Standard name
Human Ortholog
Description Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.47 0.38 0.53 0.76 0.98 0.95 0.93 0.93 0.76 0.81 0.73 0.77 0.79 1.0 1.0 – – 0.54 0.25 0.29 0.25 0.23 0.81 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0.07 0 0 – – 0.11 0.08 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.12 0.52 0 0.1 0 0.05 0.1 0.07 0.34 0.23 0.37 0.33 0 0 0 – – 0.08 0.11 0.07 0.11 0 0 0
Nucleus 0.12 0.11 0.23 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.16 0.17 0.18 0.18 0.26 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.11 0.13 0.12 0.18 0.05 0.07
Vac/Vac Membrane 0.35 0.13 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 – – 0.21 0.17 0.19 0.16 0.14 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 3 0 0 0 2
Bud 0 0 1 0 0 2 0 1 0 2 5 3 0 0 0 – – 0 1 8 2 2 4 9
Bud Neck 0 1 4 1 4 9 3 14 3 12 7 6 1 0 1 – – 0 1 3 0 3 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 – – 0 – – – – – –
Cell Periphery 0 1 0 0 2 1 2 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 8 81 52 76 199 278 342 380 210 242 273 291 22 269 250 – – 79 28 82 50 34 235 213
Endoplasmic Reticulum 0 3 2 0 0 0 1 0 0 0 1 0 0 0 0 – – 5 0 1 1 0 0 0
Endosome 0 0 5 5 0 0 0 1 0 0 1 0 2 0 0 – – 16 9 8 5 5 4 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – 2 0 1 0 0 2 0
Mitochondria 2 110 3 10 1 14 38 27 94 67 140 124 1 0 1 – – 11 12 19 22 4 6 6
Nucleus 2 24 23 3 1 4 4 0 1 1 2 2 1 0 0 – – 24 19 50 37 40 2 7
Nuclear Periphery 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 – – 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 4 4 5 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 1 0 0 0 0 0 0
SpindlePole 0 6 6 1 2 0 3 7 1 6 3 14 1 0 0 – – 5 12 36 25 27 13 20
Vac/Vac Membrane 6 27 9 10 4 1 1 3 5 4 3 2 0 4 0 – – 31 18 53 33 22 11 9
Unique Cell Count 17 213 98 100 204 292 366 409 276 297 376 379 28 270 251 146 113 287 206 154 291 290
Labelled Cell Count 18 254 105 106 213 309 394 433 316 335 437 444 28 273 253 174 113 287 206 154 291 290


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.3 5.1 4.7 4.8 4.0 3.9 4.0 3.2 3.5 3.3 3.4 4.6 4.5 4.4 4.1 4.9 5.3 5.4 5.1 5.3
Std Deviation (1e-4) 0.4 0.5 1.3 1.9 1.4 1.7 1.4 1.0 1.2 1.0 1.0 0.9 0.7 1.0 1.2 1.2 1.7 1.8 1.8 1.6 1.9
Intensity Change (Log2) – – – -0.1 -0.08 -0.34 -0.36 -0.33 -0.67 -0.53 -0.6 -0.59 -0.14 -0.18 -0.21 -0.32 -0.06 0.06 0.1 0.02 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5597 3.0939 3.5211 2.7424 2.7973 3.2307 1.7164 1.7271 1.6402 1.5455 1.654 1.5828 -1.3332 -0.9668 -1.199 -1.2311 -1.0509 -1.3406
Actin 0.0623 0.0016 0.0186 0.0012 0.0098 0.0019 0.0256 0.0004 0.009 0.0132 0.0122 0.0099 0.0286 0.0014 0.0095 0.0003 0.0042 0.007
Bud 0.001 0.0005 0.0005 0.0001 0.0003 0.0006 0.0009 0.0001 0.0034 0.0016 0.0009 0.0002 0.0007 0.0004 0.0002 0.0001 0.0002 0.0011
Bud Neck 0.0126 0.0013 0.0009 0.0005 0.0018 0.0074 0.0083 0.0005 0.0008 0.0024 0.001 0.0027 0.0015 0.0024 0.0062 0.001 0.0008 0.0031
