| Standard name | |
|---|---|
| Description | Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 1 | 4 | 0 | 1 | 0 | 3 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
| Bud | 0 | 16 | 14 | 9 | 17 | 17 | 29 | 25 | 34 | 29 | 13 | 24 | 0 | 2 | 0 | 1 | – | 0 | 3 | 1 | 1 | 2 | 5 | 6 |
| Bud Neck | 1 | 9 | 19 | 15 | 11 | 18 | 21 | 31 | 20 | 18 | 6 | 17 | 1 | 9 | 1 | 0 | – | 0 | 0 | 2 | 2 | 0 | 0 | 2 |
| Bud Site | 1 | 4 | 5 | 4 | 11 | 9 | 18 | 2 | 16 | 15 | 10 | 15 | 0 | 0 | 0 | 0 | – | 2 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 13 | 22 | 14 | 20 | 23 | 17 | 16 | 18 | 34 | 21 | 20 | 14 | 2 | 23 | 44 | 1 | – | 3 | 6 | 13 | 7 | 11 | 4 | 15 |
| Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
| Endosome | 14 | 59 | 16 | 7 | 0 | 8 | 3 | 5 | 3 | 4 | 7 | 2 | 2 | 16 | 43 | 0 | – | 3 | 10 | 41 | 22 | 16 | 9 | 9 |
| Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| Mitochondria | 21 | 61 | 4 | 7 | 15 | 17 | 30 | 18 | 62 | 31 | 51 | 64 | 0 | 1 | 0 | 3 | – | 1 | 0 | 11 | 0 | 1 | 0 | 4 |
| Nucleus | 1 | 3 | 4 | 2 | 3 | 5 | 6 | 6 | 8 | 7 | 12 | 8 | 0 | 5 | 3 | 0 | – | 0 | 0 | 0 | 0 | 11 | 7 | 8 |
| Nuclear Periphery | 2 | 0 | 0 | 2 | 2 | 1 | 5 | 6 | 9 | 6 | 3 | 8 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 1 | 2 | 0 |
| Nucleolus | 32 | 24 | 29 | 36 | 41 | 38 | 40 | 39 | 72 | 59 | 51 | 59 | 9 | 45 | 32 | 7 | – | 8 | 0 | 7 | 4 | 11 | 9 | 15 |
| Peroxisomes | 8 | 3 | 0 | 6 | 0 | 8 | 1 | 1 | 15 | 3 | 6 | 5 | 0 | 1 | 12 | 0 | – | 0 | 0 | 3 | 1 | 2 | 0 | 1 |
| SpindlePole | 70 | 222 | 170 | 133 | 118 | 158 | 194 | 197 | 176 | 188 | 93 | 144 | 15 | 106 | 88 | 12 | – | 11 | 98 | 295 | 215 | 135 | 139 | 160 |
| Vac/Vac Membrane | 2 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 2 | 1 | 0 | – | 0 | 0 | 1 | 0 | 3 | 1 | 4 |
| Unique Cell Count | 111 | 315 | 213 | 183 | 177 | 223 | 253 | 257 | 305 | 274 | 196 | 250 | 17 | 155 | 155 | 17 | 16 | 125 | 387 | 267 | 205 | 184 | 236 | |
| Labelled Cell Count | 165 | 427 | 282 | 242 | 243 | 298 | 366 | 351 | 455 | 385 | 273 | 361 | 29 | 210 | 224 | 26 | 29 | 125 | 387 | 267 | 205 | 184 | 236 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.9 | 3.8 | 5.8 | 5.5 | 4.5 | 5.2 | 4.8 | 5.5 | 4.5 | 4.9 | 5.3 | 4.4 | 4.8 | 5.5 | 5.4 | 6.0 | – | 6.9 | 6.6 | 7.1 | 6.9 |
| Std Deviation (1e-4) | 1.1 | 0.5 | 1.7 | 1.8 | 1.4 | 2.0 | 1.7 | 2.2 | 2.1 | 2.0 | 6.0 | 1.7 | 0.7 | 1.1 | 1.7 | 1.1 | – | 2.4 | 2.5 | 3.0 | 2.7 |
| Intensity Change (Log2) | – | – | – | -0.07 | -0.37 | -0.16 | -0.26 | -0.08 | -0.35 | -0.24 | -0.11 | -0.39 | -0.28 | -0.07 | -0.1 | 0.06 | – | 0.25 | 0.18 | 0.3 | 0.24 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
