Standard name
Human Ortholog
Description Mitochondrial tryptophanyl-tRNA synthetase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0 0 0 0 0 0 0 0 0 0.12 0.16 0.21 0.17 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 – 0.1 0 0.07 0 0 0 0 0 0 0 0.16 0.28 0.26 0.22 0.28 0.32 0.07 0.15 0.08 0.05 0.09 0.1
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.95 – 0.74 0.87 0.86 0.93 0.92 0.89 0.94 0.97 0.96 0.97 0.48 0.22 0.23 0.15 0.07 0.06 0.8 0.59 0.69 0.7 0.61 0.58
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0.08 0.05 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0.19 0.14 0.11 0 0 0.05 0 0 0 0 0.12 0.17 0.18 0.28 0.34 0.28 0 0 0.08 0 0 0
SpindlePole 0.05 – 0.12 0.08 0.06 0 0 0.07 0 0 0 0 0.17 0.27 0.22 0.28 0.25 0.3 0 0 0 0.07 0.07 0.07
Vac/Vac Membrane 0.11 – 0.06 0 0.1 0.07 0 0 0.06 0 0 0.07 0.1 0.15 0.13 0.15 0.1 0.16 0 0.08 0 0 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 1 0 0 1 0 1 1 0 0 18 1 9 0 1 2 0 0 4 0 7 8
Bud 7 – 9 13 16 12 37 20 15 24 17 24 15 13 15 7 7 8 0 1 1 1 4 5
Bud Neck 0 – 2 2 0 0 2 0 0 1 1 0 0 3 1 0 2 0 1 0 0 0 4 5
Bud Site 2 – 4 3 5 8 13 16 9 10 5 11 4 0 3 4 2 4 – – – – – –
Cell Periphery 0 – 7 7 4 7 6 3 5 6 1 5 9 13 10 6 9 6 0 0 1 0 0 0
Cytoplasm 4 – 9 8 11 8 14 5 7 3 3 3 57 96 136 36 15 18 0 0 0 2 10 11
Endoplasmic Reticulum 1 – 0 1 2 2 0 0 1 2 3 0 3 4 3 13 11 5 0 0 1 0 1 2
Endosome 0 – 24 13 37 4 5 31 2 2 0 0 74 165 165 45 49 47 12 4 20 4 41 48
Golgi 0 – 0 0 0 0 0 0 0 0 0 1 7 5 3 4 7 1 0 0 3 0 6 6
Mitochondria 162 – 170 294 430 478 710 681 547 670 483 574 225 126 149 31 13 9 139 19 170 65 274 269
Nucleus 1 – 5 1 4 13 27 14 6 9 3 8 2 2 3 3 2 4 0 1 0 0 4 3
Nuclear Periphery 1 – 1 4 1 20 27 23 49 37 43 34 0 0 0 0 1 0 1 0 2 0 1 1
Nucleolus 4 – 0 3 9 11 52 44 28 20 16 21 2 2 2 4 2 4 1 0 0 0 4 3
Peroxisomes 2 – 44 48 53 12 32 42 3 2 2 7 58 102 115 58 61 42 3 0 18 3 15 19
SpindlePole 8 – 28 27 28 18 31 55 14 12 7 23 81 160 141 58 44 45 6 0 10 6 31 30
Vac/Vac Membrane 19 – 15 13 50 36 28 29 36 14 16 40 45 87 85 31 18 23 4 2 5 3 38 40
Unique Cell Count 170 231 339 500 514 772 767 583 692 503 593 469 584 640 208 178 148 176 33 247 93 454 465
Labelled Cell Count 211 318 438 650 629 985 963 723 813 600 751 600 779 840 300 244 218 176 33 247 93 454 465


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 – 4.7 4.5 4.7 4.3 4.2 4.4 4.3 4.2 3.9 4.2 5.9 5.8 5.7 6.6 6.8 6.3 5.5 5.9 6.3
Std Deviation (1e-4) 0.5 – 1.4 1.3 1.2 1.1 1.2 1.1 1.0 0.9 0.8 1.0 1.0 1.0 1.0 1.9 1.7 1.4 1.7 1.2 1.5
Intensity Change (Log2) – – – -0.06 0.03 -0.11 -0.15 -0.07 -0.12 -0.15 -0.24 -0.15 0.34 0.33 0.3 0.51 0.54 0.43 0.23 0.35 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 3.0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -0.5 -1.3 -1.2 -0.3 0 0.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 -0.9 -0.7 0 -0.1 1.0 0.5
Cytoplasm -1.1 -1.3 -2.0 -1.9 0 0 0 0 0 3.5 4.8 6.1 4.6 1.9 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.9 5.4 4.9 3.2 4.5 5.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.4 -13.9 -13.6 -12.3 -13.4 -12.8
Nucleus 0 0 0.3 1.0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -2.4 -0.5 -0.4 2.2 3.5 2.1
SpindlePole 0 0 0 0 0 0 0 0 0 1.8 4.7 3.3 4.2 3.3 4.4
Vacuole -1.4 1.5 0.3 -1.9 -1.8 -0.2 -3.4 -2.1 0.1 1.4 3.3 2.8 2.9 1.3 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7404 1.727 1.4403 1.086 1.1247 1.418 0.5103 0.8762 0.6369 0.6841 0.5863 0.5063 -0.1638 0.403 0.0843 0.0302 -0.6439 -0.0352
Actin 0.0373 0.0059 0.0233 0.0003 0.004 0.0134 0.0546 0.0204 0.033 0.0941 0.0016 0.017 0.0259 0.0223 0.0106 0.0329 0.0008 0.0165
Bud 0.0006 0.001 0.0005 0.0004 0.0043 0.0002 0.0008 0.0038 0.0022 0.0179 0.0002 0.0013 0.0006 0.0051 0.0005 0.0006 0.0006 0.0009
Bud Neck 0.0007 0.0005 0.0006 0.0002 0.0022 0.0005 0.0011 0.001 0.0029 0.0006 0.0001 0.0081 0.0011 0.0005 0.0012 0.0008 0.0002 0.001
Bud Periphery 0.001 0.0013 0.0008 0.0007 0.0054 0.0006 0.0012 0.0071 0.0036 0.0102 0.0003 0.0058 0.0012 0.0062 0.0009 0.001 0.0022 0.0018
Bud Site 0.0019 0.008 0.0071 0.0006 0.0071 0.0004 0.0051 0.0178 0.0101 0.0087 0.0004 0.0007 0.0026 0.0101 0.0023 0.0026 0.0008 0.0008
Cell Periphery 0.0003 0.0003 0.0004 0.0003 0.0004 0.0004 0.0003 0.0011 0.0006 0.0012 0.0001 0.0002 0.0007 0.001 0.0004 0.0001 0.0002 0.0008
Cytoplasm 0.0185 0.0029 0.0052 0.0032 0.0088 0.0057 0.0105 0.0187 0.0045 0.0036 0.0033 0.0041 0.0056 0.0057 0.002 0.0005 0.0005 0.0025
Cytoplasmic Foci 0.0599 0.04 0.0204 0.0082 0.0785 0.012 0.0412 0.0422 0.0304 0.0169 0.0108 0.0296 0.0246 0.0283 0.028 0.0238 0.015 0.0134
Eisosomes 0.0005 0.0003 0.0003 0.0001 0.0002 0.0016 0.0007 0.0006 0.0005 0.0009 0.0002 0.0003 0.0004 0.0006 0.0002 0.0004 0.0001 0.0003
Endoplasmic Reticulum 0.0033 0.001 0.0009 0.0002 0.0001 0.0008 0.0021 0.0026 0.0006 0.0019 0.001 0.0006 0.0023 0.0027 0.0005 0.0013 0.0003 0.0018
Endosome 0.0727 0.0704 0.0356 0.0329 0.0245 0.0313 0.0744 0.0987 0.0547 0.029 0.0494 0.0383 0.1002 0.0909 0.0737 0.0373 0.0511 0.0473
Golgi 0.0621 0.0674 0.032 0.0181 0.0176 0.06 0.0707 0.0913 0.0571 0.0475 0.0565 0.0305 0.055 0.08 0.0774 0.0469 0.0354 0.0614
Lipid Particles 0.0472 0.0209 0.0127 0.0096 0.0099 0.0157 0.0336 0.0151 0.0134 0.0357 0.0222 0.0224 0.0479 0.0169 0.034 0.044 0.0088 0.0159
Mitochondria 0.5627 0.7094 0.8118 0.9136 0.7913 0.7935 0.6179 0.622 0.7574 0.6882 0.8069 0.7968 0.6483 0.6883 0.7167 0.7629 0.8663 0.8029
None 0.0198 0.0115 0.0009 0.0004 0.0029 0.0081 0.0141 0.0068 0.002 0.0061 0.0136 0.0003 0.0052 0.0035 0.0004 0.0011 0.0001 0.0006
Nuclear Periphery 0.0145 0.0004 0.0067 0 0.0001 0.0023 0.0034 0.0012 0.0003 0.0004 0.0047 0.0005 0.0045 0.001 0.0002 0.0052 0.0001 0.0008
Nucleolus 0.001 0.0011 0.0002 0.0001 0.0007 0.0002 0.0005 0.0007 0.0003 0.0019 0.002 0.0001 0.001 0.0004 0.0005 0.0004 0.0005 0.0003
Nucleus 0.0113 0.0004 0.0014 0.0001 0.0003 0.0002 0.001 0.0009 0.0004 0.002 0.0038 0.0003 0.0009 0.0004 0.0002 0.0005 0.0002 0.0003
Peroxisomes 0.0685 0.0459 0.0317 0.0058 0.0381 0.0441 0.0558 0.0297 0.0189 0.0281 0.0073 0.0387 0.0458 0.0219 0.0404 0.032 0.0115 0.0164
Punctate Nuclear 0.0101 0.0008 0.0035 0 0.0005 0.0045 0.0023 0.0036 0.0009 0.0006 0.0095 0.0002 0.003 0.0006 0.0001 0.0026 0.0001 0.0013
Vacuole 0.0032 0.0068 0.0019 0.0027 0.0016 0.0013 0.0041 0.0104 0.0038 0.0021 0.0031 0.0018 0.0137 0.0061 0.0049 0.0011 0.0026 0.0055
Vacuole Periphery 0.0027 0.0037 0.0023 0.0025 0.0016 0.0036 0.0045 0.0044 0.0022 0.0024 0.0028 0.0025 0.0096 0.0073 0.0048 0.002 0.0028 0.0073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.3901 24.2268 13.483 12.0001 9.4119 10.641 14.8724 20.6662 17.8765 17.9075
Translational Efficiency 0.6824 0.4452 0.8302 1.0943 1.0609 1.0033 1.0833 0.846 0.9161 0.6136

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial tryptophanyl-tRNA synthetase
Localization
Cell Percentages mitochondrion (88%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Msw1

Msw1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msw1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available