Standard name
Human Ortholog
Description ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0.11 0.07 0.06 0 0.05 0.06 0.05 0.1 0 0 0 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.07 0 0.05 0 0.06 0 0.08 0.09 0.14 0.14 0.25 0 0 0 0 0 0
Cytoplasm 0 0.07 0.07 0.15 0.09 0.15 0.14 0.21 0.14 0.26 0.14 0 0.07 0.05 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0.76 0.6 0.64 0.37 0.67 0.44 0.41 0.5 0.54 0.4 0.39 0.92 0.84 0.85 0.06 0.84 0.8 0.87 0.9 0.83 0.86
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.11 0 0.13 0.09 0.15 0 0.05 0 0 0 0 0.06 0 0 0.31 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.13 0 0.28 0 0.19 0.16 0.19 0.27 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0.29 0.06 0.1 0 0 0 0 0 0 0 0.06 0.12 0.1 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.35 0.3 0.3 0.23 0.27 0.42 0.21 0.18 0.15 0.21 0 0 0.05 0.12 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 7 13 13 10 3 8 11 10 27 5 7 5 1 1 2 3 1 6 4
Bud 0 0 1 1 2 3 1 1 2 4 6 0 0 0 0 0 2 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 1 5 4 12 6 10 5 12 9 21 16 28 30 4 0 0 0 0 4 3
Cytoplasm 1 13 15 17 16 26 29 35 27 57 37 6 13 11 3 0 6 1 1 1 5
Endoplasmic Reticulum 42 114 142 43 124 74 87 84 104 87 102 162 166 177 1 81 188 226 114 165 186
Endosome 0 0 1 1 0 0 2 2 0 0 3 0 0 1 0 0 0 2 0 1 0
Golgi 6 3 29 11 27 1 10 2 1 2 2 11 7 6 5 3 3 4 0 3 4
Mitochondria 6 3 1 15 2 47 4 32 30 41 72 3 1 0 0 2 4 5 3 6 1
Nucleus 0 0 0 1 1 4 4 1 4 5 5 0 0 0 1 0 0 0 0 0 1
Nuclear Periphery 16 11 22 4 1 3 8 3 3 5 16 21 19 10 0 3 8 7 1 1 0
Nucleolus 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 10 66 67 35 42 46 89 35 34 33 56 4 7 10 2 0 13 5 0 3 5
Unique Cell Count 55 190 222 117 184 169 212 169 191 218 263 177 198 209 16 97 236 261 128 198 217
Labelled Cell Count 86 211 290 146 240 220 247 208 229 253 347 228 248 250 17 97 236 261 128 198 217


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 15.4 15.0 13.9 14.2 9.1 12.7 10.9 10.7 10.5 10.4 14.8 15.7 14.8 19.9 13.7 15.3 15.9
Std Deviation (1e-4) 3.3 3.0 2.7 3.2 2.4 2.4 2.7 2.3 2.3 2.5 2.1 2.4 2.8 2.2 4.3 2.4 2.9 2.9
Intensity Change (Log2) -0.11 -0.08 -0.72 -0.24 -0.46 -0.48 -0.51 -0.53 -0.01 0.06 -0.02 0.41 -0.13 0.03 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.0 1.8 1.3 0 0.8 1.3 0.8 3.1 -0.2 0.2 -0.5 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.1 0 1.4 0 2.1 1.1 2.8 3.0 4.5 4.6 0
Cytoplasm 2.3 0.7 2.8 2.4 4.1 2.5 5.5 2.6 -1.5 -0.1 -0.7 0
Endoplasmic Reticulum -4.8 0.7 -4.0 -4.8 -2.8 -2.0 -5.0 -5.5 6.4 4.6 4.9 -4.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.0 0.5 -4.6 -3.0 -4.3 -4.9 -5.0 -5.5 -2.3 -3.5 -3.9 0
