Standard name
Human Ortholog
Description NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.67 0.9 0.92 0.97 0.83 0.83 0.77 0.62 0.66 0.58 0.62 0.9 0.89 0.93 0.92 0.87 0.84 0.45 0.51 0.55 0.52 0.56 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.06 0 0.06 0 0.22 0.26 0.24 0.47 0.38 0.55 0.45 0 0 0 0 0 0 0.07 0.05 0.14 0.05 0.08 0.05
Nucleus 0.14 0.22 0 0 0 0 0 0.06 0.1 0.09 0.05 0.11 0.09 0.07 0.05 0 0 0.06 0.18 0.19 0.05 0.1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05
Vac/Vac Membrane 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0.13 0.15 0.09 0.19 0.14 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 7 0 2 2 6 3
Bud 0 0 1 1 0 1 5 5 11 8 4 11 0 0 0 0 0 0 8 4 1 1 6 7
Bud Neck 1 0 11 1 19 9 1 15 3 15 5 13 1 0 0 5 3 0 4 0 1 1 2 5
Bud Site 0 0 0 0 0 0 0 3 8 3 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 0 0 1 3 0 1 0 0 4 1 3 3 10 7 1 0 1 0 1 0
Cytoplasm 275 133 175 184 294 263 343 270 222 213 148 199 141 191 226 270 335 290 175 110 112 98 143 212
Endoplasmic Reticulum 1 2 3 0 1 0 0 0 0 0 0 0 1 0 0 13 31 28 1 1 1 0 5 2
Endosome 0 1 0 0 0 0 1 0 0 0 0 0 0 2 3 1 4 6 9 3 1 1 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 4 2 2 1 0 3 0 0
Mitochondria 101 11 6 11 7 69 106 83 169 122 142 144 2 5 0 3 6 15 26 11 28 9 20 16
Nucleus 55 43 8 8 3 3 15 21 36 28 13 34 14 15 12 13 12 19 70 41 10 18 8 3
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 1 0 0 0 1 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 2 1 1 1 5 8 7 5 4 10 0 0 0 0 3 1 18 6 8 8 9 15
Vac/Vac Membrane 26 21 3 3 1 6 4 7 5 8 4 4 2 3 8 14 27 30 49 31 19 35 36 28
Unique Cell Count 399 198 195 199 303 318 412 351 358 324 257 320 157 215 244 295 384 345 392 218 203 190 255 309
Labelled Cell Count 461 212 212 210 326 352 481 415 463 405 321 420 165 218 253 325 435 400 392 218 203 190 255 309


