Standard name
Description SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some alleles of SIR4 prolong lifespan; required for telomere hypercluster formation in quiescent yeast cells

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.08 0.09 0.09 0.13 0.13 0.12 0.09 0.05 0.06 0.08 0.26 0.31 0.31 0.27 0.24 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.50 0.30 0.09 0.06 0.29 0.05 0 0 0 0 0 0.28 0.35 0.20 0.24 0.31 0.36 0.41 0.28 0.30 0.28 0.37 0.30
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.27 0.51 0.75 0.43 0.74 0.82 0.84 0.92 0.88 0.89 0.14 0.11 0.28 0.12 0 0.15 0 0.08 0.11 0.07 0.06 0.07
Nucleus 0.13 0.17 0.11 0.08 0.05 0.10 0.07 0.10 0.08 0.07 0.07 0.14 0.12 0.10 0.16 0.19 0.13 0.05 0.11 0.05 0.08 0 0
Nuclear Periphery 0.08 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.09 0.08 0.07 0.14 0.13 0.10 0.05 0.08 0.09
Nucleolus 0.08 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.08 0.14 0.15 0.07 0.07 0.07 0.07 0 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.25 0.08 0.23 0.12 0.16 0.05 0.08 0.09 0 0 0.07 0.11 0.11 0.10 0.11 0.14 0.10 0.21 0.18 0.20 0.26 0.28 0.27
Vac/Vac Membrane 0.18 0.28 0.05 0.07 0.11 0 0 0 0 0 0 0.16 0.14 0.12 0.17 0.17 0.16 0 0.05 0.05 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 1 2 4
Bud 0 3 1 5 9 5 6 16 8 7 10 1 0 3 2 0 0 5 3 1 2 2 4
Bud Neck 1 0 2 0 1 0 1 2 1 0 0 0 2 0 1 0 0 1 2 3 2 1 3
Bud Site 0 0 3 5 1 1 10 10 3 5 14 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 31 20 23 32 25 37 30 11 14 23 44 62 69 46 24 19 5 9 2 6 7 6
Endoplasmic Reticulum 0 3 2 2 2 2 1 2 0 1 0 0 2 1 7 6 1 0 2 2 0 1 3
Endosome 152 119 20 15 74 10 8 2 2 3 4 47 70 45 41 32 35 196 152 104 67 124 111
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 0 3 2 1 2 0
Mitochondria 7 109 114 188 109 145 253 294 196 200 255 23 21 62 21 4 15 18 45 39 17 19 24
Nucleus 41 68 24 19 12 19 22 34 16 16 20 23 24 23 27 19 13 22 62 17 20 7 14
Nuclear Periphery 25 13 8 7 5 4 3 5 7 12 6 8 2 2 15 8 7 65 69 34 12 25 34
Nucleolus 25 9 4 4 5 5 4 12 3 12 11 9 4 7 14 14 15 31 39 23 16 10 15
Peroxisomes 1 2 1 0 15 0 0 0 0 0 1 2 5 2 0 0 2 2 2 6 2 6 5
SpindlePole 77 32 50 29 41 9 25 33 4 9 19 19 22 23 19 14 10 99 98 69 61 91 98
Vac/Vac Membrane 56 112 10 18 28 5 12 5 9 7 8 27 27 28 29 17 16 11 29 16 17 10 17
Unique Cell Count 304 397 222 250 254 196 309 349 213 226 288 167 199 225 169 102 98 484 548 346 242 331 365
Labelled Cell Count 405 502 259 315 334 230 382 445 260 286 371 203 241 265 226 139 135 484 548 346 242 331 365

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nuclear Periphery

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 4.4 3.7 3.5 3.9 3.5 3.3 3.2 2.8 3.0 2.8 4.5 4.7 4.3 6.8 7.4 6.9 4.7 4.9 5.2
Std Deviation (1e-4) 0.7 1.0 0.9 1.3 1.4 1.4 1.3 0.9 1.0 1.1 0.9 1.1 1.5 1.5 2.4 2.5 2.3 1.3 1.8 1.4
Intensity Change (Log2) -0.1 0.1 -0.1 -0.2 -0.2 -0.4 -0.3 -0.4 0.3 0.3 0.2 0.9 1.0 0.9 0.3 0.4 0.5

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0 0 0