Standard name
Description Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.13 0.15 0.08 0.07 0 0.06 – 0.05 0 0 0.06 0 0 0 0.12 0.11 0.12 0.23 0.16 0.16 0.13 0.18 0.14
Bud 0.08 0.05 0 0.05 0 0 0.07 – 0.07 0.08 0.1 0.09 0 0 0 0.05 0.06 0.05 0.07 0.07 0.07 0.13 0.1 0.09
Bud Neck 0 0 0.1 0.06 0.1 0 0.05 – 0 0 0 0 0 0 0 0 0.08 0.05 0.13 0.18 0.19 0.19 0.12 0.08
Bud Site 0.05 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.05
Cytoplasm 0.75 0.8 0.73 0.84 0.82 0.86 0.77 – 0.7 0.66 0.61 0.6 0.92 0.94 0.94 0.73 0.73 0.76 0.35 0.41 0.31 0.28 0.32 0.43
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.09 – 0.28 0.34 0.33 0.25 0 0 0 0 0 0.05 0.08 0.06 0.1 0.12 0.09 0.09
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.15 0.07 0.08 0.06 0.09 – 0.05 0.07 0.06 0.08 0 0 0 0.13 0.11 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 33 57 18 21 12 26 – 13 5 7 15 9 14 4 86 47 48 32 47 64 58 85 55
Bud 10 13 9 12 9 12 32 – 16 19 24 22 16 9 10 33 24 18 9 19 27 55 46 34
Bud Neck 4 8 38 13 29 15 21 – 9 9 5 11 2 7 2 23 36 21 18 53 76 84 54 32
Bud Site 6 11 22 1 2 3 5 – 4 6 7 5 4 1 0 19 8 9 – – – – – –
Cell Periphery 3 2 13 4 7 6 9 – 4 13 6 10 11 24 20 17 19 12 4 8 18 20 28 19
Cytoplasm 94 204 274 185 231 342 330 – 167 165 143 150 494 521 515 499 310 300 50 122 123 122 147 174
Endoplasmic Reticulum 4 11 0 3 2 2 5 – 2 1 0 2 0 4 2 50 19 26 0 1 1 3 9 5
Endosome 0 2 1 0 1 0 2 – 0 0 0 0 4 1 4 6 3 2 5 8 14 9 5 5
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 1 0 0 2 0 0 0 1 1
Mitochondria 3 6 3 2 0 25 38 – 66 84 77 64 3 1 6 29 8 18 11 18 38 54 40 36
Nucleus 0 0 5 0 0 0 4 – 2 3 1 3 0 0 0 2 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 1 0 – 1 0 2 1 0 0 0 0 1 0 0 0 0 0 1 0
Peroxisomes 1 7 0 0 0 3 1 – 0 0 2 4 16 7 14 3 2 1 0 1 2 1 3 1
SpindlePole 7 8 57 15 22 23 38 – 13 18 14 20 18 9 6 91 48 31 4 6 14 8 10 10
Vac/Vac Membrane 0 0 1 3 1 1 2 – 0 1 0 1 0 0 1 4 4 0 0 7 3 14 15 11
Unique Cell Count 125 256 373 221 280 396 428 240 249 236 251 538 556 547 688 427 393 143 301 397 442 462 404
Labelled Cell Count 150 305 480 256 325 445 513 297 324 288 308 578 598 584 863 530 489 143 301 397 442 462 404


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.4 4.9 4.8 5.2 4.1 4.3 – 3.8 3.9 3.8 4.2 4.1 4.3 4.4 5.8 6.1 5.3 3.9 4.1 4.3
Std Deviation (1e-4) 0.5 0.9 0.7 1.4 1.5 1.1 1.1 – 1.0 1.3 1.1 1.0 0.8 1.0 1.8 1.2 1.2 0.9 1.0 1.3 1.7
Intensity Change (Log2) – – – -0.04 0.07 -0.27 -0.2 – -0.36 -0.33 -0.36 -0.24 -0.28 -0.19 -0.15 0.24 0.3 0.11 -0.33 -0.28 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5165 -0.2657 -0.4711 -0.8073 -0.4068 -0.2876 -0.3279 -0.0267 -0.0333 -0.1441 -0.4011 0.0957 -0.4183 -0.3582 -0.4096 -0.253 -0.0525 -0.2027
Actin 0.0182 0.0138 0.0223 0.0024 0.0012 0.0278 0.0268 0.0055 0.019 0.0302 0.0257 0.0091 0.0097 0.0197 0.028 0.0322 0.0131 0.0132
Bud 0.0004 0.0025 0.0045 0.0033 0.0007 0.0005 0.0032 0.0037 0.0161 0.0058 0.0024 0.0013 0.0077 0.0063 0.0096 0.0085 0.002 0.0003
