Standard name
Description Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, represses transcription by recruiting conserved histone deacetylase Rpd3p (through co-repressor Sin3p) and chromatin-remodeling factor Isw2p; couples metabolic responses to nutritional cues with initiation and progression of meiosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.06 0.08 0.05 0.07 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.05 0.07 0.05 0.06 0.05 0.06 0.07 0.09 0.15 0.09 0.08 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.06 0.05 0.06 0 0.18 0.26 0.47 0.57 0.55 0.42 0.07 0 0 0.07 0.05 0.1 0 0 0 0 0 0
Nucleus 0.92 0.92 0.93 0.93 0.91 0.85 0.82 0.78 0.82 0.71 0.78 0.92 0.89 0.82 0.85 0.85 0.84 0.87 0.86 0.83 0.74 0.73 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0.08 0.12 0.15 0.13 0 0 0 0 0 0 0.05 0 0 0.07 0.09 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.13 0.11 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 1
Bud 0 0 0 0 0 0 2 3 10 4 4 0 0 0 0 1 1 3 2 1 4 4 10
Bud Neck 0 0 1 0 0 0 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0 2 4
Bud Site 0 1 1 1 6 7 18 12 17 10 11 2 0 0 0 0 2
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 7 10 12 16 18 25 10 12 9 9 29 40 82 33 29 26 0 0 0 1 0 1
Endoplasmic Reticulum 1 2 0 0 0 0 1 0 0 0 0 0 0 0 7 7 11 0 0 0 0 2 3
Endosome 0 3 1 1 0 0 0 1 0 0 0 1 1 1 5 2 3 4 4 0 2 4 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 0 0 2 1
Mitochondria 19 22 15 15 10 71 94 91 122 107 68 29 12 12 25 18 30 2 1 2 2 2 5
Nucleus 348 343 308 249 244 327 302 153 174 138 124 402 409 448 320 322 242 338 340 284 274 314 287
Nuclear Periphery 0 0 2 0 2 2 1 2 1 2 0 3 5 4 1 4 5 1 0 0 0 1 1
Nucleolus 29 15 5 6 3 11 16 15 25 30 21 10 16 19 6 10 8 20 10 12 26 38 48
Peroxisomes 1 1 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0 0 1 0 0
SpindlePole 1 1 7 4 4 4 13 0 1 4 2 1 6 4 0 2 4 0 3 4 0 6 5
Vac/Vac Membrane 4 6 4 2 3 2 2 1 4 2 0 1 3 8 6 13 11 12 24 29 46 46 56
Unique Cell Count 377 372 330 268 268 386 367 195 213 194 160 437 459 547 377 379 288 387 396 344 370 433 438
Labelled Cell Count 410 401 354 290 288 443 476 288 368 308 239 480 493 579 403 408 344 387 396 344 370 433 438


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.4 6.1 5.7 6.1 4.9 5.0 4.8 4.3 4.3 4.7 5.7 5.7 5.5 8.4 8.9 7.9 5.4 5.4 5.6
Std Deviation (1e-4) 0.8 1.2 1.2 1.4 1.4 1.3 1.3 1.6 1.4 1.4 1.7 1.2 1.2 1.4 2.2 2.8 2.2 1.2 1.3 1.4
Intensity Change (Log2) -0.09 0.01 -0.3 -0.28 -0.34 -0.49 -0.5 -0.38 -0.1 -0.1 -0.13 0.46 0.56 0.37 -0.16 -0.16 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.7 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.8 1.1 2.3 1.2 1.5 1.0 1.4 2.3 3.2 5.6 3.2 2.7 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 -0.5 5.7 7.6 0 0 0 0 1.2 -1.5 -2.0 1.2 0.1 2.8
Nucleus -0.2 -1.0 -3.6 -4.4 -5.0 -4.2 -6.9 -5.1 -0.7 -2.0 -4.8 -3.6 -3.5 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.2 2.2 0 0 0 0 0.8 1.7 1.7 0.1 1.0 1.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 -1.2 1.1 0 0 0 0 0 -0.9 0 0 0 -0.7
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.9 2.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ume6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available