TheCellVision

MTW1

Standard name	MTW1 ORF YAL034W-A Aliases DSN3, MIND complex subunit MTW1, NSL2
Human Ortholog	MIS12
Description	Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)

DOWNLOAD

Scale images

mtw1-ts

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0
Bud	0	0.05	0.07	0.05	0.1	0.08	0.11	0.1	0.11	0.11	0.07	0.1	0	0	0	0.06	–	0	0	0	0	0	0	0
Bud Neck	0	0	0.09	0.08	0.06	0.08	0.08	0.12	0.07	0.07	0	0.07	0.06	0.06	0	0	–	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0.06	0	0.07	0	0.05	0.05	0.05	0.06	0	0	0	0	–	0.12	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0
Cytoplasm	0.12	0.07	0.07	0.11	0.13	0.08	0.06	0.07	0.11	0.08	0.1	0.06	0.12	0.15	0.28	0.06	–	0.19	0.05	0	0	0.05	0	0.07
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.12	–	0.06	0	0	0	0	0	0
Endosome	0.13	0.19	0.08	0	0	0	0	0	0	0	0	0	0.12	0.1	0.28	0	–	0.19	0.08	0.11	0.08	0.08	0.05	0
Golgi	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0
Mitochondria	0.19	0.19	0	0	0.08	0.08	0.12	0.07	0.2	0.11	0.26	0.26	0	0	0	0.18	–	0.06	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	0	0	0.06	0	0	0	0	0	–	0	0	0	0	0.06	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0
Nucleolus	0.29	0.08	0.14	0.2	0.23	0.17	0.16	0.15	0.24	0.22	0.26	0.24	0.53	0.29	0.21	0.41	–	0.5	0	0	0	0.06	0.05	0.06
Peroxisomes	0.07	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0.08	0	–	0	0	0	0	0	0	0
SpindlePole	0.63	0.7	0.8	0.73	0.67	0.71	0.77	0.77	0.58	0.69	0.47	0.58	0.88	0.68	0.57	0.71	–	0.69	0.79	0.76	0.81	0.66	0.76	0.68
Vac/Vac Membrane	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	1	4	0	1	0	3	2	4	2	0	0	0	0	0	0	–	0	0	1	0	0	0	2
Bud	0	16	14	9	17	17	29	25	34	29	13	24	0	2	0	1	–	0	3	1	1	2	5	6
Bud Neck	1	9	19	15	11	18	21	31	20	18	6	17	1	9	1	0	–	0	0	2	2	0	0	2
Bud Site	1	4	5	4	11	9	18	2	16	15	10	15	0	0	0	0	–	2	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0
Cytoplasm	13	22	14	20	23	17	16	18	34	21	20	14	2	23	44	1	–	3	6	13	7	11	4	15
Endoplasmic Reticulum	0	2	0	0	0	0	0	0	0	1	0	0	0	0	0	2	–	1	0	0	0	0	0	1
Endosome	14	59	16	7	0	8	3	5	3	4	7	2	2	16	43	0	–	3	10	41	22	16	9	9
Golgi	0	0	0	1	0	0	0	1	0	0	0	0	0	0	0	0	–	0	0	0	1	0	0	0
Mitochondria	21	61	4	7	15	17	30	18	62	31	51	64	0	1	0	3	–	1	0	11	0	1	0	4
Nucleus	1	3	4	2	3	5	6	6	8	7	12	8	0	5	3	0	–	0	0	0	0	11	7	8
Nuclear Periphery	2	0	0	2	2	1	5	6	9	6	3	8	0	0	0	0	–	0	0	0	3	1	2	0
Nucleolus	32	24	29	36	41	38	40	39	72	59	51	59	9	45	32	7	–	8	0	7	4	11	9	15
Peroxisomes	8	3	0	6	0	8	1	1	15	3	6	5	0	1	12	0	–	0	0	3	1	2	0	1
SpindlePole	70	222	170	133	118	158	194	197	176	188	93	144	15	106	88	12	–	11	98	295	215	135	139	160
Vac/Vac Membrane	2	1	3	0	1	2	0	0	2	1	1	1	0	2	1	0	–	0	0	1	0	3	1	4
Unique Cell Count	111	315	213	183	177	223	253	257	305	274	196	250	17	155	155	17		16	125	387	267	205	184	236
Labelled Cell Count	165	427	282	242	243	298	366	351	455	385	273	361	29	210	224	26		29	125	387	267	205	184	236

Yeast GFP Assignment

SpindlePole

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	4.9	3.8	5.8	5.5	4.5	5.2	4.8	5.5	4.5	4.9	5.3	4.4	4.8	5.5	5.4	6.0	–	6.9	6.6	7.1	6.9
Std Deviation (1e^-4)	1.1	0.5	1.7	1.8	1.4	2.0	1.7	2.2	2.1	2.0	6.0	1.7	0.7	1.1	1.7	1.1	–	2.4	2.5	3.0	2.7
Intensity Change (Log₂)	–	–	–	-0.07	-0.37	-0.16	-0.26	-0.08	-0.35	-0.24	-0.11	-0.39	-0.28	-0.07	-0.1	0.06	–	0.25	0.18	0.3	0.24