Mitochondria 5.1 0 8.2 0 6.5 5.9 6.6 8.3 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.1 -4.1 -3.3 -2.5 -3.3 -3.5 -3.3 -1.6 0.6 -0.1 -2.0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 -1.7 -0.6 2.6 -2.1 -2.9 -3.8 -2.2 -7.2 -7.2 -6.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7458 9.8718 9.5005 9.4128 7.3841 9.8137 12.2165 13.5195 12.8676 13.3075 12.2201 12.4967 15.6688 16.1895 15.8998 15.2428 18.0904 16.4517
Actin 0.0375 0.0006 0.0072 0.0315 0.0035 0.008 0.0042 0.0002 0.0145 0.0004 0.0428 0.0102 0.0256 0.0004 0.0066 0.0001 0.0155 0.0026
Bud 0.0004 0.0001 0.0003 0.0003 0.0001 0.0001 0.0003 0 0.0002 0 0.0059 0.0001 0.0002 0 0.0002 0 0.0002 0
Bud Neck 0.0008 0.0003 0.0007 0.0003 0.0006 0.0003 0.0004 0.0001 0.0005 0.0001 0.0009 0.0006 0.0004 0 0.0033 0.0001 0.0043 0.0001
Bud Periphery 0.0007 0.0002 0.0003 0.0004 0.0002 0.0003 0.0005 0.0001 0.0002 0.0001 0.0112 0.0002 0.0003 0 0.0003 0.0001 0.0003 0.0001
Bud Site 0.0012 0.0002 0.0007 0.001 0.0007 0.0001 0.0019 0.0001 0.0052 0 0.0034 0.0001 0.0003 0 0.0146 0 0.0005 0
Cell Periphery 0.0007 0.0024 0.0003 0.0011 0.0003 0.0001 0.0007 0.0008 0.0006 0.0003 0.0009 0.0002 0.0003 0.0003 0.0009 0.0027 0.0003 0.0001
Cytoplasm 0.0108 0.0076 0.0093 0.0014 0.0064 0.0015 0.0106 0.0168 0.0143 0.0012 0.0027 0.0039 0.0054 0.0051 0.0229 0.0008 0.0481 0.0255
Cytoplasmic Foci 0.0117 0.0005 0.0083 0.0034 0.0106 0.0048 0.0099 0.0011 0.0132 0.001 0.0035 0.001 0.0105 0.0001 0.0034 0 0.0044 0.0006
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0.004 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.6752 0.917 0.8709 0.8619 0.7367 0.8629 0.7219 0.9418 0.8841 0.91 0.839 0.8781 0.7508 0.9787 0.9031 0.9264 0.8842 0.9219
Endosome 0.0387 0.0045 0.0136 0.0197 0.048 0.018 0.0507 0.0094 0.0173 0.0188 0.0219 0.0169 0.0164 0.0022 0.0031 0.0023 0.006 0.0037
Golgi 0.0179 0.0028 0.0098 0.0109 0.023 0.0093 0.0125 0.0015 0.0137 0.0169 0.0106 0.0098 0.0076 0.0007 0.0011 0.0002 0.0035 0.0007
Lipid Particles 0.0159 0.0059 0.0116 0.0309 0.0243 0.017 0.0078 0.0085 0.0103 0.0053 0.0318 0.004 0.0214 0.0004 0.0014 0.0033 0.0031 0.0024
Mitochondria 0.0107 0.0027 0.0149 0.006 0.0103 0.0123 0.01 0.0016 0.0036 0.0151 0.0084 0.0033 0.0041 0.0005 0.0017 0.0035 0.0003 0.0006
None 0.0048 0 0.0006 0.0001 0.0182 0.0002 0.002 0.0001 0.0004 0 0.0003 0.0002 0.0189 0 0.0047 0.0001 0.0003 0.0005
Nuclear Periphery 0.1445 0.0428 0.037 0.0189 0.0787 0.0576 0.1079 0.0117 0.0134 0.0243 0.012 0.0614 0.0972 0.0088 0.0248 0.0461 0.0178 0.0331
Nucleolus 0.0006 0 0.0003 0 0.0025 0.0001 0.0006 0 0.0001 0 0.0001 0 0.0011 0 0.0002 0 0.0002 0.0003
Nucleus 0.007 0.0018 0.0016 0.0005 0.0053 0.0017 0.0397 0.0008 0.0008 0.0016 0.0004 0.0038 0.0046 0.0003 0.001 0.0011 0.0007 0.0028
Peroxisomes 0.0025 0 0.0041 0.0019 0.002 0.0013 0.0044 0 0.0027 0.0002 0.0003 0.0002 0.0107 0 0.0008 0 0.0018 0.0003
Punctate Nuclear 0.0055 0.0001 0.0006 0.0002 0.0177 0.0005 0.0018 0.0001 0.0008 0 0.0006 0.0005 0.0129 0 0.0021 0 0.0063 0.0025
