Standard name
Human Ortholog
Description Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.06 0 0.2 0.23 0.29 0.24 0.23 0.27
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.13 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.22 0.25 0.29 0.23 0.25 0.17 0.18 0.19 0.2
Mitochondria 0.97 0.93 0.92 0.98 0.92 0.94 0.94 0.87 – 0.95 0.93 0.93 0.9 0.85 0.82 0.13 0.12 0.08 0.32 0.31 0.24 0.23 0.27 0.23
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.34 0.21 0.24 0.2 0.31 0.36 0.42 0.33 – 0.36 0.33 0.39 0.17 0.22 0.23 0.38 0.39 0.29 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.12 0 0 0.06 0.06 0.16 0.11 0.26 0.28 0.24 0.25
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.09 0.07 0.08 0.14 – 0.11 0.11 0.1 0 0 0.08 0.13 0.07 0.28 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 1 1 1 0 0 0 – 0 0 0 2 1 0 6 8 3 63 101 88 27 40 51
Bud 0 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Cytoplasm 0 2 4 1 0 4 3 6 – 7 6 5 0 0 0 8 17 1 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 9 6 2 1 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 0 3 6 1 0 1 1
Golgi 1 2 4 0 4 0 1 5 – 0 0 1 3 3 7 24 32 25 72 111 52 20 32 38
Mitochondria 283 384 232 169 169 279 302 288 – 258 240 296 129 79 90 14 16 7 99 138 73 26 46 44
Nucleus 0 1 0 0 0 0 2 1 – 0 0 1 0 0 0 2 1 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 99 86 60 35 57 108 136 109 – 97 85 126 25 20 25 41 51 25 11 12 3 1 4 5
Peroxisomes 1 4 2 3 2 1 1 2 – 0 0 1 9 11 3 4 8 5 50 50 79 32 42 48
SpindlePole 0 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 1 1 3 1 0 2 0
Vac/Vac Membrane 17 12 5 3 17 21 26 45 – 29 28 32 4 1 9 14 9 24 1 2 0 1 0 0
Unique Cell Count 293 414 252 173 183 297 321 331 273 259 320 143 93 110 107 130 85 312 445 307 114 175 192
Labelled Cell Count 401 502 308 212 250 413 471 457 392 359 462 172 117 134 125 151 94 312 445 307 114 175 192


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.8 57.6 55.2 58.0 70.2 63.5 74.8 88.6 – 97.1 92.8 105.7 55.6 50.7 54.0 47.8 52.6 66.0 61.8 61.7 68.9
Std Deviation (1e-4) 21.2 23.0 20.0 21.3 24.5 23.9 26.7 31.8 – 38.9 43.4 42.5 21.6 20.0 17.0 24.8 24.4 29.4 38.0 23.0 29.0
Intensity Change (Log2) – – – 0.07 0.35 0.2 0.44 0.68 – 0.81 0.75 0.94 0.01 -0.12 -0.03 -0.21 -0.07 0.26 0.16 0.16 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0.8 0 0 0 0 0 0 4.7 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 6.7 7.3 7.9
Mitochondria 2.5 0.1 0.9 1.0 -1.9 – 1.1 0.3 0.2 -0.6 -2.0 -2.9 -14.7 -15.5 -14.7
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 1.7 3.2 4.6 2.4 – 2.9 2.3 3.9 -1.5 -0.4 -0.2 2.8 3.1 1.0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 3.4 2.8 3.2 5.0 – 4.0 4.1 3.9 0 0 0 4.3 0 7.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 105.8836 142.4486 125.1742 97.3806 124.8824 114.0722 110.2638 147.9075 133.0089 116.2112 146.9907 131.726 154.0305 173.8711 169.9175 146.1897 166.3747 163.6104
Actin 0.0303 0.0066 0.013 0.0001 0.0002 0.0016 0.01 0.0008 0.0051 0.0001 0.0055 0.001 0.0007 0.0014 0.0055 0 0.001 0.0007
Bud 0.0005 0.0006 0.0059 0.0001 0 0.0126 0.0001 0 0 0 0 0 0 0 0.0001 0 0 0
Bud Neck 0.0002 0.0007 0.0002 0.0003 0 0.0002 0.0002 0 0 0 0 0 0 0.0002 0.0001 0 0 0
Bud Periphery 0.0006 0.0003 0.0059 0.0001 0 0.0111 0.0001 0 0 0.0001 0 0 0 0 0.0001 0 0 0
Bud Site 0.0023 0.0051 0.0015 0.0006 0 0.0002 0.0003 0 0 0 0.0001 0 0 0.0003 0.0006 0 0 0
Cell Periphery 0.0007 0.0049 0.0018 0.005 0.0001 0.0007 0.0002 0.0003 0.0002 0.0015 0 0.0004 0.0002 0.0006 0.0002 0 0.0001 0
Cytoplasm 0.0054 0.0001 0.0008 0 0 0.0283 0.0002 0 0 0 0 0.0001 0 0 0.0004 0 0 0
Cytoplasmic Foci 0.0031 0.0006 0.0007 0.0006 0.0001 0.0021 0.0109 0.0001 0.0013 0 0.0002 0.0002 0.0017 0.0005 0.0003 0 0.0002 0
Eisosomes 0.0048 0.0056 0.0074 0.0004 0.0005 0.0007 0.0023 0.0009 0.0021 0.0004 0.0017 0.0009 0.0056 0.0025 0.0023 0.0008 0.0016 0.0008
Endoplasmic Reticulum 0.0023 0 0.0012 0 0 0.0001 0.0001 0 0 0 0 0 0 0 0.0004 0 0 0
Endosome 0.0042 0.0003 0.0032 0.0001 0.0001 0.0001 0.001 0 0 0.0001 0 0.0001 0 0.0001 0.0005 0 0 0
Golgi 0.0027 0.0009 0.0017 0.0001 0.0007 0.0002 0.0012 0.0003 0.0004 0.0001 0.0007 0.0002 0.0002 0.0007 0.0003 0 0.0006 0.0001
Lipid Particles 0.0162 0.0153 0.023 0.0037 0.0199 0.0399 0.0236 0.0166 0.0245 0.0073 0.0035 0.007 0.0088 0.0255 0.005 0.0001 0.0319 0.007
Mitochondria 0.8569 0.9007 0.8686 0.5705 0.9692 0.8703 0.9142 0.9645 0.9395 0.9436 0.9681 0.9692 0.9644 0.9253 0.9746 0.9976 0.9508 0.9814
None 0.0066 0.0004 0.0005 0.0001 0 0.0205 0.0024 0 0.0001 0 0 0.0001 0.0003 0 0.0004 0 0 0
Nuclear Periphery 0.0062 0.0001 0.0079 0 0 0.0001 0.0004 0 0 0.0001 0 0.0003 0 0 0.0002 0 0 0
Nucleolus 0.024 0.0223 0.0056 0.3692 0.0003 0.0008 0.0051 0.0016 0.0127 0.0012 0.0001 0.0047 0.0089 0.0109 0.0008 0 0.0026 0.0001
Nucleus 0.0042 0.0017 0.0015 0.0165 0 0.0003 0.0005 0.0001 0.0005 0.0002 0 0.0009 0.0005 0.0005 0.0002 0 0.0001 0
Peroxisomes 0.007 0.0088 0.0106 0.0031 0.0017 0.0069 0.021 0.0077 0.009 0.0008 0.0196 0.0064 0.0049 0.012 0.0053 0.0014 0.0071 0.0038
Punctate Nuclear 0.0009 0.0008 0.0001 0 0 0.0001 0.0014 0 0 0 0 0 0 0 0.0002 0 0 0
Vacuole 0.0021 0.0052 0.0093 0.0088 0.0002 0.0009 0.0006 0.0009 0.0003 0.0053 0 0.001 0.0003 0.0019 0.0003 0 0.0001 0.0001
Vacuole Periphery 0.0187 0.0188 0.0296 0.0207 0.0071 0.0023 0.0044 0.0059 0.0042 0.0392 0.0004 0.0071 0.0033 0.0175 0.0023 0 0.0037 0.0058

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 408.7481 310.1691 342.6174 387.7151 367.4326 309.4427 384.8722 380.6057 386.1372 408.8042
Translational Efficiency 0.9079 1.0736 0.9534 0.9901 0.9544 1.3284 1.0296 0.9895 1.1708 0.9968

