ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.97 0.99 0.93 0.83 0.79 0.78 0.74 0.7 0.54 0.6 0.99 1.0 1.0 0.96 0.94 0.94 0.82 0.87 0.67 0.87 0.89 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.09 0.09 0.34 0.38 0.36 0.41 0.52 0.64 0.57 0 0 0 0 0 0.05 0.07 0 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 5 9 2 2 2
Bud 1 1 0 0 3 1 6 6 6 11 4 11 0 0 0 0 0 0 0 2 7 4 3 7
Bud Neck 0 1 2 0 4 2 2 0 1 6 0 3 1 6 4 0 2 2 2 0 2 2 0 3
Bud Site 0 0 0 0 0 0 0 2 2 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 0 0 1 3 1 0 1 2 1 0 5 1 7 4 9 4 1 4 0 2 1
Cytoplasm 408 223 293 159 237 241 311 260 304 304 128 196 321 522 506 184 269 208 333 208 211 207 302 264
Endoplasmic Reticulum 2 3 2 0 1 2 0 0 0 1 1 1 1 2 1 6 14 14 2 1 5 0 0 2
Endosome 0 0 0 1 0 0 1 0 0 1 0 0 1 0 1 3 3 2 4 2 5 1 1 2
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 0 3 1 0 0
Mitochondria 37 6 6 14 24 100 149 119 169 228 153 188 0 0 0 0 9 12 27 10 43 5 8 12
Nucleus 0 0 1 1 2 0 0 1 2 0 2 3 2 0 1 1 1 0 0 0 1 0 1 0
Nuclear Periphery 1 0 0 0 0 1 1 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 1 3 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 2 2 12 12 17 6 2 7 0 0 0 0 5 1 2 1 5 3 4 7
Vac/Vac Membrane 0 2 0 0 2 2 2 1 2 5 2 6 0 0 0 2 5 5 3 2 3 1 3 4
Unique Cell Count 427 230 302 160 256 291 396 334 413 437 238 327 323 524 508 191 285 222 406 238 315 238 339 320
Labelled Cell Count 451 237 310 176 275 352 488 405 504 564 297 418 327 535 514 206 315 253 406 238 315 238 339 320


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 3.8 3.6 3.3 2.8 2.9 2.8 2.8 2.6 2.4 2.5 5.5 5.1 5.0 5.7 4.9 5.0 5.7 5.5 5.9
Std Deviation (1e-4) 0.6 0.7 1.5 1.9 1.3 1.7 2.1 1.5 1.4 1.2 0.9 1.0 1.2 0.9 1.0 2.2 1.6 1.5 1.7 1.2 1.5
Intensity Change (Log2) – – – -0.07 -0.19 -0.4 -0.36 -0.4 -0.42 -0.53 -0.66 -0.59 0.54 0.44 0.41 0.61 0.39 0.41 0.59 0.56 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.098 1.6956 1.8165 1.6943 0.6772 1.4583 1.5988 0.5174 0.6598 0.6253 0.5499 0.273 -0.3283 -0.4118 -0.0901 0.1474 -0.102 -0.3135
Actin 0.0184 0.0018 0.0103 0.0048 0.0013 0.0061 0.052 0.0078 0.0255 0.007 0.0186 0.0018 0.0566 0.0013 0.0029 0.0038 0.054 0.0021
Bud 0.0009 0.0006 0.0008 0.0013 0.0009 0.0007 0.0063 0.0037 0.0003 0.0008 0.0027 0.0002 0.0037 0.0007 0.0092 0.0031 0.0004 0.0002
Bud Neck 0.0012 0.0003 0.0015 0.0004 0.0006 0.0057 0.0025 0.0003 0.001 0.0275 0.0007 0.0004 0.0029 0.0001 0.0003 0.0006 0.0008 0.0005
Bud Periphery 0.0026 0.0014 0.001 0.0031 0.0014 0.0017 0.0143 0.0019 0.0003 0.0027 0.01 0.0005 0.0042 0.0016 0.004 0.0039 0.0007 0.0006
Bud Site 0.0031 0.002 0.0062 0.0007 0.0012 0.0004 0.01 0.011 0.0066 0.0079 0.0067 0.0001 0.0072 0.0009 0.0108 0.0055 0.0014 0.0002
Cell Periphery 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0046 0.0004 0.0001 0.0003 0.0003 0 0.0005 0.0002 0.0004 0.0003 0.0002 0
Cytoplasm 0.081 0.1952 0.2071 0.2356 0.1898 0.1296 0.0942 0.1973 0.281 0.2629 0.1554 0.2418 0.104 0.1766 0.2557 0.0965 0.1849 0.237
Cytoplasmic Foci 0.0274 0.0055 0.0095 0.0067 0.0045 0.0131 0.0206 0.0062 0.0119 0.0129 0.0093 0.0174 0.0226 0.0034 0.0076 0.0105 0.0092 0.0054
Eisosomes 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0.001 0.0001 0.0002 0.0002 0.0005 0.0001 0.0005 0.0001 0.0002 0.0002 0.0004 0.0001
Endoplasmic Reticulum 0.0033 0.0012 0.0023 0.0031 0.0011 0.0015 0.0167 0.0019 0.003 0.0022 0.0034 0.0023 0.003 0.0013 0.0025 0.0011 0.0038 0.0035
Endosome 0.0083 0.0021 0.0047 0.0029 0.0006 0.0032 0.0501 0.0012 0.006 0.0148 0.0201 0.006 0.0194 0.0006 0.0043 0.0009 0.0198 0.0113
Golgi 0.0028 0.0001 0.0016 0.0002 0.0001 0.0009 0.0189 0.0002 0.007 0.0072 0.0028 0.0011 0.008 0.0001 0.0019 0.0002 0.0033 0.0049
Lipid Particles 0.007 0.0001 0.0017 0.0003 0.0002 0.0004 0.0257 0.0004 0.0077 0.0175 0.0187 0.0009 0.0147 0.0002 0.004 0.0007 0.0039 0.0041
Mitochondria 0.0105 0.0003 0.0044 0.0007 0.0005 0.001 0.0189 0.0004 0.0022 0.0095 0.0115 0.001 0.0082 0.0003 0.008 0.0013 0.0011 0.0031
None 0.7913 0.7682 0.7227 0.7083 0.7822 0.8197 0.5476 0.7575 0.6278 0.5785 0.7083 0.716 0.7076 0.8101 0.6733 0.8528 0.7008 0.7087
Nuclear Periphery 0.0082 0.0047 0.002 0.0024 0.0016 0.0006 0.0331 0.0012 0.0012 0.0039 0.0091 0.0034 0.0112 0.0005 0.0021 0.0005 0.0066 0.006
Nucleolus 0.001 0.0005 0.0007 0.0003 0.001 0.0003 0.0015 0.0004 0.0002 0.0093 0.0007 0.0002 0.0007 0.0001 0.0006 0.0019 0.0002 0.0002
Nucleus 0.0048 0.008 0.0115 0.0245 0.0074 0.0015 0.0389 0.0027 0.0075 0.0085 0.008 0.0013 0.0017 0.0007 0.0059 0.0039 0.0016 0.0013
Peroxisomes 0.0199 0.002 0.0025 0.0007 0.0003 0.0062 0.0184 0.0002 0.0074 0.0127 0.0053 0.0005 0.0063 0.0001 0.0013 0.0047 0.0015 0.008
Punctate Nuclear 0.0052 0.0019 0.0077 0.002 0.0043 0.0069 0.0072 0.0033 0.0018 0.0086 0.0052 0.0035 0.0127 0.0005 0.0031 0.005 0.0036 0.0013
Vacuole 0.0015 0.0024 0.0013 0.0017 0.0006 0.0003 0.0141 0.0017 0.001 0.003 0.0018 0.0009 0.0023 0.0006 0.0014 0.0024 0.0013 0.0006
Vacuole Periphery 0.001 0.0012 0.0003 0.0001 0.0002 0.0001 0.0036 0.0002 0.0001 0.0021 0.0008 0.0004 0.0019 0.0001 0.0005 0.0002 0.0006 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.3655 21.3399 6.4513 7.0297 23.4996 22.9749 22.5756 12.1357 7.1794 22.9855
Translational Efficiency 1.5361 1.7664 0.7446 0.8338 1.5489 2.1914 1.2133 0.6834 1.2458 1.1425

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
256 161 335 1216 1547 1817 917 1096 1803 1978 1252 2312

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.75 751.30 891.46 1265.38 638.28 698.75 931.75 1190.11 639.48 703.03 920.97 1229.70
Standard Deviation 68.58 95.08 103.18 304.54 79.70 78.66 161.32 292.74 78.28 81.40 149.09 301.35
Intensity Change Log 2 — 0.216181 0.462962 0.968291 — 0.130587 0.545753 0.898834 — 0.174301 0.504679 0.934209

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000114 0.001371 0.000657 0.023091 0.000096 0.000602 0.001566 0.014126 0.000098 0.000664 0.001323 0.018841
Bud Neck 0.011905 0.035912 0.005336 0.040997 0.008716 0.036151 0.006488 0.027677 0.009169 0.036132 0.006180 0.034683
Bud Site 0.001822 0.016166 0.007821 0.096854 0.003130 0.016467 0.010518 0.060225 0.002944 0.016442 0.009796 0.079490
Cell Periphery 0.000140 0.000283 0.000219 0.000666 0.000125 0.000196 0.000368 0.000332 0.000127 0.000203 0.000328 0.000508
Cytoplasm 0.389939* 0.270833* 0.134217* 0.108718* 0.568391* 0.400295* 0.350253* 0.149021* 0.543054* 0.389757* 0.292448* 0.127824*
Cytoplasmic Foci 0.311410* 0.347251* 0.007995 0.072246 0.167289 0.260237* 0.033941 0.076236 0.187752* 0.267319* 0.026999 0.074137
Eisosomes 0.000193 0.000317 0.000094 0.000086 0.000103 0.000217 0.000104 0.000058 0.000115 0.000225 0.000101 0.000073
Endoplasmic Reticulum 0.000383 0.002851 0.009241 0.006030 0.000428 0.000886 0.006632 0.004189 0.000421 0.001046 0.007330 0.005157
Endosome 0.008353 0.029746 0.003309 0.043921 0.004316 0.019206 0.011133 0.044910 0.004889 0.020064 0.009039 0.044390
Golgi 0.000783 0.005439 0.000105 0.017109 0.001098 0.006487 0.000361 0.011427 0.001053 0.006402 0.000292 0.014415
Lipid Particles 0.010311 0.013429 0.003601 0.038870 0.004582 0.006964 0.007059 0.049501 0.005396 0.007490 0.006134 0.043909
Mitochondria 0.008213 0.005631 0.000890 0.006548 0.002414 0.013094 0.000617 0.006637 0.003237 0.012487 0.000690 0.006590
Mitotic Spindle 0.000592 0.004727 0.001250 0.300768* 0.001357 0.011596 0.017279 0.314599* 0.001249 0.011037 0.012990 0.307324*
None 0.002296 0.001136 0.005680 0.002669 0.007816 0.002251 0.015280 0.008424 0.007032 0.002160 0.012711 0.005397
Nuclear Periphery 0.000542 0.000907 0.002682 0.003374 0.000731 0.000377 0.002141 0.002572 0.000704 0.000420 0.002285 0.002994
Nuclear Periphery Foci 0.000794 0.001048 0.006073 0.035692 0.000300 0.000354 0.010799 0.030265 0.000370 0.000411 0.009534 0.033119
Nucleolus 0.001931 0.002468 0.001985 0.001266 0.001049 0.001401 0.002068 0.001830 0.001174 0.001488 0.002046 0.001533
Nucleus 0.190881* 0.131677* 0.725994* 0.059750 0.203075* 0.129863 0.394845* 0.071085 0.201344* 0.130011* 0.483451* 0.065123
Peroxisomes 0.009420 0.038503 0.000473 0.018420 0.002765 0.022155 0.004245 0.026222 0.003710 0.023486 0.003236 0.022119
Vacuole 0.049463 0.088797 0.082175 0.116415 0.021656 0.069541 0.124024 0.093151 0.025604 0.071109 0.112827 0.105387
Vacuole Periphery 0.000517 0.001508 0.000208 0.006510 0.000564 0.001659 0.000277 0.007514 0.000558 0.001647 0.000259 0.006986

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.79 -4.71 -6.67 -5.16 -5.89 -6.77 -5.73 -5.39 -4.92 -3.65 -6.36 -6.71 -8.56 -7.90 -6.61
Bud Neck -3.52 0.54 -16.06 -5.31 -17.03 -12.54 -0.70 -15.13 -4.96 -14.36 -12.96 -0.58 -23.77 -9.38 -22.79
Bud Site -4.50 -2.71 -22.12 -13.91 -17.91 -9.60 -7.69 -18.13 -12.75 -13.95 -10.41 -7.75 -28.11 -20.23 -22.71
Cell Periphery -1.92 -5.79 -12.99 -6.62 -10.61 -4.62 -13.50 -11.18 -8.42 -3.34 -4.96 -14.75 -16.90 -13.63 -9.84
Cytoplasm 5.02 15.38 16.45 7.11 -0.37 18.06 28.55 43.93 25.24 9.73 17.36 34.41 53.50 31.98 10.57
Cytoplasmic Foci -1.65 21.39 19.01 16.52 -11.08 -12.71 25.19 24.47 37.59 -1.19 -11.22 32.04 30.03 41.74 -4.61
Eisosomes -1.76 4.12 6.32 3.38 3.30 -5.72 -1.60 1.48 11.88 8.20 -5.90 -0.86 2.02 11.67 8.04
Endoplasmic Reticulum -1.57 -14.76 -15.02 -2.12 7.35 -1.32 -18.67 -12.82 -8.51 7.38 -1.81 -23.57 -20.03 -10.90 9.70
Endosome -4.41 2.98 -5.70 2.48 -9.69 -13.84 -6.48 -8.41 1.89 -2.30 -14.24 -5.67 -12.37 2.38 -5.51
Golgi -3.24 3.88 -3.59 0.57 -4.31 -8.23 4.79 -2.51 3.52 -3.53 -8.74 5.73 -4.16 3.13 -5.44
Lipid Particles -0.80 2.98 -4.96 -2.72 -11.58 -4.29 -4.00 -6.99 -5.89 -5.32 -3.26 -1.99 -11.64 -10.13 -10.44
Mitochondria 0.61 1.81 1.26 1.29 -4.44 -8.05 4.76 -0.53 7.02 -2.98 -6.84 3.81 0.39 7.59 -5.15
Mitotic Spindle -1.79 -2.24 -21.62 -19.88 -21.04 -5.27 -6.92 -19.96 -18.52 -15.95 -5.52 -7.04 -29.30 -26.84 -24.08
None 1.85 -2.23 -2.65 -7.47 0.59 5.06 -0.65 0.80 -6.09 1.35 5.11 -0.52 1.76 -7.25 2.14
Nuclear Periphery -0.71 -15.46 -13.77 -7.47 -3.96 0.82 -5.84 -8.07 -16.38 -3.94 0.76 -7.30 -10.41 -20.90 -5.79
Nuclear Periphery Foci -0.56 -2.09 -11.51 -11.63 -4.60 -0.46 -9.01 -9.87 -9.83 -0.28 -0.35 -8.83 -15.58 -15.55 -3.16
Nucleolus -0.62 -0.37 -2.76 -2.31 -2.60 -1.86 -3.79 -6.36 -5.92 -4.01 -1.55 -3.62 -8.18 -7.70 -4.81
Nucleus 3.32 -25.18 3.76 -0.85 32.20 11.28 -15.18 4.96 -4.30 17.75 11.80 -24.47 7.68 -3.93 29.66
Peroxisomes -3.82 5.87 1.43 4.21 -6.74 -13.38 -1.94 -4.41 6.57 -2.45 -13.37 0.03 -4.78 9.36 -3.81
Vacuole -3.66 -7.43 -21.68 -11.80 -7.72 -17.71 -23.12 -27.05 -20.10 -2.65 -16.95 -24.52 -39.04 -28.20 -5.65
Vacuole Periphery -3.13 3.24 -3.07 -0.69 -3.90 -8.27 4.87 -2.11 -0.46 -2.58 -8.83 5.72 -3.45 -0.61 -4.30
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (26%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDR222W

YDR222W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr222w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available