Standard name
Human Ortholog
Description Transmembrane histidine phosphotransfer kinase and osmosensor; regulates MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.29 0.16 0.19 0 0 0 0 0 0 0.06 0 0.12 0.07 0.09 0.1 0.06 0.08 0.2 0.19 0.25 0.25 0.22 0.2
Bud 0 0.11 0 0.07 0 0.07 0.06 0.06 0.09 0.06 0.08 0.06 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0.09 0 0.08 0.09 0.12
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.32 0.33 0.42 0.35 0.21 0.22 0.16 0.11 0.17 0.12 0.13 0.15 0.28 0.3 0.33 0.29 0.29 0.25 0.53 0.54 0.61 0.41 0.5 0.49
Cytoplasm 0.61 0.52 0.45 0.43 0.65 0.53 0.52 0.47 0.45 0.43 0.34 0.45 0.86 0.84 0.84 0.65 0.67 0.6 0 0 0 0.07 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0.09 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.09 0.06 0.17 0.14 0.36 0.44 0.47 0.55 0.57 0.62 0.58 0 0 0 0.13 0.11 0.09 0 0 0 0.1 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.19 0.14 0.15 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 73 64 59 50 7 17 8 15 12 13 24 2 10 13 15 10 4 6 122 53 107 47 73 63
Bud 17 24 13 19 12 34 37 44 45 35 33 28 1 1 1 3 3 4 4 2 3 1 2 3
Bud Neck 8 0 4 1 0 0 0 0 0 0 0 0 2 2 1 2 4 0 47 24 17 15 29 37
Bud Site 2 2 0 7 1 1 1 2 3 5 2 2 0 0 0 0 0 0
Cell Periphery 153 72 153 92 66 108 98 80 85 74 56 69 24 55 52 30 19 19 320 153 266 78 167 153
Cytoplasm 286 114 163 114 204 265 308 327 227 257 147 212 73 157 134 68 44 45 27 12 3 12 11 7
Endoplasmic Reticulum 6 3 4 8 4 18 15 25 12 23 29 6 0 1 3 5 6 5 23 12 10 5 8 5
Endosome 2 0 0 0 10 0 3 7 0 2 0 4 0 1 0 1 0 1 5 3 1 2 2 4
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 2 3 0 2 0 0 1
Mitochondria 23 20 23 46 44 181 260 327 275 341 264 272 1 0 0 14 7 7 26 11 12 19 24 22
Nucleus 0 0 1 1 0 0 0 5 1 0 0 2 1 0 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 0 0 2 0 2 1 1 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 9 1 68 38 47 32 22 34 14 22 3 13 0 0 0 0 0 0 0 0 1 1 0 1
SpindlePole 22 3 18 12 8 6 9 14 8 6 3 7 0 2 3 6 0 4 0 1 0 0 0 0
Vac/Vac Membrane 2 0 0 0 3 0 1 3 0 1 0 2 0 0 0 0 0 2 12 5 0 2 5 5
Unique Cell Count 471 219 364 265 314 497 596 699 504 600 427 466 85 186 160 105 66 75 601 284 434 193 332 312
Labelled Cell Count 604 304 506 390 406 662 763 885 682 781 563 621 112 232 210 141 87 96 601 284 434 193 332 312


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.5 5.3 5.1 5.0 4.2 4.0 4.2 3.9 3.7 3.7 3.7 6.0 6.3 6.4 4.9 5.7 6.4 6.2 6.3 6.7
Std Deviation (1e-4) 1.0 0.8 1.5 1.7 1.7 1.1 1.0 0.9 0.8 0.8 0.6 0.6 1.0 0.9 0.7 1.5 1.7 1.6 1.4 1.5 1.9
Intensity Change (Log2) -0.07 -0.09 -0.33 -0.39 -0.33 -0.43 -0.53 -0.52 -0.53 0.17 0.24 0.27 -0.11 0.11 0.28 0.22 0.24 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 -6.1 0 0 0 0 0 0 0 -1.0 -3.0 -2.1 -1.7 -2.1 -1.8
Bud 2.0 0.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.9 -5.8 0 0 0 0 0 0 0 -2.3 -2.9 -2.1 -2.5 -2.0 -2.7
Cytoplasm -0.4 5.3 2.5 2.1 0.6 0.1 -0.6 -3.0 0.2 6.8 8.9 8.3 3.6 3.3 2.4
