Standard name
Human Ortholog
Description Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.07 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.39 0.39 0.21 0.22 0.34 0.44 0.44 0.39 0.46 0.46 0.49 0.5 0.05 0.14 0.07 0.3 0.21 0.15 0 0.05 0 0 0 0.05
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0.22 0 0.06 0 0.05 0 0 0 0 0 0 0
Endosome 0.22 0 0.33 0.43 0.34 0.12 0.21 0.22 0.12 0 0.05 0.09 0.19 0.12 0.1 0.16 0.18 0.15 0.3 0.22 0.43 0.26 0.22 0.19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.36 0.34 0 0 0.05 0.12 0.07 0.22 0.11 0.07 0.07
Mitochondria 0.1 0.51 0.33 0.31 0.16 0.33 0.31 0.32 0.43 0.51 0.42 0.4 0.49 0.21 0 0.07 0.07 0.05 0.16 0.23 0.09 0.19 0.19 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.25 0.25 0.22 0.4 0.23 0.26 0.18 0.15 0.2 0.17 0.17 0.16 0.43 0.61 0.66 0.68 0.74 0.35 0.39 0.18 0.36 0.43 0.45
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 6 1 1 1 0 2 2 2 0 4 0 5 6 2 3 2 2 2 1 7 1 2 3
Bud 2 1 7 2 7 6 16 34 18 23 20 22 0 1 0 2 3 2 1 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 0 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 1 2 1 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 153 57 62 44 130 202 287 308 192 262 188 247 2 13 13 175 150 72 14 8 1 4 7 11
Endoplasmic Reticulum 14 12 6 3 2 5 2 3 1 4 1 5 8 3 11 23 34 19 3 0 1 4 5 3
Endosome 87 1 100 88 129 56 135 169 49 14 20 42 7 11 17 93 130 71 153 38 153 55 46 41
Golgi 2 0 13 5 1 1 0 0 1 0 0 0 5 33 61 5 16 22 59 12 79 24 13 16
Mitochondria 40 75 100 63 62 148 202 248 180 290 162 195 18 19 5 42 49 25 81 41 32 40 39 37
Nucleus 1 0 1 0 0 1 1 6 2 2 1 2 0 1 0 11 16 9 0 0 0 0 0 0
Nuclear Periphery 0 3 0 0 0 0 2 7 1 4 2 2 2 0 1 3 1 2 1 0 0 0 0 1
Nucleolus 1 1 4 1 1 2 6 21 3 3 0 1 3 4 0 4 3 4 0 0 0 0 0 0
Peroxisomes 1 0 8 4 7 2 8 9 2 2 2 2 0 0 0 1 2 1 2 1 12 1 1 0
SpindlePole 12 1 13 8 3 8 16 37 8 8 6 14 2 2 1 24 7 8 4 0 2 2 1 2
Vac/Vac Membrane 145 37 76 44 153 105 172 144 64 112 65 84 6 40 109 386 488 343 180 70 63 77 89 99
Unique Cell Count 395 146 302 203 383 455 655 781 418 572 383 491 37 92 178 587 721 465 509 180 360 216 211 221
Labelled Cell Count 468 194 391 264 496 537 851 991 524 725 472 618 58 133 220 773 902 580 509 180 360 216 211 221


