Standard name
Description Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – – 0 0 0 0 0 0
Bud 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0 0 0 0 0 0
Cytoplasm 0.08 0 0 0.06 0 0 0 0 0 0 0 0 0.09 0.08 0.05 0.05 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 – – 0 0 0 0 0 0
Endosome 0.08 0.13 0 0 0 0 0 0 0 0 0 0 0.1 0.16 0.16 0 – – 0.11 0.09 0.05 0.1 0.09 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.14 0.53 – – 0 0.05 0 0.07 0.1 0.09
Mitochondria 0.79 0.85 0.96 0.92 0.92 0.93 0.94 0.88 0.93 0.94 0.94 0.95 0.6 0.56 0.49 0 – – 0.73 0.72 0.79 0.6 0.59 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 0.12 0.08 0.11 0.13 0.07 0.08 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.09 0.14 0.06 0.09 0.07 0.08 0 0 0 0.11 – – 0 0 0 0 0 0
Peroxisomes 0.06 0.12 0 0.06 0 0 0 0 0 0 0 0 0.07 0.11 0.06 0 – – 0 0 0.09 0.07 0.06 0.08
SpindlePole 0 0 0 0.06 0 0 0 0 0 0 0 0 0.08 0.07 0.06 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.15 0 0 0.05 0 0 0.05 0 0 0 0 0.12 0.21 0.19 0.21 – – 0 0 0 0.05 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 3 0 0 0 0 0 1 0 0 0 9 1 5 1 – – 5 4 1 7 6 9
Bud 5 3 5 21 13 16 22 21 17 23 14 15 8 8 3 2 – – 0 1 0 0 2 4
Bud Neck 1 0 0 0 0 1 0 2 0 0 0 0 2 0 1 0 – – 1 1 0 3 1 4
Bud Site 1 0 1 1 0 12 5 5 7 6 7 1 0 0 0 0 – – – – – – – –
Cell Periphery 4 8 2 7 9 1 1 1 3 6 3 3 5 5 5 2 – – 0 0 0 0 0 1
Cytoplasm 22 6 5 15 14 19 8 20 12 10 10 6 18 25 14 1 – – 1 1 0 1 2 1
Endoplasmic Reticulum 6 0 6 1 0 2 3 1 1 4 1 3 9 4 23 1 – – 2 2 0 1 1 2
Endosome 22 31 0 0 11 1 1 2 0 2 0 1 21 48 47 0 – – 34 24 6 16 22 24
Golgi 2 1 0 0 0 0 0 1 1 0 0 1 15 16 41 10 – – 10 13 1 11 26 26
Mitochondria 217 199 141 248 432 454 478 610 393 504 326 488 122 164 147 0 – – 237 188 121 106 154 176
Nucleus 1 1 0 2 4 5 3 6 6 3 2 2 2 2 3 0 – – 0 0 0 0 0 1
Nuclear Periphery 3 1 5 2 15 31 63 58 47 71 23 40 0 1 2 0 – – 5 3 0 0 2 2
Nucleolus 0 2 1 3 19 34 48 96 26 51 25 41 0 1 0 2 – – 0 1 0 0 1 0
Peroxisomes 17 27 1 15 12 4 2 6 4 6 1 4 14 31 19 0 – – 12 9 14 11 16 23
SpindlePole 9 3 1 15 8 5 4 19 5 9 4 3 16 20 18 0 – – 5 1 1 0 2 1
Vac/Vac Membrane 46 36 3 5 23 21 22 34 17 16 9 16 25 63 57 4 – – 6 7 1 9 16 20
Unique Cell Count 275 234 147 270 469 490 506 693 422 539 345 513 203 295 301 19 326 263 153 176 260 307
Labelled Cell Count 357 318 174 335 560 606 660 882 540 711 425 624 266 389 385 23 326 263 153 176 260 307


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.8 4.1 3.9 4.9 4.9 4.9 5.3 5.5 5.6 5.7 6.3 6.2 6.2 6.4 10.8 – – 6.7 7.1 7.0
Std Deviation (1e-4) 0.8 1.8 0.6 1.2 1.2 1.2 1.5 1.6 1.7 1.8 2.0 2.2 2.7 1.6 1.2 2.6 – – 2.7 4.1 2.4
Intensity Change (Log2) – – – -0.07 0.26 0.28 0.28 0.37 0.43 0.45 0.49 0.62 0.61 0.6 0.66 1.41 – – 0.72 0.81 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 1.2 0 0 0 – –
Bud 0 0 0 0.5 0 0.3 0.5 0.3 0 0.3 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cytoplasm 1.0 0 0.3 0 0 0 0 0 0 2.0 2.0 0.6 0 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.2 0 1.4 0 – –
Endosome 0 0 0 0 0 0 0 0 0 4.0 5.2 5.1 0 – –
Golgi 0 0 0 0 0 0 0 0 0 3.4 2.9 4.7 0 – –
Mitochondria 0 0 -1.4 -0.7 -2.8 -1.2 -1.1 -0.7 -0.4 -7.7 -8.6 -9.8 -11.0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 1.3 3.2 2.1 2.8 3.3 1.4 1.9 0 0 0 0 – –
Nucleolus 0 0 2.9 3.6 4.5 2.7 3.6 3.0 3.2 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 2.8 3.8 2.7 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 3.1 2.8 2.6 0 – –
