Standard name
Human Ortholog
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0.05 0.07 0.05 0.1 0.08 0.11 0.1 0.11 0.11 0.07 0.1 0 0 0 0.06 – 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0.08 0.06 0.08 0.08 0.12 0.07 0.07 0 0.07 0.06 0.06 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0.06 0 0.07 0 0.05 0.05 0.05 0.06 0 0 0 0 – 0.12 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.12 0.07 0.07 0.11 0.13 0.08 0.06 0.07 0.11 0.08 0.1 0.06 0.12 0.15 0.28 0.06 – 0.19 0.05 0 0 0.05 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 – 0.06 0 0 0 0 0 0
Endosome 0.13 0.19 0.08 0 0 0 0 0 0 0 0 0 0.12 0.1 0.28 0 – 0.19 0.08 0.11 0.08 0.08 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.19 0.19 0 0 0.08 0.08 0.12 0.07 0.2 0.11 0.26 0.26 0 0 0 0.18 – 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.29 0.08 0.14 0.2 0.23 0.17 0.16 0.15 0.24 0.22 0.26 0.24 0.53 0.29 0.21 0.41 – 0.5 0 0 0 0.06 0.05 0.06
Peroxisomes 0.07 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.08 0 – 0 0 0 0 0 0 0
SpindlePole 0.63 0.7 0.8 0.73 0.67 0.71 0.77 0.77 0.58 0.69 0.47 0.58 0.88 0.68 0.57 0.71 – 0.69 0.79 0.76 0.81 0.66 0.76 0.68
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 0 1 0 3 2 4 2 0 0 0 0 0 0 – 0 0 1 0 0 0 2
Bud 0 16 14 9 17 17 29 25 34 29 13 24 0 2 0 1 – 0 3 1 1 2 5 6
Bud Neck 1 9 19 15 11 18 21 31 20 18 6 17 1 9 1 0 – 0 0 2 2 0 0 2
Bud Site 1 4 5 4 11 9 18 2 16 15 10 15 0 0 0 0 – 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 13 22 14 20 23 17 16 18 34 21 20 14 2 23 44 1 – 3 6 13 7 11 4 15
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 2 – 1 0 0 0 0 0 1
Endosome 14 59 16 7 0 8 3 5 3 4 7 2 2 16 43 0 – 3 10 41 22 16 9 9
Golgi 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0
Mitochondria 21 61 4 7 15 17 30 18 62 31 51 64 0 1 0 3 – 1 0 11 0 1 0 4
Nucleus 1 3 4 2 3 5 6 6 8 7 12 8 0 5 3 0 – 0 0 0 0 11 7 8
Nuclear Periphery 2 0 0 2 2 1 5 6 9 6 3 8 0 0 0 0 – 0 0 0 3 1 2 0
Nucleolus 32 24 29 36 41 38 40 39 72 59 51 59 9 45 32 7 – 8 0 7 4 11 9 15
Peroxisomes 8 3 0 6 0 8 1 1 15 3 6 5 0 1 12 0 – 0 0 3 1 2 0 1
SpindlePole 70 222 170 133 118 158 194 197 176 188 93 144 15 106 88 12 – 11 98 295 215 135 139 160
Vac/Vac Membrane 2 1 3 0 1 2 0 0 2 1 1 1 0 2 1 0 – 0 0 1 0 3 1 4
Unique Cell Count 111 315 213 183 177 223 253 257 305 274 196 250 17 155 155 17 16 125 387 267 205 184 236
Labelled Cell Count 165 427 282 242 243 298 366 351 455 385 273 361 29 210 224 26 29 125 387 267 205 184 236


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.8 5.8 5.5 4.5 5.2 4.8 5.5 4.5 4.9 5.3 4.4 4.8 5.5 5.4 6.0 – 6.9 6.6 7.1 6.9
Std Deviation (1e-4) 1.1 0.5 1.7 1.8 1.4 2.0 1.7 2.2 2.1 2.0 6.0 1.7 0.7 1.1 1.7 1.1 – 2.4 2.5 3.0 2.7
Intensity Change (Log2) – – – -0.07 -0.37 -0.16 -0.26 -0.08 -0.35 -0.24 -0.11 -0.39 -0.28 -0.07 -0.1 0.06 – 0.25 0.18 0.3 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4453 4.076 3.8103 3.7322 3.8368 3.528 5.0434 4.7839 5.7672 4.8642 3.744 5.169 2.9242 2.9923 3.1171 3.3592 3.6539 3.0519
Actin 0.0234 0.0013 0.0017 0.0006 0.0051 0.0006 0.0301 0.0006 0.0036 0.0256 0.0002 0.0096 0.0091 0.0004 0.0039 0.0253 0.0165 0.001