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.9 5.6 5.6 5.6 4.8 4.7 4.9 4.9 4.3 4.7 4.5 7.7 9.3 10.7 6.4 7.6 9.3 6.6 6.7 7.3
Std Deviation (1e-4) 0.8 0.9 1.4 1.3 1.8 1.2 1.2 1.2 1.4 1.1 1.4 1.0 1.6 1.8 1.5 1.4 1.7 2.2 1.3 1.6 2.2
Intensity Change (Log2) – – – -0.01 0.01 -0.23 -0.26 -0.19 -0.21 -0.4 -0.27 -0.32 0.45 0.73 0.93 0.18 0.44 0.72 0.24 0.26 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 11.5 12.5 13.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0469 0.0014 0.0023 0.002 0.0091 0.0014 0.0006 0.0002 0.0024 0.0011 0.0305 0.0011 – – – – – –
Bud 0.0013 0.0012 0.0004 0.0006 0.0006 0.0003 0.0002 0.0006 0.0003 0.0002 0.0005 0.0004 – – – – – –
Bud Neck 0.0019 0.0018 0.0017 0.0009 0.001 0.0009 0.0004 0.0004 0.0032 0.0003 0.0004 0.0005 – – – – – –
Bud Periphery 0.0029 0.001 0.0004 0.0005 0.001 0.0005 0.0003 0.0008 0.0005 0.0002 0.0006 0.0008 – – – – – –
Bud Site 0.004 0.0138 0.0081 0.0015 0.0042 0.0003 0.001 0.0021 0.0025 0.0004 0.0022 0.0003 – – – – – –
Cell Periphery 0.0006 0.0007 0.0002 0.0001 0.0004 0.0002 0.0002 0.0007 0.0003 0.0001 0.0001 0.0002 – – – – – –
Cytoplasm 0.0169 0.0414 0.016 0.0144 0.0109 0.0156 0.0118 0.0131 0.0351 0.0075 0.0015 0.0057 – – – – – –
Cytoplasmic Foci 0.0391 0.0429 0.0418 0.0448 0.0364 0.0385 0.0651 0.0451 0.0703 0.0473 0.0458 0.0245 – – – – – –
Eisosomes 0.0005 0 0.0002 0 0.0003 0 0 0 0.0001 0 0.0002 0 – – – – – –
Endoplasmic Reticulum 0.0109 0.0058 0.0026 0.002 0.0031 0.0051 0.0032 0.006 0.0043 0.001 0.0009 0.0038 – – – – – –
Endosome 0.543 0.5525 0.5824 0.6515 0.5529 0.5538 0.5466 0.5826 0.5462 0.6217 0.5511 0.5477 – – – – – –
Golgi 0.0833 0.0693 0.0732 0.0479 0.0856 0.0769 0.1192 0.0929 0.1131 0.0839 0.1788 0.1488 – – – – – –
Lipid Particles 0.0352 0.0114 0.0151 0.0044 0.0239 0.0076 0.023 0.0094 0.0161 0.0247 0.0113 0.0097 – – – – – –
Mitochondria 0.024 0.0167 0.0172 0.0399 0.0992 0.0182 0.0435 0.0336 0.0596 0.0442 0.0669 0.0283 – – – – – –
None 0.0319 0.0045 0.0116 0.001 0.0027 0.0038 0.0381 0.007 0.0288 0.0002 0.0002 0.0179 – – – – – –
Nuclear Periphery 0.0185 0.0047 0.0023 0.0026 0.0047 0.0041 0.0039 0.0034 0.003 0.0012 0.0006 0.0051 – – – – – –
Nucleolus 0.0022 0.0003 0.0004 0.0002 0.0008 0.0004 0.0014 0.0006 0.0009 0.0001 0.0001 0.0002 – – – – – –
Nucleus 0.0117 0.0012 0.0007 0.0009 0.0027 0.001 0.0013 0.001 0.0016 0.0003 0.0002 0.0006 – – – – – –
Peroxisomes 0.0087 0.0013 0.0113 0.005 0.0082 0.0027 0.003 0.003 0.0054 0.0056 0.0055 0.0051 – – – – – –
Punctate Nuclear 0.0108 0.0005 0.0031 0.0003 0.0013 0.0022 0.0525 0.0002 0.0013 0.0001 0.0001 0.0003 – – – – – –
Vacuole 0.0753 0.1605 0.1357 0.1147 0.1227 0.1777 0.0384 0.1169 0.0667 0.1029 0.0746 0.1137 – – – – – –
Vacuole Periphery 0.0304 0.067 0.0735 0.0647 0.0283 0.089 0.0461 0.0806 0.0383 0.0571 0.0278 0.0852 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0771 16.0307 18.4501 19.6454 8.7782 24.5065 39.3637 40.2983 41.8377 40.6843
Translational Efficiency 0.9629 0.9036 0.6492 0.6464 0.6123 0.6583 0.7188 0.5978 0.5464 0.5741

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant
Localization
Cell Percentages vacuole (23%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ccc2

Ccc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available