Standard name
Description Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0 0.06 0 0 0 0 0 0 0 0.05 0.12 0.15 0.06 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0.07 0.05 0.07 0.16 0.16 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 – 0.95 0.91 0.89 0.85 0.83 0.82 0.81 0.78 0.82 0.81 0.93 0.85 0.79 0.88 0.86 0.89 0.93 0.94 0.94 0.89 0.79 0.63
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0.15 0.06 0.14 0.19 0.22 0.25 0.24 0.26 0.27 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0
Bud 1 – 0 0 0 0 1 2 1 7 1 4 0 0 0 0 0 0 4 0 4 0 2 4
Bud Neck 0 – 0 0 0 1 3 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 – 0 0 3 6 5 3 7 14 10 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 5 – 5 4 14 8 13 10 7 7 6 5 16 49 58 25 20 10 0 0 0 2 5 4
Endoplasmic Reticulum 0 – 0 0 0 0 2 1 0 0 0 0 1 0 0 5 1 0 0 0 0 1 0 0
Endosome 1 – 0 0 0 0 0 0 1 0 0 0 2 5 6 0 1 0 1 0 0 0 0 2
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1
Mitochondria 2 – 0 2 1 9 32 16 29 80 54 42 0 0 0 3 2 3 1 0 0 0 1 0
Nucleus 348 – 246 86 192 220 364 257 347 400 278 286 304 358 307 340 249 236 347 42 248 169 292 251
Nuclear Periphery 0 – 0 0 2 0 2 2 3 6 0 2 0 0 1 0 1 0 0 0 0 1 1 0
Nucleolus 6 – 8 14 13 36 81 68 106 124 88 94 4 6 15 5 12 11 4 0 5 1 12 24
Peroxisomes 0 – 0 0 0 0 0 0 1 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 3 2 3 0 0 0 1 0 0 0 1 3
Vac/Vac Membrane 8 – 4 2 3 1 4 4 4 7 1 2 2 9 10 16 11 10 9 0 5 10 49 96
Unique Cell Count 367 258 95 216 258 436 314 430 511 341 354 328 423 389 386 289 265 375 45 266 191 370 397
Labelled Cell Count 371 263 108 228 281 507 363 508 647 438 441 334 430 404 394 298 270 375 45 266 191 370 397


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 – 15.9 12.9 11.2 9.1 8.3 7.9 7.5 6.9 6.4 6.6 17.9 17.4 15.3 21.4 20.2 19.8 18.0 18.1 18.8
Std Deviation (1e-4) 3.0 – 3.2 2.8 2.9 2.5 2.2 1.9 1.7 2.1 1.6 1.7 4.1 5.1 4.8 7.0 6.1 5.7 4.6 4.5 4.3
Intensity Change (Log2) – – – -0.3 -0.5 -0.8 -0.93 -1.01 -1.08 -1.19 -1.31 -1.27 0.17 0.13 -0.05 0.44 0.35 0.32 0.18 0.19 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.5 0.8 0.8 0.9 0 0 0 0 1.9 4.5 5.4 2.7 2.8 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.5 3.7 4.3 6.7 6.7 5.7 0 0 0 0 0 0
Nucleus -1.7 -2.6 -3.9 -4.6 -4.9 -5.4 -6.1 -5.1 -5.3 -1.3 -4.3 -5.8 -3.2 -3.7 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.0 1.5 4.4 5.9 6.5 7.4 7.3 7.5 7.7 -1.6 -1.5 0.5 -1.6 0.7 0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0.9 1.9 1.6 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8978 10.0871 9.3349 8.4432 7.4428 9.2396 8.9077 10.48 9.3163 9.2061 7.4379 9.4494 10.725 10.9537 9.8207 9.7033 7.7769 9.1018
Actin 0.0051 0.0001 0.0009 0 0.0001 0 0.0241 0 0.0104 0 0.0299 0.0003 0.025 0.0106 0.016 0 0.0038 0.0051