Bud 0.0001 0.0003 0.0002 0 0.0003 0 0.0009 0 0.0001 0.0002 0 0.0001 0.0002 0.0003 0.0003 0.0006 0.0001 0.0001
Bud Neck 0.0004 0.0013 0.0074 0.0005 0.0008 0.0005 0.0023 0.0002 0.0006 0.0007 0.0003 0.0002 0.0007 0.0026 0.0023 0.0021 0.0007 0.0013
Bud Periphery 0.0001 0.0005 0.0003 0 0.0002 0.0001 0.0019 0 0.0001 0.0003 0 0.0002 0.0002 0.0005 0.0004 0.0014 0.0001 0.0004
Bud Site 0.0003 0.0118 0.0052 0.0001 0.0016 0.0003 0.0016 0.0002 0.0011 0.0015 0.0003 0.0002 0.0021 0.0088 0.0019 0.0186 0.001 0.0005
Cell Periphery 0 0.001 0.0004 0 0.0001 0 0.0002 0 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0003 0 0
Cytoplasm 0.0014 0.0258 0.0013 0.0002 0.0116 0.0013 0.0063 0.0648 0.0022 0.0003 0.0002 0.0004 0.0022 0.0084 0.0032 0.0005 0.0032 0.0013
Cytoplasmic Foci 0.1619 0.1547 0.1425 0.1172 0.2362 0.1061 0.1169 0.1673 0.139 0.1302 0.1851 0.1035 0.0675 0.0572 0.0306 0.0925 0.1542 0.0491
Eisosomes 0.0001 0.0004 0 0 0 0 0.001 0 0.0001 0.0004 0 0 0.0002 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0006 0.0008 0.0001 0 0.0024 0.0001 0.0018 0.0011 0.0004 0.0002 0 0.0001 0.0006 0.0003 0.0006 0.0006 0.0005 0.0002
Endosome 0.017 0.0164 0.0117 0.0012 0.0873 0.0064 0.016 0.0029 0.0212 0.0082 0.0021 0.0058 0.0078 0.0036 0.0095 0.0072 0.0305 0.0059
Golgi 0.0114 0.0018 0.0039 0.0017 0.0157 0.009 0.0214 0.0013 0.0167 0.0161 0.0021 0.0108 0.0025 0.0014 0.0067 0.0019 0.0101 0.0017
Lipid Particles 0.0215 0.0175 0.0078 0.0095 0.1186 0.0297 0.0259 0.0047 0.0513 0.0743 0.0103 0.0061 0.0198 0.0064 0.0161 0.0038 0.0719 0.0107
Mitochondria 0.006 0.0078 0.0023 0 0.0091 0.0165 0.0041 0 0.0023 0.0219 0 0.0035 0.0021 0.0008 0.0039 0.0007 0.002 0.0006
None 0.0061 0.0055 0.005 0.0002 0.0029 0.0153 0.0682 0.0872 0.0195 0.0006 0.0003 0.0039 0.0115 0.0163 0.0044 0.0005 0.003 0.0025
Nuclear Periphery 0.0007 0.0019 0.0004 0.0006 0.0287 0.0019 0.0051 0.0003 0.0027 0.0005 0.0003 0.0006 0.0022 0.0024 0.0044 0.0019 0.0095 0.0035
Nucleolus 0.0009 0.0247 0.0047 0.0004 0.0014 0.0019 0.0039 0.0006 0.0043 0.0008 0.0011 0.0006 0.0035 0.0226 0.0187 0.003 0.0017 0.0101
Nucleus 0.0182 0.0226 0.0031 0.0002 0.0126 0.013 0.0044 0.0004 0.0015 0.0003 0.0004 0.0003 0.0022 0.0534 0.0152 0.0025 0.0174 0.0088
Peroxisomes 0.0418 0.018 0.0133 0.0127 0.041 0.008 0.0397 0.0212 0.0254 0.0937 0.0186 0.0151 0.0131 0.0118 0.0186 0.0086 0.072 0.0097
Punctate Nuclear 0.6879 0.6819 0.7876 0.8548 0.4196 0.7887 0.6466 0.647 0.7073 0.6233 0.7787 0.8387 0.8517 0.8017 0.8583 0.7864 0.603 0.8908
Vacuole 0.0002 0.0039 0.0008 0 0.003 0.0002 0.001 0.0001 0.0003 0.0003 0 0.0001 0.0003 0.0008 0.0005 0.0308 0.0015 0.0013
Vacuole Periphery 0.0002 0.0002 0.0003 0 0.002 0.0003 0.0007 0 0.0002 0.0003 0 0.0001 0.0002 0.0001 0.0002 0.0106 0.0011 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3414 7.9364 0.8413 5.4123 17.266 8.8731 6.3049 5.0291 6.26 11.3954
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
Localization
Cell Percentages spindle pole (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mtw1

Mtw1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtw1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available