Standard name
Human Ortholog
Description Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.07 0.06 0.05 0.07 0 0.05 0 0.05 0.07 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0.2 0 0.1 0.08 0.06 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.75 0.86 0.8 0.71 0.7 0.67 0.67 0.73 0.78 0.82 0.83 0.76 0.75 0.7 0.86 0.76 0.77 0.7 0.61 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.52 0.32 0.43 0.34 0.54 0.54 0.62 0.64 0.4 0.37 0.32 0.28 0.29 0.23 0.12 0.15 0.22 0.23 0.29 0.35
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 6
Bud Neck 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 2 2 9 0 1 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 1 0 10 4 4 9 4 10 19 21 13 10 12 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 0 0 1
Endosome 0 0 0 1 0 0 0 0 0 3 0 0 2 1 0 1 0 1 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 2
Mitochondria 12 0 7 5 7 6 7 1 6 14 9 8 7 7 0 0 0 0 1 2
Nucleus 83 49 28 96 47 51 80 77 153 383 357 143 111 100 21 85 13 167 229 229
Nuclear Periphery 0 0 1 1 0 0 0 0 0 4 2 0 2 0 0 0 0 0 0 0
Nucleolus 57 18 15 46 36 41 75 68 79 172 139 52 43 33 2 16 3 55 109 150
Peroxisomes 0 0 0 0 0 0 0 0 1 2 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 0 0 0 4 4 0 0 2 0 2 0 0 1 6
Vac/Vac Membrane 1 0 1 0 0 0 0 0 1 3 3 7 7 5 0 3 0 10 23 22
Unique Cell Count 110 57 35 136 67 76 120 106 197 466 432 189 148 142 25 113 17 239 379 434
Labelled Cell Count 160 68 52 159 94 106 174 159 251 605 536 226 184 163 25 113 17 239 379 434


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.4 6.7 8.9 7.5 7.9 8.1 8.5 10.7 10.1 10.9 13.4 12.1 15.4 9.6 9.5 9.5
Std Deviation (1e-4) 2.2 2.6 1.9 2.1 2.1 2.1 2.4 2.1 3.3 2.7 3.7 5.4 4.5 4.7 2.6 3.3 3.2
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9634 3.3927 2.7463 1.8107 1.518 2.2544 4.5091 5.6321 4.5786 4.4026 3.8097 5.3607 5.3226 6.4105 5.5026 5.5221 5.2803 5.6966
Actin 0.0099 0.0001 0.0013 0 0.0009 0 0.0241 0 0.0062 0 0.0417 0.0008 0.0001 0 0.0005 0.0005 0.0006 0
Bud 0.0003 0.0002 0.0005 0 0.0001 0.0001 0.0004 0 0.0005 0 0.001 0.0001 0.0013 0.0003 0.0001 0 0.0001 0.0001
Bud Neck 0.0003 0.0002 0.0005 0 0.0004 0.0011 0.0005 0 0.0043 0 0.0004 0.0006 0.0006 0.0004 0.0001 0.0001 0.0003 0.0009
Bud Periphery 0.0004 0.0003 0.0011 0 0.0004 0.0002 0.0004 0 0.0006 0 0.0018 0.0001 0.003 0.0005 0.0001 0 0.0003 0.0002
Bud Site 0.0013 0.0003 0.0008 0 0.0004 0.0002 0.0013 0 0.0008 0 0.0013 0.0002 0.001 0.0009 0.0001 0.0001 0.0003 0.0002
Cell Periphery 0.0001 0 0.0005 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0007 0 0 0 0 0.0001
Cytoplasm 0.0303 0.0004 0.0159 0.0001 0.0004 0.0002 0.0679 0 0.0011 0 0.0032 0.0001 0.003 0.0001 0.0043 0.0059 0.0163 0
Cytoplasmic Foci 0.0209 0.0002 0.0023 0 0.0014 0.0002 0.0125 0 0.0101 0 0.018 0.0019 0.0005 0.0003 0.0098 0.0002 0.0003 0
Eisosomes 0.0002 0 0 0 0.0001 0 0.0002 0 0 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0023 0.0002 0.0007 0 0.0006 0 0.0031 0 0.0009 0 0.0045 0 0.0003 0 0.0003 0.0005 0.0008 0
Endosome 0.0402 0.0022 0.0022 0 0.007 0.0002 0.0148 0 0.0152 0 0.0238 0.0002 0.0012 0.0001 0.0011 0.0004 0.0012 0
Golgi 0.018 0.0001 0.0005 0 0.0014 0 0.0044 0 0.0062 0 0.0105 0.0003 0.0001 0.0001 0.0003 0 0.0001 0
Lipid Particles 0.03 0.0001 0.0004 0 0.0098 0.0001 0.0103 0 0.0039 0 0.0137 0.0008 0.0002 0.0001 0.0009 0 0.0001 0
Mitochondria 0.0227 0.0014 0.0011 0.0001 0.0081 0.0002 0.0045 0.0002 0.008 0.0002 0.0234 0.0002 0.0009 0.001 0.0004 0.0002 0.0019 0.0002
None 0.03 0.0002 0.0019 0 0.0007 0.0001 0.0222 0 0.0004 0 0.0036 0.0001 0.0007 0.0001 0.005 0.0022 0.0091 0
Nuclear Periphery 0.0241 0.0033 0.0063 0.0002 0.0164 0.0008 0.0277 0.0001 0.0051 0.0002 0.067 0.0004 0.0108 0.0002 0.002 0.0057 0.0074 0.0002
Nucleolus 0.0538 0.0769 0.0574 0.0585 0.2874 0.2327 0.0954 0.0733 0.034 0.0354 0.0408 0.235 0.1001 0.0873 0.0583 0.0777 0.2063 0.1815
Nucleus 0.6354 0.9097 0.8808 0.9409 0.6357 0.7532 0.6721 0.9262 0.8956 0.964 0.6739 0.7278 0.8551 0.9065 0.9068 0.8943 0.7462 0.8159
Peroxisomes 0.0157 0 0.0005 0 0.001 0 0.0042 0 0.0022 0 0.0384 0.0003 0.0001 0.0001 0.0009 0 0.0001 0
Punctate Nuclear 0.0613 0.0008 0.0228 0.0001 0.0239 0.0102 0.0317 0.0001 0.0028 0.0001 0.0286 0.0307 0.0183 0.0017 0.0089 0.0119 0.0081 0.0003
Vacuole 0.0015 0.0021 0.0016 0 0.0015 0.0002 0.0011 0 0.0008 0 0.0009 0.0001 0.0015 0.0002 0.0001 0.0001 0.0003 0.0004
Vacuole Periphery 0.0014 0.0014 0.001 0 0.0019 0.0001 0.0012 0 0.0011 0 0.0031 0 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.3175 12.7069 24.7514 31.2878 29.9484 17.7584 29.7109 28.7244 28.0075 32.6847
Translational Efficiency 2.8334 6.0332 2.9431 2.3948 2.5186 3.7549 2.4734 2.3489 2.5772 1.8822

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
183 1571 288 1330 810 2854 1954 792 993 4425 2242 2122

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 964.93 992.10 1520.51 1374.32 1023.88 1127.19 1283.77 1368.48 1013.02 1079.23 1314.18 1372.14
Standard Deviation 159.22 320.70 266.77 285.34 167.02 195.73 242.80 264.21 167.18 255.74 258.44 277.66
Intensity Change Log 2 0.040061 0.656059 0.510222 0.138684 0.326340 0.418528 0.091676 0.495725 0.463744

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001007 0.000782 0.001627 0.000908 0.001042 0.000531 0.000856 0.001101 0.001036 0.000620 0.000955 0.000980
Bud Neck 0.015270 0.015204 0.024330 0.018883 0.021403 0.019874 0.025707 0.022513 0.020272 0.018216 0.025530 0.020238
Bud Site 0.001146 0.001752 0.003396 0.002314 0.001206 0.001158 0.001568 0.002760 0.001195 0.001369 0.001803 0.002480
Cell Periphery 0.000047 0.000266 0.000071 0.000142 0.000037 0.000051 0.000062 0.000093 0.000039 0.000127 0.000063 0.000123
Cytoplasm 0.000347 0.003534 0.000950 0.000480 0.000087 0.000230 0.000175 0.000854 0.000135 0.001403 0.000275 0.000619
Cytoplasmic Foci 0.000040 0.001417 0.000473 0.000997 0.000041 0.000043 0.000166 0.002700 0.000041 0.000531 0.000205 0.001632
Eisosomes 0.000061 0.000065 0.000145 0.000060 0.000063 0.000058 0.000058 0.000070 0.000062 0.000060 0.000069 0.000064
Endoplasmic Reticulum 0.002850 0.002139 0.001175 0.000726 0.003147 0.001498 0.000795 0.001264 0.003092 0.001726 0.000844 0.000927
Endosome 0.000109 0.000302 0.000057 0.000831 0.000121 0.000146 0.000239 0.004230 0.000119 0.000201 0.000215 0.002099
Golgi 0.000121 0.000419 0.000255 0.001837 0.000167 0.000120 0.000400 0.003505 0.000158 0.000226 0.000381 0.002460
Lipid Particles 0.000071 0.000179 0.000059 0.000067 0.000066 0.000029 0.000029 0.000373 0.000067 0.000082 0.000033 0.000181
Mitochondria 0.001208 0.002096 0.003719 0.002872 0.001496 0.001063 0.002628 0.006198 0.001443 0.001430 0.002768 0.004113
Mitotic Spindle 0.000411 0.002958 0.002858 0.004281 0.001798 0.003504 0.002439 0.017199 0.001542 0.003310 0.002493 0.009102
None 0.001160 0.033129 0.001407 0.001831 0.000745 0.001081 0.001599 0.003888 0.000822 0.012459 0.001574 0.002599
Nuclear Periphery 0.000100 0.000260 0.000204 0.000862 0.000212 0.000203 0.000322 0.000974 0.000191 0.000223 0.000307 0.000904
Nuclear Periphery Foci 0.000071 0.002022 0.000040 0.000724 0.000104 0.000083 0.000077 0.000888 0.000098 0.000772 0.000072 0.000785
Nucleolus 0.088338 0.121950 0.064326 0.048090 0.105435 0.108771 0.088427 0.059754 0.102284 0.113450 0.085331 0.052443
Nucleus 0.885493* 0.809036* 0.884579* 0.908701* 0.860404* 0.860601* 0.871627* 0.861015* 0.865028* 0.842294* 0.873291* 0.890903*
Peroxisomes 0.001602 0.001517 0.008929 0.001483 0.001680 0.000576 0.001588 0.002368 0.001665 0.000910 0.002531 0.001813
Vacuole 0.000340 0.000629 0.001109 0.002164 0.000413 0.000157 0.000620 0.004819 0.000399 0.000324 0.000682 0.003155
Vacuole Periphery 0.000207 0.000345 0.000294 0.001750 0.000335 0.000225 0.000621 0.003433 0.000312 0.000267 0.000579 0.002378

