| Standard name | |
|---|---|
| Description | Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 2 | 8 |
| Bud | 0 | 0 | 1 | 2 | 4 | 3 | 0 | 8 | 6 | 6 | 9 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 8 | 15 |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 4 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 3 | 0 | 0 | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 1 | 2 |
| Cytoplasm | 162 | 112 | 161 | 118 | 162 | 223 | 209 | 316 | 173 | 209 | 129 | 178 | 290 | 473 | 503 | 203 | 179 | 126 | 136 | 105 | 107 | 225 | 301 | 328 |
| Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 13 | 18 | 3 | 1 | 0 | 3 | 0 | 1 | 3 |
| Endosome | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 3 | 4 | 4 | 1 | 5 | 3 | 3 | 5 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
| Mitochondria | 47 | 5 | 2 | 10 | 12 | 65 | 72 | 64 | 69 | 97 | 100 | 111 | 0 | 0 | 0 | 12 | 1 | 3 | 28 | 9 | 25 | 12 | 20 | 18 |
| Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
| Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 4 | 1 | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 6 | 3 |
| Vac/Vac Membrane | 0 | 0 | 1 | 1 | 2 | 4 | 2 | 3 | 4 | 2 | 0 | 2 | 0 | 2 | 0 | 32 | 22 | 14 | 14 | 7 | 7 | 6 | 11 | 36 |
| Unique Cell Count | 183 | 114 | 163 | 124 | 174 | 247 | 243 | 361 | 213 | 261 | 188 | 235 | 290 | 475 | 506 | 233 | 196 | 140 | 201 | 135 | 162 | 257 | 371 | 438 |
| Labelled Cell Count | 210 | 118 | 166 | 133 | 187 | 297 | 286 | 405 | 260 | 332 | 243 | 302 | 295 | 479 | 508 | 273 | 226 | 151 | 201 | 135 | 162 | 257 | 371 | 438 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.3 | 4.0 | 3.8 | 3.5 | 4.0 | 3.1 | 2.8 | 3.3 | 3.0 | 2.9 | 2.8 | 2.6 | 4.1 | 3.9 | 4.0 | 5.1 | 6.5 | 5.8 | 3.8 | 4.2 | 4.9 |
| Std Deviation (1e-4) | 0.8 | 1.0 | 1.6 | 1.6 | 1.9 | 1.2 | 0.9 | 1.3 | 1.7 | 1.7 | 1.0 | 1.0 | 1.0 | 1.0 | 1.1 | 2.0 | 3.4 | 1.8 | 0.9 | 1.5 | 1.3 |
| Intensity Change (Log2) | – | – | – | -0.09 | 0.1 | -0.26 | -0.44 | -0.2 | -0.31 | -0.39 | -0.42 | -0.54 | 0.13 | 0.06 | 0.11 | 0.45 | 0.8 | 0.62 | 0.02 | 0.16 | 0.37 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.6 | 4.1 | 3.8 |
External localization resources
