Standard name
Human Ortholog
Description Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by human homolog DCAKD and by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.61 0.67 0.78 0.63 0.52 0.52 0.36 0.33 0.29 0.28 0.64 0.74 0.74 0.52 0.55 0.56 0.11 0.32 0.16 0.48 0.48 0.36
Endoplasmic Reticulum 0.37 0.25 0.18 0.09 0.06 0 0 0 0 0 0 0.26 0.22 0.11 0.05 0 0.05 0.6 0.44 0.51 0.23 0.26 0.37
Endosome 0 0 0 0 0.07 0.09 0.12 0.1 0.12 0.12 0.15 0.08 0.06 0.11 0.26 0.24 0.22 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.09 0.06 0.38 0.29 0.42 0.36 0 0 0 0.05 0 0 0.09 0.09 0.18 0.09 0.08 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.15 0.08 0.1 0.23 0.31 0.36 0.28 0.33 0.29 0.31 0 0 0.07 0.22 0.15 0.14 0.09 0.08 0.05 0.09 0.09 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 5 17 6 0 3 4 1 3 1 5 12 6 10 0 5 7 1 0 2 1 5 8
Bud 0 1 5 0 1 10 6 13 7 16 11 1 2 1 0 2 3 2 0 0 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 8 6
Bud Site 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0 0
Cell Periphery 0 3 0 8 6 2 5 0 2 3 1 3 3 3 1 2 0 0 0 0 0 1 2
Cytoplasm 24 116 179 265 267 269 273 176 163 112 149 274 450 467 30 79 109 13 15 39 66 229 196
Endoplasmic Reticulum 19 47 49 31 26 17 4 10 22 3 9 109 132 68 3 6 9 68 21 121 32 123 201
Endosome 0 6 11 10 31 47 61 49 60 45 79 35 35 72 15 34 43 1 1 9 4 11 23
Golgi 1 1 0 1 0 0 0 0 1 0 0 2 0 2 0 0 3 2 0 1 0 1 5
Mitochondria 0 1 9 3 26 48 30 184 142 162 194 1 3 2 3 5 8 10 4 42 13 37 34
Nucleus 2 1 3 4 5 5 8 10 7 12 12 0 1 3 0 1 2 0 0 0 0 2 2
Nuclear Periphery 0 0 0 0 0 1 0 0 2 0 5 0 0 0 0 0 0 2 0 1 0 2 3
Nucleolus 0 0 0 0 0 1 1 2 3 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 4 0 5 8 5 7 3 4 4 0 3 2 2 5 0 0 0 0 1 3
Vac/Vac Membrane 7 29 21 35 96 161 186 139 161 113 168 19 25 44 13 21 28 10 3 11 12 40 50
Unique Cell Count 51 191 269 338 422 513 522 490 493 385 538 425 612 628 58 143 195 115 48 240 140 474 550
Labelled Cell Count 54 210 296 367 458 570 587 590 580 474 640 460 657 675 67 157 217 115 48 240 140 474 550


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.2 6.9 7.4 6.3 6.2 6.7 5.6 5.9 5.9 6.0 7.1 7.3 7.1 6.8 8.3 8.7 8.0 7.9 8.3
Std Deviation (1e-4) 0.9 1.4 1.4 1.5 1.3 1.5 2.2 1.1 1.1 3.4 1.0 1.0 0.9 0.9 1.3 1.6 1.9 1.5 1.3 1.5
Intensity Change (Log2) -0.07 0.02 -0.21 -0.22 -0.12 -0.38 -0.3 -0.29 -0.28 -0.02 0.01 -0.02 -0.09 0.19 0.26 0.14 0.12 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0.6
Bud 0 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 4.3 0.6 -2.0 -2.0 -5.9 -6.6 -7.3 -8.2 0.9 3.4 3.6 -1.2 -1.0 -1.0
Endoplasmic Reticulum -1.7 -4.8 -6.5 -8.7 -10.9 -9.6 -7.8 -9.6 -10.2 0.3 -0.9 -4.8 -3.2 -5.1 -5.6
Endosome 0.5 -0.1 2.0 2.7 3.5 3.0 3.6 3.4 4.3 2.3 1.4 3.4 0 5.7 5.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.2 4.1 3.0 9.8 8.2 10.4 9.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.5 -1.6 2.2 4.3 5.3 3.6 4.6 3.7 4.3 -4.6 -5.3 -3.5 1.3 -0.1 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7714 1.9453 1.7186 1.8705 1.4669 1.7965 3.4316 4.1744 3.4893 3.6711 3.008 3.5869 1.9865 2.6595 2.1133 2.0493 2.3437 2.1536
Actin 0.0043 0.0052 0.0039 0.0037 0.002 0.0013 0.0198 0.0004 0.0172 0.0054 0.0042 0.004 0.0235 0.0016 0.0043 0.0358 0.0008 0.001
Bud 0.0011 0.0005 0.0047 0.0071 0.0233 0.0012 0.0002 0 0.0005 0.0001 0.0001 0.0001 0.0002 0.0009 0.0003 0.0005 0.0004 0.0001
Bud Neck 0.0003 0.0007 0.0005 0.0009 0.0005 0.0011 0.0013 0.0006 0.0019 0.0086 0.0004 0.0016 0.0006 0.0004 0.0005 0.0006 0.0156 0.0009
Bud Periphery 0.0006 0.0003 0.001 0.0041 0.0061 0.0008 0.0003 0.0001 0.0008 0.0001 0.0001 0.0002 0.0003 0.0003 0.0002 0.0005 0.0002 0.0001
Bud Site 0.0023 0.016 0.0021 0.0093 0.0043 0.0002 0.0038 0.0019 0.0032 0.0011 0.0001 0.0007 0.0008 0.0095 0.0017 0.0022 0.0012 0.0001
Cell Periphery 0.0003 0.0007 0.0007 0.0011 0.0004 0.0003 0.0016 0.001 0.0013 0.0006 0.0004 0.0007 0.0009 0.0008 0.0009 0.0005 0.0003 0.0002
Cytoplasm 0.1177 0.1904 0.184 0.2168 0.1602 0.1816 0.1597 0.1986 0.2929 0.2753 0.2639 0.2728 0.1528 0.3309 0.2736 0.1879 0.2793 0.2822
Cytoplasmic Foci 0.0549 0.0577 0.0683 0.0657 0.0481 0.0638 0.0597 0.0846 0.0674 0.0446 0.0703 0.0415 0.0578 0.0505 0.0608 0.0436 0.0559 0.0474
Eisosomes 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0 0.0003 0.0018 0.0001 0.0004 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.2858 0.2485 0.282 0.2939 0.2364 0.3181 0.2958 0.3135 0.2576 0.2531 0.3472 0.3814 0.334 0.3337 0.2938 0.3759 0.2874 0.3754
Endosome 0.2873 0.2627 0.2817 0.2181 0.3084 0.2602 0.1634 0.2189 0.1452 0.1373 0.1564 0.1165 0.1715 0.1398 0.1921 0.1814 0.1943 0.1438
Golgi 0.0149 0.0222 0.028 0.0129 0.0172 0.0129 0.0239 0.0092 0.0145 0.0178 0.0156 0.0081 0.0155 0.0105 0.0122 0.0307 0.0143 0.0137
Lipid Particles 0.112 0.0799 0.0455 0.0138 0.0413 0.0464 0.0896 0.0974 0.0729 0.04 0.0581 0.0629 0.1155 0.0528 0.0732 0.0589 0.019 0.0698
Mitochondria 0.0062 0.0055 0.0058 0.01 0.006 0.0065 0.0128 0.0007 0.013 0.0055 0.0074 0.0074 0.0021 0.0009 0.0015 0.0048 0.002 0.0053
None 0.0174 0.0207 0.0241 0.04 0.0491 0.0221 0.0932 0.0417 0.056 0.1202 0.0485 0.0627 0.0325 0.0119 0.0349 0.0228 0.0664 0.0223
Nuclear Periphery 0.0571 0.0507 0.0363 0.0243 0.0426 0.0453 0.0284 0.0121 0.0137 0.023 0.0146 0.0124 0.042 0.0252 0.021 0.0165 0.0285 0.0186
Nucleolus 0.0005 0.0003 0.0003 0.0043 0.0019 0.0007 0.0008 0.0001 0.0016 0.0036 0.0001 0.0009 0.0008 0.0005 0.0003 0.0004 0.0003 0.0001
Nucleus 0.005 0.0071 0.0042 0.0123 0.0133 0.0061 0.0039 0.0011 0.0037 0.0337 0.0015 0.0036 0.005 0.0032 0.0025 0.0028 0.0028 0.0031
Peroxisomes 0.003 0.0018 0.0008 0.0008 0.0008 0.001 0.0132 0.0009 0.0106 0.0036 0.0018 0.0013 0.0034 0.0005 0.0014 0.0019 0.0019 0.0007
Punctate Nuclear 0.0051 0.0034 0.0052 0.0403 0.0063 0.0098 0.0057 0.0018 0.0092 0.0121 0.003 0.0039 0.0098 0.0037 0.0053 0.0153 0.0038 0.0038
Vacuole 0.0188 0.0202 0.0168 0.0158 0.0244 0.0148 0.0196 0.0137 0.0143 0.0103 0.0048 0.0147 0.0278 0.0204 0.0172 0.0134 0.0222 0.0091
Vacuole Periphery 0.0052 0.0058 0.0042 0.0046 0.0077 0.0057 0.003 0.0017 0.0022 0.0023 0.0016 0.0021 0.0031 0.0019 0.0022 0.0033 0.0035 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.2778 31.2488 28.2288 26.7627 25.617 37.975 22.6665 28.2787 31.4584 27.9576
Translational Efficiency 1.3048 0.8238 0.7206 0.9308 1.0337 0.669 1.3622 0.9657 0.9336 1.0762

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by human homolog DCAKD and by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets
Localization
Cell Percentages ER (71%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Cab5

Cab5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cab5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available