Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.07 0 0.08 0.1 0.09 0.05 0 0.06 0.05 0.06 0.05 0.22 0.19 0.12 0.1 0.11 0 0 0 0.08 0.08 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.06 0.08 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.27 0 0.06 0.36 0.16 0.17 0.19 0.57 0.38 0.55 0.47 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.76 0.66 0.73 0.83 0.65 0.63 0.72 0.74 0.63 0.68 0.66 0.68 0.84 0.72 0.67 0.71 0.62 0.67 0.61 0.63 0.59 0.41 0.36 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.05 0 0.05 0.12 0.06 0 0.06 0.05 0.06 0.05 0.05 0 0 0.05 0 0.08 0.08 0.16 0.08 0.11 0.07 0.07 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0.11 0.09 0.12 0.07
Vac/Vac Membrane 0.14 0.1 0.07 0 0 0.08 0.07 0.1 0 0 0.05 0.05 0.11 0.08 0.14 0.12 0.18 0.13 0.07 0.11 0.08 0.17 0.17 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 4 1 2 2 2 4 7 7 0 0 0 1 0 0 3 4 1 0 3 0
Bud Neck 0 0 1 0 0 1 2 0 0 3 2 1 0 0 0 0 0 0 0 3 0 0 2 0
Bud Site 0 0 0 0 1 0 1 3 5 5 7 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 32 11 5 5 13 19 13 12 20 13 23 3 8 8 30 23 23 1 12 3 7 13 6
Endoplasmic Reticulum 0 3 0 0 0 1 0 0 0 0 0 0 0 0 0 13 8 6 0 0 0 2 0 0
Endosome 1 3 14 5 1 2 2 4 1 1 2 3 0 0 0 9 10 8 16 18 8 4 12 23
Golgi 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Mitochondria 3 121 0 7 24 21 37 45 172 123 143 190 2 0 1 11 13 2 0 12 0 0 4 6
Nucleus 180 298 119 102 43 83 157 176 190 223 170 277 47 26 29 180 140 142 176 332 105 35 57 40
Nuclear Periphery 0 2 0 2 0 0 0 4 2 5 0 1 0 0 0 11 8 1 4 4 2 0 2 2
Nucleolus 25 24 7 6 8 8 7 14 14 21 12 22 1 1 2 9 19 17 45 42 19 5 10 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0
SpindlePole 0 5 16 2 2 2 1 6 5 4 0 2 0 0 0 3 4 12 15 25 19 7 20 10
Vac/Vac Membrane 33 45 12 4 2 11 16 23 12 12 14 19 6 3 6 30 41 28 19 58 14 14 27 37
Unique Cell Count 237 451 164 123 66 131 217 239 302 328 259 407 56 36 43 254 225 211 289 528 179 85 161 146
Labelled Cell Count 250 534 180 134 90 143 245 290 415 421 370 562 59 38 46 297 267 240 289 528 179 85 161 146


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 4.5 6.4 6.0 4.3 5.2 5.2 5.5 4.2 4.5 4.2 4.3 6.0 6.2 5.8 6.7 7.9 8.2 6.6 7.5 7.3
Std Deviation (1e-4) 1.0 0.9 1.0 1.0 1.1 1.4 1.1 1.5 0.9 1.0 0.6 0.8 1.0 1.1 1.5 1.7 2.0 1.8 1.6 1.9 1.5
Intensity Change (Log2) – – – -0.09 -0.57 -0.3 -0.31 -0.23 -0.63 -0.51 -0.63 -0.57 -0.11 -0.06 -0.14 0.06 0.3 0.35 0.04 0.21 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.5 3.1 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1051 4.1597 4.3964 4.1107 3.7789 4.2914 5.9061 6.2198 5.887 5.908 5.9123 5.7204 2.5288 2.9348 2.9336 3.1077 3.4125 2.7522
Actin 0.0268 0.0002 0.0095 0.0124 0.0193 0.0014 0.0229 0.0001 0.0061 0.003 0.0193 0.0002 0.0352 0.0002 0.0014 0.0002 0.002 0.0002