Vacuole 0 1.5 1.3 1.3 1.5 1.1 0 0 0 3.5 5.4 4.9 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7843 5.87 5.6472 5.7359 5.1801 5.4611 4.3722 5.3029 4.7242 4.7513 3.7643 4.6415 1.4373 2.2664 2.2987 1.7858 1.1283 2.0552
Actin 0.0294 0.0193 0.0103 0.0093 0.0127 0.0077 0.0318 0.0154 0.0122 0.0045 0.0314 0.0049 0.021 0.0206 0.0187 0.0019 0.0191 0.0146
Bud 0.0005 0.0011 0.0003 0.0059 0.0002 0.0001 0.0003 0.003 0.0003 0.0001 0.0003 0.0001 0.0005 0.0006 0.0006 0.0001 0.0005 0.0002
Bud Neck 0.0014 0.0002 0.0008 0.0002 0.0001 0.0001 0.0034 0.0001 0.004 0 0.0001 0.0001 0.0004 0.0003 0.0003 0.0001 0.0003 0.0002
Bud Periphery 0.0008 0.0013 0.0015 0.0186 0.0004 0.0001 0.0006 0.0045 0.0005 0.0002 0.0004 0.0001 0.0011 0.0011 0.0013 0.0001 0.001 0.0004
Bud Site 0.0021 0.0081 0.0014 0.0014 0.0002 0.0001 0.0017 0.0031 0.0016 0.0001 0.0005 0.0002 0.0012 0.0016 0.0013 0.0001 0.0006 0.0003
Cell Periphery 0.0002 0.0003 0.0003 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0005 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.0016 0.0008 0.0009 0.0005 0.0001 0.0001 0.0006 0.0002 0.0009 0.0001 0.0003 0.0001 0.0005 0.0003 0.0011 0.0001 0.0001 0.0003
Cytoplasmic Foci 0.0156 0.0092 0.0135 0.0032 0.0039 0.003 0.0167 0.0112 0.0098 0.0008 0.0171 0.0039 0.024 0.0139 0.0091 0.0402 0.008 0.0052
Eisosomes 0.0005 0.0006 0.0003 0.0003 0.0001 0.0001 0.0009 0.0002 0.0007 0.0001 0.0004 0.0002 0.0005 0.0003 0.0004 0.0002 0.0004 0.0003
Endoplasmic Reticulum 0.0009 0.0002 0.0003 0.0011 0.0001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0014 0.0017 0.0004 0.0001 0.0001 0.0003
Endosome 0.0364 0.0172 0.027 0.0149 0.0141 0.007 0.0119 0.0261 0.018 0.0113 0.0091 0.004 0.0351 0.0345 0.0333 0.0239 0.0118 0.0199
Golgi 0.0476 0.0593 0.0402 0.0106 0.021 0.0287 0.0327 0.0372 0.0217 0.0037 0.0303 0.0216 0.0595 0.0649 0.0284 0.0488 0.0364 0.0442
Lipid Particles 0.0133 0.0202 0.0095 0.0022 0.0161 0.0143 0.034 0.0066 0.0137 0.0015 0.0352 0.0066 0.0381 0.0317 0.0179 0.0258 0.1136 0.026
Mitochondria 0.7947 0.8199 0.8724 0.9181 0.9178 0.9261 0.7758 0.8412 0.8558 0.8983 0.8448 0.9206 0.7224 0.7672 0.8395 0.7584 0.7593 0.8468
None 0.0036 0.0003 0.0002 0.0008 0.0001 0.0001 0.0033 0.0001 0.0005 0 0.0004 0.0001 0.0015 0.0002 0.0003 0.0001 0.0001 0.0001
Nuclear Periphery 0.0085 0.0001 0.0003 0.0006 0.0001 0.0006 0.0007 0 0.0003 0.0001 0.0001 0.0001 0.0055 0.0051 0.0002 0 0.0001 0.0003
Nucleolus 0.0029 0.0031 0.0002 0.0002 0.0001 0 0.0005 0.0001 0.0002 0.0004 0.0001 0 0.0004 0.0003 0.0002 0 0.0001 0.0012
Nucleus 0.002 0.0004 0.0002 0.0004 0.0001 0.0001 0.0005 0 0.0001 0.0003 0 0 0.001 0.0007 0.0002 0 0.0001 0.0002
Peroxisomes 0.025 0.0343 0.0111 0.007 0.0106 0.0095 0.0809 0.0497 0.0547 0.0418 0.0269 0.0347 0.0778 0.0363 0.0334 0.0979 0.0449 0.0357
Punctate Nuclear 0.0075 0.0001 0.0001 0.0001 0.0001 0.0003 0.0012 0.0001 0.0002 0 0.0019 0.0001 0.0013 0.0001 0.0001 0.0009 0.0001 0.0001
Vacuole 0.0024 0.0017 0.0031 0.002 0.0008 0.0004 0.0008 0.0004 0.0018 0.0263 0.0002 0.0004 0.0031 0.0103 0.0069 0.0003 0.0008 0.0011
Vacuole Periphery 0.0031 0.0025 0.0063 0.0024 0.0011 0.0014 0.0012 0.0005 0.0024 0.0101 0.0005 0.0021 0.0036 0.0079 0.0061 0.0008 0.0024 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1775 18.0655 21.4486 31.5005 13.1008 16.1581 23.2892 24.6566 28.9588 26.2155
Translational Efficiency 5.1078 2.615 2.6107 3.1008 5.2446 7.5017 3.1263 3.5624 3.4774 2.3901

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