Standard name
Description ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0.11 0.07 0.06 0 – 0.05 0.06 0.05 0.1 0 0 0 0.06 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0.07 0 0.05 – 0 0.06 0 0.08 0.09 0.14 0.14 0.25 – – 0 0 0 0 0 0
Cytoplasm 0 0.07 0.07 0.15 0.09 0.15 0.14 – 0.21 0.14 0.26 0.14 0 0.07 0.05 0.19 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.76 0.6 0.64 0.37 0.67 0.44 0.41 – 0.5 0.54 0.4 0.39 0.92 0.84 0.85 0.06 – – 0.84 0.8 0.87 0.9 0.83 0.86
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0.11 0 0.13 0.09 0.15 0 0.05 – 0 0 0 0 0.06 0 0 0.31 – – 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.13 0 0.28 0 – 0.19 0.16 0.19 0.27 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 – – 0 0 0 0 0 0
Nuclear Periphery 0.29 0.06 0.1 0 0 0 0 – 0 0 0 0.06 0.12 0.1 0.05 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.35 0.3 0.3 0.23 0.27 0.42 – 0.21 0.18 0.15 0.21 0 0 0.05 0.12 – – 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 7 13 13 10 3 – 8 11 10 27 5 7 5 1 – – 1 2 3 1 6 4
Bud 0 0 1 1 2 3 1 – 1 2 4 6 0 0 0 0 – – 0 2 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 1 1 5 4 12 6 10 – 5 12 9 21 16 28 30 4 – – 0 0 0 0 4 3
Cytoplasm 1 13 15 17 16 26 29 – 35 27 57 37 6 13 11 3 – – 0 6 1 1 1 5
Endoplasmic Reticulum 42 114 142 43 124 74 87 – 84 104 87 102 162 166 177 1 – – 81 188 226 114 165 186
Endosome 0 0 1 1 0 0 2 – 2 0 0 3 0 0 1 0 – – 0 0 2 0 1 0
Golgi 6 3 29 11 27 1 10 – 2 1 2 2 11 7 6 5 – – 3 3 4 0 3 4
Mitochondria 6 3 1 15 2 47 4 – 32 30 41 72 3 1 0 0 – – 2 4 5 3 6 1
Nucleus 0 0 0 1 1 4 4 – 1 4 5 5 0 0 0 1 – – 0 0 0 0 0 1
Nuclear Periphery 16 11 22 4 1 3 8 – 3 3 5 16 21 19 10 0 – – 3 8 7 1 1 0
Nucleolus 1 0 0 1 0 0 0 – 0 1 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 10 66 67 35 42 46 89 – 35 34 33 56 4 7 10 2 – – 0 13 5 0 3 5
Unique Cell Count 55 190 222 117 184 169 212 169 191 218 263 177 198 209 16 97 236 261 128 198 217
Labelled Cell Count 86 211 290 146 240 220 247 208 229 253 347 228 248 250 17 97 236 261 128 198 217


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 15.4 15.0 13.9 14.2 9.1 12.7 – 10.9 10.7 10.5 10.4 14.8 15.7 14.8 19.9 – – 13.7 15.3 15.9
Std Deviation (1e-4) 3.3 3.0 2.7 3.2 2.4 2.4 2.7 – 2.3 2.3 2.5 2.1 2.4 2.8 2.2 4.3 – – 2.4 2.9 2.9
Intensity Change (Log2) – – – -0.11 -0.08 -0.72 -0.24 – -0.46 -0.48 -0.51 -0.53 -0.01 0.06 -0.02 0.41 – – -0.13 0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.0 1.8 1.3 0 – 0.8 1.3 0.8 3.1 -0.2 0.2 -0.5 0 – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 2.1 0 1.4 – 0 2.1 1.1 2.8 3.0 4.5 4.6 0 – –
Cytoplasm 2.3 0.7 2.8 2.4 – 4.1 2.5 5.5 2.6 -1.5 -0.1 -0.7 0 – –
Endoplasmic Reticulum -4.8 0.7 -4.0 -4.8 – -2.8 -2.0 -5.0 -5.5 6.4 4.6 4.9 -4.6 – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Golgi -1.0 0.5 -4.6 -3.0 – -4.3 -4.9 -5.0 -5.5 -2.3 -3.5 -3.9 0 – –
Mitochondria 5.1 0 8.2 0 – 6.5 5.9 6.6 8.3 0 0 0 0 – –
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Nuclear Periphery -2.1 -4.1 -3.3 -2.5 – -3.3 -3.5 -3.3 -1.6 0.6 -0.1 -2.0 0 – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Vacuole -0.1 -1.7 -0.6 2.6 – -2.1 -2.9 -3.8 -2.2 -7.2 -7.2 -6.9 0 – –