Bud 0.0007 0.0002 0.0003 0.0007 0.0005 0.0003 0.0007 0.0001 0.0006 0.0002 0.0006 0.0001 0.0003 0.0002 0.0001 0.0002 0.0002 0.0002
Bud Neck 0.0046 0.0011 0.0015 0.0016 0.0054 0.0151 0.0069 0.0019 0.0031 0.0022 0.003 0.0034 0.0006 0.0012 0.0013 0.0025 0.0034 0.0113
Bud Periphery 0.0014 0.0003 0.0008 0.0009 0.0007 0.0006 0.0012 0.0001 0.0011 0.0002 0.0015 0.0002 0.0007 0.0003 0.0002 0.0003 0.0003 0.0003
Bud Site 0.0056 0.0015 0.0021 0.0061 0.0029 0.0017 0.0043 0.0015 0.0086 0.0011 0.002 0.0004 0.0009 0.0033 0.0012 0.0015 0.0007 0.0012
Cell Periphery 0.0007 0.0002 0.0004 0.0004 0.0002 0.0002 0.0007 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0127 0.0088 0.0126 0.0123 0.0037 0.0115 0.0325 0.0073 0.0082 0.0045 0.0058 0.0067 0.0444 0.042 0.0028 0.059 0.004 0.0081
Cytoplasmic Foci 0.0064 0.0005 0.0015 0.0152 0.0121 0.0047 0.0042 0.0003 0.0041 0.0161 0.0049 0.0005 0.0092 0.0015 0.0029 0.0003 0.0507 0.0005
Eisosomes 0.0002 0 0.0002 0.0002 0.0003 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0095 0.002 0.0092 0.0117 0.0015 0.0017 0.0111 0.0006 0.0025 0.0013 0.0017 0.0013 0.0041 0.002 0.0003 0.0032 0.0006 0.0005
Endosome 0.0159 0.0026 0.0081 0.0395 0.0456 0.0112 0.0227 0.001 0.0131 0.0139 0.0065 0.0024 0.0122 0.0034 0.0023 0.0007 0.0428 0.002
Golgi 0.008 0.0001 0.0036 0.0137 0.0204 0.0017 0.0045 0 0.0034 0.0136 0.0045 0.0001 0.0134 0.0001 0.0007 0 0.0432 0.0003
Lipid Particles 0.0089 0.0012 0.004 0.0248 0.0228 0.0092 0.0068 0.0001 0.0076 0.0096 0.0034 0.0006 0.0233 0.0004 0.0033 0.0001 0.0081 0.0006
Mitochondria 0.0057 0.0004 0.0068 0.009 0.0215 0.0055 0.0039 0.0003 0.0026 0.004 0.006 0.0003 0.0047 0.0005 0.0005 0.0005 0.0031 0.0004
None 0.0054 0.002 0.0213 0.0031 0.001 0.0029 0.0285 0.0017 0.0093 0.006 0.0222 0.0105 0.0045 0.0258 0.0211 0.1157 0.0004 0.0191
Nuclear Periphery 0.0253 0.0238 0.0347 0.0311 0.0236 0.0369 0.0521 0.0132 0.0362 0.04 0.014 0.0056 0.033 0.023 0.0095 0.0082 0.0125 0.0097
Nucleolus 0.0198 0.0196 0.0425 0.0817 0.0573 0.0892 0.0559 0.0357 0.1065 0.1564 0.0702 0.0819 0.0095 0.022 0.0373 0.1329 0.0483 0.0688
Nucleus 0.8002 0.9132 0.8102 0.7218 0.712 0.7379 0.7083 0.909 0.7185 0.6082 0.7255 0.855 0.7697 0.8552 0.8656 0.6614 0.7519 0.8394
Peroxisomes 0.0061 0.0001 0.0008 0.0038 0.0125 0.0015 0.002 0 0.0019 0.0023 0.0007 0.0001 0.0077 0.0001 0.0179 0 0.0161 0.0001
Punctate Nuclear 0.0286 0.0188 0.022 0.0051 0.0326 0.0619 0.0203 0.0262 0.0611 0.114 0.1047 0.0293 0.0236 0.0161 0.0304 0.0119 0.01 0.0346
Vacuole 0.006 0.0029 0.0058 0.0031 0.0026 0.0034 0.0067 0.0008 0.0031 0.0025 0.0021 0.0012 0.0012 0.0019 0.001 0.001 0.0011 0.0022
Vacuole Periphery 0.0014 0.0004 0.0021 0.0016 0.0015 0.0016 0.0036 0.0002 0.0021 0.0008 0.0011 0.0002 0.0015 0.0004 0.0002 0.0002 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.9382 5.4078 13.6435 14.4023 14.9022 3.6276 15.5832 13.2854 12.9066 18.718
Translational Efficiency 0.8573 1.5712 0.8404 0.6691 0.9084 3.5863 0.6123 0.8394 0.8764 0.5297

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1700 610 714 1509 1825 2025 1684 1701 3525 2635 2398 3210

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 782.55 840.19 947.97 910.10 743.53 909.62 932.77 932.43 762.35 893.55 937.30 921.93
Standard Deviation 108.09 117.38 120.89 127.54 93.76 112.03 116.76 123.04 102.79 117.02 118.21 125.67
Intensity Change Log 2 — 0.102533 0.276658 0.217842 — 0.290873 0.327130 0.326604 — 0.197368 0.301470 0.271858

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000298 0.001309 0.001197 0.001396 0.000218 0.000281 0.000644 0.001084 0.000257 0.000519 0.000808 0.001231
Bud Neck 0.003028 0.012651 0.010700 0.031975 0.009225 0.010215 0.013911 0.029788 0.006236 0.010778 0.012955 0.030816
Bud Site 0.001308 0.003575 0.002364 0.013794 0.001975 0.002824 0.002119 0.011343 0.001653 0.002998 0.002192 0.012495
Cell Periphery 0.000105 0.000159 0.000098 0.000185 0.000122 0.000084 0.000087 0.000138 0.000114 0.000102 0.000090 0.000160
Cytoplasm 0.047014 0.057712 0.012499 0.035222 0.056564 0.040107 0.009541 0.029269 0.051958 0.044183 0.010422 0.032068
Cytoplasmic Foci 0.007279 0.009477 0.000601 0.003204 0.008685 0.008016 0.000169 0.001316 0.008007 0.008355 0.000298 0.002204
Eisosomes 0.000041 0.000063 0.000045 0.000035 0.000037 0.000031 0.000025 0.000032 0.000039 0.000038 0.000031 0.000033
Endoplasmic Reticulum 0.003363 0.010267 0.007050 0.012058 0.002378 0.003509 0.006781 0.010415 0.002853 0.005073 0.006861 0.011188
Endosome 0.001564 0.003356 0.000737 0.003971 0.002286 0.003472 0.001135 0.002612 0.001938 0.003445 0.001016 0.003251
Golgi 0.000750 0.002328 0.000165 0.001836 0.001096 0.001200 0.000391 0.000527 0.000929 0.001461 0.000324 0.001142
Lipid Particles 0.002999 0.003867 0.000607 0.000765 0.002885 0.002755 0.000249 0.000584 0.002940 0.003012 0.000356 0.000669
Mitochondria 0.004736 0.005178 0.001485 0.006271 0.003736 0.008299 0.001606 0.005682 0.004218 0.007576 0.001570 0.005959
Mitotic Spindle 0.004497 0.015472 0.002251 0.038441 0.006566 0.015116 0.003072 0.039118 0.005568 0.015198 0.002828 0.038800
None 0.015328 0.010662 0.005353 0.006565 0.017194 0.006644 0.005045 0.005303 0.016294 0.007574 0.005137 0.005896
Nuclear Periphery 0.003114 0.004187 0.001355 0.007663 0.002607 0.004064 0.001675 0.005485 0.002852 0.004092 0.001580 0.006509
Nuclear Periphery Foci 0.011682 0.019089 0.002421 0.003525 0.008337 0.006884 0.001323 0.003355 0.009950 0.009710 0.001650 0.003435
Nucleolus 0.107179 0.080300 0.052691 0.018078 0.148581 0.070133 0.034509 0.023367 0.128614 0.072487 0.039923 0.020880
Nucleus 0.781669* 0.747947* 0.893288* 0.795093* 0.723019* 0.808294* 0.912440* 0.816316* 0.751304* 0.794324* 0.906738* 0.806339*
Peroxisomes 0.001575 0.002417 0.000636 0.001552 0.001636 0.002998 0.000393 0.000634 0.001607 0.002864 0.000465 0.001065
Vacuole 0.002087 0.009271 0.004221 0.016123 0.002347 0.004295 0.004574 0.012160 0.002222 0.005447 0.004469 0.014023
Vacuole Periphery 0.000385 0.000714 0.000237 0.002248 0.000504 0.000778 0.000310 0.001473 0.000447 0.000763 0.000288 0.001837

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.07 -7.51 -7.69 0.47 0.68 -0.68 -10.12 -10.84 -10.99 -5.04 -4.87 -12.98 -13.09 -8.87 -3.34
Bud Neck -7.79 -9.77 -18.74 -10.62 -8.31 -1.41 -9.35 -16.63 -15.39 -8.50 -6.80 -14.25 -25.03 -19.44 -11.67
Bud Site -3.16 -2.74 -6.62 -3.31 -4.20 -1.15 -1.22 -7.14 -6.43 -7.05 -2.75 -2.94 -9.75 -7.45 -8.38
Cell Periphery -3.45 0.76 -2.14 1.07 -2.51 2.79 2.73 -0.96 -5.01 -4.93 1.17 2.91 -2.26 -3.32 -4.98
Cytoplasm -2.01 11.18 4.66 5.03 -8.12 3.70 14.05 8.25 4.95 -9.38 1.96 18.10 9.25 6.58 -12.54
Cytoplasmic Foci -0.52 8.93 7.56 5.38 -2.33 0.57 9.84 8.98 9.51 -4.05 -0.05 13.39 11.76 10.70 -4.44
Eisosomes -4.45 -1.48 1.72 5.45 2.94 2.40 2.49 -2.51 -4.78 -4.93 0.21 1.84 -0.57 -0.70 -2.41
Endoplasmic Reticulum -9.06 -10.71 -16.27 -1.86 -6.07 -3.89 -17.42 -21.33 -17.35 -8.89 -7.68 -19.54 -26.53 -16.84 -10.84
Endosome -2.93 3.73 -1.30 1.73 -3.61 -1.77 3.46 -0.15 1.65 -3.46 -2.82 4.16 -1.02 2.13 -4.51
Golgi -1.34 3.83 0.17 1.34 -1.58 -0.23 2.39 2.31 2.88 -0.52 -1.26 3.38 1.75 2.49 -1.46
Lipid Particles -1.16 8.92 8.63 6.28 -0.29 -0.08 6.63 5.51 6.52 -6.26 -0.35 10.71 9.42 8.61 -5.01
Mitochondria -0.39 3.06 -0.17 0.32 -6.47 -3.46 3.40 -1.90 2.46 -6.05 -3.06 4.17 -1.22 2.55 -8.51
Mitotic Spindle -3.76 -1.57 -8.96 -3.53 -7.11 -3.29 -0.43 -9.54 -6.86 -9.17 -5.27 -1.61 -13.09 -7.81 -11.57
None 2.65 7.85 6.77 3.20 -2.82 8.93 10.98 10.80 1.57 -1.90 9.11 13.16 12.33 2.59 -3.28
Nuclear Periphery -2.35 3.74 -9.29 -6.45 -12.64 -3.59 0.40 -10.47 -6.87 -11.18 -3.82 2.52 -13.92 -9.46 -16.42
Nuclear Periphery Foci -2.07 5.02 5.72 5.22 0.23 1.36 6.11 3.74 2.21 -4.18 0.61 8.40 6.75 5.19 -3.03
Nucleolus 4.31 7.01 15.93 8.22 6.49 11.93 17.36 19.71 8.78 2.28 11.86 18.32 25.20 12.41 5.95
Nucleus 2.56 -9.10 -2.15 -4.15 7.28 -9.22 -20.35 -6.63 2.67 13.82 -5.90 -21.61 -6.27 0.20 15.74
Peroxisomes -1.68 2.81 2.28 4.03 0.09 -2.26 4.40 4.43 5.16 -0.73 -2.76 5.18 4.55 5.71 -0.61
Vacuole -4.99 -4.42 -11.16 -5.20 -8.12 -3.53 -4.87 -11.91 -10.67 -9.44 -6.05 -6.43 -16.31 -12.94 -12.47
Vacuole Periphery -3.29 1.41 -2.13 -0.98 -2.48 -2.09 2.53 -0.95 -0.09 -1.61 -3.18 2.61 -2.13 -0.65 -2.82
Standard name
Human Ortholog
Description Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Nse3

Nse3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nse3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available