Standard name
Description Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.29 0.21 0 0.07 0.15 0 0.07 0.07
Bud 0.05 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.34 0.26 0.27 0 0 0 0 0 0
Cytoplasm 0.7 0.91 0.87 0.87 0.8 0.68 0.67 0.61 0.57 0.6 0.47 0.53 0.92 0.92 0.96 0.46 0.39 0.45 0.49 0.39 0.24 0.34 0.4 0.37
Endoplasmic Reticulum 0.15 0.14 0.07 0.06 0.07 0.08 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0.15 0.16 0.28 0.15 0.16 0.15
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.07 0.1 0.35 0.43 0.42 0.51 0.49 0.67 0.6 0 0 0 0.07 0.08 0.09 0.21 0.11 0.17 0.24 0.16 0.13
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0.07 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 2 0 3 0 3 4 2 1 0 2 7 1 31 49 35 1 4 27 7 14 23
Bud 1 0 3 2 4 9 7 14 12 5 10 11 1 2 1 1 6 2 0 0 3 5 3 9
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 3 2 0 2 1 1 2 4 7 8 11
Bud Site 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 1 0 8 8 6 8 17 7 7 4 10 4 10 13 50 44 46 0 0 6 6 2 7
Cytoplasm 14 20 140 118 209 154 294 305 145 219 100 175 212 312 364 68 67 77 25 26 44 61 81 119
Endoplasmic Reticulum 3 3 11 8 18 19 18 15 8 11 9 8 15 18 9 2 6 7 7 11 51 27 31 46
Endosome 0 1 2 1 11 1 3 5 3 2 0 1 1 1 1 1 2 1 0 2 3 2 0 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 3 1 2 2 1 1 4
Mitochondria 0 0 8 9 27 79 190 210 131 179 143 198 0 0 0 10 13 15 10 7 30 44 31 41
Nucleus 1 0 1 0 2 1 3 4 0 5 1 1 0 1 1 3 0 1 0 0 0 1 0 1
Nuclear Periphery 1 0 0 0 0 0 0 3 0 2 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 5 0 0 1 0 1 2 0 0 0 0 0 0 3 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 5 8 0 1 0 3 0 0 0 1 3 1 0 2 0 0 0 3
Vac/Vac Membrane 1 0 1 2 7 4 2 14 7 11 2 5 0 2 3 1 7 14 1 3 4 13 22 33
Unique Cell Count 20 22 161 135 261 226 441 496 255 368 213 332 231 340 381 147 170 170 52 69 182 182 201 318
Labelled Cell Count 21 25 170 151 292 276 532 600 318 447 272 416 236 356 395 169 201 206 52 69 182 182 201 318


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.9 4.8 4.9 4.8 4.3 3.8 4.0 3.6 3.6 3.6 3.5 6.2 5.8 5.9 8.4 8.2 8.3 6.5 6.6 6.6
Std Deviation (1e-4) 0.4 1.6 2.1 2.3 2.1 1.7 1.9 1.5 0.9 1.3 1.3 1.4 1.3 1.2 1.2 1.6 1.6 1.5 1.7 1.9 1.9
Intensity Change (Log2) – – – 0.01 -0.02 -0.17 -0.36 -0.28 -0.43 -0.42 -0.42 -0.46 0.36 0.26 0.28 0.81 0.76 0.79 0.42 0.44 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 5.4 6.7 5.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 8.1 6.9 7.1
Cytoplasm 0.1 -1.8 -4.3 -4.9 -6.0 -6.4 -6.2 -8.0 -7.4 1.6 1.7 3.6 -7.6 -8.9 -8.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.1 -0.7 -2.5 -2.4 -1.4 -1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0.7 1.0 1.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3