Standard name
Description RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.07 0 0.09 0 0 0.07 0 0.06 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.06 0 0.15 0 0.07 0.09 0.07 0.36 0.22 0.42 0.17 0.05 0 0 0 0 0 0 0 0
Nucleus 0.97 0.93 0.89 0.92 0.94 0.88 0.94 0.84 0.86 0.86 0.83 0.91 0.83 0.89 0.85 0.95 0.87 0.76 0.69 0.78 0.85
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.08 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Vac/Vac Membrane 0 0 0.07 0 0 0 0 0 0 0 0 0 0.07 0.06 0.07 0 0.07 0.08 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 2 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 3 5 1 3 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 7 1 0 8 10 9 23 4 6 12 5 20 9 12 0 5 1 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 2 0 0 0 0 3 4 4 0 1 0 0 0 0
Endosome 0 0 0 0 0 1 0 1 0 0 0 0 3 2 3 0 4 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2 0 0 0
Mitochondria 21 21 0 4 0 10 19 18 40 31 70 29 15 5 5 0 3 1 0 0 0
Nucleus 131 315 25 24 168 134 194 223 96 121 137 157 277 193 219 123 312 52 13 17 45
Nuclear Periphery 0 0 0 1 0 1 0 2 1 1 1 4 0 2 0 0 2 0 0 0 0
Nucleolus 1 0 0 2 0 1 1 6 5 8 8 5 6 2 5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 2 0 0 1 1 1 0 0 2 0 4 1 1 1 1 0 1
Vac/Vac Membrane 1 15 2 0 2 3 3 2 1 0 2 2 22 12 18 3 23 5 0 0 3
Unique Cell Count 135 338 28 26 178 152 207 264 111 140 166 172 332 217 258 131 360 69 20 22 54
Labelled Cell Count 155 360 28 31 180 160 227 279 151 174 231 206 351 230 270 131 360 69 20 22 54


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.7 6.9 5.5 6.9 5.8 5.7 6.3 4.4 5.0 4.7 5.5 8.8 8.6 8.1 5.6
Std Deviation (1e-4) 0.8 1.0 1.2 1.4 2.2 1.8 1.7 2.4 1.3 1.1 1.6 2.4 2.4 2.1 2.2 0.9
Intensity Change (Log2) -0.34 -0.0 -0.24 -0.27 -0.13 -0.64 -0.45 -0.55 -0.33 0.35 0.32 0.24 -0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 2.8 0 0 0 0 0
Nucleus 0.4 1.0 -0.2 0.9 -0.7 -0.4 -0.4 -0.9 0.3 -0.8 -0.1 -0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available