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Actin	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud Neck	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Bud Site	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cell Periphery	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cyto	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endoplasmic Reticulum	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Endosome	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Golgi	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Mitochondria	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nuclear Periphery	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nuc	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleolus	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Peroxisomes	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
SpindlePole	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Vac	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cortical Patches	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Cytoplasm	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Nucleus	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Vacuole	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	3.4453	4.076	3.8103	3.7322	3.8368	3.528	5.0434	4.7839	5.7672	4.8642	3.744	5.169	2.9242	2.9923	3.1171	3.3592	3.6539	3.0519
Actin	0.0234	0.0013	0.0017	0.0006	0.0051	0.0006	0.0301	0.0006	0.0036	0.0256	0.0002	0.0096	0.0091	0.0004	0.0039	0.0253	0.0165	0.001
Bud	0.0001	0.0003	0.0002	0	0.0003	0	0.0009	0	0.0001	0.0002	0	0.0001	0.0002	0.0003	0.0003	0.0006	0.0001	0.0001
Bud Neck	0.0004	0.0013	0.0074	0.0005	0.0008	0.0005	0.0023	0.0002	0.0006	0.0007	0.0003	0.0002	0.0007	0.0026	0.0023	0.0021	0.0007	0.0013
Bud Periphery	0.0001	0.0005	0.0003	0	0.0002	0.0001	0.0019	0	0.0001	0.0003	0	0.0002	0.0002	0.0005	0.0004	0.0014	0.0001	0.0004
Bud Site	0.0003	0.0118	0.0052	0.0001	0.0016	0.0003	0.0016	0.0002	0.0011	0.0015	0.0003	0.0002	0.0021	0.0088	0.0019	0.0186	0.001	0.0005
Cell Periphery	0	0.001	0.0004	0	0.0001	0	0.0002	0	0	0.0001	0	0	0.0001	0.0001	0.0001	0.0003	0	0
Cytoplasm	0.0014	0.0258	0.0013	0.0002	0.0116	0.0013	0.0063	0.0648	0.0022	0.0003	0.0002	0.0004	0.0022	0.0084	0.0032	0.0005	0.0032	0.0013
Cytoplasmic Foci	0.1619	0.1547	0.1425	0.1172	0.2362	0.1061	0.1169	0.1673	0.139	0.1302	0.1851	0.1035	0.0675	0.0572	0.0306	0.0925	0.1542	0.0491
Eisosomes	0.0001	0.0004	0	0	0	0	0.001	0	0.0001	0.0004	0	0	0.0002	0.0001	0	0.0001	0.0001	0
Endoplasmic Reticulum	0.0006	0.0008	0.0001	0	0.0024	0.0001	0.0018	0.0011	0.0004	0.0002	0	0.0001	0.0006	0.0003	0.0006	0.0006	0.0005	0.0002
Endosome	0.017	0.0164	0.0117	0.0012	0.0873	0.0064	0.016	0.0029	0.0212	0.0082	0.0021	0.0058	0.0078	0.0036	0.0095	0.0072	0.0305	0.0059
Golgi	0.0114	0.0018	0.0039	0.0017	0.0157	0.009	0.0214	0.0013	0.0167	0.0161	0.0021	0.0108	0.0025	0.0014	0.0067	0.0019	0.0101	0.0017
Lipid Particles	0.0215	0.0175	0.0078	0.0095	0.1186	0.0297	0.0259	0.0047	0.0513	0.0743	0.0103	0.0061	0.0198	0.0064	0.0161	0.0038	0.0719	0.0107
Mitochondria	0.006	0.0078	0.0023	0	0.0091	0.0165	0.0041	0	0.0023	0.0219	0	0.0035	0.0021	0.0008	0.0039	0.0007	0.002	0.0006
None	0.0061	0.0055	0.005	0.0002	0.0029	0.0153	0.0682	0.0872	0.0195	0.0006	0.0003	0.0039	0.0115	0.0163	0.0044	0.0005	0.003	0.0025
Nuclear Periphery	0.0007	0.0019	0.0004	0.0006	0.0287	0.0019	0.0051	0.0003	0.0027	0.0005	0.0003	0.0006	0.0022	0.0024	0.0044	0.0019	0.0095	0.0035
Nucleolus	0.0009	0.0247	0.0047	0.0004	0.0014	0.0019	0.0039	0.0006	0.0043	0.0008	0.0011	0.0006	0.0035	0.0226	0.0187	0.003	0.0017	0.0101
Nucleus	0.0182	0.0226	0.0031	0.0002	0.0126	0.013	0.0044	0.0004	0.0015	0.0003	0.0004	0.0003	0.0022	0.0534	0.0152	0.0025	0.0174	0.0088
Peroxisomes	0.0418	0.018	0.0133	0.0127	0.041	0.008	0.0397	0.0212	0.0254	0.0937	0.0186	0.0151	0.0131	0.0118	0.0186	0.0086	0.072	0.0097
Punctate Nuclear	0.6879	0.6819	0.7876	0.8548	0.4196	0.7887	0.6466	0.647	0.7073	0.6233	0.7787	0.8387	0.8517	0.8017	0.8583	0.7864	0.603	0.8908
Vacuole	0.0002	0.0039	0.0008	0	0.003	0.0002	0.001	0.0001	0.0003	0.0003	0	0.0001	0.0003	0.0008	0.0005	0.0308	0.0015	0.0013
Vacuole Periphery	0.0002	0.0002	0.0003	0	0.002	0.0003	0.0007	0	0.0002	0.0003	0	0.0001	0.0002	0.0001	0.0002	0.0106	0.0011	0.0004

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	6.3414	7.9364	0.8413	5.4123	17.266	8.8731	6.3049	5.0291	6.26	11.3954
Translational Efficiency	–	–	–	–	–	–	–	–	–	–

Hit Data

Dataset	Hit
Protein Abundance	✘
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	–