Standard name
Description NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.67 0.90 0.92 0.97 0.83 0.83 0.77 0.62 0.66 0.58 0.62 0.90 0.89 0.93 0.92 0.87 0.84 0.45 0.51 0.55 0.52 0.56 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.06 0 0.06 0 0.22 0.26 0.24 0.47 0.38 0.55 0.45 0 0 0 0 0 0 0.07 0.05 0.14 0.05 0.08 0.05
Nucleus 0.14 0.22 0 0 0 0 0 0.06 0.10 0.09 0.05 0.11 0.09 0.07 0.05 0 0 0.06 0.18 0.19 0.05 0.10 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05
Vac/Vac Membrane 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0.13 0.15 0.09 0.19 0.14 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 7 0 2 2 6 3
Bud 0 0 1 1 0 1 5 5 11 8 4 11 0 0 0 0 0 0 8 4 1 1 6 7
Bud Neck 1 0 11 1 19 9 1 15 3 15 5 13 1 0 0 5 3 0 4 0 1 1 2 5
Bud Site 0 0 0 0 0 0 0 3 8 3 1 5 0 0 0 0 0 0
Cell Periphery 0 0 2 1 0 0 1 3 0 1 0 0 4 1 3 3 10 7 1 0 1 0 1 0
Cytoplasm 275 133 175 184 294 263 343 270 222 213 148 199 141 191 226 270 335 290 175 110 112 98 143 212
Endoplasmic Reticulum 1 2 3 0 1 0 0 0 0 0 0 0 1 0 0 13 31 28 1 1 1 0 5 2
Endosome 0 1 0 0 0 0 1 0 0 0 0 0 0 2 3 1 4 6 9 3 1 1 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 4 2 2 1 0 3 0 0
Mitochondria 101 11 6 11 7 69 106 83 169 122 142 144 2 5 0 3 6 15 26 11 28 9 20 16
Nucleus 55 43 8 8 3 3 15 21 36 28 13 34 14 15 12 13 12 19 70 41 10 18 8 3
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 1 0 0 0 1 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 1 5 8 7 5 4 10 0 0 0 0 3 1 18 6 8 8 9 15
Vac/Vac Membrane 26 21 3 3 1 6 4 7 5 8 4 4 2 3 8 14 27 30 49 31 19 35 36 28
Unique Cell Count 399 198 195 199 303 318 412 351 358 324 257 320 157 215 244 295 384 345 392 218 203 190 255 309
Labelled Cell Count 461 212 212 210 326 352 481 415 463 405 321 420 165 218 253 325 435 400 392 218 203 190 255 309

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.2 4.2 4.1 4.4 3.4 3.2 3.8 3.1 3.2 2.9 3.3 4.6 4.9 4.9 5.7 5.4 5.2 4.7 5.2 5.2
Std Deviation (1e-4) 0.8 1.0 0.9 1.2 1.9 1.2 1.3 1.6 1.2 0.9 1.3 1.2 1.5 1.7 2.1 1.7 1.8 1.9 2.5 3.0 2.0
Intensity Change (Log2) -0.0 0.1 -0.3 -0.4 -0.2 -0.4 -0.4 -0.5 -0.3 0.2 0.2 0.2 0.4 0.4 0.3 0.2 0.3 0.3

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0 0 0