Vacuole 0.0064 0.008 0.0039 0.0051 0.0039 0.0021 0.0066 0.0046 0.0033 0.0027 0.0021 0.0031 0.003 0.0018 0.0018 0.0078 0.001 0.0011
Vacuole Periphery 0.0063 0.0022 0.0038 0.0043 0.0067 0.0018 0.0057 0.0006 0.0007 0.0018 0.0011 0.0023 0.0044 0.0005 0.0018 0.0053 0.0008 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.226 26.9012 6.6539 15.6207 32.5139 11.6963 20.7776 25.4972 15.4388 27.9091
Translational Efficiency 2.1147 1.479 2.9466 1.356 0.9805 2.923 1.9632 1.1005 1.7556 1.4396

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
70 790 268 1591 1640 1650 2394 205 1710 2440 2662 1796

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1119.82 1390.07 1822.00 1683.06 1091.48 1255.21 1607.27 2053.92 1092.64 1298.87 1628.89 1725.39
Standard Deviation 204.50 240.67 256.62 337.20 177.76 216.84 262.90 279.97 179.02 233.52 270.11 351.53
Intensity Change Log 2 0.311891 0.702256 0.587820 0.201643 0.558327 0.912094 0.258526 0.633007 0.756978

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001144 0.003850 0.000488 0.002068 0.000813 0.003725 0.001067 0.000483 0.000826 0.003766 0.001009 0.001887
Bud Neck 0.001708 0.000324 0.003690 0.003022 0.000208 0.001146 0.000571 0.007971 0.000270 0.000880 0.000885 0.003587
Bud Site 0.014233 0.005939 0.002479 0.012688 0.002978 0.008560 0.004097 0.010010 0.003439 0.007712 0.003934 0.012383
Cell Periphery 0.001502 0.001243 0.001604 0.002469 0.000743 0.001554 0.002570 0.003994 0.000774 0.001453 0.002472 0.002643
Cytoplasm 0.006290 0.003977 0.003050 0.024099 0.005388 0.028825 0.005870 0.001495 0.005425 0.020780 0.005586 0.021519
Cytoplasmic Foci 0.020809 0.006222 0.001608 0.011844 0.004247 0.031117 0.001717 0.001235 0.004925 0.023057 0.001706 0.010633
Eisosomes 0.000047 0.000063 0.000032 0.000075 0.000038 0.000148 0.000055 0.000056 0.000039 0.000120 0.000052 0.000073
Endoplasmic Reticulum 0.837456* 0.920125* 0.864430* 0.742957* 0.942912* 0.660160* 0.933086* 0.884391* 0.938595* 0.744329* 0.926174* 0.759101*
Endosome 0.021467 0.016936 0.015904 0.030236 0.009554 0.035354 0.011298 0.018103 0.010041 0.029391 0.011762 0.028851
Golgi 0.010299 0.008183 0.002184 0.013875 0.003618 0.026462 0.005982 0.004504 0.003891 0.020544 0.005600 0.012806
Lipid Particles 0.012120 0.003193 0.004811 0.032036 0.003421 0.031590 0.006882 0.017986 0.003777 0.022396 0.006674 0.030433
Mitochondria 0.015184 0.001142 0.001033 0.001663 0.001697 0.008161 0.000574 0.000278 0.002249 0.005889 0.000621 0.001505
Mitotic Spindle 0.001136 0.001741 0.000426 0.007972 0.000101 0.003984 0.000249 0.003154 0.000144 0.003258 0.000266 0.007422
None 0.000271 0.001661 0.000023 0.003063 0.002248 0.009539 0.001336 0.000329 0.002167 0.006988 0.001204 0.002751
Nuclear Periphery 0.000612 0.002428 0.036025 0.010276 0.004496 0.031502 0.001564 0.006157 0.004337 0.022089 0.005034 0.009806
