Standard name | |
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Human Ortholog | |
Description | Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 4 | 0 | 1 | 0 | 3 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Bud | 0 | 16 | 14 | 9 | 17 | 17 | 29 | 25 | 34 | 29 | 13 | 24 | 0 | 2 | 0 | 1 | – | 0 | 3 | 1 | 1 | 2 | 5 | 6 |
Bud Neck | 1 | 9 | 19 | 15 | 11 | 18 | 21 | 31 | 20 | 18 | 6 | 17 | 1 | 9 | 1 | 0 | – | 0 | 0 | 2 | 2 | 0 | 0 | 2 |
Bud Site | 1 | 4 | 5 | 4 | 11 | 9 | 18 | 2 | 16 | 15 | 10 | 15 | 0 | 0 | 0 | 0 | – | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 22 | 14 | 20 | 23 | 17 | 16 | 18 | 34 | 21 | 20 | 14 | 2 | 23 | 44 | 1 | – | 3 | 6 | 13 | 7 | 11 | 4 | 15 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 14 | 59 | 16 | 7 | 0 | 8 | 3 | 5 | 3 | 4 | 7 | 2 | 2 | 16 | 43 | 0 | – | 3 | 10 | 41 | 22 | 16 | 9 | 9 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 21 | 61 | 4 | 7 | 15 | 17 | 30 | 18 | 62 | 31 | 51 | 64 | 0 | 1 | 0 | 3 | – | 1 | 0 | 11 | 0 | 1 | 0 | 4 |
Nucleus | 1 | 3 | 4 | 2 | 3 | 5 | 6 | 6 | 8 | 7 | 12 | 8 | 0 | 5 | 3 | 0 | – | 0 | 0 | 0 | 0 | 11 | 7 | 8 |
Nuclear Periphery | 2 | 0 | 0 | 2 | 2 | 1 | 5 | 6 | 9 | 6 | 3 | 8 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 1 | 2 | 0 |
Nucleolus | 32 | 24 | 29 | 36 | 41 | 38 | 40 | 39 | 72 | 59 | 51 | 59 | 9 | 45 | 32 | 7 | – | 8 | 0 | 7 | 4 | 11 | 9 | 15 |
Peroxisomes | 8 | 3 | 0 | 6 | 0 | 8 | 1 | 1 | 15 | 3 | 6 | 5 | 0 | 1 | 12 | 0 | – | 0 | 0 | 3 | 1 | 2 | 0 | 1 |
SpindlePole | 70 | 222 | 170 | 133 | 118 | 158 | 194 | 197 | 176 | 188 | 93 | 144 | 15 | 106 | 88 | 12 | – | 11 | 98 | 295 | 215 | 135 | 139 | 160 |
Vac/Vac Membrane | 2 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | – | 0 | 0 | 1 | 0 | 3 | 1 | 4 |
Unique Cell Count | 111 | 315 | 213 | 183 | 177 | 223 | 253 | 257 | 305 | 274 | 196 | 250 | 17 | 155 | 155 | 17 | 16 | 125 | 387 | 267 | 205 | 184 | 236 | |
Labelled Cell Count | 165 | 427 | 282 | 242 | 243 | 298 | 366 | 351 | 455 | 385 | 273 | 361 | 29 | 210 | 224 | 26 | 29 | 125 | 387 | 267 | 205 | 184 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 3.8 | 5.8 | 5.5 | 4.5 | 5.2 | 4.8 | 5.5 | 4.5 | 4.9 | 5.3 | 4.4 | 4.8 | 5.5 | 5.4 | 6.0 | – | 6.9 | 6.6 | 7.1 | 6.9 |
Std Deviation (1e-4) | 1.1 | 0.5 | 1.7 | 1.8 | 1.4 | 2.0 | 1.7 | 2.2 | 2.1 | 2.0 | 6.0 | 1.7 | 0.7 | 1.1 | 1.7 | 1.1 | – | 2.4 | 2.5 | 3.0 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.37 | -0.16 | -0.26 | -0.08 | -0.35 | -0.24 | -0.11 | -0.39 | -0.28 | -0.07 | -0.1 | 0.06 | – | 0.25 | 0.18 | 0.3 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4453 | 4.076 | 3.8103 | 3.7322 | 3.8368 | 3.528 | 5.0434 | 4.7839 | 5.7672 | 4.8642 | 3.744 | 5.169 | 2.9242 | 2.9923 | 3.1171 | 3.3592 | 3.6539 | 3.0519 |
Actin | 0.0234 | 0.0013 | 0.0017 | 0.0006 | 0.0051 | 0.0006 | 0.0301 | 0.0006 | 0.0036 | 0.0256 | 0.0002 | 0.0096 | 0.0091 | 0.0004 | 0.0039 | 0.0253 | 0.0165 | 0.001 |
Bud | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0003 | 0 | 0.0009 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0001 | 0.0001 |
Bud Neck | 0.0004 | 0.0013 | 0.0074 | 0.0005 | 0.0008 | 0.0005 | 0.0023 | 0.0002 | 0.0006 | 0.0007 | 0.0003 | 0.0002 | 0.0007 | 0.0026 | 0.0023 | 0.0021 | 0.0007 | 0.0013 |