Bud 0.0005 0 0.0001 0.0001 0.0001 0 0.0008 0 0.0029 0 0.0021 0.0003 0.0003 0.0008 0.0001 0 0.0001 0.0002
Bud Neck 0.001 0 0.0001 0 0.0009 0.0003 0.001 0 0.0003 0.0001 0.0005 0.0007 0.0007 0.0002 0.0002 0 0.0012 0.0005
Bud Periphery 0.0005 0 0.0001 0 0.0003 0 0.0016 0 0.0054 0 0.0053 0.0005 0.0003 0.001 0.0002 0 0.0001 0.0004
Bud Site 0.0011 0 0.0002 0 0.0008 0 0.0022 0 0.0066 0 0.0039 0.0008 0.0011 0.0051 0.0006 0 0.0018 0.0002
Cell Periphery 0.0001 0 0.0001 0 0.0003 0 0.0006 0 0.0003 0 0.0003 0.0002 0.0002 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0081 0.0263 0.0008 0.0003 0.0012 0 0.0022 0 0.0022 0.0001 0.0003 0.0005 0.0083 0.0001 0.0007 0 0.0033 0.0046
Cytoplasmic Foci 0.0049 0 0.0011 0 0.0164 0 0.0056 0 0.0081 0 0.0026 0.0002 0.0107 0.0004 0.0056 0 0.0168 0.0011
Eisosomes 0.0002 0 0 0 0 0 0.0006 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0044 0.0001 0.002 0 0.0012 0 0.0023 0 0.0014 0 0.0009 0.0002 0.0012 0 0.0015 0 0.0004 0.0006
Endosome 0.0044 0 0.0034 0 0.0129 0 0.0092 0 0.0062 0 0.0121 0.0003 0.0059 0.0005 0.0079 0 0.0074 0.0055
Golgi 0.0012 0 0.0006 0 0.0008 0 0.0039 0 0.0022 0 0.0092 0.0001 0.0027 0.0008 0.0042 0 0.0039 0.0028
Lipid Particles 0.001 0 0.0031 0 0.0145 0 0.0097 0 0.0075 0 0.0037 0.0001 0.0033 0.0002 0.0079 0 0.0072 0.0101
Mitochondria 0.0021 0.0002 0.001 0.0001 0.0008 0.0001 0.0091 0.0003 0.0058 0.0001 0.0952 0.0031 0.0018 0.001 0.0023 0.0001 0.0005 0.0187
None 0.007 0 0.0005 0 0.0008 0 0.0031 0 0.0023 0 0.0005 0.0001 0.0021 0 0.0004 0 0.0007 0.0005
Nuclear Periphery 0.0045 0.0058 0.004 0.0009 0.0069 0.0003 0.0052 0.0005 0.0058 0.0013 0.0017 0.0007 0.0068 0.0011 0.005 0.0022 0.0062 0.0112
Nucleolus 0.0049 0.0079 0.005 0.0033 0.064 0.0156 0.0113 0.0054 0.0082 0.0016 0.0186 0.0157 0.0133 0.0037 0.0028 0.0036 0.0161 0.0217
Nucleus 0.9393 0.9481 0.9744 0.9942 0.8549 0.9834 0.8828 0.9936 0.9154 0.9965 0.789 0.9749 0.8963 0.9693 0.9333 0.9938 0.8895 0.9068
Peroxisomes 0.0006 0 0.0007 0 0.0006 0 0.0134 0 0.0036 0 0.0102 0.0001 0.0034 0.0002 0.0075 0 0.0055 0.0018
Punctate Nuclear 0.0079 0.0111 0.0008 0.001 0.0056 0.0001 0.0038 0.0001 0.0025 0.0001 0.0047 0.0003 0.0149 0.0045 0.0023 0.0001 0.035 0.0061
Vacuole 0.001 0.0002 0.0007 0 0.0141 0.0001 0.0035 0 0.0019 0 0.0036 0.0006 0.0009 0.0001 0.0007 0 0.0003 0.0006
Vacuole Periphery 0.0004 0.0002 0.0004 0 0.0028 0 0.004 0.0001 0.0011 0 0.0053 0.0002 0.0006 0.0001 0.0005 0 0.0002 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.3205 46.5481 61.4057 60.0356 58.9391 41.3031 41.6485 33.0261 35.9262 60.2444
Translational Efficiency 1.182 1.1684 1.1475 0.914 1.0532 1.2948 0.9622 1.0948 0.9739 0.9483

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