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.2 4.2 4.1 4.4 3.4 3.2 3.8 3.1 3.2 2.9 3.3 4.6 4.9 4.9 5.7 5.4 5.2 4.7 5.2 5.2
Std Deviation (1e-4) 0.8 1.0 0.9 1.2 1.9 1.2 1.3 1.6 1.2 0.9 1.3 1.2 1.5 1.7 2.1 1.7 1.8 1.9 2.5 3.0 2.0
Intensity Change (Log2) – – – -0.03 0.08 -0.29 -0.37 -0.15 -0.45 -0.4 -0.5 -0.35 0.16 0.25 0.24 0.45 0.36 0.33 0.17 0.33 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.9 2.8 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1711 -0.208 -0.3577 -0.2803 -0.4882 -0.0433 -0.4676 -1.2423 -1.3195 -1.1833 -1.19 -0.9309 -1.3932 -1.7724 -2.0924 -1.5183 -1.7844 -1.5205
Actin 0.0334 0.0006 0.0063 0.0286 0.0069 0.0051 0.0197 0.0002 0.0032 0.0009 0.004 0.0014 0.0224 0.0007 0.002 0.0269 0.0196 0.0047
Bud 0.0007 0.002 0.0028 0.0026 0.0013 0.0023 0.0032 0.0014 0.0019 0.0009 0.0059 0.0009 0.0006 0.0028 0.0029 0.0017 0.0033 0.0012
Bud Neck 0.0031 0.0005 0.0029 0.0009 0.0012 0.0033 0.0056 0.0007 0.0008 0.0006 0.0015 0.006 0.0108 0.0008 0.0009 0.0046 0.0011 0.0044
Bud Periphery 0.002 0.0019 0.0045 0.0084 0.0014 0.0023 0.0039 0.0015 0.0019 0.002 0.0059 0.0011 0.0008 0.0026 0.0052 0.0025 0.0045 0.0016
Bud Site 0.0015 0.0029 0.0038 0.0018 0.0014 0.0003 0.002 0.0033 0.004 0.0002 0.0018 0.0004 0.0057 0.0073 0.0036 0.002 0.0005 0.0005
Cell Periphery 0.0006 0.0002 0.0004 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0 0.0002 0.0001 0.0003 0.0004 0.0002 0.0002 0.0002 0.0001
Cytoplasm 0.0577 0.1291 0.0654 0.0687 0.0631 0.0975 0.0443 0.0461 0.0441 0.026 0.0567 0.0571 0.0941 0.1249 0.0948 0.0446 0.0795 0.1582
Cytoplasmic Foci 0.0191 0.0034 0.0132 0.0067 0.0055 0.0036 0.0108 0.0035 0.0112 0.0055 0.0057 0.0045 0.0185 0.0072 0.0084 0.0049 0.0245 0.0056
Eisosomes 0.0018 0.0004 0.0008 0.0004 0.0002 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0004 0.0003 0.0001
Endoplasmic Reticulum 0.0033 0.0013 0.0032 0.001 0.0033 0.0012 0.002 0.0007 0.0015 0.0003 0.0013 0.0007 0.0059 0.0038 0.0023 0.0021 0.0018 0.0018
Endosome 0.0097 0.0011 0.0108 0.0017 0.0197 0.0031 0.0167 0.0007 0.0034 0.0046 0.0042 0.0016 0.009 0.0051 0.0041 0.0081 0.0092 0.0033
Golgi 0.0038 0.0002 0.0045 0.0004 0.0043 0.0015 0.0027 0.0001 0.0012 0.0075 0.0017 0.0005 0.0032 0.0001 0.0005 0.0034 0.0025 0.0008
Lipid Particles 0.0099 0.0002 0.0113 0.0004 0.008 0.0008 0.0093 0.0003 0.0047 0.0023 0.0108 0.0033 0.0037 0.0006 0.0023 0.0039 0.0115 0.0004
Mitochondria 0.0052 0.0011 0.006 0.0022 0.0045 0.0015 0.0063 0.0008 0.0026 0.0178 0.0024 0.0025 0.0021 0.0012 0.0025 0.0031 0.0012 0.0011
None 0.421 0.7199 0.7507 0.7637 0.7499 0.4684 0.4023 0.6925 0.7898 0.7983 0.6827 0.4447 0.4165 0.6431 0.7398 0.7255 0.728 0.4583
Nuclear Periphery 0.0109 0.0021 0.0072 0.0026 0.0112 0.0077 0.0157 0.003 0.0055 0.0009 0.0091 0.0068 0.0177 0.007 0.0052 0.0117 0.0018 0.011
Nucleolus 0.0052 0.0026 0.0057 0.0037 0.0028 0.0057 0.0077 0.009 0.0069 0.0039 0.0076 0.0109 0.0047 0.0045 0.0046 0.0051 0.0031 0.0061
Nucleus 0.3792 0.1209 0.0797 0.0928 0.1027 0.3545 0.4015 0.2036 0.0849 0.1211 0.1694 0.4132 0.3516 0.1688 0.1075 0.1318 0.0805 0.3025
Peroxisomes 0.0109 0.0003 0.0055 0.0007 0.0015 0.0006 0.0124 0.0002 0.0083 0.0034 0.004 0.0025 0.0053 0.0002 0.0022 0.0019 0.0203 0.0003
Punctate Nuclear 0.0182 0.0077 0.0106 0.01 0.0084 0.0391 0.0293 0.0299 0.0217 0.0025 0.0227 0.0406 0.0227 0.012 0.0061 0.0131 0.0039 0.0338
Vacuole 0.0019 0.0016 0.0034 0.0021 0.0015 0.0009 0.0025 0.0019 0.0015 0.0006 0.0016 0.0009 0.0033 0.0059 0.0039 0.0017 0.0024 0.0036
Vacuole Periphery 0.0008 0.0002 0.0012 0.0004 0.0011 0.0004 0.0016 0.0002 0.0005 0.0004 0.0007 0.0003 0.0008 0.0008 0.0009 0.0007 0.0005 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.4526 4.4312 10.522 14.8741 2.5707 1.9817 2.6882 4.5359 14.364 9.1182
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3
Localization
Cell Percentages nucleus (37%), cytoplasm (6%), mixed (46%)
Cell Cycle Regulation Yes
nucleus - T/G1 (0.00043)
Subcompartmental Group N/A

Hst4

Hst4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hst4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available