Bud Periphery | 0.0001 | 0.0005 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0014 | 0.0001 | 0.0004 |
Bud Site | 0.0003 | 0.0118 | 0.0052 | 0.0001 | 0.0016 | 0.0003 | 0.0016 | 0.0002 | 0.0011 | 0.0015 | 0.0003 | 0.0002 | 0.0021 | 0.0088 | 0.0019 | 0.0186 | 0.001 | 0.0005 |
Cell Periphery | 0 | 0.001 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 |
Cytoplasm | 0.0014 | 0.0258 | 0.0013 | 0.0002 | 0.0116 | 0.0013 | 0.0063 | 0.0648 | 0.0022 | 0.0003 | 0.0002 | 0.0004 | 0.0022 | 0.0084 | 0.0032 | 0.0005 | 0.0032 | 0.0013 |
Cytoplasmic Foci | 0.1619 | 0.1547 | 0.1425 | 0.1172 | 0.2362 | 0.1061 | 0.1169 | 0.1673 | 0.139 | 0.1302 | 0.1851 | 0.1035 | 0.0675 | 0.0572 | 0.0306 | 0.0925 | 0.1542 | 0.0491 |
Eisosomes | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0006 | 0.0008 | 0.0001 | 0 | 0.0024 | 0.0001 | 0.0018 | 0.0011 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0006 | 0.0005 | 0.0002 |
Endosome | 0.017 | 0.0164 | 0.0117 | 0.0012 | 0.0873 | 0.0064 | 0.016 | 0.0029 | 0.0212 | 0.0082 | 0.0021 | 0.0058 | 0.0078 | 0.0036 | 0.0095 | 0.0072 | 0.0305 | 0.0059 |
Golgi | 0.0114 | 0.0018 | 0.0039 | 0.0017 | 0.0157 | 0.009 | 0.0214 | 0.0013 | 0.0167 | 0.0161 | 0.0021 | 0.0108 | 0.0025 | 0.0014 | 0.0067 | 0.0019 | 0.0101 | 0.0017 |
Lipid Particles | 0.0215 | 0.0175 | 0.0078 | 0.0095 | 0.1186 | 0.0297 | 0.0259 | 0.0047 | 0.0513 | 0.0743 | 0.0103 | 0.0061 | 0.0198 | 0.0064 | 0.0161 | 0.0038 | 0.0719 | 0.0107 |
Mitochondria | 0.006 | 0.0078 | 0.0023 | 0 | 0.0091 | 0.0165 | 0.0041 | 0 | 0.0023 | 0.0219 | 0 | 0.0035 | 0.0021 | 0.0008 | 0.0039 | 0.0007 | 0.002 | 0.0006 |
None | 0.0061 | 0.0055 | 0.005 | 0.0002 | 0.0029 | 0.0153 | 0.0682 | 0.0872 | 0.0195 | 0.0006 | 0.0003 | 0.0039 | 0.0115 | 0.0163 | 0.0044 | 0.0005 | 0.003 | 0.0025 |
Nuclear Periphery | 0.0007 | 0.0019 | 0.0004 | 0.0006 | 0.0287 | 0.0019 | 0.0051 | 0.0003 | 0.0027 | 0.0005 | 0.0003 | 0.0006 | 0.0022 | 0.0024 | 0.0044 | 0.0019 | 0.0095 | 0.0035 |
Nucleolus | 0.0009 | 0.0247 | 0.0047 | 0.0004 | 0.0014 | 0.0019 | 0.0039 | 0.0006 | 0.0043 | 0.0008 | 0.0011 | 0.0006 | 0.0035 | 0.0226 | 0.0187 | 0.003 | 0.0017 | 0.0101 |
Nucleus | 0.0182 | 0.0226 | 0.0031 | 0.0002 | 0.0126 | 0.013 | 0.0044 | 0.0004 | 0.0015 | 0.0003 | 0.0004 | 0.0003 | 0.0022 | 0.0534 | 0.0152 | 0.0025 | 0.0174 | 0.0088 |
Peroxisomes | 0.0418 | 0.018 | 0.0133 | 0.0127 | 0.041 | 0.008 | 0.0397 | 0.0212 | 0.0254 | 0.0937 | 0.0186 | 0.0151 | 0.0131 | 0.0118 | 0.0186 | 0.0086 | 0.072 | 0.0097 |
Punctate Nuclear | 0.6879 | 0.6819 | 0.7876 | 0.8548 | 0.4196 | 0.7887 | 0.6466 | 0.647 | 0.7073 | 0.6233 | 0.7787 | 0.8387 | 0.8517 | 0.8017 | 0.8583 | 0.7864 | 0.603 | 0.8908 |
Vacuole | 0.0002 | 0.0039 | 0.0008 | 0 | 0.003 | 0.0002 | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0008 | 0.0005 | 0.0308 | 0.0015 | 0.0013 |
Vacuole Periphery | 0.0002 | 0.0002 | 0.0003 | 0 | 0.002 | 0.0003 | 0.0007 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0106 | 0.0011 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.3414 | 7.9364 | 0.8413 | 5.4123 | 17.266 | 8.8731 | 6.3049 | 5.0291 | 6.26 | 11.3954 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |