Standard name
Description Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.84 0.75 0.87 0.83 0.83 0.69 0.65 0.68 0.71 0.68 0.67 0.88 0.79 0.71 0.81 0.78 0.22 0.35 0.25 0.19 0.14 0.15
Endoplasmic Reticulum 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.14 0 0 0 0 0.05 0.05 0.06
Endosome 0.1 0 0.09 0.05 0 0 0 0 0 0 0 0 0 0.08 0.12 0.1 0 0.23 0.16 0.23 0.11 0.16 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.17 0.26 0.32 0.49 0.43 0.53 0.38 0.49 0.55 0 0 0 0 0 0.07 0.05 0.15 0.14 0.11 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.08 0.06 0 0.06 0 0.05 0 0 0.06 0 0 0.05 0.17 0.24 0.14 0.22 0.38 0.36 0.28 0.45 0.49 0.49
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 0 0 0 0 0 1 3 2 3 0 1 4 4 0 0 1 2 4 1 2 2
Bud 0 1 3 5 11 17 24 34 33 26 35 35 2 1 2 0 0 2 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 1 0 1 1 1
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 0 1 0 0 2 0 1 0 6 7 6 12 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 173 169 192 303 408 513 562 555 469 618 435 576 218 376 367 17 14 58 77 76 45 49 56
Endoplasmic Reticulum 15 13 6 1 1 2 4 9 1 7 8 8 15 13 3 3 0 8 4 10 11 16 21
Endosome 23 5 22 19 13 6 11 19 9 18 5 4 4 36 60 2 0 61 36 70 25 55 46
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 7 2 2 5 6 7
Mitochondria 5 0 41 60 126 195 401 371 362 328 313 477 0 1 0 0 0 20 10 48 34 39 39
Nucleus 1 0 1 0 0 7 7 16 4 3 3 0 4 2 1 0 0 0 1 0 1 0 1
Nuclear Periphery 1 0 0 0 1 1 10 3 1 1 1 1 0 0 0 0 0 1 0 3 0 1 1
Nucleolus 0 0 0 0 0 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 1 1 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 2 2 1 3 2 0 7 2 2 0 2 5 0 0 2 0 3 0 2 1
Vac/Vac Membrane 36 16 16 8 31 23 37 35 29 50 25 23 12 82 126 3 4 102 80 86 107 172 181
Unique Cell Count 237 201 257 350 494 619 813 855 689 867 643 865 248 477 519 21 18 270 223 313 240 351 368
Labelled Cell Count 257 209 284 399 596 766 1061 1050 919 1068 836 1138 258 517 570 25 18 270 223 313 240 351 368


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.7 4.7 4.6 4.3 4.5 4.1 4.4 4.1 4.4 4.1 4.2 6.5 6.8 7.0 8.3 8.1 7.4 7.2 7.6
Std Deviation (1e-4) 0.9 1.0 0.8 1.2 1.2 1.2 1.2 1.2 1.0 1.2 1.1 0.9 0.8 1.1 1.0 1.6 1.4 1.6 1.2 1.4
Intensity Change (Log2) -0.02 -0.12 -0.05 -0.19 -0.07 -0.19 -0.07 -0.17 -0.15 0.47 0.55 0.6 0.83 0.79 0.67 0.63 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.7 2.6 2.8 -1.7 -2.9 -2.0 -1.1 -2.1 -2.5 3.8 1.3 -1.2 0.6 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.1 0.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 -3.5 -0.5 1.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.6 -8.8 -9.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.5 0 -1.6 -1.1 -1.4 -1.3 -0.3 -1.5 -2.7 -0.7 4.2 6.1 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rav2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available