Standard name
Description Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.19 0.06 0.08 0 0 0.06 0 0 0.07 0.05 0.16 0.17 0.08 0.11 0.06 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.05 0.06 0.09 0.1 0.12 0.08 0.05 0 0 0.05 0.05 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.1 0.12 0.08 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.12 0.1 0.12 0.2 0.11 0.09 0.06 0.16 0 0 0 0.09 0.14 0.1 0.08 0.12 0.1 0.18 0.12 0.11
Golgi 0.81 0.78 0.75 0.72 0.7 0.39 0.4 0.24 0.14 0.12 0.12 0.88 0.83 0.83 0.72 0.61 0.74 0.85 0.75 0.79 0.69 0.8 0.8
Mitochondria 0.34 0.41 0.47 0.37 0.19 0.54 0.32 0.6 0.65 0.73 0.67 0.19 0.11 0.05 0.06 0.13 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.16 0.09 0.05 0.16 0.1 0.13 0.08 0.06 0.06 0.06 0.2 0.2 0.25 0.12 0.16 0.15 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 48 79 21 26 17 12 33 18 13 39 28 43 36 18 29 7 9 9 18 15 7 11 17
Bud 5 1 3 4 5 11 20 26 24 39 40 0 0 0 5 0 1 1 1 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 0 1 3 0 0 0 0 0 0
Cell Periphery 1 2 2 0 1 5 3 5 4 5 16 2 3 0 2 2 0 0 0 0 0 0 0
Cytoplasm 11 14 19 19 22 23 45 43 52 47 27 10 7 13 12 7 13 0 0 1 1 0 1
Endoplasmic Reticulum 6 13 7 10 6 12 12 15 18 23 16 28 26 18 7 0 2 0 1 2 0 2 2
Endosome 12 4 13 38 40 50 103 46 37 35 90 4 0 2 24 16 13 28 53 34 41 61 50
Golgi 278 328 249 222 284 161 209 100 59 65 68 241 176 197 184 68 93 299 348 279 158 406 380
Mitochondria 117 173 156 115 78 226 168 246 274 404 375 51 23 13 16 14 10 2 12 5 6 7 7
Nucleus 0 0 0 0 1 1 2 2 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 2 0 1 3 3 0 1 0 0 1 0 1 0 0 1 0 0 0
Nucleolus 10 12 12 12 7 21 29 14 10 23 16 2 2 1 5 8 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 3 3 2 1 6 0 0 0 0 0 0 3 2 4 4 8 4
SpindlePole 1 0 0 2 5 3 11 6 4 6 13 0 0 1 2 0 0 0 1 0 0 1 0
Vac/Vac Membrane 66 67 31 17 63 43 65 32 25 35 36 54 42 59 30 18 19 7 23 4 3 5 6
Unique Cell Count 345 422 330 310 405 417 518 412 424 554 562 274 211 238 255 111 125 354 467 352 229 509 473
Labelled Cell Count 555 693 513 465 530 571 703 558 525 727 734 437 315 322 317 141 162 354 467 352 229 509 473


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.1 18.7 15.2 14.5 13.2 11.2 10.3 9.3 8.6 8.8 8.7 18.1 21.1 20.9 12.0 13.0 15.8 15.4 17.1 17.6
Std Deviation (1e-4) 3.1 3.1 2.6 2.5 2.2 1.9 1.6 1.6 1.5 1.7 1.5 3.4 4.1 4.2 2.4 3.5 3.4 2.8 3.1 3.2
Intensity Change (Log2) -0.06 -0.2 -0.44 -0.57 -0.7 -0.82 -0.79 -0.8 0.25 0.48 0.46 -0.34 -0.23 0.05 0.02 0.17 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -1.3 -2.3 0 -1.2 -2.2 0.4 -0.9 3.7 4.0 0.6 2.1 0 0.3
Bud 0 0 1.7 2.6 3.8 3.5 4.1 4.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.3 0 0 0 0 0 0
Cytoplasm 0.2 -0.2 -0.1 1.6 2.3 3.0 1.5 -0.6 -1.2 -1.3 -0.2 -0.6 0.2 1.7
Endoplasmic Reticulum 0.9 -0.7 0.7 0.2 1.2 1.6 1.6 0.7 4.2 4.8 3.1 0.5 0 0
Endosome 3.9 3.1 3.9 6.6 3.6 2.6 1.5 5.4 -1.8 -2.9 -2.3 2.7 3.9 2.7
Golgi -1.1 -1.6 -10.0 -10.0 -13.9 -17.1 -19.1 -19.1 3.9 2.2 2.1 -0.9 -2.9 -0.2
Mitochondria -2.6 -8.1 1.9 -4.3 3.4 4.8 7.7 5.7 -7.4 -8.8 -10.8 -10.8 -6.5 -7.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 -1.6 0.9 1.3 -0.2 -1.0 0.4 -0.7 -2.4 -1.9 -2.5 -1.2 1.6 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 2.5 0.4 1.4 -0.8 -1.8 -1.7 -1.6 3.6 3.5 5.0 0.9 2.0 1.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Akr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available