Standard name
Human Ortholog
Description Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.12 0.09 0.11 0.12 0.08 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.99 0.98 0.96 0.87 0.86 0.77 0.76 0.75 0.73 0.99 0.99 0.98 0.97 0.96 0.97 0.97 0.97 0.9 0.97 0.96 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0.09 0.09 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.09 0.06 0.12 0.17 0.15 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 5 5 1 5 2 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 1 6 1 3 0 2 2 2 1 0 1 3 2 0 0 0 0 0 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 12 2 0 2 5 6 8 21 16 11 18 38 15 23 11 7 6 0 0 0 0 0 0
Cytoplasm 268 376 157 102 152 143 159 184 135 139 75 109 459 620 575 340 232 121 258 364 150 261 365 374
Endoplasmic Reticulum 0 3 0 0 2 1 4 16 15 16 8 8 2 6 4 9 6 5 3 0 5 1 4 20
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 2
Mitochondria 0 2 1 2 4 3 16 13 22 32 15 34 0 0 1 5 1 0 0 1 1 0 0 1
Nucleus 0 0 0 0 0 1 1 1 2 0 0 0 0 0 1 0 2 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 2 0 8 2 1 8 1 4 3 1 2 6 8 2 0 2 4 2 4 5
Unique Cell Count 269 379 159 103 155 149 183 214 176 184 100 150 463 627 586 349 242 125 265 376 167 269 382 421
Labelled Cell Count 271 395 167 105 165 153 198 231 203 215 116 177 505 645 606 371 258 136 265 376 167 269 382 421


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.3 17.1 12.6 12.4 13.0 13.1 10.5 10.9 10.6 10.2 9.7 9.9 18.9 18.8 19.1 16.5 20.6 22.1 16.2 17.2 18.8
Std Deviation (1e-4) 2.6 3.5 2.3 2.1 2.8 2.6 2.6 2.5 2.0 2.6 2.0 2.4 3.4 3.8 4.0 5.8 7.4 7.9 3.0 3.5 4.2
Intensity Change (Log2) – – – -0.02 0.05 0.05 -0.27 -0.21 -0.25 -0.3 -0.37 -0.35 0.59 0.58 0.6 0.39 0.71 0.81 0.37 0.45 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.9 3.1 3.5 3.8 3.1 0 1.7 0 0 0
Cytoplasm 0.2 -0.5 -1.5 -4.1 -4.4 -6.0 -6.2 -6.1 -6.6 0.4 0.1 -0.5 -0.9 -1.7 -1.1
Endoplasmic Reticulum 0 0 0 0 3.5 3.8 3.8 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.4 2.7 4.3 5.2 4.7 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1451 16.1227 16.7693 13.1131 16.2824 16.9247 10.9229 14.1966 13.2479 13.3071 10.4332 12.8542 12.6262 17.9842 15.6777 16.3371 14.0914 16.3118
Actin 0.0153 0.046 0.0005 0.0224 0.0002 0.0008 0.0261 0.0062 0.017 0.0002 0.0107 0.0014 0.0378 0.0012 0.0172 0.0079 0.0569 0.0077
Bud 0.0002 0.0008 0.0001 0.0006 0.0002 0.0005 0.0003 0.0008 0.0001 0.0001 0.0003 0.0003 0.0005 0.0002 0.0005 0.0216 0.0003 0.0001
Bud Neck 0.0005 0.0005 0.0004 0.0006 0.0006 0.0015 0.0005 0.0002 0.0002 0.0002 0.0004 0.0008 0.0019 0.0002 0.0014 0.0006 0.0006 0.0014
Bud Periphery 0.0001 0.0008 0 0.0016 0 0.0003 0.0002 0.0002 0 0 0.0001 0.0003 0.0006 0 0.0004 0.0062 0.0002 0.0001
Bud Site 0.0008 0.0023 0.0001 0.0015 0 0.0001 0.004 0.002 0.0002 0 0.0005 0.001 0.0013 0.0002 0.0012 0.0018 0.0005 0.0002
Cell Periphery 0.0002 0.0003 0.0001 0.0004 0 0 0.0001 0.0001 0 0 0.0001 0 0.0003 0 0.0002 0.0002 0.0001 0
Cytoplasm 0.8136 0.8536 0.9825 0.842 0.9891 0.9801 0.8774 0.9783 0.9537 0.993 0.912 0.9673 0.8342 0.9915 0.9419 0.8937 0.7469 0.9757
Cytoplasmic Foci 0.0236 0.0038 0.0008 0.0094 0.0027 0.0017 0.0132 0.007 0.003 0.0021 0.0172 0.0066 0.0217 0.0009 0.0047 0.014 0.0466 0.0032
Eisosomes 0.0002 0.0004 0 0.0003 0 0 0.0005 0 0 0 0.0001 0 0.0006 0 0.0002 0.0001 0.0007 0
Endoplasmic Reticulum 0.0171 0.0029 0.0097 0.0084 0.0011 0.0036 0.0095 0.0014 0.0008 0.0006 0.0032 0.0034 0.0207 0.0032 0.0024 0.0012 0.0031 0.0026
Endosome 0.028 0.0136 0.0012 0.0125 0.002 0.0023 0.0082 0.0005 0.0013 0.0021 0.0199 0.0073 0.0307 0.0007 0.008 0.0052 0.0271 0.003
Golgi 0.0046 0.0278 0.0001 0.0054 0.0001 0.0008 0.0051 0.0003 0.0013 0.0002 0.0101 0.0026 0.0091 0.0001 0.0029 0.0075 0.0148 0.0017
Lipid Particles 0.0137 0.0015 0 0.0146 0 0 0.0131 0.0001 0.0126 0 0.013 0.0001 0.0074 0 0.0041 0.0096 0.0389 0.0014
Mitochondria 0.001 0.0378 0.0004 0.0444 0.0001 0.0052 0.0022 0.0002 0.0006 0 0.0053 0.006 0.0084 0 0.0045 0.0107 0.0028 0.0004
None 0.0179 0.0006 0.0003 0.0026 0.0002 0.0003 0.0118 0.0006 0.0006 0.0002 0.0012 0.0004 0.0058 0.0003 0.003 0.0024 0.0154 0.0003
Nuclear Periphery 0.0084 0.0014 0.0013 0.0134 0.0006 0.0005 0.0126 0.0003 0.0002 0.0002 0.0006 0.0004 0.0067 0.0006 0.0008 0.0004 0.0051 0.0004
Nucleolus 0.0011 0 0 0.0003 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0001 0.0003 0.0003 0
Nucleus 0.0033 0.0013 0.0015 0.0017 0.0013 0.0014 0.0015 0.0006 0.0005 0.0006 0.0008 0.0008 0.0028 0.0006 0.0009 0.0013 0.0009 0.0008
Peroxisomes 0.0075 0.0013 0 0.0021 0 0 0.0095 0.0004 0.0073 0 0.0029 0.0002 0.0032 0 0.0013 0.0134 0.0269 0.0005
Punctate Nuclear 0.0397 0.0002 0 0.0056 0.0001 0 0.0019 0.0005 0.0001 0 0.0006 0.0001 0.0019 0 0.0005 0.0004 0.0106 0.0001
Vacuole 0.0026 0.0023 0.0007 0.0049 0.0013 0.0005 0.0014 0.0003 0.0003 0.0003 0.0007 0.0006 0.003 0.0002 0.0028 0.0011 0.0008 0.0002
Vacuole Periphery 0.0006 0.0008 0.0003 0.0053 0.0003 0.0003 0.0007 0 0.0001 0.0001 0.0004 0.0002 0.0012 0 0.0011 0.0006 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 57.673 52.5289 51.0092 68.1404 44.6772 74.6796 61.4721 68.1535 70.1116 65.5662
Translational Efficiency 3.3406 2.523 2.8654 2.4043 3.072 2.7292 2.1495 2.2184 2.3882 2.3327

