ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 72 | 114 | 17 | 38 | 76 | 120 | 194 | 180 | 134 | 179 | 215 | 261 | 22 | 36 | 121 | 51 | 51 | 28 | 7 | 16 | 0 | 0 | 7 | 2 |
Endoplasmic Reticulum | 3 | 5 | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 1 | 8 | 7 | 14 | 0 | 0 | 0 | 0 | 2 | 3 |
Endosome | 24 | 2 | 1 | 2 | 1 | 3 | 5 | 1 | 2 | 1 | 1 | 1 | 2 | 0 | 2 | 5 | 4 | 4 | 18 | 15 | 2 | 5 | 8 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 8 | 3 | 1 | 2 | 0 | 1 | 1 | 2 |
Mitochondria | 8 | 21 | 0 | 0 | 2 | 2 | 1 | 1 | 5 | 5 | 6 | 4 | 0 | 0 | 2 | 3 | 3 | 0 | 2 | 4 | 3 | 0 | 1 | 5 |
Nucleus | 60 | 62 | 36 | 22 | 28 | 36 | 82 | 65 | 36 | 46 | 29 | 20 | 8 | 12 | 21 | 110 | 128 | 80 | 11 | 5 | 0 | 1 | 5 | 7 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 4 | 0 | 10 |
Nucleolus | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 319 | 306 | 61 | 88 | 125 | 233 | 310 | 251 | 178 | 219 | 195 | 250 | 75 | 24 | 48 | 153 | 196 | 160 | 414 | 374 | 118 | 22 | 72 | 116 |
Unique Cell Count | 411 | 403 | 91 | 111 | 189 | 315 | 448 | 369 | 272 | 350 | 364 | 420 | 105 | 65 | 169 | 268 | 323 | 246 | 473 | 428 | 130 | 43 | 110 | 176 |
Labelled Cell Count | 492 | 511 | 116 | 151 | 234 | 395 | 597 | 499 | 355 | 454 | 450 | 542 | 115 | 73 | 195 | 332 | 398 | 291 | 473 | 428 | 130 | 43 | 110 | 176 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 5.7 | 7.5 | 7.9 | 6.6 | 8.4 | 8.5 | 8.1 | 7.4 | 7.6 | 7.2 | 7.6 | 9.2 | 8.7 | 7.9 | 9.7 | 11.8 | 11.8 | 8.5 | 11.5 | 13.1 |
Std Deviation (1e-4) | 1.2 | 1.1 | 1.3 | 1.5 | 1.9 | 1.8 | 1.9 | 1.6 | 1.9 | 1.8 | 1.7 | 1.9 | 2.2 | 2.4 | 2.1 | 3.8 | 3.9 | 3.5 | 2.1 | 3.5 | 3.4 |
Intensity Change (Log2) | – | – | – | 0.08 | -0.18 | 0.16 | 0.17 | 0.1 | -0.03 | 0.02 | -0.06 | 0.02 | 0.29 | 0.21 | 0.08 | 0.38 | 0.66 | 0.66 | 0.18 | 0.62 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.5 | 3.6 | 3.4 | 4.4 | 5.2 | 5.1 | 5.6 | 6.9 | 7.5 | 0.4 | 4.8 | 8.2 | 0.1 | -0.7 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -3.1 | -4.6 | -6.2 | -4.5 | -4.5 | -5.5 | -5.8 | -7.7 | -9.6 | -5.3 | -2.8 | -5.0 | 0.2 | 0 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.0 | -0.1 | 1.3 | 0.4 | 0.2 | -0.3 | -0.8 | -2.3 | -1.3 | 0.7 | -3.7 | -6.0 | -1.7 | -1.1 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0946 | 3.145 | 2.6542 | 2.0216 | 1.993 | 2.7629 | 7.9491 | 9.3849 | 8.8108 | 8.5009 | 8.8798 | 9.4146 | 5.6355 | 6.1184 | 5.9117 | 5.128 | 5.408 | 6.2163 |
Actin | 0.0288 | 0.0003 | 0.001 | 0.0056 | 0.0176 | 0.0056 | 0.0054 | 0.0001 | 0 | 0 | 0.0014 | 0 | 0.0121 | 0.0001 | 0.0161 | 0 | 0.0005 | 0.0027 |
Bud | 0.0012 | 0.0005 | 0.0005 | 0.0009 | 0.0007 | 0.0006 | 0.0005 | 0.0008 | 0.0007 | 0.0002 | 0.0008 | 0.0004 | 0.0004 | 0.0006 | 0.0005 | 0.0008 | 0.0005 | 0.0006 |
Bud Neck | 0.0092 | 0.0015 | 0.0014 | 0.0006 | 0.0009 | 0.0025 | 0.0023 | 0.002 | 0.001 | 0.0012 | 0.002 | 0.0017 | 0.0011 | 0.0013 | 0.0025 | 0.0012 | 0.0015 | 0.0032 |
Bud Periphery | 0.0023 | 0.0008 | 0.0009 | 0.0009 | 0.0017 | 0.0017 | 0.0014 | 0.0015 | 0.002 | 0.0004 | 0.0021 | 0.0013 | 0.0007 | 0.0009 | 0.001 | 0.0015 | 0.0009 | 0.0019 |
Bud Site | 0.0078 | 0.0022 | 0.0019 | 0.0027 | 0.0013 | 0.0013 | 0.0025 | 0.0095 | 0.0015 | 0.0008 | 0.0041 | 0.001 | 0.0011 | 0.0074 | 0.0025 | 0.0023 | 0.0012 | 0.0018 |
Cell Periphery | 0.0023 | 0.0026 | 0.0024 | 0.0015 | 0.0014 | 0.0021 | 0.0031 | 0.0045 | 0.0023 | 0.0015 | 0.0026 | 0.0034 | 0.0016 | 0.0025 | 0.0024 | 0.0015 | 0.0015 | 0.0034 |
Cytoplasm | 0.0158 | 0.0229 | 0.015 | 0.0161 | 0.0016 | 0.0068 | 0.0075 | 0.0104 | 0.0153 | 0.0029 | 0.0039 | 0.0053 | 0.0205 | 0.0247 | 0.0143 | 0.005 | 0.0138 | 0.0064 |
Cytoplasmic Foci | 0.0166 | 0.0138 | 0.0072 | 0.022 | 0.0049 | 0.0048 | 0.0096 | 0.0078 | 0.0116 | 0.0047 | 0.0108 | 0.005 | 0.0132 | 0.0084 | 0.0121 | 0.0168 | 0.0119 | 0.0047 |
Eisosomes | 0.0009 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0062 | 0.0106 | 0.0065 | 0.0034 | 0.0019 | 0.0049 | 0.0064 | 0.0073 | 0.0058 | 0.0019 | 0.0013 | 0.0035 | 0.0115 | 0.0062 | 0.0053 | 0.0015 | 0.0089 | 0.0045 |
Endosome | 0.2682 | 0.1506 | 0.212 | 0.1863 | 0.2008 | 0.1228 | 0.2603 | 0.1075 | 0.1826 | 0.2161 | 0.2015 | 0.1403 | 0.2413 | 0.1505 | 0.2282 | 0.2876 | 0.2679 | 0.1524 |
Golgi | 0.0099 | 0.0054 | 0.0057 | 0.0035 | 0.0098 | 0.0033 | 0.0074 | 0.0025 | 0.0068 | 0.0031 | 0.0051 | 0.0035 | 0.0081 | 0.003 | 0.0087 | 0.0083 | 0.0092 | 0.0044 |
Lipid Particles | 0.0163 | 0.0041 | 0.0077 | 0.009 | 0.008 | 0.0047 | 0.0107 | 0.0022 | 0.0046 | 0.0037 | 0.0066 | 0.0066 | 0.0133 | 0.003 | 0.0089 | 0.006 | 0.0163 | 0.0053 |
Mitochondria | 0.0088 | 0.0037 | 0.0196 | 0.0033 | 0.0092 | 0.0023 | 0.0055 | 0.003 | 0.0173 | 0.0021 | 0.001 | 0.0021 | 0.0035 | 0.0018 | 0.0142 | 0.0441 | 0.0024 | 0.009 |
None | 0.0019 | 0.0006 | 0.0015 | 0.0114 | 0.0009 | 0.0003 | 0.002 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0017 | 0.0044 | 0.0097 | 0.0015 | 0.0002 | 0.0011 | 0.0006 |
Nuclear Periphery | 0.0154 | 0.0078 | 0.0114 | 0.0115 | 0.0067 | 0.0203 | 0.0055 | 0.0095 | 0.0085 | 0.0021 | 0.0019 | 0.0048 | 0.0151 | 0.0059 | 0.0086 | 0.0018 | 0.0127 | 0.0068 |
Nucleolus | 0.0033 | 0.0021 | 0.01 | 0.0043 | 0.0011 | 0.0038 | 0.0027 | 0.0023 | 0.0018 | 0.0019 | 0.0036 | 0.0022 | 0.0025 | 0.0026 | 0.0043 | 0.0012 | 0.0019 | 0.0036 |
Nucleus | 0.0096 | 0.0055 | 0.0067 | 0.0077 | 0.003 | 0.0142 | 0.0039 | 0.0052 | 0.0119 | 0.0028 | 0.004 | 0.004 | 0.0078 | 0.0066 | 0.0056 | 0.0023 | 0.0069 | 0.0109 |
Peroxisomes | 0.0067 | 0.0004 | 0.0004 | 0.0016 | 0.0023 | 0.0003 | 0.0018 | 0.0002 | 0.0017 | 0.0003 | 0.0136 | 0.0004 | 0.0009 | 0.0002 | 0.0008 | 0.0008 | 0.0013 | 0.0004 |
Punctate Nuclear | 0.003 | 0.0004 | 0.0011 | 0.019 | 0.0021 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.012 | 0.0003 | 0.0004 | 0.0001 | 0.0019 | 0.001 |
Vacuole | 0.4593 | 0.6649 | 0.5455 | 0.5445 | 0.6379 | 0.6709 | 0.5593 | 0.7217 | 0.5937 | 0.6269 | 0.6556 | 0.7072 | 0.5438 | 0.6831 | 0.5646 | 0.55 | 0.5481 | 0.6732 |
Vacuole Periphery | 0.1067 | 0.0992 | 0.1414 | 0.144 | 0.0859 | 0.1264 | 0.1016 | 0.1012 | 0.1302 | 0.1271 | 0.0778 | 0.1053 | 0.085 | 0.0813 | 0.0972 | 0.067 | 0.0896 | 0.1031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.765 | 21.6786 | 29.4918 | 35.2224 | 15.721 | 51.1675 | 41.4029 | 47.2586 | 43.5454 | 43.0478 |
Translational Efficiency | 0.9853 | 1.3446 | 1.0182 | 0.9068 | 2.1474 | 0.7898 | 1.0397 | 0.8092 | 0.9174 | 1.0298 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
139 | 1187 | 2290 | 1540 | 1918 | 1530 | 2208 | 2002 | 2057 | 2717 | 4498 | 3542 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 916.22 | 952.92 | 948.28 | 979.20 | 778.09 | 1003.77 | 978.42 | 998.92 | 787.42 | 981.55 | 963.08 | 990.35 |
Standard Deviation | 164.91 | 129.21 | 125.68 | 134.23 | 106.32 | 124.33 | 125.76 | 130.43 | 116.53 | 128.97 | 126.61 | 132.46 |
Intensity Change Log 2 | — | 0.056661 | 0.049619 | 0.095910 | — | 0.367420 | 0.330517 | 0.360432 | — | 0.207717 | 0.185434 | 0.223432 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000048 | 0.000038 | 0.000099 | 0.001085 | 0.000010 | 0.000028 | 0.000175 | 0.000986 | 0.000012 | 0.000033 | 0.000136 | 0.001029 |
Bud Neck | 0.008846 | 0.005955 | 0.021138 | 0.015043 | 0.012227 | 0.008088 | 0.019089 | 0.016638 | 0.011998 | 0.007156 | 0.020132 | 0.015945 |
Bud Site | 0.016368 | 0.005414 | 0.017137 | 0.025819 | 0.004760 | 0.004202 | 0.017413 | 0.037116 | 0.005545 | 0.004731 | 0.017272 | 0.032204 |
Cell Periphery | 0.003197 | 0.001102 | 0.000113 | 0.000242 | 0.000324 | 0.001295 | 0.000194 | 0.000517 | 0.000518 | 0.001210 | 0.000153 | 0.000397 |
Cytoplasm | 0.046414 | 0.006244 | 0.152989* | 0.119142* | 0.083082 | 0.005479 | 0.092401 | 0.146070* | 0.080604 | 0.005813 | 0.123248* | 0.134362* |
Cytoplasmic Foci | 0.005809 | 0.004473 | 0.035111 | 0.054614 | 0.018928 | 0.003242 | 0.030343 | 0.064167 | 0.018041 | 0.003780 | 0.032770 | 0.060013 |
Eisosomes | 0.000010 | 0.000008 | 0.000017 | 0.000029 | 0.000014 | 0.000011 | 0.000016 | 0.000039 | 0.000014 | 0.000010 | 0.000017 | 0.000035 |
Endoplasmic Reticulum | 0.002523 | 0.002643 | 0.005529 | 0.011858 | 0.001886 | 0.003306 | 0.006685 | 0.021198 | 0.001929 | 0.003016 | 0.006096 | 0.017137 |
Endosome | 0.047541 | 0.028465 | 0.107011 | 0.115221 | 0.034265 | 0.031602 | 0.127829 | 0.130976 | 0.035162 | 0.030232 | 0.117230 | 0.124126 |
Golgi | 0.002090 | 0.001594 | 0.000984 | 0.003489 | 0.000240 | 0.001419 | 0.001209 | 0.004664 | 0.000365 | 0.001495 | 0.001095 | 0.004153 |
Lipid Particles | 0.000876 | 0.000269 | 0.004851 | 0.006386 | 0.000794 | 0.000464 | 0.005555 | 0.011458 | 0.000800 | 0.000379 | 0.005197 | 0.009252 |
Mitochondria | 0.000259 | 0.000919 | 0.000478 | 0.000579 | 0.000187 | 0.002192 | 0.000381 | 0.000525 | 0.000192 | 0.001636 | 0.000431 | 0.000549 |
Mitotic Spindle | 0.000123 | 0.000316 | 0.003644 | 0.006779 | 0.000052 | 0.000103 | 0.000995 | 0.001559 | 0.000056 | 0.000196 | 0.002344 | 0.003829 |
None | 0.000631 | 0.000031 | 0.000866 | 0.000172 | 0.000747 | 0.000300 | 0.000135 | 0.000159 | 0.000739 | 0.000182 | 0.000507 | 0.000165 |
Nuclear Periphery | 0.000350 | 0.000129 | 0.001491 | 0.000447 | 0.000126 | 0.000174 | 0.000561 | 0.000190 | 0.000141 | 0.000154 | 0.001035 | 0.000302 |
Nuclear Periphery Foci | 0.000238 | 0.000151 | 0.004565 | 0.004264 | 0.000131 | 0.000112 | 0.002047 | 0.003154 | 0.000138 | 0.000129 | 0.003329 | 0.003636 |
Nucleolus | 0.000696 | 0.000544 | 0.001707 | 0.000736 | 0.000667 | 0.000462 | 0.000960 | 0.000579 | 0.000669 | 0.000498 | 0.001340 | 0.000647 |
Nucleus | 0.010831 | 0.002182 | 0.023488 | 0.005260 | 0.013396 | 0.002372 | 0.008858 | 0.004512 | 0.013223 | 0.002289 | 0.016306 | 0.004837 |
Peroxisomes | 0.000690 | 0.000446 | 0.002198 | 0.003779 | 0.000431 | 0.000500 | 0.002140 | 0.003982 | 0.000449 | 0.000476 | 0.002170 | 0.003894 |
Vacuole | 0.822363* | 0.900484* | 0.612134* | 0.618677* | 0.819612* | 0.909013* | 0.675298* | 0.543947* | 0.819798* | 0.905287* | 0.643140* | 0.576439* |
Vacuole Periphery | 0.030096 | 0.038594 | 0.004450 | 0.006381 | 0.008123 | 0.025636 | 0.007716 | 0.007563 | 0.009608 | 0.031297 | 0.006053 | 0.007049 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.54 | -4.23 | -1.75 | -1.77 | -1.61 | -1.24 | -5.23 | -6.50 | -6.35 | -5.12 | -1.77 | -7.91 | -3.97 | -3.88 | -3.36 |
Bud Neck | 0.90 | -4.06 | -1.86 | -6.68 | 4.62 | 2.97 | -5.05 | -2.84 | -6.12 | 2.33 | 4.14 | -7.16 | -2.99 | -8.94 | 4.94 |
Bud Site | 2.59 | -0.22 | -2.10 | -11.02 | -4.08 | 0.71 | -10.72 | -15.94 | -15.97 | -8.51 | 1.14 | -12.35 | -18.35 | -19.36 | -9.30 |
Cell Periphery | 3.05 | 4.58 | 4.41 | 6.67 | -3.88 | -7.05 | 3.11 | -2.71 | 5.66 | -5.84 | -6.23 | 6.03 | 2.08 | 8.49 | -7.12 |
Cytoplasm | 3.45 | -10.19 | -6.94 | -25.50 | 5.95 | 19.85 | -3.33 | -11.71 | -31.54 | -8.77 | 20.30 | -12.13 | -12.97 | -40.52 | -1.24 |
Cytoplasmic Foci | 0.75 | -15.61 | -17.72 | -19.99 | -5.88 | 10.81 | -7.04 | -17.67 | -26.00 | -12.56 | 10.95 | -10.88 | -20.84 | -32.75 | -13.14 |
Eisosomes | 0.88 | -2.88 | -3.71 | -5.13 | -2.41 | 1.86 | -2.04 | -7.30 | -8.01 | -6.47 | 3.46 | -3.64 | -7.70 | -9.41 | -6.43 |
Endoplasmic Reticulum | -0.18 | -3.19 | -4.69 | -4.90 | -3.30 | -5.30 | -6.11 | -10.44 | -9.55 | -7.22 | -5.38 | -8.35 | -11.58 | -10.62 | -7.98 |
Endosome | 1.80 | -4.26 | -5.22 | -18.14 | -2.34 | 1.08 | -20.95 | -22.53 | -22.74 | -0.83 | 2.30 | -24.15 | -25.82 | -29.04 | -2.63 |
Golgi | 0.40 | 1.14 | -0.80 | -1.71 | -3.38 | -2.40 | -4.67 | -7.53 | -4.06 | -5.58 | -2.92 | -4.06 | -8.20 | -4.17 | -6.49 |
Lipid Particles | 1.37 | -7.92 | -7.94 | -11.35 | -2.11 | 3.15 | -12.99 | -15.09 | -15.51 | -7.07 | 5.08 | -18.53 | -18.05 | -19.03 | -7.31 |
Mitochondria | -1.45 | -2.00 | -1.77 | 0.84 | -0.32 | -2.86 | -4.45 | -3.48 | 2.38 | -1.13 | -3.28 | -5.95 | -4.34 | 2.50 | -0.93 |
Mitotic Spindle | -1.12 | -5.45 | -4.67 | -4.53 | -0.51 | -1.11 | -4.83 | -2.89 | -2.78 | -1.03 | -1.92 | -6.42 | -5.52 | -5.31 | -0.50 |
None | 1.16 | -0.21 | 0.89 | -2.89 | 1.49 | 1.23 | 2.46 | 2.37 | 0.50 | -0.32 | 2.02 | 0.86 | 2.48 | 0.10 | 1.45 |
Nuclear Periphery | 0.79 | -4.19 | -0.57 | -6.18 | 6.13 | -1.02 | -7.77 | -3.84 | -0.44 | 6.60 | -0.41 | -10.00 | -6.20 | -4.41 | 8.20 |
Nuclear Periphery Foci | 1.01 | -9.18 | -5.68 | -5.91 | 1.18 | 0.64 | -8.06 | -8.89 | -8.93 | -1.48 | 0.51 | -12.16 | -9.72 | -9.76 | 0.61 |
Nucleolus | 0.55 | -4.91 | -0.44 | -1.52 | 7.20 | 1.73 | -4.26 | 0.29 | -0.87 | 3.01 | 1.52 | -8.22 | -0.28 | -1.61 | 7.14 |
Nucleus | 1.42 | -2.41 | 0.83 | -5.43 | 16.14 | 10.77 | 2.12 | 8.46 | -4.51 | 7.38 | 10.53 | -5.45 | 7.68 | -7.02 | 17.57 |
Peroxisomes | 0.92 | -5.00 | -6.52 | -7.69 | -3.55 | -0.29 | -5.85 | -10.08 | -8.23 | -3.87 | -0.18 | -9.65 | -12.69 | -11.24 | -5.35 |
Vacuole | -3.41 | 9.80 | 8.64 | 34.49* | -2.71 | -13.32 | 19.05 | 33.04 | 49.04* | 14.58 | -14.15 | 28.30 | 34.39 | 59.35* | 8.54 |
Vacuole Periphery | -1.46 | 4.84 | 4.47 | 14.46 | -2.85 | -11.05 | 1.09 | 1.30 | 11.76 | 0.15 | -15.24 | 5.90 | 4.16 | 18.54 | -2.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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