Standard name
Description Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.06 0.05 0.05 0 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0 0.07 0.05 0.08 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.1 0.08 0.05 – 0.05 0 0 0 0 0.06 0.06 0.06 0.07 0.07 0.11 0 0 0 0 0 0
Cytoplasm 0.3 0.44 0.3 0.3 0.48 0.36 – 0.27 0.23 0.21 0.19 0.17 0.44 0.66 0.66 0.81 0.8 0.62 0.05 0.16 0 0.1 0.08 0.09
Endoplasmic Reticulum 0.62 0.51 0.54 0.39 0.06 0.15 – 0.1 0.08 0.11 0.11 0.13 0.54 0.34 0.31 0 0.07 0 0.7 0.57 0.7 0.56 0.63 0.68
Endosome 0 0 0.06 0.07 0.17 0.07 – 0.12 0.06 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0
Golgi 0 0 0.05 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0.05 0 0
Mitochondria 0 0.06 0.09 0.26 0.16 0.46 – 0.53 0.67 0.69 0.77 0.77 0 0 0 0.11 0.06 0.09 0 0.06 0.05 0.14 0.08 0.05
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.16 0.06 – 0.05 0.08 0.06 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 19 13 9 2 5 – 10 8 8 3 9 4 1 5 11 7 7 17 15 21 7 26 15
Bud 1 3 3 4 6 5 – 14 18 18 19 16 1 0 0 1 2 0 1 3 0 1 3 2
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 4 0 2 7 0 0 4 3
Bud Site 0 2 0 0 0 0 – 2 2 3 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 9 11 18 22 16 – 22 16 14 10 15 6 8 11 17 9 15 1 6 3 1 8 8
Cytoplasm 71 139 71 55 134 122 – 116 99 104 78 73 41 85 122 183 102 83 13 61 7 14 27 32
Endoplasmic Reticulum 145 161 130 71 18 49 – 44 35 55 45 54 51 43 57 9 9 6 193 216 214 81 219 240
Endosome 5 2 14 12 48 25 – 50 26 22 12 10 1 0 1 6 3 7 9 13 10 6 8 4
Golgi 7 5 11 9 1 1 – 0 2 0 1 2 0 0 1 0 0 2 18 16 21 7 9 8
Mitochondria 4 20 21 48 45 154 – 226 292 336 310 329 1 0 0 25 7 12 11 21 16 19 28 18
Nucleus 0 0 1 1 1 1 – 2 0 1 1 0 0 1 1 2 4 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 3 1 0 – 4 2 1 2 4 0 0 0 0 1 0 0 1 1 0 0 0
Nucleolus 0 0 1 0 0 0 – 1 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 3 6 2 – 3 2 4 3 5 0 0 0 0 0 0 1 0 2 0 0 1
SpindlePole 1 0 1 1 2 2 – 7 6 11 3 2 0 0 0 5 1 4 0 1 0 0 0 0
Vac/Vac Membrane 13 4 9 5 45 21 – 22 36 29 16 13 1 2 1 2 3 9 2 11 2 3 7 12
Unique Cell Count 233 317 240 182 281 336 424 438 488 402 428 94 128 184 227 127 134 275 381 306 146 351 352
Labelled Cell Count 271 364 287 239 331 403 523 546 606 505 534 106 140 199 261 152 146 275 381 306 146 351 352


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 7.0 7.2 6.9 6.9 5.6 – 5.6 5.1 5.0 4.9 4.8 7.3 7.5 7.6 5.6 6.4 8.0 8.2 8.3 8.7
Std Deviation (1e-4) 0.8 1.0 1.7 1.5 1.8 1.1 – 1.0 1.1 1.1 1.0 1.0 1.0 0.9 1.0 0.8 1.2 1.3 1.4 1.4 1.8
Intensity Change (Log2) – – – -0.05 -0.06 -0.37 – -0.37 -0.5 -0.53 -0.54 -0.58 0.02 0.07 0.09 -0.35 -0.17 0.15 0.19 0.21 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -3.2 -2.7 – -2.1 -2.6 -2.9 -3.7 0 0 0 -1.4 -0.3 0 -0.1
Bud 0 0 0 – 1.6 2.1 1.8 2.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.1 1.5 0.1 – 0.3 -0.6 -1.2 -1.4 0 0 0.7 0.6 1.3 1.0 2.4
Cytoplasm 0.1 4.2 1.7 – -0.6 -2.0 -2.5 -3.0 -3.8 2.4 6.8 7.5 11.1 9.3 6.1
Endoplasmic Reticulum -3.1 -12.0 -10.1 – -12.3 -13.4 -12.5 -11.8 0 0 -3.8 -4.8 -11.9 -8.8 -9.6
Endosome 0.3 4.0 0.8 – 2.5 0.1 -0.8 -1.8 0 0 0 -2.9 -1.7 -1.5 -0.2
Golgi 0.2 -3.2 -3.6 – 0 0 0 0 0 0 0 -2.5 -3.3 0 0
Mitochondria 4.8 2.5 9.5 – 11.4 14.5 15.2 16.8 0 -2.5 -3.4 -4.1 0.8 -1.1 0.1