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.73 -9.11 0.25 -3.57 10.23 11.49* 3.17 -2.58 -9.07 -4.58 10.57 -0.28 -0.68 -11.09 -0.51
Bud Neck 0.25 -6.60 -2.87 -5.53 5.38 1.64 -4.26 -2.14 -3.85 1.51 2.40 -6.06 -1.90 -5.41 4.68
Bud Site -1.75 -7.47 -2.08 -1.00 4.99 1.11 -0.43 -3.47 -5.67 -4.17 0.41 -2.67 -2.70 -3.71 -0.98
Cell Periphery -6.56 -4.67 -2.35 3.03 -0.54 -1.57 -1.66 -4.81 -3.58 -2.16 -6.97 -2.31 -3.60 0.40 -2.14
Cytoplasm -2.89 0.35 0.58 5.51 0.49 -0.89 -0.16 -2.90 -2.59 -3.02 -4.48 0.39 -1.95 3.28 -3.55
Cytoplasmic Foci -3.68 -6.52 -2.37 1.47 0.21 0.15 -0.45 -2.58 -2.61 -2.51 -3.23 -1.74 -3.37 -1.58 -2.89
Eisosomes -0.66 -11.89 -0.68 0.11 12.03 2.92 1.51 -3.79 -6.90 -5.67 1.72 -4.36 -2.05 -3.95 2.52
Endoplasmic Reticulum 2.43 4.70 8.06 10.29 6.44 12.10 17.95 7.96 0.15 -3.78 10.91 18.63 15.70 7.76 -1.67
Endosome -2.44 -0.07 -2.26 -1.26 -2.26 -0.60 -0.70 -2.97 -2.95 -2.87 -2.33 -0.71 -3.61 -3.36 -3.35
Golgi -1.91 -8.99 -1.92 -1.39 -1.29 1.94 -0.70 -2.51 -2.62 -2.28 -0.48 -1.03 -2.97 -2.90 -2.49
Lipid Particles -2.46 -1.78 0.20 2.58 1.97 2.55 3.69 -1.85 -2.22 -2.25 -0.75 2.81 -1.62 -1.22 -2.34
Mitochondria -1.38 -15.68 -10.71 -1.76 7.01 5.20 -9.03 -4.47 -4.98 -2.90 0.30 -12.36 -7.21 -6.39 -2.25
Mitotic Spindle -2.77 -3.67 -2.89 -0.62 2.70 -1.01 -0.44 -3.84 -3.48 -3.75 -1.43 -2.11 -3.81 -3.06 -2.25
None -8.48 -2.53 -2.07 8.29 -0.00 -3.39 -1.56 -2.76 -2.40 -1.73 -8.58 -1.70 -4.14 7.06 -1.49
Nuclear Periphery -2.88 -5.43 -2.78 -2.13 -1.62 0.51 -1.21 -2.79 -2.86 -2.23 -0.98 -1.76 -3.56 -3.41 -2.60
Nuclear Periphery Foci -3.97 -0.40 -2.04 2.70 -1.95 1.05 1.51 -2.79 -2.99 -3.08 -3.79 1.38 -2.88 0.84 -3.12
Nucleolus -3.43 -1.21 3.86 14.44 5.98 -0.66 1.64 5.19 6.73 4.24 -2.47 0.99 9.15 15.86 9.52
Nucleus 6.85 5.05 -1.79 -13.29 -7.60 -0.36 -0.14 0.85 1.18 1.00 4.08 1.84 -3.48 -8.42 -5.64
Peroxisomes 0.23 -7.04 -0.63 -0.97 6.97 8.09 -0.10 -2.90 -6.74 -2.88 6.38 -5.38 -1.93 -7.77 3.89
Vacuole -1.23 -5.78 -2.38 -1.96 0.09 4.05 -0.73 -4.06 -4.50 -3.91 1.75 -2.09 -4.40 -4.76 -3.74
Vacuole Periphery -2.61 -6.02 -2.07 -1.86 -1.66 2.00 -1.71 -3.05 -3.24 -2.63 1.11 -2.00 -3.33 -3.48 -2.65