Standard name
Human Ortholog
Description Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.98 0.98 0.93 0.92 0.84 0.87 0.85 0.74 0.79 0.99 0.98 0.99 0.93 0.94 0.97 0.8 0.83 0.79 0.85 0.85 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.11 0.05 0.21 0.23 0.31 0.42 0.39 0.49 0.41 0 0 0 0 0 0 0.06 0.05 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 4 2 2 4
Bud 0 0 0 2 2 1 1 6 2 12 5 10 0 1 2 0 0 0 3 4 0 7 8 8
Bud Neck 3 7 2 0 5 4 5 5 4 2 2 1 0 0 0 0 0 2 0 3 1 0 2 1
Bud Site 0 0 0 0 0 0 0 3 0 2 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 2 1 3 2 0 1 3 3 5 2 4 3 1 0 8 3 1 0 2 3 1 1 3
Cytoplasm 355 439 345 327 371 488 500 658 350 391 291 399 249 320 349 260 189 170 299 343 252 189 273 273
Endoplasmic Reticulum 1 2 3 0 0 0 0 1 0 0 1 0 1 2 2 20 9 9 3 1 2 0 2 2
Endosome 3 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 1 0 8 6 1 2 2 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 2 0 1 1 2 2
Mitochondria 5 10 4 36 20 110 126 245 170 181 193 208 3 0 5 7 7 1 24 19 31 6 12 12
Nucleus 0 1 0 1 1 1 0 7 3 1 3 1 0 2 1 7 1 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3 1 2 1 0 0 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 1 0 0 0 0
SpindlePole 1 0 0 0 0 1 2 5 1 2 0 3 0 0 0 1 1 0 13 6 2 3 3 2
Vac/Vac Membrane 1 3 0 0 1 0 4 4 1 0 7 5 0 1 0 11 8 2 1 4 4 1 2 2
Unique Cell Count 366 452 351 335 377 523 544 781 403 459 395 504 251 326 351 279 201 175 377 413 319 223 321 323
Labelled Cell Count 374 465 355 369 402 605 639 940 535 598 507 634 256 329 361 321 222 188 377 413 319 223 321 323


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.3 3.2 3.0 3.5 3.1 3.0 3.1 2.8 2.7 2.8 2.7 4.2 4.5 4.1 7.5 8.6 7.5 4.4 4.5 4.9
Std Deviation (1e-4) 0.9 0.9 0.8 0.9 1.4 1.0 1.0 1.0 1.1 0.9 1.0 1.0 1.0 1.5 1.3 4.8 6.5 5.1 1.8 2.0 1.8
Intensity Change (Log2) – – – -0.1 0.13 -0.05 -0.09 -0.06 -0.19 -0.26 -0.2 -0.26 0.39 0.48 0.34 1.21 1.42 1.21 0.46 0.47 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9825 2.4183 2.0616 1.6656 1.3978 2.0193 0.2013 -0.8665 -0.607 -0.81 -0.8871 -0.9644 2.5054 3.1998 2.5822 3.2664 2.3424 2.955
Actin 0.0001 0.0003 0.0004 0.0004 0.0029 0.0019 0.011 0.0047 0.0283 0.0009 0.0044 0.0022 0.0448 0.0016 0.0008 0.0011 0.007 0.0005
Bud 0 0.0005 0.0002 0.0007 0.0072 0.0001 0.0008 0.0054 0.0023 0.003 0.0006 0.0026 0.0055 0.0051 0.0006 0.0008 0.0072 0.0015
Bud Neck 0.0001 0.0002 0.0001 0.0003 0.0002 0.0025 0.0032 0.0004 0.0012 0.0008 0.0004 0.0006 0.0024 0.0001 0.0001 0.0002 0.0008 0.0004
Bud Periphery 0 0.0015 0.0003 0.0009 0.0667 0.0001 0.0013 0.0035 0.0024 0.0068 0.0008 0.0051 0.0063 0.0051 0.0007 0.0027 0.0027 0.009
Bud Site 0.0002 0.0031 0.0008 0.0002 0.0034 0 0.0039 0.0173 0.0051 0.0032 0.0009 0.0005 0.0102 0.0251 0.0025 0.0001 0.0036 0.0004
Cell Periphery 0.0004 0.0012 0.0005 0.0002 0.0147 0.0001 0.0004 0.0004 0.0003 0.0004 0.0001 0.0001 0.0009 0.0005 0.0001 0.0002 0.001 0.0001
Cytoplasm 0.3495 0.5353 0.4649 0.4024 0.2065 0.5939 0.2652 0.3726 0.3333 0.4547 0.4321 0.401 0.1443 0.2185 0.1654 0.1232 0.2447 0.171
Cytoplasmic Foci 0.0037 0.0041 0.0061 0.005 0.0021 0.0042 0.0152 0.0139 0.0122 0.0106 0.0323 0.0196 0.0371 0.0029 0.0076 0.0015 0.0217 0.0042
Eisosomes 0.0001 0.0001 0.0001 0.0002 0.0007 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0.0016 0.0002 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0034 0.0043 0.0025 0.0033 0.002 0.0014 0.0089 0.0024 0.0036 0.0025 0.0036 0.0017 0.0082 0.0028 0.002 0.0008 0.0026 0.0005
Endosome 0.0016 0.0048 0.0022 0.002 0.0013 0.0015 0.0273 0.0065 0.008 0.014 0.0169 0.0045 0.0129 0.0014 0.0011 0.0001 0.0313 0.0011
Golgi 0.0001 0.0003 0.0002 0.0001 0.0009 0.0002 0.0039 0.0007 0.0023 0.0009 0.0049 0.0013 0.0099 0.0001 0.0003 0.0001 0.0044 0.0001
Lipid Particles 0.0003 0.0003 0.0009 0.0002 0.0002 0.0002 0.0098 0.0012 0.0015 0.0027 0.0092 0.0025 0.01 0.0004 0.0004 0.0001 0.0069 0.0002
Mitochondria 0.0001 0.0051 0.0003 0.0004 0.0268 0.0005 0.0041 0.0009 0.0016 0.0008 0.0016 0.0014 0.0076 0.0002 0.0004 0.0003 0.0018 0.0002
None 0.6117 0.4335 0.5163 0.5774 0.5171 0.3902 0.4986 0.5562 0.5803 0.4731 0.4595 0.5442 0.6667 0.7337 0.8144 0.8675 0.6453 0.8091
Nuclear Periphery 0.0003 0.0009 0.0006 0.001 0.0004 0.0003 0.0484 0.0014 0.0031 0.0042 0.0078 0.0016 0.003 0.0004 0.0006 0.0002 0.0028 0.0002
Nucleolus 0.0003 0.0001 0.0002 0.0002 0.0018 0.0001 0.0038 0.0005 0.0005 0.0005 0.0007 0.0002 0.003 0.0001 0.0001 0.0001 0.0012 0.0001
Nucleus 0.0271 0.0013 0.0011 0.0025 0.1422 0.0009 0.0595 0.0034 0.005 0.0056 0.003 0.0059 0.002 0.0005 0.0009 0.0003 0.0027 0.0005
Peroxisomes 0 0 0.0002 0.0002 0.0001 0.0001 0.0021 0.0026 0.0013 0.0008 0.0033 0.0008 0.0142 0.0001 0.0003 0.0001 0.003 0.0001
Punctate Nuclear 0.0001 0.0003 0.0006 0.0007 0.0003 0.0003 0.0219 0.0035 0.0038 0.0059 0.0147 0.0026 0.0058 0.0003 0.001 0.0003 0.0061 0.0005
Vacuole 0.0008 0.0023 0.0015 0.0015 0.0021 0.0012 0.0074 0.002 0.0032 0.0073 0.0024 0.0012 0.003 0.0004 0.0005 0.0002 0.0025 0.0003
Vacuole Periphery 0.0001 0.0003 0.0001 0.0002 0.0005 0.0002 0.0031 0.0003 0.0006 0.0014 0.0007 0.0004 0.0007 0.0001 0.0001 0 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5282 12.1683 11.9778 16.0976 7.2035 10.2728 15.6303 13.8964 17.5595 11.5051
Translational Efficiency 0.4977 0.4741 0.5569 0.4413 0.386 0.4227 0.3732 0.5411 0.4598 0.3881

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
Localization
Cell Percentages cytoplasm (25%), nucleus (6%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Adr1

Adr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available