Standard name | |
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Description | Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 9 | 1 | 5 | 1 | – | – | 5 | 4 | 1 | 7 | 6 | 9 |
Bud | 5 | 3 | 5 | 21 | 13 | 16 | 22 | 21 | 17 | 23 | 14 | 15 | 8 | 8 | 3 | 2 | – | – | 0 | 1 | 0 | 0 | 2 | 4 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | – | – | 1 | 1 | 0 | 3 | 1 | 4 |
Bud Site | 1 | 0 | 1 | 1 | 0 | 12 | 5 | 5 | 7 | 6 | 7 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 4 | 8 | 2 | 7 | 9 | 1 | 1 | 1 | 3 | 6 | 3 | 3 | 5 | 5 | 5 | 2 | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 22 | 6 | 5 | 15 | 14 | 19 | 8 | 20 | 12 | 10 | 10 | 6 | 18 | 25 | 14 | 1 | – | – | 1 | 1 | 0 | 1 | 2 | 1 |
Endoplasmic Reticulum | 6 | 0 | 6 | 1 | 0 | 2 | 3 | 1 | 1 | 4 | 1 | 3 | 9 | 4 | 23 | 1 | – | – | 2 | 2 | 0 | 1 | 1 | 2 |
Endosome | 22 | 31 | 0 | 0 | 11 | 1 | 1 | 2 | 0 | 2 | 0 | 1 | 21 | 48 | 47 | 0 | – | – | 34 | 24 | 6 | 16 | 22 | 24 |
Golgi | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 15 | 16 | 41 | 10 | – | – | 10 | 13 | 1 | 11 | 26 | 26 |
Mitochondria | 217 | 199 | 141 | 248 | 432 | 454 | 478 | 610 | 393 | 504 | 326 | 488 | 122 | 164 | 147 | 0 | – | – | 237 | 188 | 121 | 106 | 154 | 176 |
Nucleus | 1 | 1 | 0 | 2 | 4 | 5 | 3 | 6 | 6 | 3 | 2 | 2 | 2 | 2 | 3 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 1 | 5 | 2 | 15 | 31 | 63 | 58 | 47 | 71 | 23 | 40 | 0 | 1 | 2 | 0 | – | – | 5 | 3 | 0 | 0 | 2 | 2 |
Nucleolus | 0 | 2 | 1 | 3 | 19 | 34 | 48 | 96 | 26 | 51 | 25 | 41 | 0 | 1 | 0 | 2 | – | – | 0 | 1 | 0 | 0 | 1 | 0 |
Peroxisomes | 17 | 27 | 1 | 15 | 12 | 4 | 2 | 6 | 4 | 6 | 1 | 4 | 14 | 31 | 19 | 0 | – | – | 12 | 9 | 14 | 11 | 16 | 23 |
SpindlePole | 9 | 3 | 1 | 15 | 8 | 5 | 4 | 19 | 5 | 9 | 4 | 3 | 16 | 20 | 18 | 0 | – | – | 5 | 1 | 1 | 0 | 2 | 1 |
Vac/Vac Membrane | 46 | 36 | 3 | 5 | 23 | 21 | 22 | 34 | 17 | 16 | 9 | 16 | 25 | 63 | 57 | 4 | – | – | 6 | 7 | 1 | 9 | 16 | 20 |
Unique Cell Count | 275 | 234 | 147 | 270 | 469 | 490 | 506 | 693 | 422 | 539 | 345 | 513 | 203 | 295 | 301 | 19 | 326 | 263 | 153 | 176 | 260 | 307 | ||
Labelled Cell Count | 357 | 318 | 174 | 335 | 560 | 606 | 660 | 882 | 540 | 711 | 425 | 624 | 266 | 389 | 385 | 23 | 326 | 263 | 153 | 176 | 260 | 307 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.8 | 4.1 | 3.9 | 4.9 | 4.9 | 4.9 | 5.3 | 5.5 | 5.6 | 5.7 | 6.3 | 6.2 | 6.2 | 6.4 | 10.8 | – | – | 6.7 | 7.1 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.8 | 0.6 | 1.2 | 1.2 | 1.2 | 1.5 | 1.6 | 1.7 | 1.8 | 2.0 | 2.2 | 2.7 | 1.6 | 1.2 | 2.6 | – | – | 2.7 | 4.1 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.26 | 0.28 | 0.28 | 0.37 | 0.43 | 0.45 | 0.49 | 0.62 | 0.61 | 0.6 | 0.66 | 1.41 | – | – | 0.72 | 0.81 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 | – | – |
Bud | 0 | 0 | 0 | 0.5 | 0 | 0.3 | 0.5 | 0.3 | 0 | 0.3 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cytoplasm | 1.0 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.0 | 0.6 | 0 | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 1.4 | 0 | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 5.2 | 5.1 | 0 | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 2.9 | 4.7 | 0 | – | – |
Mitochondria | 0 | 0 | -1.4 | -0.7 | -2.8 | -1.2 | -1.1 | -0.7 | -0.4 | -7.7 | -8.6 | -9.8 | -11.0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 1.3 | 3.2 | 2.1 | 2.8 | 3.3 | 1.4 | 1.9 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 2.9 | 3.6 | 4.5 | 2.7 | 3.6 | 3.0 | 3.2 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.8 | 2.7 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 2.8 | 2.6 | 0 | – | – |
Vacuole | 0 | 1.5 | 1.3 | 1.3 | 1.5 | 1.1 | 0 | 0 | 0 | 3.5 | 5.4 | 4.9 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7843 | 5.87 | 5.6472 | 5.7359 | 5.1801 | 5.4611 | 4.3722 | 5.3029 | 4.7242 | 4.7513 | 3.7643 | 4.6415 | 1.4373 | 2.2664 | 2.2987 | 1.7858 | 1.1283 | 2.0552 |
Actin | 0.0294 | 0.0193 | 0.0103 | 0.0093 | 0.0127 | 0.0077 | 0.0318 | 0.0154 | 0.0122 | 0.0045 | 0.0314 | 0.0049 | 0.021 | 0.0206 | 0.0187 | 0.0019 | 0.0191 | 0.0146 |
Bud | 0.0005 | 0.0011 | 0.0003 | 0.0059 | 0.0002 | 0.0001 | 0.0003 | 0.003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0006 | 0.0006 | 0.0001 | 0.0005 | 0.0002 |
Bud Neck | 0.0014 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0034 | 0.0001 | 0.004 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 |
Bud Periphery | 0.0008 | 0.0013 | 0.0015 | 0.0186 | 0.0004 | 0.0001 | 0.0006 | 0.0045 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0011 | 0.0011 | 0.0013 | 0.0001 | 0.001 | 0.0004 |
Bud Site | 0.0021 | 0.0081 | 0.0014 | 0.0014 | 0.0002 | 0.0001 | 0.0017 | 0.0031 | 0.0016 | 0.0001 | 0.0005 | 0.0002 | 0.0012 | 0.0016 | 0.0013 | 0.0001 | 0.0006 | 0.0003 |
Cell Periphery | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0016 | 0.0008 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0003 | 0.0011 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0156 | 0.0092 | 0.0135 | 0.0032 | 0.0039 | 0.003 | 0.0167 | 0.0112 | 0.0098 | 0.0008 | 0.0171 | 0.0039 | 0.024 | 0.0139 | 0.0091 | 0.0402 | 0.008 | 0.0052 |
Eisosomes | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0003 |
Endoplasmic Reticulum | 0.0009 | 0.0002 | 0.0003 | 0.0011 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0017 | 0.0004 | 0.0001 | 0.0001 | 0.0003 |
Endosome | 0.0364 | 0.0172 | 0.027 | 0.0149 | 0.0141 | 0.007 | 0.0119 | 0.0261 | 0.018 | 0.0113 | 0.0091 | 0.004 | 0.0351 | 0.0345 | 0.0333 | 0.0239 | 0.0118 | 0.0199 |
Golgi | 0.0476 | 0.0593 | 0.0402 | 0.0106 | 0.021 | 0.0287 | 0.0327 | 0.0372 | 0.0217 | 0.0037 | 0.0303 | 0.0216 | 0.0595 | 0.0649 | 0.0284 | 0.0488 | 0.0364 | 0.0442 |
Lipid Particles | 0.0133 | 0.0202 | 0.0095 | 0.0022 | 0.0161 | 0.0143 | 0.034 | 0.0066 | 0.0137 | 0.0015 | 0.0352 | 0.0066 | 0.0381 | 0.0317 | 0.0179 | 0.0258 | 0.1136 | 0.026 |
Mitochondria | 0.7947 | 0.8199 | 0.8724 | 0.9181 | 0.9178 | 0.9261 | 0.7758 | 0.8412 | 0.8558 | 0.8983 | 0.8448 | 0.9206 | 0.7224 | 0.7672 | 0.8395 | 0.7584 | 0.7593 | 0.8468 |
None | 0.0036 | 0.0003 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0033 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0015 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0085 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0006 | 0.0007 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0055 | 0.0051 | 0.0002 | 0 | 0.0001 | 0.0003 |
Nucleolus | 0.0029 | 0.0031 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0004 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0012 |
Nucleus | 0.002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.001 | 0.0007 | 0.0002 | 0 | 0.0001 | 0.0002 |
Peroxisomes | 0.025 | 0.0343 | 0.0111 | 0.007 | 0.0106 | 0.0095 | 0.0809 | 0.0497 | 0.0547 | 0.0418 | 0.0269 | 0.0347 | 0.0778 | 0.0363 | 0.0334 | 0.0979 | 0.0449 | 0.0357 |
Punctate Nuclear | 0.0075 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0012 | 0.0001 | 0.0002 | 0 | 0.0019 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0001 |
Vacuole | 0.0024 | 0.0017 | 0.0031 | 0.002 | 0.0008 | 0.0004 | 0.0008 | 0.0004 | 0.0018 | 0.0263 | 0.0002 | 0.0004 | 0.0031 | 0.0103 | 0.0069 | 0.0003 | 0.0008 | 0.0011 |
Vacuole Periphery | 0.0031 | 0.0025 | 0.0063 | 0.0024 | 0.0011 | 0.0014 | 0.0012 | 0.0005 | 0.0024 | 0.0101 | 0.0005 | 0.0021 | 0.0036 | 0.0079 | 0.0061 | 0.0008 | 0.0024 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.1775 | 18.0655 | 21.4486 | 31.5005 | 13.1008 | 16.1581 | 23.2892 | 24.6566 | 28.9588 | 26.2155 |
Translational Efficiency | 5.1078 | 2.615 | 2.6107 | 3.1008 | 5.2446 | 7.5017 | 3.1263 | 3.5624 | 3.4774 | 2.3901 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |