Standard name | |
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Description | Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 8 | 11 | 6 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 5 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 3 | 3 | 2 | 2 | 12 | 9 | 8 | 6 | 1 | 6 | 2 | 4 | 3 | 13 | 14 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 177 | 177 | 165 | 171 | 209 | 231 | 311 | 213 | 221 | 179 | 129 | 135 | 178 | 132 | 192 | 245 | 301 | 214 | 175 | 165 | 158 | 117 | 217 | 187 |
Endoplasmic Reticulum | 0 | 0 | 1 | 2 | 1 | 6 | 17 | 17 | 10 | 16 | 7 | 5 | 0 | 0 | 2 | 6 | 13 | 7 | 0 | 0 | 0 | 0 | 0 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 6 | 1 | 10 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 9 | 5 | 9 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 9 | 8 | 4 | 4 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 4 | 17 | 14 | 20 | 23 | 30 | 42 | 31 | 43 | 2 | 0 | 0 | 7 | 11 | 6 | 0 | 1 | 1 | 0 | 2 | 1 |
Unique Cell Count | 177 | 178 | 167 | 178 | 222 | 252 | 359 | 269 | 285 | 253 | 183 | 209 | 178 | 133 | 196 | 255 | 316 | 219 | 179 | 169 | 175 | 118 | 225 | 201 |
Labelled Cell Count | 177 | 181 | 171 | 180 | 229 | 255 | 380 | 281 | 307 | 265 | 188 | 221 | 182 | 137 | 199 | 273 | 341 | 231 | 179 | 169 | 175 | 118 | 225 | 201 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.0 | 34.6 | 27.7 | 25.2 | 27.6 | 26.2 | 27.8 | 27.3 | 26.6 | 27.1 | 25.4 | 26.9 | 28.2 | 32.5 | 35.4 | 56.6 | 59.4 | 55.9 | 28.3 | 32.5 | 38.4 |
Std Deviation (1e-4) | 5.0 | 6.0 | 6.8 | 6.2 | 9.0 | 6.4 | 7.5 | 7.7 | 8.8 | 8.7 | 8.8 | 9.1 | 6.3 | 7.5 | 9.5 | 23.6 | 23.4 | 22.4 | 6.9 | 8.7 | 10.5 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.0 | -0.08 | 0.01 | -0.02 | -0.05 | -0.03 | -0.12 | -0.04 | 0.03 | 0.23 | 0.36 | 1.03 | 1.1 | 1.01 | 0.03 | 0.23 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 1.5 | 0 |
Cytoplasm | -1.6 | -2.4 | -3.1 | -4.4 | -5.9 | -6.2 | -7.3 | -7.2 | -8.2 | 1.5 | 0.4 | -0.6 | -1.6 | -2.0 | -0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.4 | 2.9 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.3 | 2.7 | 2.7 | 3.5 | 4.0 | 5.3 | 5.3 | 6.0 | 0 | 0 | 0 | 0 | 0 | 0 |