Standard name
Human Ortholog
Description Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.96 0.94 0.92 0.87 0.79 0.78 0.71 0.7 0.65 1.0 0.99 0.98 0.96 0.95 0.98 0.98 0.98 0.9 1.0 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.06 0.06 0.09 0.11 0.17 0.17 0.21 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 8 11 6 3 7 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 0 5 0 1 3 0 0 0 0 1 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 3 2 2 12 9 8 6 1 6 2 4 3 13 14 1 0 0 0 0 0 0
Cytoplasm 177 177 165 171 209 231 311 213 221 179 129 135 178 132 192 245 301 214 175 165 158 117 217 187
Endoplasmic Reticulum 0 0 1 2 1 6 17 17 10 16 7 5 0 0 2 6 13 7 0 0 0 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 2 2 8 6 1 10 0 1 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 4 1 9 5 9 3 0 0 0 1 0 0 0 0 1 0 0 0
Nucleus 0 0 1 0 0 2 9 8 4 4 5 7 0 0 0 1 0 1 0 0 3 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 2 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 4 17 14 20 23 30 42 31 43 2 0 0 7 11 6 0 1 1 0 2 1
Unique Cell Count 177 178 167 178 222 252 359 269 285 253 183 209 178 133 196 255 316 219 179 169 175 118 225 201
Labelled Cell Count 177 181 171 180 229 255 380 281 307 265 188 221 182 137 199 273 341 231 179 169 175 118 225 201


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.0 34.6 27.7 25.2 27.6 26.2 27.8 27.3 26.6 27.1 25.4 26.9 28.2 32.5 35.4 56.6 59.4 55.9 28.3 32.5 38.4
Std Deviation (1e-4) 5.0 6.0 6.8 6.2 9.0 6.4 7.5 7.7 8.8 8.7 8.8 9.1 6.3 7.5 9.5 23.6 23.4 22.4 6.9 8.7 10.5
Intensity Change (Log2) – – – -0.14 -0.0 -0.08 0.01 -0.02 -0.05 -0.03 -0.12 -0.04 0.03 0.23 0.36 1.03 1.1 1.01 0.03 0.23 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.7 1.5 0
Cytoplasm -1.6 -2.4 -3.1 -4.4 -5.9 -6.2 -7.3 -7.2 -8.2 1.5 0.4 -0.6 -1.6 -2.0 -0.8
Endoplasmic Reticulum 0 0 0 2.4 2.9 0 2.9 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.3 2.7 2.7 3.5 4.0 5.3 5.3 6.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.7675 24.7644 22.5787 21.7453 19.092 23.694 20.8082 25.2474 23.0089 19.9359 22.5717 22.0938 13.7404 21.8995 19.1984 17.7166 19.854 18.1075
Actin 0.0175 0.0002 0.0007 0.0006 0.0004 0.0001 0.0026 0.0003 0.0001 0.0002 0.0004 0.0001 0.0156 0.0002 0.0023 0.0128 0.0002 0.007
Bud 0.0008 0.0001 0.0004 0.0008 0.0011 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0017 0 0.0005 0.0023 0 0.0001
Bud Neck 0.0006 0.0001 0.0003 0.0005 0.0009 0.0004 0.0004 0.0002 0.0003 0.0004 0.0005 0.0007 0.0009 0.0001 0.0003 0.0008 0.0002 0.0006
Bud Periphery 0.0005 0 0.0002 0.0003 0.0005 0 0 0 0 0 0 0 0.0019 0 0.0003 0.0034 0 0.0001
Bud Site 0.0019 0.0001 0.0007 0.0024 0.001 0 0.0001 0.0001 0 0.0001 0 0 0.0022 0 0.0006 0.0218 0 0.0001
Cell Periphery 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0 0 0 0 0.0002 0 0.0001 0.0006 0 0
Cytoplasm 0.8628 0.9931 0.9495 0.8978 0.8849 0.974 0.9598 0.9816 0.9729 0.9455 0.979 0.9596 0.7511 0.9906 0.9269 0.907 0.8704 0.932
Cytoplasmic Foci 0.0179 0.0005 0.0055 0.0074 0.0132 0.0005 0.0006 0.0004 0.0004 0.0011 0.0005 0.0006 0.0199 0.0002 0.0089 0.0043 0.0004 0.0088
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0084 0.0003 0.0016 0.0012 0.0033 0.0007 0.0021 0.0007 0.0008 0.0013 0.0012 0.0017 0.0047 0.0002 0.001 0.0028 0.0002 0.0034
Endosome 0.0232 0.0001 0.0124 0.025 0.0593 0.0002 0.0004 0.0001 0.0001 0.0005 0.0002 0.0004 0.0291 0 0.0094 0.0052 0.0003 0.009
Golgi 0.0084 0 0.0036 0.0038 0.0026 0 0.0001 0 0 0 0 0 0.0109 0 0.0048 0.0022 0.0003 0.0066
Lipid Particles 0.0036 0 0.0004 0.0007 0.0003 0 0 0 0 0.0001 0 0 0.0134 0 0.0051 0.0064 0.0001 0.0019
Mitochondria 0.0036 0 0.0037 0.0292 0.0009 0.0001 0.0001 0 0 0 0 0.0001 0.0231 0 0.0145 0.0046 0.1237 0.001
None 0.0047 0.0003 0.0006 0.0006 0.0042 0.0002 0.0007 0.0002 0.0003 0.0005 0.0003 0.0003 0.0096 0.0002 0.002 0.0035 0.0001 0.0006
Nuclear Periphery 0.0099 0.0006 0.0018 0.003 0.0026 0.002 0.0044 0.0016 0.0025 0.0073 0.0023 0.0037 0.0203 0.001 0.0034 0.0026 0.0004 0.0039
Nucleolus 0.0008 0 0.0001 0.0001 0.0002 0 0 0 0 0.0001 0 0 0.001 0 0.0003 0.0014 0 0
Nucleus 0.0255 0.0042 0.0135 0.0099 0.0105 0.0212 0.0273 0.0143 0.0217 0.0408 0.0145 0.0317 0.0582 0.0072 0.0117 0.0099 0.0028 0.0212
Peroxisomes 0.0015 0 0.0004 0.0007 0.0002 0 0 0 0 0 0 0 0.0184 0 0.0025 0.0026 0.0002 0.0019
Punctate Nuclear 0.0032 0.0002 0.0004 0.0003 0.0005 0.0002 0.0003 0.0001 0.0004 0.001 0.0003 0.0004 0.0118 0.0001 0.0023 0.0011 0.0001 0.0011
Vacuole 0.0034 0.0001 0.003 0.0122 0.0111 0.0002 0.0007 0.0002 0.0003 0.0008 0.0005 0.0005 0.0033 0.0001 0.0027 0.0033 0.0003 0.0005
Vacuole Periphery 0.0014 0 0.0008 0.0035 0.0019 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0026 0 0.0005 0.0008 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 71.255 127.3952 112.6052 137.2585 111.4057 76.452 148.462 170.3592 162.1692 118.9406
Translational Efficiency 2.0251 2.2287 1.8373 1.8462 1.9186 2.0288 1.5911 1.8441 1.7077 1.5375

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2028 499 723 1595 1724 2227 429 1383 3752 2726 1152 2978

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2259.20 2382.85 2753.21 3939.97 1999.58 2290.78 3445.73 3003.24 2139.91 2307.63 3011.10 3504.95