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
124 326 671 899 1564 541 1741 91 1688 867 2412 990

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 7943.97 5226.22 6812.08 5816.06 3116.80 4294.11 4743.04 6973.57 3471.40 4644.59 5318.63 5922.46
Standard Deviation 3932.84 1776.07 1870.15 2160.81 1157.47 1693.63 1491.08 2004.52 1990.86 1783.20 1854.02 2172.81
Intensity Change Log 2 — -0.604092 -0.221765 -0.449818 — 0.462294 0.605746 1.161832 — -0.216368 0.063080 0.209523

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.033752 0.026185 0.015853 0.023831 0.025852 0.037440 0.012725 0.032444 0.026432 0.033208 0.013596 0.024623
Bud Neck 0.010986 0.005263 0.004158 0.002646 0.002064 0.004346 0.004365 0.002460 0.002719 0.004691 0.004307 0.002629
Bud Site 0.008542 0.003510 0.001730 0.001797 0.001844 0.002737 0.005852 0.004252 0.002336 0.003028 0.004706 0.002022
Cell Periphery 0.000704 0.000089 0.000058 0.000529 0.000088 0.000090 0.000221 0.000338 0.000133 0.000089 0.000176 0.000511
Cytoplasm 0.000737 0.000577 0.000317 0.000583 0.000978 0.000828 0.000964 0.000377 0.000960 0.000733 0.000784 0.000564
Cytoplasmic Foci 0.046584 0.066103 0.067683 0.016067 0.060588 0.083162 0.044741 0.014134 0.059560 0.076748 0.051123 0.015889
Eisosomes 0.009922 0.018173 0.018120 0.015750 0.066134 0.032949 0.006420 0.009294 0.062004 0.027393 0.009675 0.015156
Endoplasmic Reticulum 0.000735 0.000151 0.000093 0.000148 0.000108 0.000186 0.000093 0.000443 0.000154 0.000173 0.000093 0.000175
Endosome 0.001984 0.000546 0.000310 0.000561 0.000227 0.000731 0.000939 0.000696 0.000356 0.000662 0.000764 0.000573
Golgi 0.032694 0.033054 0.020708 0.010815 0.016738 0.037313 0.013264 0.017017 0.017911 0.035712 0.015335 0.011385
Lipid Particles 0.013725 0.035115 0.021078 0.003559 0.018345 0.024924 0.015274 0.002837 0.018006 0.028756 0.016889 0.003492
Mitochondria 0.684809* 0.738027* 0.789513* 0.888505* 0.578326* 0.646307* 0.829707* 0.881872* 0.586148* 0.680794* 0.818525* 0.887895*
Mitotic Spindle 0.003117 0.001899 0.005148 0.000875 0.000727 0.002167 0.002547 0.001016 0.000903 0.002067 0.003271 0.000888
None 0.006204 0.005689 0.008649 0.004052 0.003800 0.003966 0.003323 0.006518 0.003976 0.004614 0.004804 0.004279
Nuclear Periphery 0.001629 0.000801 0.001937 0.000267 0.000162 0.000441 0.000732 0.000593 0.000270 0.000576 0.001068 0.000297
Nuclear Periphery Foci 0.000630 0.000218 0.000381 0.000080 0.000095 0.000224 0.000304 0.000147 0.000134 0.000222 0.000325 0.000087
Nucleolus 0.004001 0.004368 0.002804 0.001118 0.002158 0.003595 0.002344 0.002672 0.002293 0.003885 0.002472 0.001261
Nucleus 0.000391 0.000265 0.000140 0.000597 0.000200 0.000295 0.000497 0.000101 0.000214 0.000283 0.000398 0.000551
Peroxisomes 0.023207 0.047664 0.030996 0.016634 0.219738* 0.108224* 0.046227 0.005361 0.205301* 0.085453 0.041990 0.015598
Vacuole 0.048061 0.004066 0.004415 0.004606 0.001100 0.005971 0.004768 0.007945 0.004550 0.005255 0.004670 0.004913
Vacuole Periphery 0.067586 0.008238 0.005907 0.006982 0.000728 0.004105 0.004692 0.009480 0.005640 0.005659 0.005030 0.007212