Bud Neck 0.0005 0.002 0.0023 0.0192 0.0004 0.0685 0.0372 0.0042 0.0057 0.0014 0.0054 0.1729 0.0189 0.0095 0.0108 0.001 0.001 0.1571
Bud Periphery 0.0009 0.0034 0.0306 0.033 0.0009 0.0021 0.0041 0.0021 0.0219 0.0095 0.0027 0.0034 0.0096 0.0095 0.0142 0.0245 0.0049 0.0005
Bud Site 0.0014 0.0346 0.0026 0.0143 0.0016 0.0005 0.0305 0.0577 0.027 0.002 0.0012 0.004 0.0252 0.0669 0.0418 0.0028 0.0009 0.0013
Cell Periphery 0.001 0.0013 0.002 0.0024 0.0002 0.0008 0.0021 0.0016 0.0017 0.0024 0.0011 0.0006 0.0011 0.0018 0.0014 0.0008 0.0005 0.0003
Cytoplasm 0.0727 0.0942 0.0793 0.0871 0.1591 0.1226 0.1667 0.3113 0.2852 0.3456 0.407 0.3471 0.1404 0.2045 0.1986 0.3616 0.2399 0.2822
Cytoplasmic Foci 0.037 0.0176 0.0446 0.0082 0.0313 0.0476 0.0593 0.0245 0.0222 0.0239 0.0205 0.0294 0.0734 0.0299 0.0387 0.0121 0.018 0.0215
Eisosomes 0.0009 0.0009 0.001 0.0006 0.0005 0.0006 0.001 0.0003 0.0003 0.0003 0.0003 0.0001 0.0005 0.0005 0.0005 0.0001 0.0005 0.0002
Endoplasmic Reticulum 0.0041 0.0015 0.0041 0.0028 0.0022 0.0009 0.0115 0.0026 0.0039 0.0043 0.0014 0.0019 0.0033 0.0015 0.0042 0.002 0.003 0.001
Endosome 0.0225 0.0076 0.0143 0.0139 0.0096 0.0035 0.0244 0.0027 0.0076 0.0268 0.0024 0.0121 0.0299 0.0045 0.0073 0.0016 0.0967 0.002
Golgi 0.009 0.0032 0.0039 0.0018 0.0011 0.0023 0.0078 0.0008 0.0048 0.0041 0.0008 0.0052 0.0073 0.005 0.0033 0.0009 0.0202 0.001
Lipid Particles 0.0096 0.0081 0.0062 0.0015 0.0052 0.0051 0.013 0.0008 0.0015 0.0012 0.0004 0.0023 0.0153 0.0027 0.0024 0.0003 0.0082 0.0008
Mitochondria 0.0084 0.0022 0.0048 0.0037 0.0008 0.001 0.003 0.0008 0.0067 0.0035 0.0003 0.0019 0.006 0.0043 0.0032 0.0008 0.007 0.002
None 0.7761 0.7962 0.7567 0.7686 0.7617 0.7087 0.5732 0.5741 0.5673 0.5029 0.4981 0.3996 0.6101 0.6171 0.613 0.5461 0.5508 0.5089
Nuclear Periphery 0.0059 0.0003 0.0064 0.0016 0.0014 0.0001 0.0085 0.0011 0.0008 0.0146 0.0023 0.003 0.0043 0.0005 0.0011 0.0004 0.011 0.0031
Nucleolus 0.0009 0.0022 0.0005 0.0021 0.0003 0.0002 0.0018 0.0004 0.0003 0.0017 0.0012 0.0003 0.0041 0.0011 0.0007 0.0002 0.0007 0.0002
Nucleus 0.0093 0.001 0.0015 0.0024 0.0037 0.0003 0.0048 0.002 0.0013 0.0059 0.0076 0.0008 0.005 0.0017 0.0016 0.0009 0.0019 0.0008
Peroxisomes 0.0059 0.0028 0.0065 0.0017 0.0112 0.0054 0.0116 0.0005 0.0027 0.001 0.001 0.0017 0.0121 0.0026 0.0088 0.0014 0.0023 0.0009
Punctate Nuclear 0.009 0.001 0.0015 0.0019 0.0059 0.0008 0.0045 0.0016 0.0013 0.0084 0.0172 0.0016 0.0102 0.0036 0.0021 0.0006 0.0059 0.0017
Vacuole 0.0039 0.0033 0.0031 0.024 0.0009 0.0005 0.0039 0.0016 0.0023 0.0034 0.0008 0.0011 0.0049 0.0065 0.0078 0.0009 0.0073 0.0004
Vacuole Periphery 0.0025 0.0004 0.0013 0.0037 0.0002 0.0002 0.001 0.0002 0.0005 0.0012 0.0001 0.0007 0.001 0.0003 0.0006 0.0001 0.0042 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.5315 10.8842 17.8356 21.8316 14.1417 19.2672 16.2782 17.3311 19.1434 23.8696
Translational Efficiency 0.6784 0.9482 0.6438 0.6189 0.6904 0.5485 0.5853 0.547 0.5887 0.4292

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