Standard Deviation 394.39 479.67 482.67 1206.67 374.82 445.20 730.22 1384.80 406.65 453.11 675.92 1374.29
Intensity Change Log 2 — 0.076876 0.285303 0.802373 — 0.196142 0.785113 0.586823 — 0.134103 0.541581 0.705163

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000020 0.000162 0.000155 0.000251 0.000145 0.000127 0.000225 0.000336 0.000077 0.000133 0.000181 0.000290
Bud Neck 0.000191 0.000587 0.001220 0.001654 0.000393 0.000488 0.002808 0.001399 0.000283 0.000506 0.001811 0.001535
Bud Site 0.000743 0.001732 0.001358 0.007043 0.001364 0.002620 0.001719 0.005839 0.001028 0.002457 0.001493 0.006484
Cell Periphery 0.000029 0.000234 0.002467 0.003655 0.000167 0.000105 0.001010 0.003611 0.000092 0.000129 0.001924 0.003635
Cytoplasm 0.845381* 0.810637* 0.593956* 0.788970* 0.870800* 0.818722* 0.472771* 0.758185* 0.857061* 0.817242* 0.548827* 0.774674*
Cytoplasmic Foci 0.002866 0.012308 0.012215 0.005001 0.008040 0.006566 0.016077 0.010971 0.005243 0.007617 0.013653 0.007774
Eisosomes 0.000001 0.000001 0.000002 0.000003 0.000001 0.000001 0.000003 0.000003 0.000001 0.000001 0.000002 0.000003
Endoplasmic Reticulum 0.000029 0.000082 0.000046 0.000062 0.000166 0.000165 0.000085 0.000064 0.000092 0.000150 0.000060 0.000063
Endosome 0.000026 0.000277 0.000080 0.000834 0.000234 0.001393 0.000274 0.000317 0.000122 0.001189 0.000152 0.000594
Golgi 0.000158 0.001205 0.000428 0.002756 0.001283 0.001987 0.000674 0.001896 0.000675 0.001844 0.000520 0.002357
Lipid Particles 0.000072 0.000580 0.000882 0.004091 0.000557 0.000422 0.002438 0.004130 0.000295 0.000451 0.001461 0.004109
Mitochondria 0.000091 0.000060 0.000170 0.000481 0.000091 0.000157 0.000249 0.001311 0.000091 0.000139 0.000199 0.000867
Mitotic Spindle 0.000605 0.001304 0.001663 0.000956 0.002348 0.002857 0.001448 0.002723 0.001406 0.002573 0.001583 0.001776
None 0.023131 0.013299 0.010098 0.006725 0.009283 0.016392 0.010876 0.008151 0.016768 0.015825 0.010388 0.007387
Nuclear Periphery 0.000025 0.000077 0.000244 0.000185 0.000077 0.000067 0.000558 0.000203 0.000049 0.000069 0.000361 0.000194
Nuclear Periphery Foci 0.000390 0.003021 0.001112 0.001913 0.003474 0.001836 0.002492 0.002055 0.001807 0.002053 0.001626 0.001979
Nucleolus 0.000072 0.000078 0.000087 0.000220 0.000085 0.000132 0.000124 0.000154 0.000078 0.000122 0.000101 0.000189
Nucleus 0.124170 0.148174* 0.362463* 0.129371 0.096251 0.139664* 0.462837* 0.166014* 0.111341 0.141222* 0.399842* 0.146388*
Peroxisomes 0.000024 0.000018 0.000011 0.000058 0.000015 0.000048 0.000016 0.000066 0.000020 0.000043 0.000013 0.000062
