 | 4.6 | 2.1 | 2.9 | 2.7 | 2.2 | 2.5 | 2.7 | 2.1 | 2.8 | 2.6 | 2.7 | 7.5 | 6.2 | 8.4 | 9.9 | 9.0 | 6.1 | 5.8 | 5.5 | 4.6 |
Intensity Change (Log2) | – | – | – | 0.42 | 0.4 | 0.15 | 0.04 | 0.22 | 0.01 | 0.16 | 0.1 | 0.14 | 1.21 | 1.01 | 1.04 | 1.62 | 1.59 | 1.29 | 1.05 | 1.22 | 1.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -4.1 | -5.2 | -4.9 | -3.3 | -4.6 | -4.6 | -2.3 | -4.5 | -3.6 | -1.6 | -4.5 | -4.0 | -3.4 | -3.9 | -2.8 |
Bud Site | -0.4 | 0 | 0 | 0.4 | -0.8 | 0.8 | 1.0 | 0.9 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 4.5 | 4.7 | 1.8 | 0 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.2 | 0.1 | 2.1 | 1.0 | -0.4 | 2.9 | 1.8 | 1.6 | 1.6 | -2.1 | -1.2 | -2.7 | 0.1 | 1.8 | 2.1 |
Nucleus | -0.4 | -2.8 | -3.6 | -4.0 | -4.1 | -3.2 | -3.8 | -1.7 | -2.6 | -3.0 | -1.2 | -2.4 | -2.7 | -2.1 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.7 | -1.0 | -0.7 | -3.2 | -2.0 | -0.5 | -2.9 | -2.2 | -3.4 | -0.7 | -4.1 | -5.2 | -0.6 | -0.6 | -3.2 |
Peroxisomes | 0 | 0 | 3.2 | 3.3 | 3.5 | 0 | 2.8 | 3.3 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -3.0 | 0.4 | 2.6 | 4.5 | 3.5 | 2.8 | 4.1 | 3.4 | 3.4 | -2.0 | -2.7 | -1.6 | -3.7 | -4.3 | -5.0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.8585 | 16.6073 | 15.2537 | 14.6382 | 11.5195 | 14.5298 | 16.414 | 17.3031 | 15.4892 | 15.1912 | 14.3001 | 15.195 | 19.3376 | 17.9109 | 17.8726 | 17.5784 | 16.1854 | 16.6244 |
Actin | 0.0126 | 0 | 0.0025 | 0 | 0.0166 | 0.0012 | 0.0001 | 0 | 0.0045 | 0 | 0.0006 | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0004 | 0 | 0.0001 | 0 | 0.002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Periphery | 0.0005 | 0 | 0.0001 | 0 | 0.003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0047 | 0.0001 | 0.0006 | 0 | 0.017 | 0.0002 | 0.0006 | 0 | 0.0059 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0074 | 0 | 0.0033 | 0 | 0.0039 | 0.0004 | 0.0011 | 0 | 0.0033 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0184 | 0.0001 | 0.0097 | 0.0003 | 0.0357 | 0.0228 | 0.0063 | 0.0001 | 0.0045 | 0 | 0.0145 | 0.0071 | 0.0018 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0002 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0044 | 0 | 0.0011 | 0 | 0.0016 | 0.0004 | 0.0003 | 0 | 0.0014 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0234 | 0 | 0.0063 | 0 | 0.0575 | 0.001 | 0.0011 | 0 | 0.0027 | 0 | 0.0014 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0051 | 0 | 0.0025 | 0 | 0.0224 | 0.0008 | 0.0002 | 0 | 0.0014 | 0 | 0.0008 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0151 | 0.0002 | 0.0068 | 0.0007 | 0.0386 | 0.0115 | 0.0035 | 0.0001 | 0.0037 | 0 | 0.015 | 0.0023 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0024 | 0.0003 |
Mitochondria | 0.0041 | 0.0005 | 0.0018 | 0.0004 | 0.0474 | 0.007 | 0.0017 | 0 | 0.0028 | 0 | 0.0101 | 0.0017 | 0.0004 | 0.0001 | 0 | 0.0041 | 0.006 | 0.0001 |
None | 0.0044 | 0 | 0.0022 | 0 | 0.0024 | 0.0022 | 0.006 | 0 | 0.001 | 0 | 0.0003 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0275 | 0 | 0.0009 | 0 | 0.003 | 0.0095 | 0.0031 | 0 | 0.0004 | 0 | 0.0002 | 0.0014 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Nucleolus | 0.7798 | 0.8769 | 0.872 | 0.9361 | 0.6768 | 0.889 | 0.914 | 0.9151 | 0.9059 | 0.9073 | 0.8998 | 0.9269 | 0.9434 | 0.8783 | 0.942 | 0.9371 | 0.8933 | 0.9353 |
Nucleus | 0.0502 | 0.1171 | 0.0723 | 0.0622 | 0.0332 | 0.0446 | 0.0485 | 0.0817 | 0.0564 | 0.0902 | 0.0394 | 0.0546 | 0.0502 | 0.1184 | 0.0552 | 0.0576 | 0.0577 | 0.0571 |
Peroxisomes | 0.0207 | 0 | 0.0009 | 0 | 0.0156 | 0.0004 | 0.0003 | 0 | 0.0024 | 0 | 0.0094 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0.0135 | 0.0001 | 0.0156 | 0.0001 | 0.0135 | 0.0057 | 0.0105 | 0.0026 | 0.0019 | 0.0022 | 0.0077 | 0.0022 | 0.0027 | 0.0024 | 0.0025 | 0.0004 | 0.0386 | 0.0068 |
Vacuole | 0.0036 | 0.0014 | 0.0006 | 0.0001 | 0.0057 | 0.0004 | 0.0013 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Vacuole Periphery | 0.0027 | 0.0034 | 0.0003 | 0.0001 | 0.0025 | 0.0024 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 97.2808 | 78.6242 | 88.3978 | 79.7359 | 77.7507 | 47.9475 | 77.6901 | 59.3457 | 69.5943 | 64.4721 |
Translational Efficiency | 1.8585 | 2.1283 | 1.6322 | 1.8848 | 1.9935 | 3.3971 | 1.7379 | 3.2137 | 2.1871 | 2.0681 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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