Standard name
Human Ortholog
Description Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.94 0.91 0.91 0.81 0.64 0.62 0.57 0.62 0.58 0.98 0.99 0.99 0.77 0.73 0.8 0.95 0.94 0.85 0.91 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.1 0.35 0.12 0.38 0.54 0.57 0.63 0.63 0.62 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0.36 0.36 0.31 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2
Bud 1 0 5 0 0 1 5 6 3 7 11 1 1 0 1 1 0 1 0 0 2 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 3 0 4 0 0 0 0 0 0
Cell Periphery 0 1 0 1 1 2 1 2 2 3 1 0 0 2 5 2 3 0 0 1 0 1 0
Cytoplasm 179 308 212 154 157 180 225 175 186 234 252 352 425 390 288 211 235 170 300 195 238 255 250
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 2 2 3 2 0 14 5 6 0 0 1 0 1 1
Endosome 0 0 0 0 1 0 0 0 0 0 1 0 2 4 21 11 9 1 0 3 4 2 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 1 1 1 1
Mitochondria 4 8 23 60 21 85 190 160 204 239 272 0 1 1 4 10 2 1 4 7 3 4 2
Nucleus 0 1 2 0 2 4 12 5 4 11 12 0 1 3 2 8 4 0 3 0 1 1 4
Nuclear Periphery 0 0 0 0 0 1 1 6 3 2 7 0 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 1 0 0 0 0 2 0 1 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 1 0 0 4 0 1 1
Vac/Vac Membrane 3 7 1 4 2 6 17 15 11 12 18 2 0 8 134 105 90 2 5 2 5 4 6
Unique Cell Count 183 317 225 170 172 223 353 280 326 380 437 358 430 395 375 288 294 179 319 229 261 283 284
Labelled Cell Count 187 326 245 220 184 279 452 369 417 511 581 359 434 408 473 353 354 179 319 229 261 283 284


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.4 3.7 3.7 4.6 3.8 3.8 3.7 3.9 3.7 3.6 5.1 5.1 5.5 8.6 8.6 8.3 5.1 5.2 5.4
Std Deviation (1e-4) 1.3 0.8 0.9 1.2 1.2 0.8 1.2 1.5 1.0 1.1 0.8 0.8 0.8 5.4 1.9 2.4 2.2 1.2 1.1 1.6
Intensity Change (Log2) 0.01 0.3 0.04 0.04 0.0 0.06 0.01 -0.02 0.48 0.48 0.59 1.23 1.22 1.18 0.47 0.51 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -1.1 -4.3 -8.3 -8.4 -9.6 -8.8 -9.7 2.7 3.4 3.2 -5.5 -6.2 -4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -6.2 -6.5 -6.2 -5.2 -3.1 -5.0
Nucleus 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 3.1 0 0 2.7 0 0 0 10.0 10.0 9.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5661 1.1976 0.4207 0.4768 -0.4488 0.2381 0.3845 -0.0777 0.1612 -0.5505 0.2396 -0.0588 1.8523 1.3511 1.461 2.1013 1.0109 1.5233
Actin 0.0028 0.0003 0.002 0.0022 0.0272 0.0034 0.059 0.0037 0.0213 0.0345 0.0297 0.0068 0.0523 0.001 0.0031 0.0625 0.0009 0.0007
Bud 0.0003 0.0002 0.0009 0.0005 0.0007 0.0012 0.0017 0.0108 0.0015 0.0173 0.0008 0.0006 0.0033 0.0066 0.0052 0.0022 0.0001 0.0013
Bud Neck 0.0007 0.0027 0.0001 0.0001 0.0009 0.0027 0.0042 0.0002 0.0006 0.0004 0.0005 0.0006 0.004 0.0001 0.0002 0.0009 0.0002 0.0009
Bud Periphery 0.0013 0.0001 0.0013 0.0013 0.0013 0.0008 0.0031 0.0046 0.002 0.0079 0.0013 0.0005 0.0087 0.0022 0.001 0.0088 0.0001 0.0021
Bud Site 0.0004 0.0052 0.0016 0.0001 0.001 0.0011 0.0041 0.0076 0.0036 0.0068 0.0041 0.0001 0.0052 0.0058 0.004 0.0016 0 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0002 0.0001 0.0003 0.0011 0.0001 0.0006 0.0001 0.0001 0.0002 0 0.0001
Cytoplasm 0.2753 0.4428 0.3618 0.3548 0.4718 0.4492 0.1547 0.3191 0.2375 0.289 0.257 0.4248 0.2678 0.3411 0.3364 0.3263 0.416 0.373
Cytoplasmic Foci 0.012 0.0075 0.0145 0.0082 0.0129 0.0113 0.0177 0.0101 0.0233 0.0145 0.0414 0.0137 0.0143 0.0075 0.0122 0.0135 0.0085 0.0066
Eisosomes 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0001 0.0004 0.0001 0.0005 0.0001 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0022 0.0028 0.0023 0.0015 0.0042 0.0015 0.0092 0.0017 0.0023 0.0016 0.0033 0.0033 0.0037 0.0014 0.0018 0.0031 0.0014 0.0011
Endosome 0.0028 0.0033 0.0048 0.0037 0.0111 0.0042 0.0394 0.0034 0.022 0.0016 0.0407 0.0053 0.0164 0.0013 0.0044 0.01 0.0106 0.0016
Golgi 0.0004 0.0002 0.0008 0.0031 0.0023 0.0014 0.0152 0.0006 0.0123 0.0008 0.0162 0.0006 0.0064 0.0003 0.0011 0.0066 0.0003 0.0001
Lipid Particles 0.001 0.0002 0.0004 0.0095 0.0004 0.0004 0.0205 0.0004 0.0022 0.0003 0.0148 0.0005 0.0096 0.0002 0.0024 0.003 0.0003 0.0001
Mitochondria 0.0002 0.0003 0.0026 0.0097 0.0028 0.0006 0.0131 0.0054 0.0039 0.0014 0.0075 0.0005 0.0045 0.0006 0.0069 0.0057 0.0017 0.0004
None 0.6867 0.5247 0.5933 0.5926 0.4493 0.5071 0.5842 0.6238 0.6502 0.5911 0.5477 0.5347 0.5672 0.6229 0.5864 0.5363 0.5141 0.5964
Nuclear Periphery 0.0014 0.0022 0.0015 0.0011 0.0052 0.0015 0.0315 0.0006 0.004 0.0019 0.0011 0.0012 0.0078 0.0007 0.0036 0.0024 0.0252 0.001
Nucleolus 0.0002 0.0002 0.0003 0.0001 0.0002 0.0017 0.0013 0.0003 0.0003 0.0021 0.0015 0.0001 0.0011 0.0003 0.0011 0.0002 0.0005 0.0003
Nucleus 0.0051 0.0035 0.0045 0.0027 0.0032 0.006 0.0106 0.0029 0.0015 0.008 0.002 0.0037 0.0043 0.0021 0.013 0.0027 0.0123 0.0062
Peroxisomes 0.0005 0.0002 0.001 0.0049 0.0013 0.0003 0.0151 0.0004 0.0039 0.0027 0.0026 0.0008 0.0082 0.0007 0.0022 0.0086 0.0003 0.0004
Punctate Nuclear 0.0054 0.0019 0.0051 0.0022 0.0023 0.0046 0.0039 0.0032 0.0063 0.0164 0.0122 0.0017 0.0115 0.0045 0.0138 0.0026 0.0048 0.0069
Vacuole 0.0008 0.0015 0.0008 0.001 0.0009 0.0006 0.0065 0.0009 0.0008 0.001 0.0111 0.0005 0.0017 0.0005 0.0007 0.002 0.0013 0.0005
Vacuole Periphery 0.0001 0.0003 0.0002 0.0003 0.0009 0.0001 0.0035 0.0002 0.0005 0.0003 0.0028 0.0001 0.0008 0.0001 0.0004 0.0005 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.6411 10.0856 1.4968 4.3585 7.314 11.276 9.6877 9.6358 7.904 14.7556
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2529 1574 294 1626 1643 1724 2264 111 4172 3298 2558 1737

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.04 768.17 932.83 870.88 704.35 757.99 889.26 945.81 694.46 762.85 894.27 875.67
Standard Deviation 78.58 270.39 95.21 115.88 90.52 96.48 105.14 132.01 83.87 199.46 104.97 118.41
Intensity Change Log 2 0.158933 0.439122 0.339982 0.105886 0.336313 0.425258 0.132343 0.388031 0.383749

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000128 0.000693 0.000377 0.003975 0.000092 0.000582 0.000180 0.000371 0.000114 0.000635 0.000203 0.003745
Bud Neck 0.014271 0.037195 0.001950 0.012548 0.009693 0.039551 0.011906 0.001416 0.012468 0.038427 0.010762 0.011836
Bud Site 0.003467 0.018435 0.001691 0.050598 0.002252 0.020215 0.011680 0.003531 0.002988 0.019365 0.010532 0.047590
Cell Periphery 0.000092 0.000214 0.000162 0.000180 0.000081 0.000155 0.000092 0.000141 0.000088 0.000183 0.000100 0.000178
Cytoplasm 0.547868* 0.436530* 0.202079* 0.467785* 0.547062* 0.430258* 0.392688* 0.381153* 0.547551* 0.433252* 0.370781* 0.462249*
Cytoplasmic Foci 0.144468 0.198677* 0.010057 0.017094 0.159166 0.219637* 0.005093 0.020908 0.150257 0.209634* 0.005663 0.017338
Eisosomes 0.000105 0.000086 0.000070 0.000043 0.000095 0.000128 0.000021 0.000065 0.000101 0.000108 0.000027 0.000045
Endoplasmic Reticulum 0.000878 0.003654 0.005797 0.002419 0.000521 0.000682 0.001796 0.006490 0.000738 0.002100 0.002256 0.002679
Endosome 0.006181 0.023611 0.003992 0.011725 0.004029 0.022804 0.001122 0.003227 0.005334 0.023189 0.001451 0.011181
Golgi 0.001456 0.005254 0.000089 0.005657 0.000907 0.006571 0.000063 0.000081 0.001240 0.005942 0.000066 0.005301
Lipid Particles 0.005501 0.004889 0.002700 0.002330 0.006121 0.005529 0.000597 0.001814 0.005745 0.005224 0.000839 0.002297
Mitochondria 0.002257 0.012873 0.000415 0.005289 0.004065 0.014882 0.001765 0.000291 0.002969 0.013923 0.001610 0.004969
Mitotic Spindle 0.000667 0.013023 0.001406 0.065674 0.001402 0.013263 0.011741 0.002643 0.000956 0.013148 0.010553 0.061646
None 0.006185 0.004308 0.003408 0.004655 0.001901 0.003207 0.005477 0.002869 0.004498 0.003732 0.005239 0.004541
Nuclear Periphery 0.000319 0.000780 0.002162 0.001768 0.000485 0.000476 0.002055 0.001757 0.000384 0.000621 0.002067 0.001767
Nuclear Periphery Foci 0.000309 0.000483 0.002187 0.001117 0.000210 0.000265 0.000422 0.003766 0.000270 0.000369 0.000625 0.001286
Nucleolus 0.001091 0.002104 0.000680 0.000924 0.000749 0.002296 0.000342 0.000281 0.000956 0.002204 0.000381 0.000883
Nucleus 0.219095* 0.123515 0.693170* 0.215631* 0.222093* 0.118022 0.511395* 0.387946* 0.220275* 0.120643 0.532287* 0.226642*
Peroxisomes 0.002392 0.015079 0.000289 0.003805 0.002500 0.016060 0.000313 0.000242 0.002434 0.015592 0.000310 0.003577
