Standard name
Description Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.12 0.09 0.11 0.12 0.08 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.99 0.98 0.96 0.87 0.86 0.77 0.76 0.75 0.73 0.99 0.99 0.98 0.97 0.96 0.97 0.97 0.97 0.9 0.97 0.96 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0.09 0.09 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.09 0.06 0.12 0.17 0.15 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 5 5 1 5 2 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 1 6 1 3 0 2 2 2 1 0 1 3 2 0 0 0 0 0 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 12 2 0 2 5 6 8 21 16 11 18 38 15 23 11 7 6 0 0 0 0 0 0
Cytoplasm 268 376 157 102 152 143 159 184 135 139 75 109 459 620 575 340 232 121 258 364 150 261 365 374
Endoplasmic Reticulum 0 3 0 0 2 1 4 16 15 16 8 8 2 6 4 9 6 5 3 0 5 1 4 20
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 2
Mitochondria 0 2 1 2 4 3 16 13 22 32 15 34 0 0 1 5 1 0 0 1 1 0 0 1
Nucleus 0 0 0 0 0 1 1 1 2 0 0 0 0 0 1 0 2 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 3
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 2 0 8 2 1 8 1 4 3 1 2 6 8 2 0 2 4 2 4 5
Unique Cell Count 269 379 159 103 155 149 183 214 176 184 100 150 463 627 586 349 242 125 265 376 167 269 382 421
Labelled Cell Count 271 395 167 105 165 153 198 231 203 215 116 177 505 645 606 371 258 136 265 376 167 269 382 421


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.3 17.1 12.6 12.4 13.0 13.1 10.5 10.9 10.6 10.2 9.7 9.9 18.9 18.8 19.1 16.5 20.6 22.1 16.2 17.2 18.8
Std Deviation (1e-4) 2.6 3.5 2.3 2.1 2.8 2.6 2.6 2.5 2.0 2.6 2.0 2.4 3.4 3.8 4.0 5.8 7.4 7.9 3.0 3.5 4.2
Intensity Change (Log2) -0.02 0.05 0.05 -0.27 -0.21 -0.25 -0.3 -0.37 -0.35 0.59 0.58 0.6 0.39 0.71 0.81 0.37 0.45 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.9 3.1 3.5 3.8 3.1 0 1.7 0 0 0
Cytoplasm 0.2 -0.5 -1.5 -4.1 -4.4 -6.0 -6.2 -6.1 -6.6 0.4 0.1 -0.5 -0.9 -1.7 -1.1
Endoplasmic Reticulum 0 0 0 0 3.5 3.8 3.8 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.4 2.7 4.3 5.2 4.7 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc37-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available