ORF
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.97 0.99 0.93 0.83 0.79 0.78 0.74 0.70 0.54 0.60 0.99 1.00 1.00 0.96 0.94 0.94 0.82 0.87 0.67 0.87 0.89 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.09 0.09 0.34 0.38 0.36 0.41 0.52 0.64 0.57 0 0 0 0 0 0.05 0.07 0 0.14 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 5 9 2 2 2
Bud 1 1 0 0 3 1 6 6 6 11 4 11 0 0 0 0 0 0 0 2 7 4 3 7
Bud Neck 0 1 2 0 4 2 2 0 1 6 0 3 1 6 4 0 2 2 2 0 2 2 0 3
Bud Site 0 0 0 0 0 0 0 2 2 1 2 0 0 0 0 0 0 0
Cell Periphery 0 1 3 0 0 1 3 1 0 1 2 1 0 5 1 7 4 9 4 1 4 0 2 1
Cytoplasm 408 223 293 159 237 241 311 260 304 304 128 196 321 522 506 184 269 208 333 208 211 207 302 264
Endoplasmic Reticulum 2 3 2 0 1 2 0 0 0 1 1 1 1 2 1 6 14 14 2 1 5 0 0 2
Endosome 0 0 0 1 0 0 1 0 0 1 0 0 1 0 1 3 3 2 4 2 5 1 1 2
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 0 3 1 0 0
Mitochondria 37 6 6 14 24 100 149 119 169 228 153 188 0 0 0 0 9 12 27 10 43 5 8 12
Nucleus 0 0 1 1 2 0 0 1 2 0 2 3 2 0 1 1 1 0 0 0 1 0 1 0
Nuclear Periphery 1 0 0 0 0 1 1 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 1 3 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 2 2 12 12 17 6 2 7 0 0 0 0 5 1 2 1 5 3 4 7
Vac/Vac Membrane 0 2 0 0 2 2 2 1 2 5 2 6 0 0 0 2 5 5 3 2 3 1 3 4
Unique Cell Count 427 230 302 160 256 291 396 334 413 437 238 327 323 524 508 191 285 222 406 238 315 238 339 320
Labelled Cell Count 451 237 310 176 275 352 488 405 504 564 297 418 327 535 514 206 315 253 406 238 315 238 339 320

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Punctate Nuclear

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 3.8 3.6 3.3 2.8 2.9 2.8 2.8 2.6 2.4 2.5 5.5 5.1 5.0 5.7 4.9 5.0 5.7 5.5 5.9
Std Deviation (1e-4) 0.6 0.7 1.5 1.9 1.3 1.7 2.1 1.5 1.4 1.2 0.9 1.0 1.2 0.9 1.0 2.2 1.6 1.5 1.7 1.2 1.5
Intensity Change (Log2) -0.07 -0.19 -0.4 -0.36 -0.4 -0.42 -0.53 -0.66 -0.59 0.54 0.44 0.41 0.61 0.39 0.41 0.59 0.56 0.65

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole