ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.28 0.19 0.34 0.4 0.38 0.43 0.49 0.49 0.51 0.59 0.62 0.21 0.55 0.72 0.19 0.16 0.11 0 0 0 0 0.07 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.15 0.15 0.4 0.2 0.15 0.11 0.18 0.18 0.13 0.13 0.08 0.05 0.08 0.18 0.12 0.41 0.4 0.33 0 0 0 0.05 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.06
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.78 0.76 0.67 0.79 0.66 0.74 0.69 0.68 0.65 0.63 0.54 0.6 0.71 0.37 0.28 0.57 0.61 0.65 0.88 0.87 0.91 0.53 0.66 0.66
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Bud 3 0 0 0 1 0 1 1 0 1 2 4 0 0 0 0 0 1 6 2 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 72 114 17 38 76 120 194 180 134 179 215 261 22 36 121 51 51 28 7 16 0 0 7 2
Endoplasmic Reticulum 3 5 1 0 1 0 3 0 0 0 0 0 5 1 1 8 7 14 0 0 0 0 2 3
Endosome 24 2 1 2 1 3 5 1 2 1 1 1 2 0 2 5 4 4 18 15 2 5 8 18
Golgi 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 8 3 1 2 0 1 1 2
Mitochondria 8 21 0 0 2 2 1 1 5 5 6 4 0 0 2 3 3 0 2 4 3 0 1 5
Nucleus 60 62 36 22 28 36 82 65 36 46 29 20 8 12 21 110 128 80 11 5 0 1 5 7
Nuclear Periphery 1 1 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 1 4 0 0 4 0 10
Nucleolus 2 0 0 1 0 0 0 0 0 1 1 1 0 0 0 1 0 0 1 0 0 1 1 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 2 2 0 1 1 1
Vac/Vac Membrane 319 306 61 88 125 233 310 251 178 219 195 250 75 24 48 153 196 160 414 374 118 22 72 116
Unique Cell Count 411 403 91 111 189 315 448 369 272 350 364 420 105 65 169 268 323 246 473 428 130 43 110 176
Labelled Cell Count 492 511 116 151 234 395 597 499 355 454 450 542 115 73 195 332 398 291 473 428 130 43 110 176


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 5.7 7.5 7.9 6.6 8.4 8.5 8.1 7.4 7.6 7.2 7.6 9.2 8.7 7.9 9.7 11.8 11.8 8.5 11.5 13.1
Std Deviation (1e-4) 1.2 1.1 1.3 1.5 1.9 1.8 1.9 1.6 1.9 1.8 1.7 1.9 2.2 2.4 2.1 3.8 3.9 3.5 2.1 3.5 3.4
Intensity Change (Log2) 0.08 -0.18 0.16 0.17 0.1 -0.03 0.02 -0.06 0.02 0.29 0.21 0.08 0.38 0.66 0.66 0.18 0.62 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 3.6 3.4 4.4 5.2 5.1 5.6 6.9 7.5 0.4 4.8 8.2 0.1 -0.7 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -3.1 -4.6 -6.2 -4.5 -4.5 -5.5 -5.8 -7.7 -9.6 -5.3 -2.8 -5.0 0.2 0 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 -0.1 1.3 0.4 0.2 -0.3 -0.8 -2.3 -1.3 0.7 -3.7 -6.0 -1.7 -1.1 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0946 3.145 2.6542 2.0216 1.993 2.7629 7.9491 9.3849 8.8108 8.5009 8.8798 9.4146 5.6355 6.1184 5.9117 5.128 5.408 6.2163
Actin 0.0288 0.0003 0.001 0.0056 0.0176 0.0056 0.0054 0.0001 0 0 0.0014 0 0.0121 0.0001 0.0161 0 0.0005 0.0027
Bud 0.0012 0.0005 0.0005 0.0009 0.0007 0.0006 0.0005 0.0008 0.0007 0.0002 0.0008 0.0004 0.0004 0.0006 0.0005 0.0008 0.0005 0.0006
Bud Neck 0.0092 0.0015 0.0014 0.0006 0.0009 0.0025 0.0023 0.002 0.001 0.0012 0.002 0.0017 0.0011 0.0013 0.0025 0.0012 0.0015 0.0032
Bud Periphery 0.0023 0.0008 0.0009 0.0009 0.0017 0.0017 0.0014 0.0015 0.002 0.0004 0.0021 0.0013 0.0007 0.0009 0.001 0.0015 0.0009 0.0019
Bud Site 0.0078 0.0022 0.0019 0.0027 0.0013 0.0013 0.0025 0.0095 0.0015 0.0008 0.0041 0.001 0.0011 0.0074 0.0025 0.0023 0.0012 0.0018
Cell Periphery 0.0023 0.0026 0.0024 0.0015 0.0014 0.0021 0.0031 0.0045 0.0023 0.0015 0.0026 0.0034 0.0016 0.0025 0.0024 0.0015 0.0015 0.0034
Cytoplasm 0.0158 0.0229 0.015 0.0161 0.0016 0.0068 0.0075 0.0104 0.0153 0.0029 0.0039 0.0053 0.0205 0.0247 0.0143 0.005 0.0138 0.0064
Cytoplasmic Foci 0.0166 0.0138 0.0072 0.022 0.0049 0.0048 0.0096 0.0078 0.0116 0.0047 0.0108 0.005 0.0132 0.0084 0.0121 0.0168 0.0119 0.0047
Eisosomes 0.0009 0 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0062 0.0106 0.0065 0.0034 0.0019 0.0049 0.0064 0.0073 0.0058 0.0019 0.0013 0.0035 0.0115 0.0062 0.0053 0.0015 0.0089 0.0045
Endosome 0.2682 0.1506 0.212 0.1863 0.2008 0.1228 0.2603 0.1075 0.1826 0.2161 0.2015 0.1403 0.2413 0.1505 0.2282 0.2876 0.2679 0.1524
Golgi 0.0099 0.0054 0.0057 0.0035 0.0098 0.0033 0.0074 0.0025 0.0068 0.0031 0.0051 0.0035 0.0081 0.003 0.0087 0.0083 0.0092 0.0044
Lipid Particles 0.0163 0.0041 0.0077 0.009 0.008 0.0047 0.0107 0.0022 0.0046 0.0037 0.0066 0.0066 0.0133 0.003 0.0089 0.006 0.0163 0.0053
Mitochondria 0.0088 0.0037 0.0196 0.0033 0.0092 0.0023 0.0055 0.003 0.0173 0.0021 0.001 0.0021 0.0035 0.0018 0.0142 0.0441 0.0024 0.009
None 0.0019 0.0006 0.0015 0.0114 0.0009 0.0003 0.002 0.0006 0.0003 0.0002 0.0002 0.0017 0.0044 0.0097 0.0015 0.0002 0.0011 0.0006
Nuclear Periphery 0.0154 0.0078 0.0114 0.0115 0.0067 0.0203 0.0055 0.0095 0.0085 0.0021 0.0019 0.0048 0.0151 0.0059 0.0086 0.0018 0.0127 0.0068
Nucleolus 0.0033 0.0021 0.01 0.0043 0.0011 0.0038 0.0027 0.0023 0.0018 0.0019 0.0036 0.0022 0.0025 0.0026 0.0043 0.0012 0.0019 0.0036
Nucleus 0.0096 0.0055 0.0067 0.0077 0.003 0.0142 0.0039 0.0052 0.0119 0.0028 0.004 0.004 0.0078 0.0066 0.0056 0.0023 0.0069 0.0109
Peroxisomes 0.0067 0.0004 0.0004 0.0016 0.0023 0.0003 0.0018 0.0002 0.0017 0.0003 0.0136 0.0004 0.0009 0.0002 0.0008 0.0008 0.0013 0.0004
Punctate Nuclear 0.003 0.0004 0.0011 0.019 0.0021 0.0002 0.0006 0.0002 0.0002 0.0001 0.0003 0.0002 0.012 0.0003 0.0004 0.0001 0.0019 0.001
Vacuole 0.4593 0.6649 0.5455 0.5445 0.6379 0.6709 0.5593 0.7217 0.5937 0.6269 0.6556 0.7072 0.5438 0.6831 0.5646 0.55 0.5481 0.6732
Vacuole Periphery 0.1067 0.0992 0.1414 0.144 0.0859 0.1264 0.1016 0.1012 0.1302 0.1271 0.0778 0.1053 0.085 0.0813 0.0972 0.067 0.0896 0.1031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.765 21.6786 29.4918 35.2224 15.721 51.1675 41.4029 47.2586 43.5454 43.0478
Translational Efficiency 0.9853 1.3446 1.0182 0.9068 2.1474 0.7898 1.0397 0.8092 0.9174 1.0298

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
139 1187 2290 1540 1918 1530 2208 2002 2057 2717 4498 3542

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 916.22 952.92 948.28 979.20 778.09 1003.77 978.42 998.92 787.42 981.55 963.08 990.35
Standard Deviation 164.91 129.21 125.68 134.23 106.32 124.33 125.76 130.43 116.53 128.97 126.61 132.46
Intensity Change Log 2 0.056661 0.049619 0.095910 0.367420 0.330517 0.360432 0.207717 0.185434 0.223432

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.000038 0.000099 0.001085 0.000010 0.000028 0.000175 0.000986 0.000012 0.000033 0.000136 0.001029
Bud Neck 0.008846 0.005955 0.021138 0.015043 0.012227 0.008088 0.019089 0.016638 0.011998 0.007156 0.020132 0.015945
Bud Site 0.016368 0.005414 0.017137 0.025819 0.004760 0.004202 0.017413 0.037116 0.005545 0.004731 0.017272 0.032204
Cell Periphery 0.003197 0.001102 0.000113 0.000242 0.000324 0.001295 0.000194 0.000517 0.000518 0.001210 0.000153 0.000397
Cytoplasm 0.046414 0.006244 0.152989* 0.119142* 0.083082 0.005479 0.092401 0.146070* 0.080604 0.005813 0.123248* 0.134362*
Cytoplasmic Foci 0.005809 0.004473 0.035111 0.054614 0.018928 0.003242 0.030343 0.064167 0.018041 0.003780 0.032770 0.060013
Eisosomes 0.000010 0.000008 0.000017 0.000029 0.000014 0.000011 0.000016 0.000039 0.000014 0.000010 0.000017 0.000035
Endoplasmic Reticulum 0.002523 0.002643 0.005529 0.011858 0.001886 0.003306 0.006685 0.021198 0.001929 0.003016 0.006096 0.017137
Endosome 0.047541 0.028465 0.107011 0.115221 0.034265 0.031602 0.127829 0.130976 0.035162 0.030232 0.117230 0.124126
Golgi 0.002090 0.001594 0.000984 0.003489 0.000240 0.001419 0.001209 0.004664 0.000365 0.001495 0.001095 0.004153
Lipid Particles 0.000876 0.000269 0.004851 0.006386 0.000794 0.000464 0.005555 0.011458 0.000800 0.000379 0.005197 0.009252
Mitochondria 0.000259 0.000919 0.000478 0.000579 0.000187 0.002192 0.000381 0.000525 0.000192 0.001636 0.000431 0.000549
Mitotic Spindle 0.000123 0.000316 0.003644 0.006779 0.000052 0.000103 0.000995 0.001559 0.000056 0.000196 0.002344 0.003829
None 0.000631 0.000031 0.000866 0.000172 0.000747 0.000300 0.000135 0.000159 0.000739 0.000182 0.000507 0.000165
Nuclear Periphery 0.000350 0.000129 0.001491 0.000447 0.000126 0.000174 0.000561 0.000190 0.000141 0.000154 0.001035 0.000302
Nuclear Periphery Foci 0.000238 0.000151 0.004565 0.004264 0.000131 0.000112 0.002047 0.003154 0.000138 0.000129 0.003329 0.003636
Nucleolus 0.000696 0.000544 0.001707 0.000736 0.000667 0.000462 0.000960 0.000579 0.000669 0.000498 0.001340 0.000647
Nucleus 0.010831 0.002182 0.023488 0.005260 0.013396 0.002372 0.008858 0.004512 0.013223 0.002289 0.016306 0.004837
Peroxisomes 0.000690 0.000446 0.002198 0.003779 0.000431 0.000500 0.002140 0.003982 0.000449 0.000476 0.002170 0.003894
Vacuole 0.822363* 0.900484* 0.612134* 0.618677* 0.819612* 0.909013* 0.675298* 0.543947* 0.819798* 0.905287* 0.643140* 0.576439*
Vacuole Periphery 0.030096 0.038594 0.004450 0.006381 0.008123 0.025636 0.007716 0.007563 0.009608 0.031297 0.006053 0.007049

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.54 -4.23 -1.75 -1.77 -1.61 -1.24 -5.23 -6.50 -6.35 -5.12 -1.77 -7.91 -3.97 -3.88 -3.36
Bud Neck 0.90 -4.06 -1.86 -6.68 4.62 2.97 -5.05 -2.84 -6.12 2.33 4.14 -7.16 -2.99 -8.94 4.94
Bud Site 2.59 -0.22 -2.10 -11.02 -4.08 0.71 -10.72 -15.94 -15.97 -8.51 1.14 -12.35 -18.35 -19.36 -9.30
Cell Periphery 3.05 4.58 4.41 6.67 -3.88 -7.05 3.11 -2.71 5.66 -5.84 -6.23 6.03 2.08 8.49 -7.12
Cytoplasm 3.45 -10.19 -6.94 -25.50 5.95 19.85 -3.33 -11.71 -31.54 -8.77 20.30 -12.13 -12.97 -40.52 -1.24
Cytoplasmic Foci 0.75 -15.61 -17.72 -19.99 -5.88 10.81 -7.04 -17.67 -26.00 -12.56 10.95 -10.88 -20.84 -32.75 -13.14
Eisosomes 0.88 -2.88 -3.71 -5.13 -2.41 1.86 -2.04 -7.30 -8.01 -6.47 3.46 -3.64 -7.70 -9.41 -6.43
Endoplasmic Reticulum -0.18 -3.19 -4.69 -4.90 -3.30 -5.30 -6.11 -10.44 -9.55 -7.22 -5.38 -8.35 -11.58 -10.62 -7.98
Endosome 1.80 -4.26 -5.22 -18.14 -2.34 1.08 -20.95 -22.53 -22.74 -0.83 2.30 -24.15 -25.82 -29.04 -2.63
Golgi 0.40 1.14 -0.80 -1.71 -3.38 -2.40 -4.67 -7.53 -4.06 -5.58 -2.92 -4.06 -8.20 -4.17 -6.49
Lipid Particles 1.37 -7.92 -7.94 -11.35 -2.11 3.15 -12.99 -15.09 -15.51 -7.07 5.08 -18.53 -18.05 -19.03 -7.31
Mitochondria -1.45 -2.00 -1.77 0.84 -0.32 -2.86 -4.45 -3.48 2.38 -1.13 -3.28 -5.95 -4.34 2.50 -0.93
Mitotic Spindle -1.12 -5.45 -4.67 -4.53 -0.51 -1.11 -4.83 -2.89 -2.78 -1.03 -1.92 -6.42 -5.52 -5.31 -0.50
None 1.16 -0.21 0.89 -2.89 1.49 1.23 2.46 2.37 0.50 -0.32 2.02 0.86 2.48 0.10 1.45
Nuclear Periphery 0.79 -4.19 -0.57 -6.18 6.13 -1.02 -7.77 -3.84 -0.44 6.60 -0.41 -10.00 -6.20 -4.41 8.20
Nuclear Periphery Foci 1.01 -9.18 -5.68 -5.91 1.18 0.64 -8.06 -8.89 -8.93 -1.48 0.51 -12.16 -9.72 -9.76 0.61
Nucleolus 0.55 -4.91 -0.44 -1.52 7.20 1.73 -4.26 0.29 -0.87 3.01 1.52 -8.22 -0.28 -1.61 7.14
Nucleus 1.42 -2.41 0.83 -5.43 16.14 10.77 2.12 8.46 -4.51 7.38 10.53 -5.45 7.68 -7.02 17.57
Peroxisomes 0.92 -5.00 -6.52 -7.69 -3.55 -0.29 -5.85 -10.08 -8.23 -3.87 -0.18 -9.65 -12.69 -11.24 -5.35
Vacuole -3.41 9.80 8.64 34.49* -2.71 -13.32 19.05 33.04 49.04* 14.58 -14.15 28.30 34.39 59.35* 8.54
Vacuole Periphery -1.46 4.84 4.47 14.46 -2.85 -11.05 1.09 1.30 11.76 0.15 -15.24 5.90 4.16 18.54 -2.14