Bud Periphery 0.0017 0.0004 0.0011 0.0006 0.0006 0.0051 0.0015 0.0001 0.0069 0.004 0.001 0.0005 0.0009 0.0002 0.0002 0.0001 0.0002 0.0024
Bud Site 0.0153 0.0088 0.0072 0.0001 0.0015 0.0003 0.0105 0.0028 0.0016 0.0098 0.0094 0.0003 0.0025 0.0049 0.0043 0.0002 0.0004 0.0005
Cell Periphery 0.0006 0.0002 0.001 0.0001 0.0006 0.0002 0.0003 0.0001 0.0002 0.0018 0.0003 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.1701 0.2565 0.1547 0.2276 0.1677 0.219 0.0712 0.2181 0.1673 0.1225 0.1683 0.2019 0.2141 0.2558 0.2056 0.1971 0.2062 0.2932
Cytoplasmic Foci 0.0539 0.0149 0.0147 0.007 0.0203 0.0166 0.049 0.0057 0.0066 0.06 0.0232 0.0125 0.0364 0.0132 0.018 0.0087 0.0109 0.0102
Eisosomes 0.0005 0.0002 0.0007 0.0002 0.0006 0.0001 0.0006 0.0002 0.0003 0.0002 0.0003 0.0003 0.0003 0.0001 0.0002 0 0.0001 0.0002
Endoplasmic Reticulum 0.0109 0.0036 0.0076 0.0035 0.0039 0.0033 0.0059 0.0037 0.0102 0.0016 0.0026 0.0079 0.0092 0.0073 0.0065 0.003 0.0094 0.0035
Endosome 0.0335 0.0025 0.0119 0.0036 0.0246 0.0048 0.0354 0.004 0.0102 0.025 0.025 0.0087 0.0231 0.0076 0.0078 0.0046 0.0312 0.0057
Golgi 0.0148 0.0004 0.0053 0.0001 0.0272 0.0004 0.0075 0.0002 0.0019 0.0046 0.0181 0.0136 0.0062 0.0003 0.0019 0.0001 0.008 0.0012
Lipid Particles 0.0245 0.0015 0.0063 0.0004 0.0477 0.001 0.0245 0.0008 0.0016 0.0103 0.004 0.0026 0.0163 0.0023 0.003 0.0002 0.0105 0.0012
Mitochondria 0.0063 0.0065 0.0251 0.0013 0.0081 0.0017 0.0194 0.0005 0.0191 0.0061 0.0988 0.0418 0.0044 0.0006 0.0011 0.001 0.0008 0.002
None 0.3443 0.3607 0.4068 0.456 0.4453 0.3858 0.5857 0.5272 0.4367 0.564 0.4702 0.3362 0.3877 0.4177 0.343 0.3014 0.4983 0.2699
Nuclear Periphery 0.0213 0.0088 0.0253 0.017 0.0184 0.0088 0.0308 0.0077 0.0094 0.0028 0.0082 0.0076 0.0326 0.0189 0.0269 0.0137 0.0183 0.0105
Nucleolus 0.0033 0.0066 0.0044 0.0024 0.0028 0.006 0.004 0.0031 0.0034 0.0027 0.0012 0.0036 0.0032 0.0031 0.0031 0.0027 0.0015 0.0049
Nucleus 0.1339 0.2808 0.249 0.2083 0.1714 0.2801 0.0387 0.1641 0.2615 0.0363 0.1046 0.276 0.1719 0.1991 0.289 0.3991 0.1842 0.3222
Peroxisomes 0.0299 0.0033 0.0025 0.0002 0.0072 0.0004 0.0214 0.0003 0.0008 0.0494 0.0128 0.0197 0.0116 0.0004 0.0076 0.0001 0.0025 0.0012
Punctate Nuclear 0.0524 0.039 0.0521 0.0687 0.0362 0.0533 0.0475 0.0584 0.0469 0.04 0.0354 0.0516 0.0429 0.0617 0.0629 0.064 0.0069 0.0577
Vacuole 0.0054 0.0017 0.0029 0.0007 0.0025 0.0024 0.0072 0.0017 0.0017 0.037 0.0017 0.0018 0.0041 0.0022 0.0024 0.002 0.0043 0.0018
Vacuole Periphery 0.0014 0.0002 0.0014 0.0003 0.0014 0.0007 0.004 0.0002 0.0005 0.0046 0.0008 0.0006 0.0017 0.0004 0.0006 0.0005 0.001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.4164 20.0619 18.195 23.2986 20.2064 13.707 15.2134 21.2967 10.7197 23.1336
Translational Efficiency 1.4632 1.5818 1.8619 0.9216 1.563 2.3916 1.3864 0.5548 1.4157 1.3786

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