Endoplasmic Reticulum 1.7 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.4 3.3 0 0 0 0 0 0 0 0 -3.5 -3.3 2.3 0 0.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.4 -1.3 0 0 0 0 0 0 0 -4.3 -6.3 -5.9 -4.8 -3.8 -4.1
SpindlePole -0.2 -1.6 0 0 0 0 0 0 0 0 -2.3 -1.7 0.3 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3937 1.7873 1.7293 1.4321 1.6498 1.6246 2.9327 3.575 3.3297 2.9751 3.2082 3.4134 2.525 3.4311 3.1233 3.916 2.993 3.2186
Actin 0.084 0.0387 0.0476 0.0808 0.0344 0.0143 0.0907 0.0102 0.0399 0.0343 0.0429 0.0317 0.031 0.003 0.0201 0.0212 0.0038 0.035
Bud 0.0031 0.0014 0.0012 0.0013 0.0015 0.0016 0.0019 0.0005 0.0177 0.002 0.0008 0.0016 0.0003 0.0003 0.0008 0.0006 0.0012 0.0008
Bud Neck 0.0141 0.0097 0.0776 0.0576 0.0592 0.0353 0.0165 0.0088 0.053 0.0707 0.0971 0.0512 0.0053 0.0065 0.0571 0.0888 0.0427 0.0499
Bud Periphery 0.018 0.0031 0.0061 0.0077 0.0103 0.0159 0.0067 0.0022 0.0127 0.0158 0.0112 0.0113 0.0016 0.0016 0.0067 0.005 0.0071 0.0099
Bud Site 0.0467 0.1931 0.1112 0.0164 0.0092 0.0028 0.1023 0.2273 0.1173 0.0194 0.0058 0.0067 0.0273 0.1488 0.0789 0.0203 0.0105 0.0049
Cell Periphery 0.2019 0.2057 0.2407 0.2697 0.224 0.278 0.2338 0.1986 0.2231 0.3304 0.3138 0.255 0.2315 0.2571 0.2845 0.2716 0.3544 0.3162
Cytoplasm 0.3569 0.4448 0.3777 0.4994 0.3194 0.5326 0.346 0.4111 0.2872 0.4244 0.4111 0.4957 0.4957 0.4743 0.3481 0.4866 0.3586 0.4557
Cytoplasmic Foci 0.0337 0.0098 0.0149 0.0027 0.0193 0.0028 0.0302 0.0047 0.0201 0.0024 0.0283 0.0063 0.0448 0.0011 0.0098 0.0575 0.0126 0.0045
Eisosomes 0.0291 0.0076 0.0068 0.0036 0.0167 0.0036 0.0103 0.0034 0.017 0.0125 0.0055 0.0036 0.0063 0.0031 0.006 0.0017 0.0028 0.0037
Endoplasmic Reticulum 0.0076 0.0039 0.004 0.0038 0.004 0.0032 0.0034 0.0024 0.0039 0.0033 0.0025 0.0082 0.0014 0.0017 0.007 0.0025 0.0024 0.004
Endosome 0.0221 0.002 0.0045 0.0006 0.0277 0.0013 0.008 0.0016 0.0035 0.0003 0.0085 0.0028 0.0011 0.0001 0.0175 0.002 0.0003 0.0052
Golgi 0.015 0.0048 0.0063 0.0026 0.0264 0.0014 0.0106 0.0005 0.0067 0.0006 0.0029 0.0025 0.0033 0.0003 0.0181 0.0025 0.0004 0.0073
Lipid Particles 0.0178 0.0023 0.0035 0.0013 0.0027 0.0012 0.0106 0.005 0.0115 0.0006 0.0036 0.0033 0.0064 0.0003 0.0102 0.0059 0.0012 0.0104
Mitochondria 0.01 0.0008 0.0115 0.0003 0.0136 0.001 0.0046 0.0002 0.0055 0.0001 0.0002 0.0014 0.0009 0 0.0118 0.0004 0.0004 0.0026
None 0.0958 0.0693 0.0792 0.0494 0.2244 0.1024 0.1031 0.1133 0.143 0.0807 0.0406 0.1119 0.0032 0.1006 0.1152 0.0141 0.1946 0.0817
Nuclear Periphery 0.0025 0.0003 0.0007 0.0003 0.0004 0.0003 0.0011 0.0002 0.0005 0.0003 0.0006 0.0004 0.0001 0.0001 0.0007 0.0001 0.0002 0.0006
Nucleolus 0.0016 0.0003 0.0003 0.0001 0.0002 0.0002 0.0008 0.0056 0.001 0.0002 0.0004 0.0005 0 0.0001 0.0003 0.0002 0.0006 0.0001
Nucleus 0.0021 0.0004 0.0005 0.0003 0.0005 0.0003 0.0008 0.0008 0.0019 0.0004 0.0008 0.0008 0.0001 0.0002 0.0004 0.0003 0.0008 0.0003
Peroxisomes 0.026 0.0006 0.0034 0.0006 0.0028 0.0002 0.0079 0.0006 0.0306 0.0001 0.0029 0.0008 0.139 0 0.0038 0.0174 0.0013 0.0049
Punctate Nuclear 0.0054 0.0004 0.0006 0.0002 0.0005 0.0002 0.0065 0.0004 0.0024 0.0001 0.0194 0.0007 0.0003 0.0001 0.0004 0.0005 0.0021 0.0005
Vacuole 0.0055 0.0011 0.0015 0.0014 0.0018 0.0014 0.0037 0.0026 0.0016 0.0016 0.0009 0.0035 0.0003 0.0006 0.0021 0.0007 0.0019 0.0015
Vacuole Periphery 0.0011 0.0001 0.0002 0 0.0009 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0 0 0.0004 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.1056 29.3516 24.3778 25.4797 30.854 31.6116 29.269 29.4022 26.7146 41.9269
Translational Efficiency 0.5778 0.3562 0.3629 0.3411 0.3665 0.5757 0.4028 0.2937 0.3639 0.3142

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2233 804 259 1468 1538 1323 173 2055 3771 2127 432 3523

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 781.13 800.48 938.27 895.92 578.87 783.68 933.87 864.94 698.64 790.03 936.51 877.85
Standard Deviation 86.68 101.54 100.81 122.05 69.37 100.51 89.08 108.04 127.64 101.23 96.31 115.10
Intensity Change Log 2 0.035303 0.264440 0.197807 0.437025 0.689982 0.579361 0.220111 0.461082 0.372674

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.036920 0.052271 0.049655 0.111762* 0.024814 0.021061 0.039493 0.056066 0.031983 0.032858 0.045586 0.079274
Bud Neck 0.064940 0.085523 0.037406 0.057139 0.058926 0.088624 0.045245 0.064736 0.062487 0.087452 0.040545 0.061570
Bud Site 0.056068 0.068587 0.098999 0.137740* 0.035763 0.033599 0.113316* 0.100623* 0.047787 0.046824 0.104733* 0.116089*
Cell Periphery 0.053889 0.098812 0.076280 0.154728* 0.068433 0.079478 0.087208 0.087445 0.059821 0.086786 0.080656 0.115481*
Cytoplasm 0.415572* 0.422909* 0.225446* 0.199965* 0.390694* 0.454791* 0.236439* 0.372199* 0.405426* 0.442740* 0.229848* 0.300431*
Cytoplasmic Foci 0.025655 0.050570 0.126375 0.010074 0.064530 0.055436 0.059657 0.037765 0.041510 0.053596 0.099657 0.026226
Eisosomes 0.088693 0.111848* 0.031472 0.132708* 0.092709 0.122920* 0.042986 0.098011 0.090331 0.118735* 0.036083 0.112469*
Endoplasmic Reticulum 0.004803 0.004702 0.053368 0.013646 0.010412 0.003713 0.044179 0.004310 0.007090 0.004086 0.049689 0.008200
Endosome 0.001709 0.006638 0.007790 0.002100 0.007099 0.009259 0.003019 0.003086 0.003907 0.008268 0.005880 0.002675
Golgi 0.002878 0.022500 0.004559 0.001564 0.039455 0.023698 0.002430 0.003282 0.017796 0.023245 0.003707 0.002566
Lipid Particles 0.004514 0.009153 0.016972 0.003180 0.037217 0.018999 0.009601 0.008086 0.017852 0.015277 0.014020 0.006042
Mitochondria 0.002133 0.007813 0.000329 0.001195 0.058240 0.013278 0.000358 0.001203 0.025016 0.011212 0.000341 0.001200
Mitotic Spindle 0.001132 0.000097 0.000224 0.000425 0.000121 0.000359 0.000266 0.001391 0.000720 0.000260 0.000241 0.000989
None 0.000597 0.000618 0.000251 0.000452 0.004120 0.000582 0.000201 0.000725 0.002034 0.000596 0.000231 0.000611
Nuclear Periphery 0.000101 0.000062 0.000467 0.000097 0.000100 0.000121 0.000365 0.000132 0.000101 0.000099 0.000426 0.000118
Nuclear Periphery Foci 0.000410 0.000330 0.006132 0.000848 0.000361 0.000239 0.003420 0.000612 0.000390 0.000273 0.005046 0.000710
Nucleolus 0.000098 0.000086 0.000222 0.000055 0.000254 0.000309 0.000091 0.000149 0.000161 0.000225 0.000169 0.000110
Nucleus 0.174253* 0.017867 0.135756* 0.085020 0.013049 0.026479 0.192396* 0.086889 0.108506 0.023224 0.158438* 0.086110
Peroxisomes 0.004464 0.019240 0.004895 0.001721 0.086301 0.025664 0.002623 0.004123 0.037841 0.023236 0.003985 0.003122
Vacuole 0.061057 0.019787 0.123215 0.084733 0.006116 0.020231 0.116638 0.068716 0.038649 0.020063 0.120581 0.075390
Vacuole Periphery 0.000114 0.000588 0.000186 0.000848 0.001285 0.001160 0.000069 0.000451 0.000592 0.000944 0.000139 0.000617

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.85 -1.79 -15.57 -10.40 -7.99 1.71 -2.22 -10.36 -12.07 -2.55 -0.47 -2.72 -17.21 -15.91 -6.58
Bud Neck -3.92 5.60 2.57 5.53 -3.88 -6.22 2.16 -1.36 5.51 -3.00 -7.23 5.57 0.61 7.83 -5.27
Bud Site -2.64 -4.32 -13.80 -10.41 -3.76 0.72 -5.76 -13.71 -14.01 0.91 0.30 -7.11 -17.99 -17.67 -1.44
Cell Periphery -7.51 -2.44 -18.60 -6.97 -8.32 -1.96 -1.50 -3.62 -1.35 -0.26 -6.98 -2.81 -15.69 -6.07 -5.30
Cytoplasm -0.84 16.06 30.12 22.45 1.92 -7.36 11.03 2.38 9.78 -9.81 -5.96 19.43 19.00 21.59 -7.80
Cytoplasmic Foci -7.22 -10.93 10.50 12.20 12.66 2.53 0.78 8.17 5.07 3.57 -4.99 -9.33 8.28 11.19 11.69
Eisosomes -3.90 11.91 -7.80 -2.50 -17.13 -5.39 7.16 -0.91 4.65 -7.73 -6.98 13.49 -5.94 1.94 -17.46
Endoplasmic Reticulum 0.14 -9.02 -8.93 -8.47 7.19 6.56 -5.35 5.94 -1.44 6.38 5.47 -10.38 -2.05 -8.19 10.07
Endosome -6.06 -6.72 -0.80 5.09 5.86 -2.13 5.86 6.19 6.92 -0.25 -6.62 -3.22 3.39 8.59 5.22
Golgi -13.59 -2.37 3.27 14.44 4.26 8.36 24.25 23.89 16.64 -1.67 -4.79 17.74 20.92 22.05 2.26
Lipid Particles -5.78 -9.06 2.44 7.01 9.79 8.31 13.60 14.94 7.61 1.35 2.23 3.00 12.73 9.75 7.92
Mitochondria -6.59 4.38 1.74 7.84 -2.24 15.38 22.00 21.64 9.29 -3.27 9.45 20.76 19.81 11.48 -4.01
Mitotic Spindle 2.39 2.09 1.45 -2.17 -1.39 -1.18 -1.05 -3.45 -2.44 -2.90 1.57 1.82 -0.89 -2.85 -3.29
None -0.09 3.52 0.87 0.72 -1.13 4.69 5.21 4.40 -0.71 -2.82 4.48 5.75 4.27 -0.09 -2.86
Nuclear Periphery 4.82 -7.37 0.14 -4.03 7.35 -1.05 -6.59 -2.06 -0.61 5.64 0.18 -9.59 -2.08 -1.56 8.87
Nuclear Periphery Foci 1.62 -11.57 -6.16 -6.77 10.46 0.79 -8.61 -1.75 -6.33 8.47 1.72 -13.88 -4.58 -9.36 12.91
Nucleolus 0.72 -3.63 1.47 1.06 4.14 -0.31 2.20 1.26 0.98 -1.51 -0.61 -0.20 1.28 1.12 1.86
Nucleus 38.25* 3.68 16.79 -18.70 5.38 -7.95 -13.34 -27.65 -20.57 7.76 29.74 -6.33 6.43 -27.48 9.19
Peroxisomes -10.94 -0.52 6.67 13.38 4.65 17.39* 26.84* 26.40* 12.99 -2.75 8.02 22.60 23.86 17.59 1.68
Vacuole 14.98 -7.67 -6.82 -16.86 3.65 -9.47 -11.98 -26.48 -18.75 5.16 10.86 -13.39 -15.58 -24.93 6.79
Vacuole Periphery -6.23 -2.34 -2.04 -0.66 -1.82 0.49 14.47 4.45 2.43 -2.29 -2.26 11.24 -0.09 1.46 -2.66