Vacuole 0.042714 0.095326 0.067121 0.120372 0.036177 0.082550 0.040973 0.180866 0.040140 0.088648 0.043979 0.124238
Vacuole Periphery 0.000557 0.003275 0.000199 0.006413 0.000400 0.002867 0.000278 0.000142 0.000495 0.003062 0.000269 0.006012

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.30 -5.49 -3.25 -2.07 -2.44 -3.32 -4.50 -5.51 -0.39 -4.18 -4.58 -4.24 -3.36 -2.23 -3.03
Bud Neck -9.97 13.37 3.83 11.86 -7.29 -13.09 0.35 8.39 16.79 6.34 -15.94 3.86 3.20 16.78 -0.60
Bud Site -10.33 1.80 -9.02 -2.34 -9.47 -11.49 -5.33 -2.77 7.43 1.40 -15.28 -4.73 -9.35 -1.65 -6.81
Cell Periphery -2.03 -5.94 -3.01 1.67 4.22 -4.62 -0.73 -5.13 -0.29 -4.99 -3.17 -2.16 -4.19 2.18 -2.61
Cytoplasm 13.17 31.17 20.40 5.47 -15.54 12.70 29.83 10.35 5.21 -0.10 18.53 41.47 23.02 7.02 -9.87
Cytoplasmic Foci -8.42 37.56 37.62 34.97 -2.30 -8.90 35.77 28.74 35.83 -4.62 -12.82 53.63 49.19 52.44 -8.49
Eisosomes 3.51 1.43 12.33 9.37 6.07 -3.96 9.90 -0.37 2.61 -7.08 -1.45 16.63 12.16 14.80 -6.43
Endoplasmic Reticulum -2.39 -11.35 -10.87 0.55 8.45 -1.16 -18.81 -7.08 -6.92 -4.58 -2.43 -19.15 -15.07 -2.28 3.40
Endosome -12.00 2.29 1.32 11.99 -0.48 -14.26 11.18 -0.81 12.29 -4.95 -18.12 13.26 0.32 15.94 -5.81
Golgi -6.19 9.13 -0.49 4.21 -3.00 -6.96 5.51 4.92 8.07 -3.80 -9.07 10.88 -0.76 5.86 -3.09
Lipid Particles 1.18 4.56 7.06 6.19 3.03 0.67 6.86 3.41 3.73 -6.24 1.11 11.55 7.92 7.88 -3.71
Mitochondria -7.14 7.53 -0.79 6.82 -6.42 -5.95 2.87 4.32 9.14 7.85 -9.49 3.92 1.11 10.13 -2.95
Mitotic Spindle -6.62 -0.97 -12.62 -8.80 -10.08 -5.88 -6.50 -1.64 0.04 -0.18 -8.89 -7.18 -12.64 -8.95 -9.52
None 1.96 4.92 2.05 -0.73 -4.96 -2.01 -14.28 -0.92 1.01 5.51 1.28 -1.52 -0.32 -1.68 1.57
Nuclear Periphery -4.77 -19.01 -19.89 -14.83 -4.89 0.08 -28.67 -3.96 -3.96 -0.64 -3.83 -35.40 -19.78 -17.31 -3.43
Nuclear Periphery Foci -2.86 -7.69 -10.50 -8.10 3.81 -1.28 -7.22 -2.97 -2.96 -2.89 -2.76 -9.95 -6.01 -5.70 -4.27
Nucleolus -4.28 3.51 -0.97 2.95 -2.97 -3.53 4.31 0.70 3.57 -1.04 -4.87 8.53 -1.58 3.31 -4.86
Nucleus 16.82 -28.64 -6.34 -18.38 24.53 17.54 -46.66 -4.38 -8.70 9.91 24.75 -58.28 -7.26 -22.09 38.64
Peroxisomes -11.04 9.70 1.59 10.98 -2.50 -10.97 11.45 10.23 12.91 -2.00 -15.51 14.26 2.04 14.81 -2.77
Vacuole -15.04 -7.03 -28.48 -17.42 -10.28 -13.84 -10.78 -10.53 -8.42 -8.96 -20.10 -12.21 -30.75 -21.23 -23.10
Vacuole Periphery -3.94 8.32 -2.32 1.17 -2.83 -6.75 4.89 7.39 7.46 1.18 -6.81 8.27 -2.38 1.48 -2.88
Standard name
Human Ortholog
Description Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
Localization
Cell Percentages cytoplasm (30%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Csn9

Csn9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Csn9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available