Standard name
Description Mitochondrial tryptophanyl-tRNA synthetase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0 0 0 0 0 0 0 0 0 0.12 0.16 0.21 0.17 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 – 0.1 0 0.07 0 0 0 0 0 0 0 0.16 0.28 0.26 0.22 0.28 0.32 0.07 0.15 0.08 0.05 0.09 0.1
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.95 – 0.74 0.87 0.86 0.93 0.92 0.89 0.94 0.97 0.96 0.97 0.48 0.22 0.23 0.15 0.07 0.06 0.8 0.59 0.69 0.7 0.61 0.58
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0.08 0.05 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0.19 0.14 0.11 0 0 0.05 0 0 0 0 0.12 0.17 0.18 0.28 0.34 0.28 0 0 0.08 0 0 0
SpindlePole 0.05 – 0.12 0.08 0.06 0 0 0.07 0 0 0 0 0.17 0.27 0.22 0.28 0.25 0.3 0 0 0 0.07 0.07 0.07
Vac/Vac Membrane 0.11 – 0.06 0 0.1 0.07 0 0 0.06 0 0 0.07 0.1 0.15 0.13 0.15 0.1 0.16 0 0.08 0 0 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 1 0 0 1 0 1 1 0 0 18 1 9 0 1 2 0 0 4 0 7 8
Bud 7 – 9 13 16 12 37 20 15 24 17 24 15 13 15 7 7 8 0 1 1 1 4 5
Bud Neck 0 – 2 2 0 0 2 0 0 1 1 0 0 3 1 0 2 0 1 0 0 0 4 5
Bud Site 2 – 4 3 5 8 13 16 9 10 5 11 4 0 3 4 2 4 – – – – – –
Cell Periphery 0 – 7 7 4 7 6 3 5 6 1 5 9 13 10 6 9 6 0 0 1 0 0 0
Cytoplasm 4 – 9 8 11 8 14 5 7 3 3 3 57 96 136 36 15 18 0 0 0 2 10 11
Endoplasmic Reticulum 1 – 0 1 2 2 0 0 1 2 3 0 3 4 3 13 11 5 0 0 1 0 1 2
Endosome 0 – 24 13 37 4 5 31 2 2 0 0 74 165 165 45 49 47 12 4 20 4 41 48
Golgi 0 – 0 0 0 0 0 0 0 0 0 1 7 5 3 4 7 1 0 0 3 0 6 6
Mitochondria 162 – 170 294 430 478 710 681 547 670 483 574 225 126 149 31 13 9 139 19 170 65 274 269
Nucleus 1 – 5 1 4 13 27 14 6 9 3 8 2 2 3 3 2 4 0 1 0 0 4 3
Nuclear Periphery 1 – 1 4 1 20 27 23 49 37 43 34 0 0 0 0 1 0 1 0 2 0 1 1
Nucleolus 4 – 0 3 9 11 52 44 28 20 16 21 2 2 2 4 2 4 1 0 0 0 4 3
Peroxisomes 2 – 44 48 53 12 32 42 3 2 2 7 58 102 115 58 61 42 3 0 18 3 15 19
SpindlePole 8 – 28 27 28 18 31 55 14 12 7 23 81 160 141 58 44 45 6 0 10 6 31 30
Vac/Vac Membrane 19 – 15 13 50 36 28 29 36 14 16 40 45 87 85 31 18 23 4 2 5 3 38 40
Unique Cell Count 170 231 339 500 514 772 767 583 692 503 593 469 584 640 208 178 148 176 33 247 93 454 465
Labelled Cell Count 211 318 438 650 629 985 963 723 813 600 751 600 779 840 300 244 218 176 33 247 93 454 465


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 – 4.7 4.5 4.7 4.3 4.2 4.4 4.3 4.2 3.9 4.2 5.9 5.8 5.7 6.6 6.8 6.3 5.5 5.9 6.3
Std Deviation (1e-4) 0.5 – 1.4 1.3 1.2 1.1 1.2 1.1 1.0 0.9 0.8 1.0 1.0 1.0 1.0 1.9 1.7 1.4 1.7 1.2 1.5
Intensity Change (Log2) – – – -0.06 0.03 -0.11 -0.15 -0.07 -0.12 -0.15 -0.24 -0.15 0.34 0.33 0.3 0.51 0.54 0.43 0.23 0.35 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -0.5 -1.3 -1.2 -0.3 0 0.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 -0.9 -0.7 0 -0.1 1.0 0.5
Cytoplasm -1.1 -1.3 -2.0 -1.9 0 0 0 0 0 3.5 4.8 6.1 4.6 1.9 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.9 5.4 4.9 3.2 4.5 5.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.4 -13.9 -13.6 -12.3 -13.4 -12.8
Nucleus 0 0 0.3 1.0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -2.4 -0.5 -0.4 2.2 3.5 2.1
SpindlePole 0 0 0 0 0 0 0 0 0 1.8 4.7 3.3 4.2 3.3 4.4
Vacuole -1.4 1.5 0.3 -1.9 -1.8 -0.2 -3.4 -2.1 0.1 1.4 3.3 2.8 2.9 1.3 2.9