Standard name
Description Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0.2 0.23 0.29 0.24 0.23 0.27
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.22 0.25 0.29 0.23 0.25 0.17 0.18 0.19 0.2
Mitochondria 0.97 0.93 0.92 0.98 0.92 0.94 0.94 0.87 0.95 0.93 0.93 0.9 0.85 0.82 0.13 0.12 0.08 0.32 0.31 0.24 0.23 0.27 0.23
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.34 0.21 0.24 0.2 0.31 0.36 0.42 0.33 0.36 0.33 0.39 0.17 0.22 0.23 0.38 0.39 0.29 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0 0 0.06 0.06 0.16 0.11 0.26 0.28 0.24 0.25
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0.09 0.07 0.08 0.14 0.11 0.11 0.1 0 0 0.08 0.13 0.07 0.28 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 1 1 1 0 0 0 0 0 0 2 1 0 6 8 3 63 101 88 27 40 51
Bud 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Cytoplasm 0 2 4 1 0 4 3 6 7 6 5 0 0 0 8 17 1 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 9 6 2 1 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 3 6 1 0 1 1
Golgi 1 2 4 0 4 0 1 5 0 0 1 3 3 7 24 32 25 72 111 52 20 32 38
Mitochondria 283 384 232 169 169 279 302 288 258 240 296 129 79 90 14 16 7 99 138 73 26 46 44
Nucleus 0 1 0 0 0 0 2 1 0 0 1 0 0 0 2 1 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 99 86 60 35 57 108 136 109 97 85 126 25 20 25 41 51 25 11 12 3 1 4 5
Peroxisomes 1 4 2 3 2 1 1 2 0 0 1 9 11 3 4 8 5 50 50 79 32 42 48
SpindlePole 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 3 1 0 2 0
Vac/Vac Membrane 17 12 5 3 17 21 26 45 29 28 32 4 1 9 14 9 24 1 2 0 1 0 0
Unique Cell Count 293 414 252 173 183 297 321 331 273 259 320 143 93 110 107 130 85 312 445 307 114 175 192
Labelled Cell Count 401 502 308 212 250 413 471 457 392 359 462 172 117 134 125 151 94 312 445 307 114 175 192


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.8 57.6 55.2 58.0 70.2 63.5 74.8 88.6 97.1 92.8 105.7 55.6 50.7 54.0 47.8 52.6 66.0 61.8 61.7 68.9
Std Deviation (1e-4) 21.2 23.0 20.0 21.3 24.5 23.9 26.7 31.8 38.9 43.4 42.5 21.6 20.0 17.0 24.8 24.4 29.4 38.0 23.0 29.0
Intensity Change (Log2) 0.07 0.35 0.2 0.44 0.68 0.81 0.75 0.94 0.01 -0.12 -0.03 -0.21 -0.07 0.26 0.16 0.16 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.8 0 0 0 0 0 0 4.7 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 6.7 7.3 7.9
Mitochondria 2.5 0.1 0.9 1.0 -1.9 1.1 0.3 0.2 -0.6 -2.0 -2.9 -14.7 -15.5 -14.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 1.7 3.2 4.6 2.4 2.9 2.3 3.9 -1.5 -0.4 -0.2 2.8 3.1 1.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.4 2.8 3.2 5.0 4.0 4.1 3.9 0 0 0 4.3 0 7.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available