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.68 1.67 0.91 0.51 -2.25 -2.36 5.32 -0.58 0.41 -1.76 -1.80 5.37 0.52 2.07 -3.69
Bud Neck 2.17 2.83 3.36 1.88 1.84 -3.16 -4.11 -0.54 2.21 2.66 -2.74 -3.07 0.03 2.43 2.49
Bud Site 1.44 2.03 1.97 1.24 -0.40 -0.97 -3.63 -0.69 -0.42 0.48 -0.80 -2.68 0.31 1.09 3.00
Cell Periphery 4.11 4.32 0.64 -2.09 -2.24 -0.14 -2.53 -1.92 -1.91 -0.76 2.36 -1.16 -1.98 -2.21 -1.69
Cytoplasm 0.76 2.01 -0.25 -0.65 -1.25 2.03 -0.14 2.39 1.78 2.31 3.50 1.76 0.34 -0.22 -0.05
Cytoplasmic Foci -1.57 -1.88 3.19 7.06 10.34 -3.68 4.28 8.14 9.12 5.44 -3.46 2.46 13.97 12.83 12.02
Eisosomes -2.23 -2.43 -1.71 0.91 1.08 8.08 19.62 16.48 7.29 -1.68 10.10 18.19 14.73 5.06 -3.52
Endoplasmic Reticulum 1.90 2.09 1.89 0.03 -1.47 -3.16 0.93 -1.49 -1.13 -1.55 -0.64 2.32 -0.48 -0.08 -2.02
Endosome 2.88 3.38 2.70 -0.09 -1.33 -5.31 -4.37 -1.22 0.09 0.60 -3.86 -3.25 -1.23 0.47 0.92
Golgi -0.05 1.63 3.03 4.83 4.30 -5.28 2.08 -0.05 2.93 -0.63 -5.74 1.63 3.61 7.78 2.42
Lipid Particles -3.03 -1.69 2.74 5.21 7.15 -1.93 1.48 7.03 6.42 5.91 -3.26 0.59 9.21 8.35 9.91
Mitochondria -1.60 -3.33 -6.56 -8.63 -8.15 -4.07 -23.78 -13.85 -9.54 -2.52 -6.93 -23.68 -28.72 -16.38 -8.34
Mitotic Spindle 0.99 -1.33 1.94 1.64 3.95 -2.11 -4.54 -0.62 1.55 3.05 -2.29 -5.37 -0.01 2.26 5.26
None 0.57 -2.61 2.61 3.25 7.51 -0.71 2.36 -2.00 -1.87 -2.34 -2.62 -3.51 -1.00 0.95 1.53
Nuclear Periphery 2.66 -0.62 4.53 5.28 4.34 -5.93 -4.89 -2.93 -0.99 0.75 -5.83 -5.77 -0.68 4.79 5.47
Nuclear Periphery Foci 2.12 1.23 2.83 4.24 4.24 -3.83 -3.11 -1.22 1.49 2.02 -2.99 -3.48 2.15 5.27 4.49
Nucleolus -0.18 1.20 2.98 2.09 3.55 -1.98 -0.58 -0.25 0.44 -0.15 -2.11 -0.57 2.65 3.43 3.78
Nucleus 1.03 2.44 -0.82 -1.15 -1.48 -0.99 -1.88 1.67 2.06 2.40 -0.96 -1.63 -1.26 -1.06 -0.58
Peroxisomes -2.46 -0.92 0.81 4.70 3.47 10.80 24.39 31.00* 12.34 11.12 14.35 24.75 29.00 11.68 8.51
Vacuole 4.35 4.31 4.22 -0.61 -0.37 -4.13 -6.56 -1.61 -0.45 -0.73 -0.63 -0.17 -0.45 0.01 -0.39
Vacuole Periphery 4.87 5.12 5.01 0.53 -0.78 -4.40 -9.22 -3.49 -2.05 -1.88 -0.02 0.56 -1.10 -1.13 -1.90
Standard name
Human Ortholog
Description Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
Localization
Cell Percentages mitochondrion (75%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Atp2

Atp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available