Nuclear Periphery Foci 0.004606 0.013494 0.047337 0.067117 0.013740 0.082617 0.009895 0.018094 0.013366 0.060237 0.013664 0.061522
Nucleolus 0.001181 0.000068 0.000009 0.000450 0.000030 0.000794 0.000012 0.000024 0.000078 0.000559 0.000012 0.000402
Nucleus 0.035906 0.000252 0.001240 0.002728 0.000271 0.005094 0.000214 0.000198 0.001729 0.003526 0.000317 0.002439
Peroxisomes 0.000920 0.000342 0.000026 0.000294 0.000242 0.001594 0.000326 0.000052 0.000270 0.001189 0.000296 0.000267
Vacuole 0.010092 0.005469 0.012432 0.027838 0.001966 0.021507 0.010673 0.017323 0.002299 0.016314 0.010850 0.026638
Vacuole Periphery 0.003017 0.003347 0.001169 0.003226 0.001289 0.006566 0.001961 0.004162 0.001359 0.005524 0.001881 0.003333

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.64 1.25 -1.70 1.01 -3.95 -4.16 -0.62 1.01 4.61 1.30 -4.14 -0.49 -2.97 2.30 -2.02
Bud Neck 1.41 -1.50 -1.81 -9.54 0.35 -6.64 -3.61 -3.00 -2.67 -2.87 -5.60 -4.19 -8.59 -7.10 -6.85
Bud Site 0.88 1.25 0.05 -3.59 -5.93 -4.64 -1.39 -1.90 -0.58 -1.59 -3.91 -0.72 -6.24 -3.36 -5.88
Cell Periphery 0.45 -0.19 -2.02 -3.70 -1.55 -1.62 -4.98 -5.11 -3.15 -2.04 -1.90 -5.05 -8.09 -3.16 -0.57
Cytoplasm 0.77 0.87 -4.43 -8.71 -7.69 -11.35 -0.76 4.16 13.49 5.38 -10.05 -0.42 -8.03 -1.05 -7.96
Cytoplasmic Foci 1.62 2.11 1.03 -3.02 -5.44 -11.51 3.11 3.10 13.06 0.81 -10.18 3.70 -4.05 6.36 -7.59
Eisosomes -0.27 1.05 -1.08 -0.52 -2.45 -3.30 -0.72 -0.72 2.44 -0.06 -3.22 -0.64 -2.26 1.94 -1.12
Endoplasmic Reticulum -2.14 -0.74 2.38 17.33 8.28 30.36* 2.42 3.64 -10.88 2.97 25.21 2.78 21.60 -0.10 20.24
Endosome 0.55 0.65 -1.13 -7.52 -6.97 -15.15 -2.36 -3.11 4.91 -2.44 -14.43 -2.26 -13.78 -0.11 -12.76
Golgi 0.28 1.16 -0.51 -2.54 -6.41 -10.38 -2.39 -0.69 9.33 1.20 -9.93 -1.80 -5.92 3.94 -4.72
Lipid Particles 0.99 0.77 -2.09 -11.97 -9.08 -11.97 -3.73 -2.43 1.94 -1.85 -10.87 -3.06 -11.65 -3.34 -10.32
Mitochondria 1.10 1.10 1.05 -1.13 -0.78 -6.77 3.02 3.81 8.78 2.07 -4.20 2.55 1.01 6.28 -2.61
Mitotic Spindle -0.44 0.81 -4.78 -3.91 -5.88 -4.81 -1.58 -1.94 0.52 -1.84 -4.70 -1.36 -6.36 -3.42 -6.24
None -2.05 2.00 -3.26 -1.32 -3.63 -4.41 1.45 3.53 5.80 2.63 -4.05 1.63 -0.65 3.24 -1.90
Nuclear Periphery -4.58 -6.94 -8.55 -6.74 4.91 -11.28 5.12 -0.69 7.08 -1.78 -10.53 -0.99 -4.82 6.30 -4.07
Nuclear Periphery Foci -3.38 -6.83 -15.48 -13.02 -3.03 -16.01* 2.06 -1.00 9.54 -1.65 -14.74 -0.47 -13.54 -0.88 -13.60
Nucleolus 1.90 1.99 1.24 -2.04 -2.40 -4.04 2.33 0.34 4.06 -0.85 -3.64 2.48 -1.95 0.87 -2.40
Nucleus 1.86 1.80 1.71 -4.99 -1.35 -6.11 0.59 -0.15 6.04 -0.44 -1.83 1.71 -1.09 1.01 -4.74
Peroxisomes 1.66 2.57 1.89 0.20 -3.33 -6.47 -0.53 3.85 7.43 1.81 -5.79 -0.11 0.08 5.51 0.15
Vacuole 1.21 0.03 -2.96 -10.17 -5.27 -10.23 -9.20 -3.46 0.39 -1.61 -10.08 -9.10 -12.80 -5.58 -8.17
Vacuole Periphery -0.23 1.26 -0.17 0.14 -4.32 -7.92 -2.31 -1.45 1.13 -1.08 -7.75 -1.93 -4.35 3.38 -3.04