Standard name
Description Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.09 0.11 0.09 0.08 0.05 0.12 0.07 0.07 0.17 0.19 0.13 0.08 0.09 0 0 0 0 0.08 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0 0 0.12 0 0.08 0 0.13 0.07 0.05 0.07 0.09 0.08 0 0.06 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.81 0.79 0.81 0.74 0.48 0.46 0.47 0.46 0.45 0.44 0.47 0.43 0.56 0.39 0.35 0.4 0.48 0.39 0.83 0.81 0.74 0.73 0.61 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0.06 0 0.05 0 0.09 0 0.15 0.05 0.07 0.09 0.06 0 0 0.05 0.08 0.05 0.08 0.07 0.16 0.06 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.17 0.11 0.19 0.49 0.45 0.36 0.42 0.27 0.37 0.32 0.38 0.28 0.35 0.39 0.42 0.33 0.42 0.06 0.08 0.08 0.13 0.15 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 1 2 1 1 6 0 0 0 3 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 3 3 3 5 7 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 3 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 10 2 4 3 14 18 17 14 10 32 23 15 38 48 33 23 24 0 0 0 13 23 11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1 2 1 0 0 0 13 12 15 0 1 0 0 2 1
Endosome 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 0 0 1 1 2 1 3
Golgi 0 1 9 0 4 19 6 15 2 24 18 15 14 20 20 9 18 21 0 0 0 2 2 4
Mitochondria 2 0 0 1 1 0 0 1 16 4 1 5 0 0 0 5 0 1 0 1 0 1 2 3
Nucleus 238 221 157 77 62 73 76 86 76 80 128 133 120 86 88 103 145 101 296 275 192 279 186 185
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 1 1 1 1 1 3
Nucleolus 19 13 12 4 7 2 15 6 25 10 19 29 12 8 9 13 23 14 27 23 40 23 24 34
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 1
Vac/Vac Membrane 39 46 21 20 64 72 59 79 46 68 87 117 60 78 96 109 100 109 22 27 19 48 47 81
Unique Cell Count 293 278 195 104 130 159 163 188 170 182 274 308 213 221 249 258 302 260 357 340 261 383 305 348
Labelled Cell Count 303 291 201 106 141 184 175 207 186 198 296 328 221 230 265 287 322 285 357 340 261 383 305 348


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 49.8 36.0 37.0 32.8 27.1 30.9 29.1 27.9 24.5 27.0 26.5 25.0 39.9 41.6 39.1 33.1 41.1 38.6 37.9 40.4 41.4
Std Deviation (1e-4) 10.8 9.3 8.2 7.6 8.5 8.7 7.4 6.8 7.8 7.0 7.8 6.9 10.4 11.6 11.2 13.0 14.3 16.4 11.1 13.0 13.7
Intensity Change (Log2) – – – -0.17 -0.45 -0.26 -0.35 -0.41 -0.59 -0.45 -0.48 -0.56 0.11 0.17 0.08 -0.16 0.15 0.06 0.03 0.13 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.5 4.1 3.6 3.4 2.5 4.4 3.2 3.0 5.6 6.0 4.6 3.3 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 0 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 -0.7 2.5 -0.4 1.4 -1.9 2.9 0.9 0.1 0.9 1.8 1.4 -0.6 0.6 1.5
Mitochondria 0 0 0 0 0 4.4 0 0 0 0 0 0 0 0 0
Nucleus -1.3 -6.2 -6.8 -6.7 -7.1 -7.1 -7.3 -7.4 -8.3 -5.2 -8.6 -9.5 -8.7 -7.2 -8.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.8 -0.3 -2.4 1.1 -1.4 2.7 -0.3 0.3 1.3 -0.2 -1.2 -1.3 -0.5 0.6 -0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 7.7 7.3 5.8 7.0 4.0 6.1 5.3 6.7 4.4 5.9 6.6 7.3 5.7 7.3