Standard name
Description Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.98 0.99 0.95 0.93 0.9 0.86 0.88 0.81 0.8 0.69 0.76 1.0 1.0 0.99 0.87 0.91 0.9 0.68 0.78 0.67 0.88 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0 0 0.08 0.07 0.26 0.3 0.18 0.32 0.37 0.53 0.47 0 0 0 0.05 0 0 0.14 0.07 0.16 0.05 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.11 0.1 0.07 0.05 0.05 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 1 1 0 2 8
Bud 0 0 1 2 4 3 0 8 6 6 9 3 1 2 0 0 0 0 1 0 1 2 8 15
Bud Neck 0 0 0 0 0 0 0 2 3 3 1 3 0 0 0 0 0 0 2 0 0 1 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 1 0 0 1 2 0 0 2 3 0 0 2 3 0 0 2 0 0 1 2
Cytoplasm 162 112 161 118 162 223 209 316 173 209 129 178 290 473 503 203 179 126 136 105 107 225 301 328
Endoplasmic Reticulum 0 0 0 1 0 0 1 1 1 0 1 0 0 0 2 13 18 3 1 0 3 0 1 3
Endosome 0 0 0 0 4 0 0 0 0 0 0 0 0 1 0 7 3 4 4 1 5 3 3 5
Golgi 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 1 1
Mitochondria 47 5 2 10 12 65 72 64 69 97 100 111 0 0 0 12 1 3 28 9 25 12 20 18
Nucleus 0 0 0 0 2 0 0 3 1 2 0 1 1 0 1 3 0 1 0 1 0 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 1 2 4 1 9 3 0 0 0 0 0 0 0 0 1 2 1 6 3
Vac/Vac Membrane 0 0 1 1 2 4 2 3 4 2 0 2 0 2 0 32 22 14 14 7 7 6 11 36
Unique Cell Count 183 114 163 124 174 247 243 361 213 261 188 235 290 475 506 233 196 140 201 135 162 257 371 438
Labelled Cell Count 210 118 166 133 187 297 286 405 260 332 243 302 295 479 508 273 226 151 201 135 162 257 371 438


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 3.8 3.5 4.0 3.1 2.8 3.3 3.0 2.9 2.8 2.6 4.1 3.9 4.0 5.1 6.5 5.8 3.8 4.2 4.9
Std Deviation (1e-4) 0.8 1.0 1.6 1.6 1.9 1.2 0.9 1.3 1.7 1.7 1.0 1.0 1.0 1.0 1.1 2.0 3.4 1.8 0.9 1.5 1.3
Intensity Change (Log2) – – – -0.09 0.1 -0.26 -0.44 -0.2 -0.31 -0.39 -0.42 -0.54 0.13 0.06 0.11 0.45 0.8 0.62 0.02 0.16 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.6 4.1 3.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available