Standard name
Description Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.11 0.16 0.12 0.14 0.07 0.09 0.07 0 0 0 0.08 0 0 0.05 0.24 0.31 0.24 0 0.06 0.05 0.05 0.13 0.09
Bud 0 0 0 0 0 0.05 0 0 0.06 0.07 0.08 0.06 0 0 0 0 0.08 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.08 0.1 0.33 0.29 0.24 0 0 0 0 0.08 0.08
Cytoplasm 0.2 0.33 0.43 0.51 0.49 0.49 0.43 0.48 0.5 0.46 0.41 0.46 0.15 0.26 0.29 0.34 0.25 0.39 0 0.06 0.05 0 0 0
Endoplasmic Reticulum 0.74 0.46 0.34 0.24 0.25 0.21 0.29 0.14 0.08 0.08 0 0.16 0.81 0.53 0.59 0.07 0.12 0.05 0.84 0.59 0.64 0.71 0.67 0.67
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Mitochondria 0.08 0.16 0 0.09 0.06 0.19 0.23 0.21 0.39 0.45 0.52 0.27 0.08 0 0 0 0.1 0.05 0 0.15 0.15 0.1 0.07 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.05 0.05 0.08 0.06 0.05 0.12 0 0.06 0.1 0.05 0 0 0 0.09 0 0.05 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 26 32 62 41 60 34 60 39 9 14 14 38 10 12 13 16 16 9 9 22 28 9 44 25
Bud 2 10 9 9 11 23 31 23 35 34 39 29 1 3 2 0 4 0 0 1 2 1 1 1
Bud Neck 0 0 4 0 3 2 1 1 0 2 1 1 0 0 0 0 0 0 0 6 9 2 3 2
Bud Site 1 0 0 0 0 0 1 0 2 1 3 1 0 0 0 0 0 0
Cell Periphery 3 1 24 18 11 16 16 18 13 13 10 14 8 22 28 22 15 9 0 2 12 7 28 23
Cytoplasm 55 93 161 178 216 248 302 267 272 227 188 219 37 71 81 23 13 15 9 21 27 6 3 3
Endoplasmic Reticulum 200 129 129 82 110 107 199 76 46 41 10 78 204 144 163 5 6 2 262 207 379 128 227 190
Endosome 0 10 1 11 9 12 4 1 16 6 3 4 5 18 11 2 1 2 0 6 3 0 0 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 3 3 2 2 1 0 1
Mitochondria 21 45 1 31 28 98 160 117 213 222 239 128 21 11 9 3 5 2 11 53 90 19 22 21
Nucleus 1 0 1 0 2 0 2 9 3 7 9 4 1 1 0 1 1 1 0 0 0 0 0 1
Nuclear Periphery 3 1 0 0 1 0 2 4 0 6 4 0 9 0 3 0 0 0 2 2 4 0 0 0
Nucleolus 0 0 1 0 0 0 0 2 1 3 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 1 1 3 0 0 0 6 1 1 0 0 0 1 1 0 0 0
SpindlePole 2 0 4 3 7 5 9 8 5 10 1 13 0 4 0 3 4 0 0 1 2 0 0 0
Vac/Vac Membrane 1 14 18 17 34 32 38 64 23 29 44 24 4 10 7 6 2 2 7 16 23 2 3 5
Unique Cell Count 269 280 376 346 438 507 697 552 545 495 463 475 251 270 275 67 52 38 311 350 596 182 340 283
Labelled Cell Count 315 336 415 390 492 578 825 630 639 618 568 553 300 303 318 83 68 45 311 350 596 182 340 283


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.4 6.2 5.9 6.3 5.1 5.5 5.7 4.8 4.7 4.4 5.3 6.2 6.6 6.5 9.4 8.7 9.6 5.7 5.8 6.4
Std Deviation (1e-4) 0.7 0.8 0.7 0.7 1.0 0.7 0.9 1.0 0.8 1.0 0.7 0.9 0.8 1.2 1.0 1.5 1.6 2.3 1.0 1.0 1.3
Intensity Change (Log2) -0.08 0.02 -0.28 -0.19 -0.13 -0.38 -0.4 -0.51 -0.24 -0.01 0.07 0.06 0.6 0.48 0.62 -0.14 -0.1 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.8 -1.1 -4.6 -3.9 -4.5 0 0 0 -3.8 -4.8 -4.7 -4.6 1.5 2.5 1.1
Bud 0.2 0.1 1.7 1.7 1.5 0 0 0 2.6 0 -1.2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -2.7 -2.3 -3.4 -2.2 0 0 0 -2.4 -1.8 0.9 1.8 6.5 0 0
Cytoplasm 2.3 1.9 1.8 0.2 1.7 2.1 0.9 -0.6 1.0 -7.4 -4.3 -3.5 -1.3 -2.5 -0.4
Endoplasmic Reticulum -3.1 -2.9 -4.4 -2.0 -7.4 0 0 0 -6.0 11.5 4.8 6.3 -4.4 -3.3 -3.7
Endosome 3.1 0 2.6 0 0 0 0 0 0 0 4.7 3.5 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.7 4.7 8.9 9.9 9.4 0 0 0 10.8 5.4 3.5 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.7 0 0 0 0 0 0 1.7 0 0 0 0 0 0
Vacuole 0.1 1.7 1.0 0.5 3.6 -0.4 0.7 2.6 0.2 -2.1 -0.7 -1.5 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available