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1997 898 601 1399 1062 1607 181 528 3059 2505 782 1927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1432.68 1410.90 2084.39 1880.43 1377.30 1514.98 2052.29 1924.81 1413.45 1477.67 2076.96 1892.59
Standard Deviation 207.90 327.74 289.04 327.76 209.96 243.63 250.35 365.41 210.28 281.20 280.88 339.07
Intensity Change Log 2 — -0.022101 0.540909 0.392346 — 0.137456 0.575392 0.482873 — 0.058311 0.557913 0.437428

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000065 0.000229 0.000377 0.000520 0.000258 0.000176 0.000667 0.000599 0.000132 0.000195 0.000444 0.000542
Bud Neck 0.000501 0.000413 0.004186 0.001786 0.000387 0.000251 0.004589 0.002497 0.000461 0.000309 0.004279 0.001981
Bud Site 0.007598 0.012368 0.010215 0.016446 0.007969 0.005910 0.011981 0.017762 0.007727 0.008225 0.010623 0.016806
Cell Periphery 0.000166 0.000189 0.000969 0.000736 0.000192 0.000099 0.000954 0.001992 0.000175 0.000131 0.000966 0.001080
Cytoplasm 0.933038* 0.923721* 0.748234* 0.831556* 0.888112* 0.922243* 0.698425* 0.804383* 0.917441* 0.922772* 0.736705* 0.824111*
Cytoplasmic Foci 0.022421 0.025411 0.025624 0.012897 0.056497 0.033463 0.035213 0.020497 0.034251 0.030577 0.027844 0.014980
Eisosomes 0.000005 0.000003 0.000004 0.000004 0.000003 0.000002 0.000007 0.000006 0.000004 0.000002 0.000004 0.000004
Endoplasmic Reticulum 0.000206 0.000787 0.000628 0.000293 0.003164 0.001251 0.000636 0.000320 0.001233 0.001085 0.000630 0.000300
Endosome 0.000340 0.001952 0.005632 0.004040 0.003790 0.002644 0.010189 0.006415 0.001538 0.002396 0.006687 0.004691
Golgi 0.004196 0.004134 0.005913 0.005057 0.006028 0.005448 0.004131 0.009198 0.004832 0.004977 0.005501 0.006192
Lipid Particles 0.000370 0.001405 0.008631 0.006959 0.001208 0.000769 0.011065 0.011405 0.000661 0.000997 0.009194 0.008177
Mitochondria 0.000524 0.000110 0.000050 0.001219 0.000198 0.000199 0.000022 0.000223 0.000411 0.000167 0.000044 0.000946
Mitotic Spindle 0.000103 0.000726 0.005265 0.004793 0.001454 0.002434 0.003538 0.001617 0.000572 0.001822 0.004865 0.003923
None 0.008482 0.003145 0.000908 0.000430 0.001334 0.000922 0.000459 0.000386 0.006000 0.001719 0.000804 0.000418
Nuclear Periphery 0.000008 0.000070 0.000314 0.000074 0.000034 0.000060 0.000283 0.000054 0.000017 0.000063 0.000307 0.000069
Nuclear Periphery Foci 0.000211 0.002869 0.008417 0.002197 0.002502 0.002017 0.013494 0.003435 0.001006 0.002323 0.009592 0.002536
Nucleolus 0.000160 0.000098 0.000118 0.000182 0.000046 0.000152 0.000066 0.000283 0.000120 0.000133 0.000106 0.000210
Nucleus 0.010977 0.008611 0.024412 0.007699 0.005905 0.005849 0.018799 0.007237 0.009216 0.006839 0.023113 0.007573
Peroxisomes 0.000246 0.000312 0.000121 0.000571 0.000140 0.000377 0.000335 0.000460 0.000210 0.000354 0.000170 0.000541
Vacuole 0.008576 0.012367 0.146196 0.095222 0.017294 0.013420 0.183890 0.108486 0.011603 0.013043 0.154921 0.098856
Vacuole Periphery 0.001808 0.001080 0.003786 0.007319 0.003487 0.002313 0.001258 0.002745 0.002391 0.001871 0.003201 0.006066

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.79 -9.10 -4.40 -2.65 -0.14 2.53 -5.80 -2.49 -2.96 -0.05 -3.55 -9.98 -4.87 -4.10 -0.23
Bud Neck 1.39 -11.48 -12.19 -12.54 7.17 3.43 -10.59 -9.07 -9.56 3.75 4.52 -14.07 -15.25 -16.41 7.89
Bud Site -2.68 -4.24 -6.31 -1.55 -2.35 2.47 -3.48 -6.99 -8.42 -2.88 -0.63 -5.23 -8.67 -6.96 -3.41
Cell Periphery -1.42 -8.26 -9.41 -8.73 2.91 5.39 -4.70 -8.17 -8.66 -4.20 4.54 -9.36 -11.94 -12.57 -0.18
Cytoplasm 2.15 19.74 19.99 15.17 -8.02 -5.35 11.06 9.41 12.76 -5.32 -1.27 21.91 20.74 20.23 -9.44
Cytoplasmic Foci -1.58 -3.44 7.63 6.09 7.63 6.64 4.45 9.76 4.42 4.21 1.95 1.29 12.82 9.69 8.44
Eisosomes 5.06 -0.51 1.85 -2.87 2.05 2.27 -6.44 -3.75 -4.63 1.35 6.04 -3.77 -1.05 -5.52 2.21
Endoplasmic Reticulum -1.68 -2.94 -0.54 1.58 2.68 3.04 4.59 5.18 3.12 2.65 0.50 2.88 4.94 3.58 3.23
Endosome -4.09 -11.74 -6.73 -2.39 4.28 1.99 -4.28 -1.38 -2.27 2.15 -2.60 -10.88 -5.03 -2.84 4.48
Golgi 0.13 -3.63 0.35 0.12 2.40 0.69 4.41 -0.63 -0.87 -1.84 -0.22 -0.92 -0.11 0.05 0.32
Lipid Particles -2.93 -12.22 -10.07 -7.88 2.37 2.78 -7.29 -7.84 -8.14 -1.19 -2.27 -13.98 -12.63 -11.88 1.38
Mitochondria 4.33 5.64 -0.92 -2.05 -2.20 0.00 1.40 0.10 0.12 -1.15 2.85 5.23 -0.97 -1.87 -2.34
Mitotic Spindle -5.39 -7.59 -3.86 -3.27 1.75 -0.91 -1.78 -0.12 0.89 1.79 -2.40 -7.13 -3.51 -1.90 2.35
None 4.72 13.66 14.24 2.69 2.54 1.55 3.48 3.71 4.44 0.59 8.22 13.74 14.46 3.48 2.70
Nuclear Periphery -1.45 -9.12 -7.41 -0.38 7.10 -0.92 -4.92 -3.37 -0.35 4.38 -2.00 -10.27 -7.82 -0.74 8.33
Nuclear Periphery Foci -6.73 -10.84 -9.51 0.76 7.72 0.85 -5.19 -3.18 -3.06 4.46 -3.57 -11.44 -8.52 -1.42 8.82
Nucleolus 2.18 2.26 -1.68 -2.81 -3.07 -0.98 -2.51 -1.66 -0.48 -1.43 -0.34 -0.16 -2.28 -0.61 -2.23
Nucleus 1.79 -9.18 0.11 -1.55 8.86 0.25 -6.39 -2.78 -3.11 4.96 4.07 -11.28 -1.84 -4.74 10.14
Peroxisomes -0.58 4.81 -3.50 -1.44 -4.74 -0.96 -3.46 -4.96 -0.23 -0.99 -0.89 0.46 -4.95 -0.75 -4.93
Vacuole -2.80 -16.78 -25.09 -22.90 5.17 1.78 -11.27 -13.62 -14.14 4.55 -1.64 -19.94 -28.00 -27.04 6.64
Vacuole Periphery 2.02 -3.07 -3.72 -4.58 -2.11 1.50 3.77 1.39 0.06 -1.93 1.24 -0.94 -2.89 -3.55 -2.35
Standard name
Human Ortholog
Description Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Cdc37

Cdc37


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc37-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available