Standard name | |
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Description | Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by human homolog DCAKD and by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | – | 5 | 17 | 6 | 0 | 3 | 4 | 1 | 3 | 1 | 5 | 12 | 6 | 10 | 0 | 5 | 7 | 1 | 0 | 2 | 1 | 5 | 8 |
Bud | 0 | – | 1 | 5 | 0 | 1 | 10 | 6 | 13 | 7 | 16 | 11 | 1 | 2 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 1 | 4 | 4 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 8 | 6 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 3 | 0 | 8 | 6 | 2 | 5 | 0 | 2 | 3 | 1 | 3 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 24 | – | 116 | 179 | 265 | 267 | 269 | 273 | 176 | 163 | 112 | 149 | 274 | 450 | 467 | 30 | 79 | 109 | 13 | 15 | 39 | 66 | 229 | 196 |
Endoplasmic Reticulum | 19 | – | 47 | 49 | 31 | 26 | 17 | 4 | 10 | 22 | 3 | 9 | 109 | 132 | 68 | 3 | 6 | 9 | 68 | 21 | 121 | 32 | 123 | 201 |
Endosome | 0 | – | 6 | 11 | 10 | 31 | 47 | 61 | 49 | 60 | 45 | 79 | 35 | 35 | 72 | 15 | 34 | 43 | 1 | 1 | 9 | 4 | 11 | 23 |
Golgi | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 1 | 5 |
Mitochondria | 0 | – | 1 | 9 | 3 | 26 | 48 | 30 | 184 | 142 | 162 | 194 | 1 | 3 | 2 | 3 | 5 | 8 | 10 | 4 | 42 | 13 | 37 | 34 |
Nucleus | 2 | – | 1 | 3 | 4 | 5 | 5 | 8 | 10 | 7 | 12 | 12 | 0 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 2 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 3 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 2 | 4 | 0 | 5 | 8 | 5 | 7 | 3 | 4 | 4 | 0 | 3 | 2 | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 7 | – | 29 | 21 | 35 | 96 | 161 | 186 | 139 | 161 | 113 | 168 | 19 | 25 | 44 | 13 | 21 | 28 | 10 | 3 | 11 | 12 | 40 | 50 |
Unique Cell Count | 51 | 191 | 269 | 338 | 422 | 513 | 522 | 490 | 493 | 385 | 538 | 425 | 612 | 628 | 58 | 143 | 195 | 115 | 48 | 240 | 140 | 474 | 550 | |
Labelled Cell Count | 54 | 210 | 296 | 367 | 458 | 570 | 587 | 590 | 580 | 474 | 640 | 460 | 657 | 675 | 67 | 157 | 217 | 115 | 48 | 240 | 140 | 474 | 550 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 6.6 | – | 7.2 | 6.9 | 7.4 | 6.3 | 6.2 | 6.7 | 5.6 | 5.9 | 5.9 | 6.0 | 7.1 | 7.3 | 7.1 | 6.8 | 8.3 | 8.7 | 8.0 | 7.9 | 8.3 |
Std Deviation (1e-4) | 0.9 | – | 1.4 | 1.4 | 1.5 | 1.3 | 1.5 | 2.2 | 1.1 | 1.1 | 3.4 | 1.0 | 1.0 | 0.9 | 0.9 | 1.3 | 1.6 | 1.9 | 1.5 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.02 | -0.21 | -0.22 | -0.12 | -0.38 | -0.3 | -0.29 | -0.28 | -0.02 | 0.01 | -0.02 | -0.09 | 0.19 | 0.26 | 0.14 | 0.12 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 4.3 | 0.6 | -2.0 | -2.0 | -5.9 | -6.6 | -7.3 | -8.2 | 0.9 | 3.4 | 3.6 | -1.2 | -1.0 | -1.0 |
Endoplasmic Reticulum | -1.7 | -4.8 | -6.5 | -8.7 | -10.9 | -9.6 | -7.8 | -9.6 | -10.2 | 0.3 | -0.9 | -4.8 | -3.2 | -5.1 | -5.6 |
Endosome | 0.5 | -0.1 | 2.0 | 2.7 | 3.5 | 3.0 | 3.6 | 3.4 | 4.3 | 2.3 | 1.4 | 3.4 | 0 | 5.7 | 5.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.2 | 4.1 | 3.0 | 9.8 | 8.2 | 10.4 | 9.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.5 | -1.6 | 2.2 | 4.3 | 5.3 | 3.6 | 4.6 | 3.7 | 4.3 | -4.6 | -5.3 | -3.5 | 1.3 | -0.1 | -0.2 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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