Vacuole 0.001894 0.005489 0.011257 0.043513 0.004100 0.005150 0.023177 0.031619 0.002908 0.005212 0.015696 0.037989
Vacuole Periphery 0.000082 0.000676 0.000086 0.002256 0.001127 0.001100 0.000139 0.000953 0.000562 0.001023 0.000106 0.001651

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.82 -11.44 -5.22 -0.99 0.81 0.26 -7.10 -1.91 -1.95 1.06 -3.91 -12.47 -4.33 -2.63 1.13
Bud Neck -6.45 -11.82 -8.21 -4.65 1.54 -1.01 -8.17 -9.15 -8.54 5.29 -4.77 -12.22 -12.09 -9.71 5.14
Bud Site -4.16 -6.38 -6.25 -4.88 -4.55 -2.31 -7.18 -5.34 -3.68 -2.40 -3.59 -9.30 -8.18 -5.57 -5.28
Cell Periphery -7.30 -2.75 -8.79 -7.91 -1.79 3.71 -9.84 -5.50 -5.65 -2.56 -3.75 -3.94 -9.88 -9.70 -2.54
Cytoplasm 6.73 35.90 16.37 5.63 -20.18 11.77 43.42 22.48 13.80 -26.09 12.49 52.98 27.14 15.07 -30.54
Cytoplasmic Foci -7.23 -12.29 -6.38 4.67 9.22 1.86 -8.77 -3.64 -4.99 6.89 -4.48 -14.48 -6.64 -1.37 10.94
Eisosomes -4.71 -10.63 -5.85 -3.74 0.24 -1.29 -10.71 -4.64 -4.15 5.86 -3.87 -14.61 -7.47 -5.93 4.00
Endoplasmic Reticulum -3.76 -7.20 -3.06 2.30 2.96 0.25 1.13 4.11 2.39 2.85 -2.02 -0.99 2.39 3.08 3.89
Endosome -3.51 -7.91 -2.37 -1.05 -1.73 -3.31 -1.52 -1.58 2.87 0.20 -3.86 -3.25 -2.72 2.20 -1.71
Golgi -3.16 -7.75 -3.87 -1.34 -2.93 -1.12 2.54 -0.43 0.63 -1.83 -2.86 0.11 -3.43 -0.08 -3.53
Lipid Particles -5.41 -8.45 -8.64 -6.57 -4.30 1.39 -5.17 -5.88 -6.15 0.07 -3.07 -8.44 -10.26 -9.50 -3.00
Mitochondria 4.34 -9.44 -2.23 -2.44 -1.15 -1.32 -8.50 -1.25 -1.16 -0.91 -1.27 -11.71 -1.69 -1.53 -1.16
Mitotic Spindle -1.54 -2.17 -1.74 -0.10 1.28 -0.75 -0.10 -1.71 -1.14 -1.71 -2.03 -1.76 -2.30 -0.20 -0.23
None 6.82 12.64 13.05 4.45 3.19 -6.54 0.37 2.45 10.62 2.42 1.90 10.63 12.51 11.44 4.04
Nuclear Periphery -8.39 -15.89 -10.09 -5.31 7.51 0.86 -14.23 -9.47 -9.64 10.86 -2.92 -19.84 -13.84 -10.70 12.65
Nuclear Periphery Foci -6.30 -10.63 -11.18 3.46 0.14 4.17 -0.29 3.98 -0.84 2.80 -1.80 -3.30 -1.52 0.86 2.35
Nucleolus -2.36 -6.73 -2.22 -1.90 -1.35 -0.86 -4.07 -3.40 -1.75 0.06 -1.38 -5.42 -3.50 -1.75 -1.50
Nucleus -4.45 -32.04 -6.53 -0.60 24.41 -12.54 -32.03 -15.61 -6.61 22.63 -11.11 -45.05 -15.02 -5.36 32.86
Peroxisomes -0.03 0.21 -2.09 -2.36 -2.62 -2.57 -4.30 -2.45 -0.71 -1.37 -2.23 -1.94 -3.17 -0.75 -2.54
Vacuole -6.47 -11.37 -19.38 -15.54 -8.51 -1.07 -11.68 -14.21 -13.67 2.80 -4.86 -15.46 -23.79 -21.35 -3.65
Vacuole Periphery -1.09 -1.02 -4.21 -1.68 -4.15 0.15 1.91 0.65 0.74 -4.32 -1.15 1.87 -2.18 -0.80 -5.35
Standard name
Human Ortholog
Description Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Aha1

Aha1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aha1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available