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 4.6 1.3 – 0.8 2.2 1.3 0.1 -0.5 0 0 0 -2.0 0 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7526 4.8294 4.1124 3.8599 3.7407 4.3328 4.4575 5.7703 5.3874 5.1803 5.3028 5.3909 6.0231 7.5479 6.8879 6.5556 6.6073 6.8747
Actin 0.0752 0.0088 0.0605 0.0142 0.0038 0.0056 0.0329 0.009 0.0155 0.02 0.0269 0.0201 0.0464 0.0181 0.0061 0.0064 0.0061 0.006
Bud 0.0008 0.0013 0.0009 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0003 0.0002 0.0003 0.0004 0.0002 0.0001 0.0002 0.0036 0.0001
Bud Neck 0.0063 0.0008 0.0012 0.0012 0.0004 0.002 0.0027 0.0008 0.0005 0.0011 0.0009 0.0091 0.0022 0.0009 0.0019 0.0009 0.0013 0.0028
Bud Periphery 0.0012 0.0009 0.0014 0.0001 0.0001 0.0001 0.0005 0.0002 0.0002 0.0004 0.0003 0.0005 0.0005 0.0002 0.0002 0.0003 0.0027 0.0001
Bud Site 0.0069 0.0147 0.0124 0.0004 0.0001 0.0001 0.0039 0.0088 0.0015 0.0031 0.0006 0.0005 0.0063 0.0051 0.005 0.0006 0.0006 0.0002
Cell Periphery 0.0013 0.0031 0.0018 0.0004 0.0003 0.0006 0.0024 0.0041 0.003 0.002 0.0019 0.0023 0.0027 0.0042 0.0022 0.003 0.0009 0.0007
Cytoplasm 0.2585 0.2653 0.2409 0.3191 0.2454 0.229 0.1907 0.3564 0.2875 0.2639 0.4362 0.2414 0.1992 0.2194 0.2335 0.1687 0.1756 0.1446
Cytoplasmic Foci 0.0307 0.0294 0.0318 0.0432 0.0202 0.0398 0.0555 0.0586 0.0271 0.0467 0.0756 0.0862 0.054 0.0652 0.0373 0.0269 0.0918 0.0727
Eisosomes 0.0008 0.0001 0.0007 0 0 0 0.0005 0.0002 0.0001 0.0003 0.0002 0.0002 0.0008 0.0001 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.3922 0.5366 0.4909 0.5236 0.5883 0.4978 0.4286 0.3325 0.5071 0.4801 0.2225 0.3609 0.4367 0.4673 0.5751 0.5833 0.3972 0.4252
Endosome 0.0654 0.0658 0.0533 0.0319 0.0936 0.1222 0.0932 0.1018 0.0624 0.0822 0.0581 0.1357 0.1077 0.0917 0.054 0.0687 0.1685 0.163
Golgi 0.0271 0.0305 0.0402 0.0131 0.0169 0.0578 0.0428 0.0452 0.0255 0.0388 0.0268 0.0733 0.0529 0.079 0.0377 0.0385 0.075 0.1257
Lipid Particles 0.0356 0.0137 0.0156 0.01 0.0118 0.0194 0.0513 0.009 0.0129 0.0174 0.0343 0.0164 0.0474 0.0258 0.0155 0.0633 0.0372 0.0294
Mitochondria 0.0135 0.0023 0.0177 0.0005 0.0015 0.0089 0.0218 0.0009 0.0016 0.0028 0.0022 0.0081 0.004 0.0029 0.0036 0.0022 0.0042 0.008
None 0.0153 0.0087 0.0061 0.0053 0.0013 0.004 0.0221 0.062 0.0431 0.0199 0.0644 0.0201 0.0082 0.0025 0.0128 0.0124 0.0164 0.0053
Nuclear Periphery 0.0207 0.0069 0.0085 0.0296 0.0095 0.0057 0.0107 0.0033 0.0052 0.0076 0.0279 0.0034 0.0127 0.0053 0.0074 0.0057 0.0046 0.0029
Nucleolus 0.0005 0.0001 0.0002 0 0 0 0.0004 0.0001 0.0001 0.0002 0.0003 0.0001 0.0003 0.0001 0 0.0001 0.0003 0.0001
Nucleus 0.0064 0.0012 0.0011 0.0026 0.0022 0.001 0.0027 0.0008 0.001 0.0014 0.0031 0.0008 0.002 0.0008 0.0009 0.0007 0.0016 0.0005
Peroxisomes 0.0275 0.005 0.0082 0.0013 0.0003 0.0008 0.0171 0.0006 0.001 0.0014 0.0059 0.0161 0.0071 0.0062 0.0025 0.0124 0.0026 0.0027
Punctate Nuclear 0.0047 0.001 0.0027 0.001 0.0004 0.0005 0.0136 0.0007 0.0007 0.0051 0.009 0.0007 0.0039 0.001 0.0005 0.0006 0.0019 0.0006
Vacuole 0.0069 0.0032 0.0029 0.0017 0.0028 0.0031 0.0045 0.004 0.0034 0.0045 0.0016 0.0029 0.0035 0.0033 0.003 0.0042 0.0064 0.0058
Vacuole Periphery 0.0025 0.0006 0.001 0.0006 0.0009 0.0015 0.0019 0.0005 0.0005 0.0008 0.001 0.0009 0.0011 0.0007 0.0005 0.0008 0.0014 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.6828 9.0701 11.7782 16.5902 11.5107 13.6899 16.966 16.7121 15.1047 17.5853
Translational Efficiency 0.7268 0.9076 0.7262 0.4024 0.4786 0.7207 0.5616 0.5043 0.578 0.5338

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