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Description | Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 0 | 2 | 7 | 4 | 7 | 14 | 4 | 3 | 5 | 5 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 1 | 4 | 8 | 8 | 18 | 27 | 4 | 8 | 10 | 15 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 9 | 3 | 9 | 13 | 19 | 38 | 38 | 16 | 26 | 10 | 19 | 21 | 46 | 54 | 6 | 6 | 4 | 0 | 2 | 2 | 0 | 3 | 4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 5 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 20 | 61 | 79 | 138 | 111 | 150 | 245 | 301 | 176 | 246 | 142 | 194 | 17 | 18 | 49 | 6 | 2 | 2 | 0 | 4 | 4 | 0 | 4 | 9 |
Nucleus | 216 | 301 | 116 | 157 | 183 | 158 | 237 | 255 | 108 | 174 | 99 | 136 | 177 | 276 | 252 | 78 | 49 | 49 | 218 | 296 | 112 | 145 | 157 | 170 |
Nuclear Periphery | 1 | 2 | 2 | 1 | 4 | 0 | 3 | 1 | 0 | 8 | 0 | 1 | 2 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 |
Nucleolus | 8 | 0 | 1 | 2 | 8 | 11 | 14 | 21 | 9 | 9 | 11 | 9 | 3 | 10 | 8 | 4 | 0 | 1 | 6 | 3 | 3 | 2 | 4 | 14 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 4 | 2 | 2 | 11 | 2 | 0 | 1 | 2 | 0 | 4 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 4 |
Vac/Vac Membrane | 4 | 13 | 1 | 1 | 8 | 4 | 3 | 5 | 1 | 13 | 3 | 8 | 1 | 12 | 11 | 4 | 5 | 6 | 7 | 25 | 16 | 17 | 62 | 91 |
Unique Cell Count | 225 | 339 | 142 | 200 | 252 | 235 | 347 | 411 | 206 | 303 | 173 | 230 | 204 | 341 | 331 | 95 | 58 | 58 | 242 | 347 | 149 | 170 | 250 | 323 |
Labelled Cell Count | 253 | 387 | 204 | 314 | 348 | 357 | 567 | 676 | 320 | 487 | 281 | 389 | 221 | 371 | 387 | 103 | 64 | 63 | 242 | 347 | 149 | 170 | 250 | 323 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.7 | 3.6 | 3.6 | 3.9 | 3.7 | 3.4 | 3.4 | 2.9 | 3.0 | 3.1 | 2.8 | 5.3 | 5.4 | 4.7 | 8.2 | 8.8 | 9.6 | 5.2 | 5.2 | 5.4 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.0 | 1.0 | 1.3 | 1.6 | 1.4 | 1.6 | 1.2 | 1.2 | 1.6 | 0.7 | 1.4 | 1.3 | 1.5 | 2.2 | 2.3 | 2.2 | 1.2 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.12 | 0.04 | -0.11 | -0.07 | -0.31 | -0.25 | -0.2 | -0.37 | 0.56 | 0.58 | 0.37 | 1.18 | 1.29 | 1.42 | 0.52 | 0.52 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |