Standard name | |
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Description | Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 1 | 6 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 5 | 7 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 10 | 2 | 4 | 3 | 14 | 18 | 17 | 14 | 10 | 32 | 23 | 15 | 38 | 48 | 33 | 23 | 24 | 0 | 0 | 0 | 13 | 23 | 11 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 13 | 12 | 15 | 0 | 1 | 0 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 3 |
Golgi | 0 | 1 | 9 | 0 | 4 | 19 | 6 | 15 | 2 | 24 | 18 | 15 | 14 | 20 | 20 | 9 | 18 | 21 | 0 | 0 | 0 | 2 | 2 | 4 |
Mitochondria | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 16 | 4 | 1 | 5 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 3 |
Nucleus | 238 | 221 | 157 | 77 | 62 | 73 | 76 | 86 | 76 | 80 | 128 | 133 | 120 | 86 | 88 | 103 | 145 | 101 | 296 | 275 | 192 | 279 | 186 | 185 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 3 |
Nucleolus | 19 | 13 | 12 | 4 | 7 | 2 | 15 | 6 | 25 | 10 | 19 | 29 | 12 | 8 | 9 | 13 | 23 | 14 | 27 | 23 | 40 | 23 | 24 | 34 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 39 | 46 | 21 | 20 | 64 | 72 | 59 | 79 | 46 | 68 | 87 | 117 | 60 | 78 | 96 | 109 | 100 | 109 | 22 | 27 | 19 | 48 | 47 | 81 |
Unique Cell Count | 293 | 278 | 195 | 104 | 130 | 159 | 163 | 188 | 170 | 182 | 274 | 308 | 213 | 221 | 249 | 258 | 302 | 260 | 357 | 340 | 261 | 383 | 305 | 348 |
Labelled Cell Count | 303 | 291 | 201 | 106 | 141 | 184 | 175 | 207 | 186 | 198 | 296 | 328 | 221 | 230 | 265 | 287 | 322 | 285 | 357 | 340 | 261 | 383 | 305 | 348 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 49.8 | 36.0 | 37.0 | 32.8 | 27.1 | 30.9 | 29.1 | 27.9 | 24.5 | 27.0 | 26.5 | 25.0 | 39.9 | 41.6 | 39.1 | 33.1 | 41.1 | 38.6 | 37.9 | 40.4 | 41.4 |
Std Deviation (1e-4) | 10.8 | 9.3 | 8.2 | 7.6 | 8.5 | 8.7 | 7.4 | 6.8 | 7.8 | 7.0 | 7.8 | 6.9 | 10.4 | 11.6 | 11.2 | 13.0 | 14.3 | 16.4 | 11.1 | 13.0 | 13.7 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.45 | -0.26 | -0.35 | -0.41 | -0.59 | -0.45 | -0.48 | -0.56 | 0.11 | 0.17 | 0.08 | -0.16 | 0.15 | 0.06 | 0.03 | 0.13 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 3.5 | 4.1 | 3.6 | 3.4 | 2.5 | 4.4 | 3.2 | 3.0 | 5.6 | 6.0 | 4.6 | 3.3 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | -0.7 | 2.5 | -0.4 | 1.4 | -1.9 | 2.9 | 0.9 | 0.1 | 0.9 | 1.8 | 1.4 | -0.6 | 0.6 | 1.5 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.3 | -6.2 | -6.8 | -6.7 | -7.1 | -7.1 | -7.3 | -7.4 | -8.3 | -5.2 | -8.6 | -9.5 | -8.7 | -7.2 | -8.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.8 | -0.3 | -2.4 | 1.1 | -1.4 | 2.7 | -0.3 | 0.3 | 1.3 | -0.2 | -1.2 | -1.3 | -0.5 | 0.6 | -0.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.0 | 7.7 | 7.3 | 5.8 | 7.0 | 4.0 | 6.1 | 5.3 | 6.7 | 4.4 | 5.9 | 6.6 | 7.3 | 5.7 | 7.3 |