Standard name
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0.05 0.07 0.05 0.1 0.08 0.11 0.1 0.11 0.11 0.07 0.1 0 0 0 0.06 – 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0.08 0.06 0.08 0.08 0.12 0.07 0.07 0 0.07 0.06 0.06 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0.06 0 0.07 0 0.05 0.05 0.05 0.06 0 0 0 0 – 0.12 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.12 0.07 0.07 0.11 0.13 0.08 0.06 0.07 0.11 0.08 0.1 0.06 0.12 0.15 0.28 0.06 – 0.19 0.05 0 0 0.05 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – 0.06 0 0 0 0 0 0
Endosome 0.13 0.19 0.08 0 0 0 0 0 0 0 0 0 0.12 0.1 0.28 0 – 0.19 0.08 0.11 0.08 0.08 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.19 0.19 0 0 0.08 0.08 0.12 0.07 0.2 0.11 0.26 0.26 0 0 0 0.18 – 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.29 0.08 0.14 0.2 0.23 0.17 0.16 0.15 0.24 0.22 0.26 0.24 0.53 0.29 0.21 0.41 – 0.5 0 0 0 0.06 0.05 0.06
Peroxisomes 0.07 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.08 0 – 0 0 0 0 0 0 0
SpindlePole 0.63 0.7 0.8 0.73 0.67 0.71 0.77 0.77 0.58 0.69 0.47 0.58 0.88 0.68 0.57 0.71 – 0.69 0.79 0.76 0.81 0.66 0.76 0.68
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 0 1 0 3 2 4 2 0 0 0 0 0 0 – 0 0 1 0 0 0 2
Bud 0 16 14 9 17 17 29 25 34 29 13 24 0 2 0 1 – 0 3 1 1 2 5 6
Bud Neck 1 9 19 15 11 18 21 31 20 18 6 17 1 9 1 0 – 0 0 2 2 0 0 2
Bud Site 1 4 5 4 11 9 18 2 16 15 10 15 0 0 0 0 – 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 13 22 14 20 23 17 16 18 34 21 20 14 2 23 44 1 – 3 6 13 7 11 4 15
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 2 – 1 0 0 0 0 0 1
Endosome 14 59 16 7 0 8 3 5 3 4 7 2 2 16 43 0 – 3 10 41 22 16 9 9
Golgi 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0
Mitochondria 21 61 4 7 15 17 30 18 62 31 51 64 0 1 0 3 – 1 0 11 0 1 0 4
Nucleus 1 3 4 2 3 5 6 6 8 7 12 8 0 5 3 0 – 0 0 0 0 11 7 8
Nuclear Periphery 2 0 0 2 2 1 5 6 9 6 3 8 0 0 0 0 – 0 0 0 3 1 2 0
Nucleolus 32 24 29 36 41 38 40 39 72 59 51 59 9 45 32 7 – 8 0 7 4 11 9 15
Peroxisomes 8 3 0 6 0 8 1 1 15 3 6 5 0 1 12 0 – 0 0 3 1 2 0 1
SpindlePole 70 222 170 133 118 158 194 197 176 188 93 144 15 106 88 12 – 11 98 295 215 135 139 160
Vac/Vac Membrane 2 1 3 0 1 2 0 0 2 1 1 1 0 2 1 0 – 0 0 1 0 3 1 4
Unique Cell Count 111 315 213 183 177 223 253 257 305 274 196 250 17 155 155 17 16 125 387 267 205 184 236
Labelled Cell Count 165 427 282 242 243 298 366 351 455 385 273 361 29 210 224 26 29 125 387 267 205 184 236


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.8 5.8 5.5 4.5 5.2 4.8 5.5 4.5 4.9 5.3 4.4 4.8 5.5 5.4 6.0 – 6.9 6.6 7.1 6.9
Std Deviation (1e-4) 1.1 0.5 1.7 1.8 1.4 2.0 1.7 2.2 2.1 2.0 6.0 1.7 0.7 1.1 1.7 1.1 – 2.4 2.5 3.0 2.7
Intensity Change (Log2) – – – -0.07 -0.37 -0.16 -0.26 -0.08 -0.35 -0.24 -0.11 -0.39 -0.28 -0.07 -0.1 0.06 – 0.25 0.18 0.3 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtw1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available