Standard name | |
---|---|
Description | Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 6 | 1 | 1 | 1 | 0 | 2 | 2 | 2 | 0 | 4 | 0 | 5 | 6 | 2 | 3 | 2 | 2 | 2 | 1 | 7 | 1 | 2 | 3 |
Bud | 2 | 1 | 7 | 2 | 7 | 6 | 16 | 34 | 18 | 23 | 20 | 22 | 0 | 1 | 0 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 153 | 57 | 62 | 44 | 130 | 202 | 287 | 308 | 192 | 262 | 188 | 247 | 2 | 13 | 13 | 175 | 150 | 72 | 14 | 8 | 1 | 4 | 7 | 11 |
Endoplasmic Reticulum | 14 | 12 | 6 | 3 | 2 | 5 | 2 | 3 | 1 | 4 | 1 | 5 | 8 | 3 | 11 | 23 | 34 | 19 | 3 | 0 | 1 | 4 | 5 | 3 |
Endosome | 87 | 1 | 100 | 88 | 129 | 56 | 135 | 169 | 49 | 14 | 20 | 42 | 7 | 11 | 17 | 93 | 130 | 71 | 153 | 38 | 153 | 55 | 46 | 41 |
Golgi | 2 | 0 | 13 | 5 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 33 | 61 | 5 | 16 | 22 | 59 | 12 | 79 | 24 | 13 | 16 |
Mitochondria | 40 | 75 | 100 | 63 | 62 | 148 | 202 | 248 | 180 | 290 | 162 | 195 | 18 | 19 | 5 | 42 | 49 | 25 | 81 | 41 | 32 | 40 | 39 | 37 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 6 | 2 | 2 | 1 | 2 | 0 | 1 | 0 | 11 | 16 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 3 | 0 | 0 | 0 | 0 | 2 | 7 | 1 | 4 | 2 | 2 | 2 | 0 | 1 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 1 | 4 | 1 | 1 | 2 | 6 | 21 | 3 | 3 | 0 | 1 | 3 | 4 | 0 | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 8 | 4 | 7 | 2 | 8 | 9 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 12 | 1 | 1 | 0 |
SpindlePole | 12 | 1 | 13 | 8 | 3 | 8 | 16 | 37 | 8 | 8 | 6 | 14 | 2 | 2 | 1 | 24 | 7 | 8 | 4 | 0 | 2 | 2 | 1 | 2 |
Vac/Vac Membrane | 145 | 37 | 76 | 44 | 153 | 105 | 172 | 144 | 64 | 112 | 65 | 84 | 6 | 40 | 109 | 386 | 488 | 343 | 180 | 70 | 63 | 77 | 89 | 99 |
Unique Cell Count | 395 | 146 | 302 | 203 | 383 | 455 | 655 | 781 | 418 | 572 | 383 | 491 | 37 | 92 | 178 | 587 | 721 | 465 | 509 | 180 | 360 | 216 | 211 | 221 |
Labelled Cell Count | 468 | 194 | 391 | 264 | 496 | 537 | 851 | 991 | 524 | 725 | 472 | 618 | 58 | 133 | 220 | 773 | 902 | 580 | 509 | 180 | 360 | 216 | 211 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 4.9 | 5.6 | 5.6 | 5.6 | 4.8 | 4.7 | 4.9 | 4.9 | 4.3 | 4.7 | 4.5 | 7.7 | 9.3 | 10.7 | 6.4 | 7.6 | 9.3 | 6.6 | 6.7 | 7.3 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.4 | 1.3 | 1.8 | 1.2 | 1.2 | 1.2 | 1.4 | 1.1 | 1.4 | 1.0 | 1.6 | 1.8 | 1.5 | 1.4 | 1.7 | 2.2 | 1.3 | 1.6 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.01 | -0.23 | -0.26 | -0.19 | -0.21 | -0.4 | -0.27 | -0.32 | 0.45 | 0.73 | 0.93 | 0.18 | 0.44 | 0.72 | 0.24 | 0.26 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 11.5 | 12.5 | 13.2 |