Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.06 0 0 0 – 0 0 0 0 0 0.05 0 0 0.08 0 0 0.06 0.06 0 0 0.06 0.06
Bud 0 0 0 0 0 0 – 0 0.06 0.06 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0.11 0 0.11 0.12 0.08 0.08
Golgi 0.11 0.27 0 0 0 0 – 0 0 0 0 0 0.12 0.09 0.12 0.58 0.51 0.56 0.58 0.7 0.55 0.62 0.67 0.71
Mitochondria 0.93 0.92 0.96 0.92 0.88 0.9 – 0.73 0.86 0.87 0.82 0.84 0.95 0.92 0.88 0.07 0.08 0.08 0.08 0.08 0.09 0.09 0.09 0.06
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0 0 0.06 0 0.07 – 0.13 0.12 0.07 0.11 0.09 0 0 0 0.07 0.14 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0.11 0.14 0.16 – 0.19 0.08 0.16 0.15 0.17 0 0.08 0.1 0.08 0.21 0.08 0.13 0.07 0.2 0.1 0.07 0.05
SpindlePole 0 0 0 0 0 0 – 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 9 8 9 12 5 – 8 4 17 3 11 26 27 21 6 2 4 11 7 4 1 29 36
Bud 1 1 3 12 21 18 – 23 32 40 41 44 6 6 8 1 1 1 0 0 0 0 1 2
Bud Neck 0 0 0 1 0 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 3 – 4 5 18 4 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 – 0 2 0 2 3 2 0 1 1 1 0 0 0 0 0 0 0
Cytoplasm 0 1 0 7 8 7 – 9 4 18 13 10 13 17 21 5 4 8 0 0 0 0 1 2
Endoplasmic Reticulum 1 0 5 2 1 1 – 1 0 0 1 0 2 2 0 3 0 2 0 0 0 0 0 0
Endosome 7 1 0 2 20 14 – 29 5 2 4 4 6 24 28 5 3 2 21 5 15 13 42 48
Golgi 20 33 4 1 12 2 – 16 1 1 0 1 66 70 104 41 51 54 111 85 74 69 348 448
Mitochondria 175 111 130 310 427 434 – 543 423 545 370 549 499 683 765 5 8 8 15 9 12 10 48 36
Nucleus 1 0 0 0 2 0 – 5 2 2 2 1 0 0 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 5 0 3 1 3 7 – 11 11 6 10 11 8 6 5 1 0 3 0 0 0 0 0 1
Nucleolus 25 3 4 21 21 34 – 97 59 42 48 57 14 24 32 5 14 9 0 0 0 0 0 1
Peroxisomes 2 2 2 37 67 79 – 142 41 99 68 112 18 59 89 6 21 8 25 8 27 11 36 34
SpindlePole 1 0 0 5 12 11 – 35 21 28 25 27 1 2 4 3 4 4 3 0 0 0 1 1
Vac/Vac Membrane 3 5 4 5 5 1 – 11 5 2 4 5 21 11 26 5 5 6 2 3 0 3 6 10
Unique Cell Count 188 121 135 336 483 481 740 493 628 449 655 528 745 871 71 100 97 193 122 135 112 519 627
Labelled Cell Count 247 167 163 413 612 617 935 615 821 595 854 682 931 1104 87 114 109 193 122 135 112 519 627


