Standard name
Description Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.96 0.94 0.92 0.87 0.79 0.78 0.71 0.7 0.65 1.0 0.99 0.98 0.96 0.95 0.98 0.98 0.98 0.9 1.0 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.06 0.06 0.09 0.11 0.17 0.17 0.21 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 8 11 6 3 7 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 0 5 0 1 3 0 0 0 0 1 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 3 2 2 12 9 8 6 1 6 2 4 3 13 14 1 0 0 0 0 0 0
Cytoplasm 177 177 165 171 209 231 311 213 221 179 129 135 178 132 192 245 301 214 175 165 158 117 217 187
Endoplasmic Reticulum 0 0 1 2 1 6 17 17 10 16 7 5 0 0 2 6 13 7 0 0 0 0 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 2 2 8 6 1 10 0 1 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 4 1 9 5 9 3 0 0 0 1 0 0 0 0 1 0 0 0
Nucleus 0 0 1 0 0 2 9 8 4 4 5 7 0 0 0 1 0 1 0 0 3 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 2 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 4 17 14 20 23 30 42 31 43 2 0 0 7 11 6 0 1 1 0 2 1
Unique Cell Count 177 178 167 178 222 252 359 269 285 253 183 209 178 133 196 255 316 219 179 169 175 118 225 201
Labelled Cell Count 177 181 171 180 229 255 380 281 307 265 188 221 182 137 199 273 341 231 179 169 175 118 225 201


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.0 34.6 27.7 25.2 27.6 26.2 27.8 27.3 26.6 27.1 25.4 26.9 28.2 32.5 35.4 56.6 59.4 55.9 28.3 32.5 38.4
Std Deviation (1e-4) 5.0 6.0 6.8 6.2 9.0 6.4 7.5 7.7 8.8 8.7 8.8 9.1 6.3 7.5 9.5 23.6 23.4 22.4 6.9 8.7 10.5
Intensity Change (Log2) – – – -0.14 -0.0 -0.08 0.01 -0.02 -0.05 -0.03 -0.12 -0.04 0.03 0.23 0.36 1.03 1.1 1.01 0.03 0.23 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.7 1.5 0
Cytoplasm -1.6 -2.4 -3.1 -4.4 -5.9 -6.2 -7.3 -7.2 -8.2 1.5 0.4 -0.6 -1.6 -2.0 -0.8
Endoplasmic Reticulum 0 0 0 2.4 2.9 0 2.9 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.3 2.7 2.7 3.5 4.0 5.3 5.3 6.0 0 0 0 0 0 0