Standard name
Description Chromatin associated high mobility group (HMG) family member; involved in compacting, bending, bridging and looping DNA; rDNA-binding component that regulates transcription from RNA polymerase I promoters; regulates start site selection of ribosomal protein genes via RNA polymerase II promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.07 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.37 0.33 0.27 0.25 0.21 0.24 0.22 0.15 0.33 0.2 0.28 0.29 0.25 0.21 0.2 0.07 0.06 0 0 0 0 0 0 0
Nucleus 0.13 0.09 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0.06 0.13 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.92 0.94 0.95 0.94 0.94 0.92 0.93 0.93 0.91 0.94 0.95 0.94 0.94 0.9 0.84 0.88 0.92 0.95 0.85 0.79 0.88 0.85 0.87 0.79
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0.07 0.09 0.06 0 0.06 0.05 0.08 0 0 0 0 0 0 0.05 0 0 0.06 0 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 6
Bud Neck 0 0 1 3 2 0 6 4 2 2 6 2 2 7 5 0 0 0 0 0 2 0 6 5
Bud Site 3 0 0 0 1 1 4 0 3 1 0 2 0 1 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 10 6 10 4 4 8 6 8 2 7 35 66 10 8 3 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 2 0 0 0 0 0 1
Endosome 0 0 0 0 1 4 1 2 1 0 0 2 0 1 7 1 1 1 2 0 0 2 3 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 86 18 47 60 57 75 98 82 143 69 113 139 70 115 120 10 10 3 1 0 0 2 4 7
Nucleus 30 5 11 7 7 6 10 17 10 8 8 13 13 14 13 8 6 5 13 9 6 3 14 5
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 1 2
Nucleolus 212 51 162 221 259 291 409 514 395 318 386 457 259 497 502 133 144 110 197 56 162 154 416 320
Peroxisomes 1 0 0 0 3 14 6 11 2 5 1 9 3 6 12 2 2 0 0 0 0 0 0 1
SpindlePole 2 0 3 9 14 22 38 32 19 22 21 40 4 21 9 1 2 0 11 2 5 10 19 32
Vac/Vac Membrane 0 0 0 1 0 2 2 5 3 1 2 0 0 3 3 1 0 1 2 0 2 2 5 9
Unique Cell Count 231 54 171 236 276 316 441 555 435 339 407 486 277 550 598 151 156 116 233 71 184 182 481 408
Labelled Cell Count 336 74 224 311 350 425 578 672 586 432 547 667 358 700 738 169 175 125 233 71 184 182 481 408


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.7 22.3 31.0 31.4 30.2 25.5 23.9 23.7 24.3 21.4 22.2 22.4 32.0 31.5 29.9 28.2 36.8 44.9 30.7 35.8 32.5
Std Deviation (1e-4) 14.5 10.4 11.9 14.2 12.1 11.3 9.9 8.9 11.7 8.3 9.7 9.6 14.7 14.7 16.0 13.7 18.2 21.7 12.9 15.4 14.4
Intensity Change (Log2) – – – 0.02 -0.04 -0.28 -0.38 -0.39 -0.35 -0.54 -0.48 -0.47 0.04 0.02 -0.06 -0.14 0.25 0.53 -0.02 0.21 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 3.4 4.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 -1.7 -0.9 -1.4 -3.8 1.3 -1.8 0.1 0.3 -0.5 -1.8 -2.1 -4.9 -5.0 -5.5
Nucleus -1.7 -2.0 -2.6 -2.5 -2.0 -2.5 -2.3 -2.7 -2.3 -0.8 -2.4 -2.8 -0.4 -1.1 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.5 -0.4 -1.1 -0.9 -1.0 -1.6 -0.4 0.1 -0.3 -0.5 -1.8 -3.6 -2.2 -0.9 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.2 1.8 2.5 3.0 2.1 1.5 2.3 1.9 2.9 0 1.3 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0