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 13.1 8.9 8.9 9.8 9.5 – 10.2 9.1 8.6 8.5 8.7 12.9 14.0 13.8 15.7 17.0 16.0 10.6 12.5 13.1
Std Deviation (1e-4) 2.1 2.5 1.7 1.6 1.7 2.2 – 2.3 2.5 2.4 2.5 2.6 2.7 3.0 3.3 4.1 4.0 4.0 2.4 2.4 2.4
Intensity Change (Log2) – – – 0.0 0.13 0.09 – 0.19 0.04 -0.04 -0.07 -0.03 0.54 0.65 0.64 0.82 0.93 0.84 0.25 0.49 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 -0.5 -1.3 0 0 0 -0.6
Bud 0 1.1 0 – 0 1.9 1.9 2.7 2.0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 3.2 2.5 3.1 9.0 8.6 9.1
Mitochondria -1.6 -2.7 -2.2 – -5.8 -3.3 -3.1 -4.0 -3.8 -0.8 -1.9 -2.9 -12.8 -13.6 -13.5
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.4 0.7 1.8 – 3.4 3.1 1.6 2.8 2.3 0 0 0 0 3.1 2.1
Peroxisomes 3.4 4.0 4.5 – 5.1 2.8 4.4 4.3 4.7 0 2.7 3.3 0 5.0 0
SpindlePole 0 0 0 – 2.6 0 0 2.8 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0.6 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.8693 13.667 12.4424 11.5491 10.9191 13.3282 10.3568 12.6692 12.7521 12.0034 12.2834 12.7999 13.0089 15.4662 14.9417 12.9915 15.065 16.4676
Actin 0.0256 0.0387 0.0351 0.015 0.0158 0.0257 0.0895 0.0289 0.0321 0.0241 0.0414 0.0529 0.0914 0.049 0.0472 0.0185 0.0044 0.0088
Bud 0.0003 0.0003 0.0003 0.0003 0.0002 0.0001 0.0006 0.0003 0.0004 0.0001 0.0002 0.0001 0.0004 0.0029 0.0011 0.0002 0.0001 0.0005
Bud Neck 0.0024 0.0003 0.0002 0.0003 0.0003 0.0002 0.0008 0.0007 0.0005 0.0003 0.0003 0.0005 0.0005 0.0006 0.0015 0.0002 0.0001 0.0015
Bud Periphery 0.0004 0.0003 0.0003 0.0004 0.0002 0.0001 0.0009 0.0002 0.0005 0.0001 0.0002 0.0002 0.0006 0.0025 0.0014 0.0004 0.0001 0.0009
Bud Site 0.003 0.0008 0.0011 0.0017 0.0006 0.0001 0.0022 0.0044 0.0013 0.0002 0.0002 0.0002 0.002 0.0121 0.0184 0.0008 0.0001 0.001
Cell Periphery 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0005 0.0002 0.0002 0.0001 0.0003
Cytoplasm 0.002 0.0003 0.0004 0.0004 0.0122 0.0005 0.0009 0.0093 0.0115 0.0062 0.0004 0.0001 0.0072 0.0013 0.0005 0.0002 0.0001 0.0001
Cytoplasmic Foci 0.0621 0.0551 0.0474 0.0516 0.0585 0.0323 0.0457 0.0612 0.0424 0.0524 0.0377 0.0202 0.046 0.0456 0.0279 0.0151 0.0292 0.0174
Eisosomes 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0002 0.0005 0.0002 0.0004 0.0006 0.0001 0.0002
Endoplasmic Reticulum 0.0027 0.0001 0.0003 0.0009 0.0017 0.0006 0.0025 0.0002 0.0024 0.0048 0.0007 0.0002 0.0014 0.0003 0.0009 0.0003 0.0001 0.0001
Endosome 0.0948 0.0796 0.0711 0.0809 0.0741 0.0524 0.0673 0.1027 0.0913 0.1077 0.1238 0.0462 0.0752 0.112 0.0666 0.1101 0.0526 0.0343
Golgi 0.5992 0.7021 0.6386 0.7479 0.6661 0.7426 0.4551 0.6198 0.6031 0.4925 0.7065 0.6559 0.4394 0.5309 0.5952 0.4676 0.7225 0.6542
Lipid Particles 0.0754 0.0333 0.0926 0.0519 0.0287 0.0507 0.13 0.0853 0.0756 0.1895 0.0277 0.08 0.1137 0.0643 0.0747 0.0182 0.0382 0.1031
Mitochondria 0.0691 0.049 0.074 0.018 0.0898 0.0534 0.1022 0.0392 0.0785 0.0729 0.0361 0.0972 0.1113 0.1217 0.0957 0.3343 0.1392 0.1458
None 0.0019 0 0.0003 0.0004 0.0005 0.0003 0.0017 0.0001 0.0009 0.0002 0 0 0.0067 0.0001 0.0005 0.0005 0 0
Nuclear Periphery 0.005 0.0001 0.0008 0.0009 0.0045 0.0013 0.0228 0.0002 0.0011 0.0035 0.0002 0.0001 0.0159 0.0003 0.0008 0.0004 0.0001 0.0001
Nucleolus 0.0021 0.0004 0.0004 0.0002 0.0013 0.0005 0.0006 0.0021 0.0009 0.0004 0 0 0.0008 0.0011 0.0002 0.0005 0 0.0002
Nucleus 0.0012 0.0001 0.0003 0.0003 0.0032 0.0005 0.0016 0.0003 0.0003 0.0002 0 0 0.0088 0.0004 0.0002 0.0003 0 0.0001
Peroxisomes 0.0381 0.034 0.0314 0.0234 0.0219 0.0326 0.0578 0.0341 0.0402 0.0332 0.0205 0.0427 0.046 0.0264 0.0566 0.0109 0.0087 0.0249
Punctate Nuclear 0.0054 0.0001 0.001 0.0014 0.002 0.0034 0.0011 0.0004 0.0002 0.0004 0.0001 0.0001 0.0229 0.0002 0.0006 0.0001 0 0.0001
Vacuole 0.0042 0.0018 0.0014 0.0016 0.007 0.0006 0.0055 0.005 0.0086 0.0031 0.0011 0.0005 0.0024 0.0154 0.0017 0.004 0.0008 0.0015
Vacuole Periphery 0.0045 0.0033 0.0027 0.002 0.0111 0.002 0.0104 0.0054 0.0077 0.0078 0.0025 0.0023 0.0066 0.0124 0.0078 0.0165 0.0033 0.0047

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 103.4785 100.437 101.1112 108.9456 88.9684 88.8819 111.4753 103.4806 97.3105 109.5617
Translational Efficiency 0.989 1.0446 0.9238 0.8321 0.8836 0.9623 0.7823 0.6808 0.7526 0.6938

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